################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AvrII BalI BamHI BanI BanII BbeI BbsI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FbaI FseI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Alw44I 1084 GTGCAC G^TGCAC AocI 1118 CCTCAGG CC^T[ACGT]AGG ApaLI 1084 GTGCAC G^TGCAC AviII 743 TGCGCA TGC^GCA BbrPI 1393 CACGTG CAC^GTG BbvCI 1525 GCTGAGG CCTCAGC(-5/-2) BciVI 909 GGATAC GTATCC(6/5) BcnI 498 CCCGG CC^[GC]GG BfaI 1285 CTAG C^TAG BpmI 895 CTCCAG CTGGAG(16/14) BsaAI 1393 CACGTG [CT]AC^GT[GA] BsmAI 1016 GAGAC GTCTC(1/5) BsrBI 721 GAGCGG CCGCTC(-3/-3) BsrFI 842 ACCGGC [GA]^CCGG[CT] BstYI 1538 AGATCC [GA]^GATC[CT] Bsu36I 1118 CCTCAGG CC^T[ACGT]AGG Cfr10I 842 ACCGGC [GA]^CCGG[CT] DraI 850 TTTAAA TTT^AAA DraII 58 GGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 1393 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 83 CCATGG C^C[GA][CT]GG Eco81I 1118 CCTCAGG CC^T[ACGT]AGG EcoO109I 58 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 370 GATATC GAT^ATC FspI 743 TGCGCA TGC^GCA HindIII 1510 AAGCTT A^AGCTT MaeI 1285 CTAG C^TAG MflI 1538 AGATCC [GA]^GATC[CT] MseI 851 TTAA T^TAA NciI 498 CCCGG CC^[GC]GG NcoI 83 CCATGG C^CATGG PleI 1018 GACTC GAGTC(4/5) PmaCI 1393 CACGTG CAC^GTG PmlI 1393 CACGTG CAC^GTG PpuMI 58 GGGTCCT [GA]G^G[AT]CC[CT] SbfI 1269 CCTGCAGG CCTGCA^GG SmlI 178 CTTGAG C^T[CT][GA]AG Sse8387I 1269 CCTGCAGG CCTGCA^GG StuI 302 AGGCCT AGG^CCT Tru9I 851 TTAA T^TAA XbaI 1284 TCTAGA T^CTAGA XcmI 315 CCAGGCTGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1538 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 256 GTCTAC GT^[AC][GT]AC 1435 GTCTAC AcsI 187 GAATTC [GA]^AATT[CT] 411 GAATTC ApoI 187 GAATTC [GA]^AATT[CT] 411 GAATTC Asp700I 411 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1507 GAAAAGCTTC BcgI 970 CGATCCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1173 GCAGCTGGTTCG BglI 1116 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1268 GCCTGCAGGGC BsgI 531 CTGCAC GTGCAG(16/14) 954 CTGCAC CfoI 744 GCGC GCG^C 822 GCGC EcoRI 187 GAATTC G^AATTC 411 GAATTC FauI 792 CCCGC CCCGC(4/6) 732 GCGGG HhaI 744 GCGC GCG^C 822 GCGC HinP1I 744 GCGC G^CGC 822 GCGC HphI 1398 GGTGA GGTGA(8/7) 1079 TCACC PvuII 873 CAGCTG CAG^CTG 1174 CAGCTG TaqI 419 TCGA T^CGA 558 TCGA Tsp509I 188 AATT ^AATT 412 AATT TthHB8I 419 TCGA T^CGA 558 TCGA XmnI 411 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1507 GAAAAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BbvI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I TspRI