(Circular) (Six-base) MAPSORT of: pFN21AE3442.seq Check: 6869 from: 1 to: 8668 pFN21AE3442 8668 bp With 182 enzymes: SgfI * October 21, 2016 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3496 3496 Size: 8668 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6478 278 Size: 53 83 186 936 4942 2468 Fragments arranged by size: 4942 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6011 1937 Size: 4074 4594 Acc65I G'GTAC_C Cuts at: 4897 5592 5989 4897 Size: 695 397 7576 Fragments arranged by size: 7576 695 397 AclI AA'CG_TT Cuts at: 1563 6795 7168 1563 Size: 5232 373 3063 Fragments arranged by size: 5232 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2681 3393 3719 4109 4213 5929 6731 8115 Size: 1049 712 326 390 104 1716 802 1384 Cuts at: 8115 8383 1632 Size: 268 1917 Fragments arranged by size: 1917 1716 1384 1049 802 712 390 326 268 104 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8471 AflIII A'CryG_T Cuts at: 1203 5897 7567 8544 1203 Size: 4694 1670 977 1327 Fragments arranged by size: 4694 1670 1327 977 AgeI A'CCGG_T Cuts at: 5393 7499 5393 Size: 2106 6562 AhdI GACnn_n'nnGTC Cuts at: 7397 7397 Size: 8668 AleI CACnn'nnGTG Cuts at: 1166 3129 1166 Size: 1963 6705 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4402 4422 4617 1184 Size: 3218 20 195 5235 Fragments arranged by size: 5235 3218 195 20 AlwNI CAG_nnn'CTG Cuts at: 2659 2815 3094 3260 3694 3697 4855 5056 5188 Size: 156 279 166 434 3 1158 201 132 Cuts at: 5188 6411 7983 2659 Size: 1223 1572 3344 Fragments arranged by size: 3344 1572 1223 1158 434 279 201 166 156 132 3 ApaI G_GGCC'C Cuts at: 2070 2730 2824 3638 5638 2070 Size: 660 94 814 2000 5100 Fragments arranged by size: 5100 2000 814 660 94 ApaLI G'TGCA_C Cuts at: 4806 6725 7881 4806 Size: 1919 1156 5593 Fragments arranged by size: 5593 1919 1156 ApoI r'AATT_y Cuts at: 1493 2581 3337 4068 4083 5977 6203 6355 8464 Size: 1088 756 731 15 1894 226 152 2109 Cuts at: 8464 1493 Size: 1697 Fragments arranged by size: 2109 1894 1697 1088 756 731 226 152 15 AseI AT'TA_AT Cuts at: 160 1032 7222 160 Size: 872 6190 1606 Fragments arranged by size: 6190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8668 AvaI C'yCGr_G Cuts at: 1870 1943 3207 4701 5588 5631 5993 1870 Size: 73 1264 1494 887 43 362 4545 Fragments arranged by size: 4545 1494 1264 887 362 73 43 BamHI G'GATC_C Cuts at: 5998 6365 5998 Size: 367 8301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2780 4575 4897 5503 5592 Size: 325 217 627 993 1795 322 606 89 Cuts at: 5592 5989 7444 618 Size: 397 1455 1842 Fragments arranged by size: 1842 1795 1455 993 627 606 397 325 322 217 89 BanII G_rGCy'C Cuts at: 1391 2070 2730 2824 3214 3638 3779 3815 5589 Size: 679 660 94 390 424 141 36 1774 Cuts at: 5589 5638 5987 1391 Size: 49 349 4072 Fragments arranged by size: 4072 1774 679 660 424 390 349 141 94 49 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2669 4028 5760 928 Size: 971 770 1359 1732 3836 Fragments arranged by size: 3836 1732 1359 971 770 BbvCI CC'TCA_GC Cuts at: 4107 4565 4107 Size: 458 8210 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6859 6893 1903 Size: 34 4922 34 3678 Fragments arranged by size: 4922 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5448 6560 7770 5448 Size: 1112 1210 6346 Fragments arranged by size: 6346 1210 1112 BclI T'GATC_A Cuts at: 1548 3121 3294 1548 Size: 1573 173 6922 Fragments arranged by size: 6922 1573 173 BfrBI ATG'CAT Cuts at: 5275 5275 Size: 8668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3642 7279 Size: 107 122 71 631 605 337 1633 3637 Cuts at: 7279 136 Size: 1525 Fragments arranged by size: 3637 1633 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2934 3448 8663 2934 Size: 514 5215 2939 Fragments arranged by size: 5215 2939 514 BlpI GC'TnA_GC Cuts at: 4457 5918 6085 4457 Size: 1461 167 7040 Fragments arranged by size: 7040 1461 167 Bme1580I G_kGCm'C Cuts at: 1790 2070 2730 2785 2824 3638 4578 4810 5206 Size: 280 660 55 39 814 940 232 396 Cuts at: 5206 5508 5638 6729 7885 1790 Size: 302 130 1091 1156 2573 Fragments arranged by size: 2573 1156 1091 940 814 660 396 302 280 232 130 55 39 BmgBI CAC'GTC Cuts at: 1318 2501 4836 1318 Size: 1183 2335 5150 Fragments arranged by size: 5150 2335 1183 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2064 4233 4297 Size: 416 53 83 399 24 647 2169 64 Cuts at: 4297 4509 4797 6399 7357 442 Size: 212 288 1602 958 1753 Fragments arranged by size: 2169 1753 1602 958 647 416 399 288 212 83 64 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8668 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2777 3793 3877 5112 5317 2777 Size: 1016 84 1235 205 6128 Fragments arranged by size: 6128 1235 1016 205 84 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2764 3498 4037 4058 4154 4471 5535 5624 Size: 1525 734 539 21 96 317 1064 89 Cuts at: 5624 7328 1239 Size: 1704 2579 Fragments arranged by size: 2579 1704 1525 1064 734 539 317 96 89 21 Bpu10I CC'TnA_GC Cuts at: 4107 4565 4107 Size: 458 8210 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3596 5407 6793 7658 7956 3596 Size: 1811 1386 865 298 4308 Fragments arranged by size: 4308 1811 1386 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7331 882 Size: 617 5832 2219 Fragments arranged by size: 5832 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2767 5898 6459 493 Size: 711 1563 3131 561 2702 Fragments arranged by size: 3131 2702 1563 711 561 BsaBI GATnn'nnATC Cuts at: 2598 8662 2598 Size: 6064 2604 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2967 6475 6857 7492 Size: 53 83 186 936 1434 3508 382 635 Cuts at: 7492 275 Size: 1451 Fragments arranged by size: 3508 1451 1434 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5393 7101 7499 7773 7920 8331 5393 Size: 1708 398 274 147 411 5730 Fragments arranged by size: 5730 1708 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3812 3842 4783 4813 1178 Size: 30 165 30 2409 30 941 30 5033 Fragments arranged by size: 5033 2409 941 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3415 3678 3785 4095 4356 5306 5869 1186 Size: 2229 263 107 310 261 950 563 3985 Fragments arranged by size: 3985 2229 950 563 310 263 261 107 BseYI C'CCAG_C Cuts at: 2107 2155 2304 3687 4026 4712 5099 5181 5507 Size: 48 149 1383 339 686 387 82 326 Cuts at: 5507 5596 5717 7871 8367 2107 Size: 89 121 2154 496 2408 Fragments arranged by size: 2408 2154 1383 686 496 387 339 326 149 121 89 82 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2676 3308 3824 3827 4632 1163 Size: 573 940 632 516 3 805 5199 Fragments arranged by size: 5199 940 805 632 573 516 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2139 2212 6118 6879 7907 1540 Size: 275 186 138 73 3906 761 1028 2301 Fragments arranged by size: 3906 2301 1028 761 275 186 138 73 BsiHKAI G_wGCw'C Cuts at: 3193 3779 3815 4810 5622 5844 6729 6814 7885 Size: 586 36 995 812 222 885 85 1071 Cuts at: 7885 3193 Size: 3976 Fragments arranged by size: 3976 1071 995 885 812 586 222 85 36 BsmI GAATG_Cn' Cuts at: 3005 4933 5275 5486 6179 6272 3005 Size: 1928 342 211 693 93 5401 Fragments arranged by size: 5401 1928 693 342 211 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3188 3188 Size: 8668 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2730 2785 2824 3193 3214 3638 Size: 399 280 660 55 39 369 21 424 Cuts at: 3638 3779 3815 4578 4810 5206 5508 5589 5622 Size: 141 36 763 232 396 302 81 33 Cuts at: 5622 5638 5844 5987 6729 6814 7885 1391 Size: 16 206 143 742 85 1071 2174 Fragments arranged by size: 2174 1071 763 742 660 424 399 396 369 302 280 232 206 143 141 85 81 55 39 36 33 21 16 BspEI T'CCGG_A Cuts at: 8331 8331 Size: 8668 BspHI T'CATG_A Cuts at: 5836 6557 8287 5836 Size: 721 1730 6217 Fragments arranged by size: 6217 1730 721 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3245 3496 4394 4411 4885 6023 844 Size: 2401 251 898 17 474 1138 3489 Fragments arranged by size: 3489 2401 1138 898 474 251 17 BsrBI CCG'CTC Cuts at: 1394 1823 2025 6555 1394 Size: 429 202 4530 3507 Fragments arranged by size: 4530 3507 429 202 BsrDI GCAATG_nn' Cuts at: 65 1248 7163 7337 65 Size: 1183 5915 174 1396 Fragments arranged by size: 5915 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2138 3873 4943 5263 5393 7312 7499 Size: 110 328 1735 1070 320 130 1919 187 Cuts at: 7499 1700 Size: 2869 Fragments arranged by size: 2869 1919 1735 1070 328 320 187 130 110 BsrGI T'GTAC_A Cuts at: 96 5680 96 Size: 5584 3084 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8668 BssSI C'ACGA_G Cuts at: 5302 5711 6728 7740 5302 Size: 409 1017 1012 6230 Fragments arranged by size: 6230 1017 1012 409 BstAPI GCAn_nnn'nTGC Cuts at: 794 2815 3260 3556 4855 794 Size: 2021 445 296 1299 4607 Fragments arranged by size: 4607 2021 1299 445 296 BstBI TT'CG_AA Cuts at: 7472 7472 Size: 8668 BstEII G'GTnAC_C Cuts at: 1184 4423 1184 Size: 3239 5429 BstXI CCAn_nnnn'nTGG Cuts at: 4555 4555 Size: 8668 BstYI r'GATC_y Cuts at: 1972 2905 2934 3448 5998 6365 6750 6767 8219 Size: 933 29 514 2550 367 385 17 1452 Cuts at: 8219 8305 8317 8663 1972 Size: 86 12 346 1977 Fragments arranged by size: 2550 1977 1452 933 514 385 367 346 86 29 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 2170 2473 2925 3060 3707 Size: 552 939 66 100 303 452 135 647 Cuts at: 3707 5245 513 Size: 1538 3936 Fragments arranged by size: 3936 1538 939 647 552 452 303 135 100 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7929 Fragments arranged by size: 7929 683 56 BtsI GCAGTG_nn' Cuts at: 2804 3092 3750 4270 4333 5568 6184 6978 6998 Size: 288 658 520 63 1235 616 794 20 Cuts at: 6998 2804 Size: 4474 Fragments arranged by size: 4474 1235 794 658 616 520 288 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4719 4754 529 Size: 35 4155 35 4443 Fragments arranged by size: 4443 4155 35 35 DraI TTT'AAA Cuts at: 2571 5874 5972 2571 Size: 3303 98 5267 Fragments arranged by size: 5267 3303 98 DraIII CAC_nnn'GTG Cuts at: 2467 3412 2467 Size: 945 7723 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3097 7675 809 Size: 507 1781 4578 1802 Fragments arranged by size: 4578 1802 1781 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2140 3057 Size: 54 1425 50 275 18 306 4 917 Cuts at: 3057 3643 4557 4945 6115 7004 8633 8 Size: 586 914 388 1170 889 1629 43 Fragments arranged by size: 1629 1425 1170 917 914 889 586 388 306 275 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2136 6115 1537 Size: 275 324 3979 4090 Fragments arranged by size: 4090 3979 324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2645 3354 4128 4232 6598 1353 Size: 1292 709 774 104 2366 3423 Fragments arranged by size: 3423 2366 1292 774 709 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2079 2876 3260 7255 7629 7775 Size: 1064 138 490 797 384 3995 374 146 Cuts at: 7775 387 Size: 1280 Fragments arranged by size: 3995 1280 1064 797 490 384 374 146 138 EcoICRI GAG'CTC Cuts at: 3777 3813 3777 Size: 36 8632 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2681 2764 3393 3498 3719 4037 4058 Size: 393 1049 83 629 105 221 318 21 Cuts at: 4058 4109 4154 4213 4471 5535 5624 5929 6731 Size: 51 45 59 258 1064 89 305 802 Cuts at: 6731 7328 8115 8383 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1064 1049 802 787 629 597 393 318 305 268 258 221 105 89 83 59 51 45 21 EcoNI CCTnn'n_nnAGG Cuts at: 3041 4731 5523 3041 Size: 1690 792 6186 Fragments arranged by size: 6186 1690 792 EcoO109I rG'GnC_Cy Cuts at: 2103 3635 3713 4852 5407 5628 5635 2103 Size: 1532 78 1139 555 221 7 5136 Fragments arranged by size: 5136 1532 1139 555 221 78 7 EcoRI G'AATT_C Cuts at: 5977 6355 5977 Size: 378 8290 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2248 4937 5341 2248 Size: 2689 404 5575 Fragments arranged by size: 5575 2689 404 FseI GG_CCGG'CC Cuts at: 2142 2142 Size: 8668 FspI TGC'GCA Cuts at: 7174 7174 Size: 8668 HaeII r_GCGC'y Cuts at: 1678 2920 3706 5131 7815 1678 Size: 1242 786 1425 2684 2531 Fragments arranged by size: 2684 2531 1425 1242 786 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2404 2436 3657 3689 4264 Size: 32 135 32 832 32 1221 32 575 Cuts at: 4264 4296 5399 5431 5505 5537 7309 7341 7383 Size: 32 1103 32 74 32 1772 32 42 Cuts at: 7383 7415 1373 Size: 32 2626 Fragments arranged by size: 2626 1772 1221 1103 832 575 135 74 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3484 4450 6012 6258 669 Size: 988 281 1546 966 1562 246 3079 Fragments arranged by size: 3079 1562 1546 988 966 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8668 HpaI GTT'AAC Cuts at: 6258 6258 Size: 8668 KpnI G_GTAC'C Cuts at: 4901 5596 5993 4901 Size: 695 397 7576 Fragments arranged by size: 7576 695 397 MfeI C'AATT_G Cuts at: 6267 6267 Size: 8668 MluI A'CGCG_T Cuts at: 8544 8544 Size: 8668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2483 2528 3700 3855 4775 5727 7782 7966 Size: 1178 45 1172 155 920 952 2055 184 Cuts at: 7966 1305 Size: 2007 Fragments arranged by size: 2055 2007 1178 1172 952 920 184 155 45 MscI TGG'CCA Cuts at: 10 64 1489 1832 3059 4559 10 Size: 54 1425 343 1227 1500 4119 Fragments arranged by size: 4119 1500 1425 1227 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3129 5209 5250 5463 5896 6626 6985 Size: 648 1963 2080 41 213 433 730 359 Cuts at: 6985 7144 518 Size: 159 2042 Fragments arranged by size: 2080 2042 1963 730 648 433 359 213 159 41 MspA1I CmG'CkG Cuts at: 1606 2072 2120 2172 2308 2528 2656 3292 3619 Size: 466 48 52 136 220 128 636 327 Cuts at: 3619 3694 3955 3976 4478 4597 5686 6084 6761 Size: 75 261 21 502 119 1089 398 677 Cuts at: 6761 7909 8154 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1089 677 636 502 466 398 327 261 245 220 136 128 119 75 52 48 21 NaeI GCC'GGC Cuts at: 2140 5265 2140 Size: 3125 5543 NcoI C'CATG_G Cuts at: 513 1065 2004 2473 2925 3060 513 Size: 552 939 469 452 135 6121 Fragments arranged by size: 6121 939 552 469 452 135 NdeI CA'TA_TG Cuts at: 387 2892 4038 387 Size: 2505 1146 5017 Fragments arranged by size: 5017 2505 1146 NgoMIV G'CCGG_C Cuts at: 2138 5263 2138 Size: 3125 5543 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8668 NotI GC'GGCC_GC Cuts at: 6115 6115 Size: 8668 NsiI A_TGCA'T Cuts at: 5277 5277 Size: 8668 NspI r_CATG'y Cuts at: 1132 1229 3205 3686 4137 4456 4468 6026 7571 Size: 97 1976 481 451 319 12 1558 1545 Cuts at: 7571 1132 Size: 2229 Fragments arranged by size: 2229 1976 1558 1545 481 451 319 97 12 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8668 PciI A'CATG_T Cuts at: 7567 7567 Size: 8668 PflMI CCAn_nnn'nTGG Cuts at: 1108 2398 2467 2795 2896 3316 5449 1108 Size: 1290 69 328 101 420 2133 4327 Fragments arranged by size: 4327 2133 1290 420 328 101 69 PfoI T'CCnGG_A Cuts at: 2337 3475 5551 2337 Size: 1138 2076 5454 Fragments arranged by size: 5454 2076 1138 PmeI GTTT'AAAC Cuts at: 5972 5972 Size: 8668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4346 4378 6737 6769 8250 8282 4346 Size: 32 2359 32 1481 32 4732 Fragments arranged by size: 4732 2359 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2103 3713 5407 2103 Size: 1610 1694 5364 Fragments arranged by size: 5364 1694 1610 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8668 PsiI TTA'TAA Cuts at: 6238 6238 Size: 8668 PspOMI G'GGCC_C Cuts at: 2066 2726 2820 3634 5634 2066 Size: 660 94 814 2000 5100 Fragments arranged by size: 5100 2000 814 660 94 PstI C_TGCA'G Cuts at: 830 2811 3099 3456 3507 3960 3981 4483 6020 Size: 1981 288 357 51 453 21 502 1537 Cuts at: 6020 830 Size: 3478 Fragments arranged by size: 3478 1981 1537 502 453 357 288 51 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8668 PvuII CAG'CTG Cuts at: 2308 2528 2656 3292 3619 3694 3955 3976 4478 Size: 220 128 636 327 75 261 21 502 Cuts at: 4478 4597 5686 2308 Size: 119 1089 5290 Fragments arranged by size: 5290 1089 636 502 327 261 220 128 119 75 21 SacI G_AGCT'C Cuts at: 3779 3815 3779 Size: 36 8632 SacII CC_GC'GG Cuts at: 2073 2173 2073 Size: 100 8568 SalI G'TCGA_C Cuts at: 1936 6010 1936 Size: 4074 4594 SanDI GG'GwC_CC Cuts at: 2103 3713 5407 2103 Size: 1610 1694 5364 Fragments arranged by size: 5364 1694 1610 SapI GCTCTTCn'nnn_ Cuts at: 3354 3354 Size: 8668 SbfI CC_TGCA'GG Cuts at: 3507 6020 3507 Size: 2513 6155 ScaI AGT'ACT Cuts at: 6916 8628 6916 Size: 1712 6956 SexAI A'CCwGG_T Cuts at: 2391 2463 2666 2391 Size: 72 203 8393 Fragments arranged by size: 8393 203 72 SfcI C'TryA_G Cuts at: 826 1045 2807 3095 3127 3139 3452 3503 3599 Size: 219 1762 288 32 12 313 51 96 Cuts at: 3599 3614 3956 3977 4479 4720 4844 6016 7151 Size: 15 342 21 502 241 124 1172 1135 Cuts at: 7151 7832 8023 826 Size: 681 191 1471 Fragments arranged by size: 1762 1471 1172 1135 681 502 342 313 288 241 219 191 124 96 51 32 21 15 12 SfiI GGCCn_nnn'nGGCC Cuts at: 3642 3642 Size: 8668 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8668 SmaI CCC'GGG Cuts at: 1872 4703 5633 5995 1872 Size: 2831 930 362 4545 Fragments arranged by size: 4545 2831 930 362 SmlI C'TyrA_G Cuts at: 820 1017 1943 3575 5386 6772 7673 7935 820 Size: 197 926 1632 1811 1386 901 262 1553 Fragments arranged by size: 1811 1632 1553 1386 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6459 493 Size: 5966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8668 SphI G_CATG'C Cuts at: 1132 3205 3686 6026 1132 Size: 2073 481 2340 3774 Fragments arranged by size: 3774 2340 2073 481 SspI AAT'ATT Cuts at: 5 52 2454 6592 5 Size: 47 2402 4138 2081 Fragments arranged by size: 4138 2402 2081 47 StuI AGG'CCT Cuts at: 1517 3905 1517 Size: 2388 6280 StyI C'CwwG_G Cuts at: 513 1065 2004 2268 2274 2473 2749 2925 3060 Size: 552 939 264 6 199 276 176 135 Cuts at: 3060 3640 5074 5104 5316 6107 513 Size: 580 1434 30 212 791 3074 Fragments arranged by size: 3074 1434 939 791 580 552 276 264 212 199 176 135 30 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3404 5248 5859 6695 6712 6865 7050 Size: 1255 1824 1844 611 836 17 153 185 Cuts at: 7050 7495 325 Size: 445 1498 Fragments arranged by size: 1844 1824 1498 1255 836 611 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3579 5348 5680 Size: 274 80 33 51 1443 1602 1769 332 Cuts at: 5680 6914 8626 96 Size: 1234 1712 138 Fragments arranged by size: 1769 1712 1602 1443 1234 332 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4783 4815 5288 5320 5547 5579 8628 8660 4783 Size: 32 473 32 227 32 3049 32 4791 Fragments arranged by size: 4791 3049 473 227 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8668 XbaI T'CTAG_A Cuts at: 6004 6004 Size: 8668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3067 3067 Size: 8668 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8668 XmaI C'CCGG_G Cuts at: 1870 4701 5631 5993 1870 Size: 2831 930 362 4545 Fragments arranged by size: 4545 2831 930 362 XmnI GAAnn'nnTTC Cuts at: 2333 4640 6797 2333 Size: 2307 2157 4204 Fragments arranged by size: 4204 2307 2157 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6476 276 Size: 53 83 186 936 4942 2468 Fragments arranged by size: 4942 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I EcoRV FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SfoI SrfI SwaI