(Circular) (Six-base) MAPSORT of: pFN21AE2260.seq Check: 6228 from: 1 to: 6256 pFN21AE2260 6256 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2130 2142 2130 Size: 12 6244 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2262 3441 3599 1937 Size: 325 1179 158 4594 Fragments arranged by size: 4594 1179 325 158 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2950 3477 3480 4319 5703 5971 1632 Size: 1318 527 3 839 1384 268 1917 Fragments arranged by size: 1917 1384 1318 839 527 268 3 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 5155 6132 1203 Size: 3952 977 1327 Fragments arranged by size: 3952 1327 977 AgeI A'CCGG_T Cuts at: 5087 5087 Size: 6256 AhdI GACnn_n'nnGTC Cuts at: 4985 4985 Size: 6256 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6256 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2840 1184 Size: 1656 4600 AlwNI CAG_nnn'CTG Cuts at: 2122 2134 3217 3485 3999 5571 2122 Size: 12 1083 268 514 1572 2807 Fragments arranged by size: 2807 1572 1083 514 268 12 ApaLI G'TGCA_C Cuts at: 3089 4313 5469 3089 Size: 1224 1156 3876 Fragments arranged by size: 3876 1224 1156 ApoI r'AATT_y Cuts at: 1493 2192 2416 3565 3791 3943 6052 1493 Size: 699 224 1149 226 152 2109 1697 Fragments arranged by size: 2109 1697 1149 699 226 224 152 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 3581 1870 Size: 73 1638 4545 Fragments arranged by size: 4545 1638 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3028 3061 3028 Size: 33 6223 BamHI G'GATC_C Cuts at: 3586 3953 3586 Size: 367 5889 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3577 5032 618 Size: 325 217 627 1790 1455 1842 Fragments arranged by size: 1842 1790 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3575 1391 Size: 2184 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5285 BbvCI CC'TCA_GC Cuts at: 3531 3531 Size: 6256 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2964 2998 3167 3201 4447 Size: 34 50 34 943 34 169 34 1246 Cuts at: 4447 4481 1903 Size: 34 3678 Fragments arranged by size: 3678 1246 943 169 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2908 4148 5358 2908 Size: 1240 1210 3806 Fragments arranged by size: 3806 1240 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3127 3279 4867 Size: 107 122 71 631 605 1455 152 1588 Cuts at: 4867 136 Size: 1525 Fragments arranged by size: 1588 1525 1455 631 605 152 122 107 71 BglII A'GATC_T Cuts at: 6251 6251 Size: 6256 BlpI GC'TnA_GC Cuts at: 3673 3673 Size: 6256 Bme1580I G_kGCm'C Cuts at: 1790 2777 2974 3093 4317 5473 1790 Size: 987 197 119 1224 1156 2573 Fragments arranged by size: 2573 1224 1156 987 197 119 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3378 3473 3987 Size: 416 53 83 399 24 1961 95 514 Cuts at: 3987 4945 442 Size: 958 1753 Fragments arranged by size: 1961 1753 958 514 416 399 95 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2588 2588 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2885 4916 1239 Size: 1646 2031 2579 Fragments arranged by size: 2579 2031 1646 Bpu10I CC'TnA_GC Cuts at: 3486 3531 3486 Size: 45 6211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2204 4381 5246 5544 2204 Size: 2177 865 298 2916 Fragments arranged by size: 2916 2177 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4919 882 Size: 617 3420 2219 Fragments arranged by size: 3420 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3400 4047 493 Size: 711 2196 647 2702 Fragments arranged by size: 2702 2196 711 647 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4063 4445 5080 275 Size: 53 83 186 936 2530 382 635 1451 Fragments arranged by size: 2530 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4689 5087 5361 5508 5919 4689 Size: 398 274 147 411 5026 Fragments arranged by size: 5026 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 1178 Size: 30 165 30 624 30 5377 Fragments arranged by size: 5377 624 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 2168 2183 2942 3347 3548 1186 Size: 979 3 15 759 405 201 3894 Fragments arranged by size: 3894 979 759 405 201 15 3 BseYI C'CCAG_C Cuts at: 5459 5955 5459 Size: 496 5760 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2521 2944 1163 Size: 573 785 423 4475 Fragments arranged by size: 4475 785 573 423 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3706 4467 5495 1540 Size: 275 186 1705 761 1028 2301 Fragments arranged by size: 2301 1705 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2084 2409 2571 3093 4317 4402 5473 2084 Size: 325 162 522 1224 85 1071 2867 Fragments arranged by size: 2867 1224 1071 522 325 162 85 BsmI GAATG_Cn' Cuts at: 3767 3860 3767 Size: 93 6163 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2409 2571 2777 2974 3093 3575 Size: 399 294 325 162 206 197 119 482 Cuts at: 3575 4317 4402 5473 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 482 399 325 294 206 197 162 119 85 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2130 2142 2543 2801 3611 844 Size: 1286 12 401 258 810 3489 Fragments arranged by size: 3489 1286 810 401 258 12 BsrBI CCG'CTC Cuts at: 1394 1823 2728 4143 1394 Size: 429 905 1415 3507 Fragments arranged by size: 3507 1415 905 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4751 4925 65 Size: 1183 3503 174 1396 Fragments arranged by size: 3503 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2847 4900 5087 1700 Size: 110 1037 2053 187 2869 Fragments arranged by size: 2869 2053 1037 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6256 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6256 BssSI C'ACGA_G Cuts at: 4316 5328 4316 Size: 1012 5244 BstAPI GCAn_nnn'nTGC Cuts at: 794 2134 2311 3220 794 Size: 1340 177 909 3830 Fragments arranged by size: 3830 1340 909 177 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6256 BstYI r'GATC_y Cuts at: 1972 3543 3586 3953 4338 4355 5807 5893 5905 Size: 1571 43 367 385 17 1452 86 12 Cuts at: 5905 6251 1972 Size: 346 1977 Fragments arranged by size: 1977 1571 1452 385 367 346 86 43 17 12 Bsu36I CC'TnA_GG Cuts at: 3124 3124 Size: 6256 BtgI C'CryG_G Cuts at: 513 1065 2088 513 Size: 552 1023 4681 Fragments arranged by size: 4681 1023 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5517 Fragments arranged by size: 5517 683 56 BtsI GCAGTG_nn' Cuts at: 2269 3772 4566 4586 2269 Size: 1503 794 20 3939 Fragments arranged by size: 3939 1503 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 2857 3560 2857 Size: 703 5553 DraIII CAC_nnn'GTG Cuts at: 3403 3403 Size: 6256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3703 4592 6221 Size: 54 1425 50 275 18 1873 889 1629 Cuts at: 6221 8 Size: 43 Fragments arranged by size: 1873 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3703 1537 Size: 275 1891 4090 Fragments arranged by size: 4090 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 2160 3318 3324 4186 1353 Size: 801 6 1158 6 862 3423 Fragments arranged by size: 3423 1158 862 801 6 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2243 4843 5217 5363 387 Size: 1064 138 654 2600 374 146 1280 Fragments arranged by size: 2600 1280 1064 654 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2885 2950 3477 3480 4319 4916 5703 Size: 393 1253 65 527 3 839 597 787 Cuts at: 5703 5971 1239 Size: 268 1524 Fragments arranged by size: 1524 1253 839 787 597 527 393 268 65 3 EcoO109I rG'GnC_Cy Cuts at: 2064 2064 Size: 6256 EcoRI G'AATT_C Cuts at: 2192 2416 3565 3943 2192 Size: 224 1149 378 4505 Fragments arranged by size: 4505 1149 378 224 EcoRV GAT'ATC Cuts at: 2377 2377 Size: 6256 FspI TGC'GCA Cuts at: 2750 4762 2750 Size: 2012 4244 HaeII r_GCGC'y Cuts at: 1678 5403 1678 Size: 3725 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 4897 4929 4971 Size: 32 135 32 476 32 2817 32 42 Cuts at: 4971 5003 1373 Size: 32 2626 Fragments arranged by size: 2817 2626 476 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3600 3846 669 Size: 988 281 1662 246 3079 Fragments arranged by size: 3079 1662 988 281 246 HindIII A'AGCT_T Cuts at: 748 3515 748 Size: 2767 3489 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5370 5554 1305 Size: 4065 184 2007 Fragments arranged by size: 4065 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4434 Fragments arranged by size: 4434 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2136 3051 3159 3398 4214 4573 4732 Size: 648 970 915 108 239 816 359 159 Cuts at: 4732 518 Size: 2042 Fragments arranged by size: 2042 970 915 816 648 359 239 159 108 MspA1I CmG'CkG Cuts at: 1606 2800 2880 3181 3672 4349 5497 5742 1606 Size: 1194 80 301 491 677 1148 245 2120 Fragments arranged by size: 2120 1194 1148 677 491 301 245 80 NcoI C'CATG_G Cuts at: 513 1065 2088 513 Size: 552 1023 4681 Fragments arranged by size: 4681 1023 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 2111 2453 2626 2749 2827 3614 5159 Size: 97 882 342 173 123 78 787 1545 Cuts at: 5159 1132 Size: 2229 Fragments arranged by size: 2229 1545 882 787 342 173 123 97 78 PasI CC'CwG_GG Cuts at: 1348 3464 1348 Size: 2116 4140 PciI A'CATG_T Cuts at: 5155 5155 Size: 6256 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6256 PfoI T'CCnGG_A Cuts at: 2343 2343 Size: 6256 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PmlI CAC'GTG Cuts at: 3400 3400 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2064 2064 Size: 6256 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PstI C_TGCA'G Cuts at: 830 2127 2307 3180 3279 3608 830 Size: 1297 180 873 99 329 3478 Fragments arranged by size: 3478 1297 873 329 180 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 PvuII CAG'CTG Cuts at: 2880 3181 2880 Size: 301 5955 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SbfI CC_TGCA'GG Cuts at: 3279 3608 3279 Size: 329 5927 ScaI AGT'ACT Cuts at: 4504 6216 4504 Size: 1712 4544 SfcI C'TryA_G Cuts at: 826 1045 2048 2123 2303 2875 2938 3176 3275 Size: 219 1003 75 180 572 63 238 99 Cuts at: 3275 3604 4739 5420 5611 826 Size: 329 1135 681 191 1471 Fragments arranged by size: 1471 1135 1003 681 572 329 238 219 191 180 99 75 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 3583 1872 Size: 1711 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2183 4360 5261 5523 820 Size: 197 926 240 2177 901 262 1553 Fragments arranged by size: 2177 1553 926 901 262 240 197 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 3614 1132 Size: 2482 3774 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 2309 1517 Size: 792 5464 StyI C'CwwG_G Cuts at: 513 1065 2088 2329 3032 3421 3695 513 Size: 552 1023 241 703 389 274 3074 Fragments arranged by size: 3074 1023 703 552 389 274 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4283 4300 4453 4638 5083 325 Size: 1255 2703 17 153 185 445 1498 Fragments arranged by size: 2703 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3010 4502 6214 Size: 274 80 33 51 1443 1033 1492 1712 Cuts at: 6214 96 Size: 138 Fragments arranged by size: 1712 1492 1443 1033 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2027 2059 6216 6248 2027 Size: 32 4157 32 2035 Fragments arranged by size: 4157 2035 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6256 XbaI T'CTAG_A Cuts at: 3289 3592 3289 Size: 303 5953 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2327 2327 Size: 6256 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6256 XmaI C'CCGG_G Cuts at: 1870 3581 1870 Size: 1711 4545 XmnI GAAnn'nnTTC Cuts at: 2420 3516 4385 2420 Size: 1096 869 4291 Fragments arranged by size: 4291 1096 869 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI