################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII AvrII BamHI BbrPI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FauI FseI FspI HgaI HincII HindII HindIII HpaI KspI MaeII MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 728 GGTACC G^GTACC AccI 511 GTCTAC GT^[AC][GT]AC AccII 116 CGCG CG^CG AcyI 365 GGCGCC G[GA]^CG[CT]C ApaI 1114 GGGCCC GGGCC^C Asp718I 728 GGTACC G^GTACC AvaII 132 GGACC G^G[AT]CC BanII 1114 GGGCCC G[GA]GC[CT]^C BbeI 365 GGCGCC GGCGC^C BbsI 619 GTCTTC GAAGAC(2/6) BbvCI 871 CCTCAGC CCTCAGC(-5/-2) BpuAI 619 GTCTTC GAAGAC(2/6) BsaHI 365 GGCGCC G[GA]^CG[CT]C BseRI 406 CTCCTC GAGGAG(10/8) BsmBI 48 CGTCTC CGTCTC(1/5) BsmFI 804 GGGAC GGGAC(10/14) BsmI 265 GCATTC GAATGC(1/-1) BspHI 680 TCATGA T^CATGA BssHII 115 GCGCGC G^CGCGC BstUI 116 CGCG CG^CG DdeI 872 CTCAG C^T[ACGT]AG DraI 999 TTTAAA TTT^AAA DraII 131 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 788 CCGTGG C^C[GA][CT]GG EcoO109I 131 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 1060 GAATTC G^AATTC EcoRV 1193 GATATC GAT^ATC EcoT14I 1110 CCTTGG C^C[AT][AT]GG HaeII 365 GGCGCC [GA]GCGC^[CT] Hin1I 365 GGCGCC G[GA]^CG[CT]C KasI 365 GGCGCC G^GCGCC KpnI 728 GGTACC GGTAC^C MvnI 116 CGCG CG^CG NarI 365 GGCGCC GG^CGCC PpuMI 131 AGGACCT [GA]G^G[AT]CC[CT] PstI 1156 CTGCAG CTGCA^G RcaI 680 TCATGA T^CATGA SexAI 626 ACCTGGT A^CC[AT]GGT SfoI 365 GGCGCC GGC^GCC SpeI 594 ACTAGT A^CTAGT StyI 1110 CCTTGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 280 GAATTT [GA]^AATT[CT] 1060 GAATTC AlwI 159 GATCC GGATC(4/5) 1161 GATCC AlwNI 874 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 1159 CAGATCCTG ApoI 280 GAATTT [GA]^AATT[CT] 1060 GAATTC BalI 68 TGGCCA TGG^CCA 585 TGGCCA BcnI 87 CCCGG CC^[GC]GG 96 CCGGG BpmI 1018 CTCCAG CTGGAG(16/14) 940 CTGGAG BsmAI 1045 GAGAC GTCTC(1/5) 49 GTCTC BsrDI 108 CATTGC GCAATG(2/0) 1139 CATTGC BstYI 158 AGATCC [GA]^GATC[CT] 1160 AGATCC EaeI 68 TGGCCA [CT]^GGCC[GA] 585 TGGCCA EarI 208 CTCTTC CTCTTC(1/4) 1210 CTCTTC HapII 88 CCGG C^CGG 96 CCGG HpaII 88 CCGG C^CGG 96 CCGG Ksp632I 208 CTCTTC CTCTTC(1/4) 1210 CTCTTC MflI 158 AGATCC [GA]^GATC[CT] 1160 AGATCC MluNI 68 TGGCCA TGG^CCA 585 TGGCCA MscI 68 TGGCCA TGG^CCA 585 TGGCCA MslI 444 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1036 CACCAGAGTG MspI 88 CCGG C^CGG 96 CCGG NciI 87 CCCGG CC^[GC]GG 96 CCGGG PleI 917 GACTC GAGTC(4/5) 1208 GACTC PvuII 389 CAGCTG CAG^CTG 1021 CAGCTG SfaNI 483 GCATC GCATC(5/9) 797 GCATC SfcI 386 CTACAG C^T[GA][CT]AG 1156 CTGCAG TaqI 1191 TCGA T^CGA 1206 TCGA TthHB8I 1191 TCGA T^CGA 1206 TCGA XhoII 158 AGATCC [GA]^GATC[CT] 1160 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AfaI AluI BanI BbvI BclI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII EcoRII FbaI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp45I Tsp509I TspRI