Restriction map for
pFN21AB6172
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HindIII HphI KasI KpnI
KspI MamI MluI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI Sse8387I SspBI StuI SwaI
TaqI Tsp45I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 1461 GACGTC GACGT^C
AccIII 1340 TCCGGA T^CCGGA
AcyI 1461 GACGTC G[GA]^CG[CT]C
AlwI 919 GATCC GGATC(4/5)
AlwNI 1517 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1742 GTGCTC G[AT]GC[AT]^C
AspI 184 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 592 GGACC G^G[AT]CC
BalI 535 TGGCCA TGG^CCA
BciVI 267 GTATCC GTATCC(6/5)
BcnI 376 CCGGG CC^[GC]GG
BmyI 1742 GTGCTC G[AGT]GC[ACT]^C
BsaAI 102 TACGTA [CT]AC^GT[GA]
BsaHI 1461 GACGTC G[GA]^CG[CT]C
BsaWI 1340 TCCGGA [AT]^CCGG[AT]
BseAI 1340 TCCGGA T^CCGGA
BsiHKAI 1742 GTGCTC G[AT]GC[AT]^C
BsiYI 210 CCTGATGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 210 CCTGATGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 1742 GTGCTC G[AGT]GC[ACT]^C
BspEI 1340 TCCGGA T^CCGGA
BspHI 1124 TCATGA T^CATGA
BspLU11I 1666 ACATGT A^CATGT
BsrDI 493 GCAATG GCAATG(2/0)
BssSI 152 CTCGTG CACGAG(-5/-1)
BstF5I 990 CATCC GGATG(2/0)
CfoI 47 GCGC GCG^C
DraI 775 TTTAAA TTT^AAA
DraII 591 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 535 TGGCCA [CT]^GGCC[GA]
EarI 1285 GAAGAG CTCTTC(1/4)
EcoO109I 591 AGGACCT [GA]G^G[ACGT]CC[CT]
FokI 990 CATCC GGATG(9/13)
HaeII 46 AGCGCC [GA]GCGC^[CT]
HhaI 47 GCGC GCG^C
Hin1I 1461 GACGTC G[GA]^CG[CT]C
HinP1I 47 GCGC G^CGC
HincII 304 GTTAAC GT[CT]^[GA]AC
HindII 304 GTTAAC GT[CT]^[GA]AC
HpaI 304 GTTAAC GTT^AAC
Ksp632I 1285 GAAGAG CTCTTC(1/4)
MluNI 535 TGGCCA TGG^CCA
MroI 1340 TCCGGA T^CCGGA
MscI 535 TGGCCA TGG^CCA
MslI 171 CACTGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1030 CAGCTG C[AC]G^C[GT]G
MwoI 610 GCTTGCTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 376 CCGGG CC^[GC]GG
NcoI 922 CCATGG C^CATGG
PflFI 184 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 591 AGGACCT [GA]G^G[AT]CC[CT]
PstI 276 CTGCAG CTGCA^G
PvuII 1030 CAGCTG CAG^CTG
RcaI 1124 TCATGA T^CATGA
ScaI 959 AGTACT AGT^ACT
SfcI 276 CTGCAG C^T[GA][CT]AG
SnaBI 102 TACGTA TAC^GTA
SpeI 1233 ACTAGT A^CTAGT
SphI 752 GCATGC GCATG^C
SspI 1510 AATATT AAT^ATT
Tth111I 184 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1086 CCATTTTGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 99 GTCTAC GT^[AC][GT]AC
1400 GTCTAC
AflIII 53 ACACGT A^C[GA][CT]GT
1666 ACATGT
BbsI 1102 GAAGAC GAAGAC(2/6)
1452 GAAGAC
BglII 561 AGATCT A^GATCT
1620 AGATCT
BpuAI 1102 GAAGAC GAAGAC(2/6)
1452 GAAGAC
BseRI 233 GAGGAG GAGGAG(10/8)
1288 GAGGAG
BstYI 561 AGATCT [GA]^GATC[CT]
1620 AGATCT
Cfr13I 592 GGACC G^G[ACGT]CC
794 GGCCC
DrdI 543 GACTTTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1455 GACACAGACGTC
EcoT14I 922 CCATGG C^C[AT][AT]GG
949 CCAAGG
FauI 770 CCCGC CCCGC(4/6)
835 GCGGG
MfeI 639 CAATTG C^AATTG
1639 CAATTG
MflI 561 AGATCT [GA]^GATC[CT]
1620 AGATCT
MunI 639 CAATTG C^AATTG
1639 CAATTG
NlaIV 126 GGTTCC GG[ACGT]^[ACGT]CC
645 GGAACC
Sau96I 592 GGACC G^G[ACGT]CC
794 GGCCC
SfaNI 213 GATGC GCATC(5/9)
1117 GATGC
StyI 922 CCATGG C^C[AT][AT]GG
949 CCAAGG
XhoII 561 AGATCT [GA]^GATC[CT]
1620 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AseI AsnI BbvI BfaI BpmI
BsaJI BsmAI BsrI BssKI BstNI
Cac8I DdeI DpnII DsaI EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MspI MvaI
NdeII NlaIII NspI PleI PshBI
RsaI Sau3AI ScrFI TfiI Tru9I
TseI Tsp509I TspRI