(Circular) (Six-base) MAPSORT of: pFN21AB6172.seq Check: 8103 from: 1 to: 6517 pFN21AB6172 6517 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3470 4327 278 Size: 53 83 186 936 1934 857 2468 Fragments arranged by size: 2468 1934 936 857 186 83 53 AccI GT'mk_AC Cuts at: 1937 2105 3406 3860 1937 Size: 168 1301 454 4594 Fragments arranged by size: 4594 1301 454 168 Acc65I G'GTAC_C Cuts at: 3838 3838 Size: 6517 AclI AA'CG_TT Cuts at: 1563 4644 5017 1563 Size: 3081 373 3063 Fragments arranged by size: 3081 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2334 3576 4580 5964 6232 1632 Size: 702 1242 1004 1384 268 1917 Fragments arranged by size: 1917 1384 1242 1004 702 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6320 AflIII A'CryG_T Cuts at: 1203 2058 3671 5416 6393 1203 Size: 855 1613 1745 977 1327 Fragments arranged by size: 1745 1613 1327 977 855 AgeI A'CCGG_T Cuts at: 5348 5348 Size: 6517 AhdI GACnn_n'nnGTC Cuts at: 5246 5246 Size: 6517 AleI CACnn'nnGTG Cuts at: 1166 2180 1166 Size: 1014 5503 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2784 1184 Size: 1600 4917 AlwNI CAG_nnn'CTG Cuts at: 3527 4260 5832 3527 Size: 733 1572 4212 Fragments arranged by size: 4212 1572 733 ApaLI G'TGCA_C Cuts at: 4574 5730 4574 Size: 1156 5361 ApoI r'AATT_y Cuts at: 1493 2038 2302 2359 3826 4052 4204 6313 1493 Size: 545 264 57 1467 226 152 2109 1697 Fragments arranged by size: 2109 1697 1467 545 264 226 152 57 AseI AT'TA_AT Cuts at: 160 1032 3150 3341 3426 5071 160 Size: 872 2118 191 85 1645 1606 Fragments arranged by size: 2118 1645 1606 872 191 85 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 AvaI C'yCGr_G Cuts at: 1870 1943 3842 1870 Size: 73 1899 4545 Fragments arranged by size: 4545 1899 73 BamHI G'GATC_C Cuts at: 3847 4214 3847 Size: 367 6150 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3838 5293 618 Size: 325 217 627 2051 1455 1842 Fragments arranged by size: 2051 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3836 1391 Size: 2445 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3114 3464 928 Size: 971 1215 350 3981 Fragments arranged by size: 3981 1215 971 350 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4708 4742 1903 Size: 34 2771 34 3678 Fragments arranged by size: 3678 2771 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2283 4409 5619 2283 Size: 2126 1210 3181 Fragments arranged by size: 3181 2126 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6517 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5128 136 Size: 107 122 71 631 605 3456 1525 Fragments arranged by size: 3456 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2566 3625 6512 2566 Size: 1059 2887 2571 Fragments arranged by size: 2887 2571 1059 BlpI GC'TnA_GC Cuts at: 3934 3934 Size: 6517 Bme1580I G_kGCm'C Cuts at: 1790 4578 5734 1790 Size: 2788 1156 2573 Fragments arranged by size: 2788 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6517 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4248 5206 442 Size: 416 53 83 399 24 2831 958 1753 Fragments arranged by size: 2831 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6517 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2172 2172 Size: 6517 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2431 2622 2937 3123 3243 5177 1239 Size: 1192 191 315 186 120 1934 2579 Fragments arranged by size: 2579 1934 1192 315 191 186 120 Bpu10I CC'TnA_GC Cuts at: 2823 2823 Size: 6517 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4642 5507 5805 4642 Size: 865 298 5354 Fragments arranged by size: 5354 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5180 882 Size: 617 3681 2219 Fragments arranged by size: 3681 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2109 4308 493 Size: 711 905 2199 2702 Fragments arranged by size: 2702 2199 905 711 BsaBI GATnn'nnATC Cuts at: 6511 6511 Size: 6517 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3467 4324 4706 5341 Size: 53 83 186 936 1934 857 382 635 Cuts at: 5341 275 Size: 1451 Fragments arranged by size: 1934 1451 936 857 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3345 4950 5348 5622 5769 6180 3345 Size: 1605 398 274 147 411 3682 Fragments arranged by size: 3682 1605 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2595 2625 1178 Size: 30 165 30 1192 30 5070 Fragments arranged by size: 5070 1192 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2253 3308 1186 Size: 1067 1055 4395 Fragments arranged by size: 4395 1067 1055 BseYI C'CCAG_C Cuts at: 2126 5720 6216 2126 Size: 3594 496 2427 Fragments arranged by size: 3594 2427 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5944 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3967 4728 5756 1540 Size: 275 186 1966 761 1028 2301 Fragments arranged by size: 2301 1966 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3751 4578 4663 5734 3751 Size: 827 85 1071 4534 Fragments arranged by size: 4534 1071 827 85 BsmI GAATG_Cn' Cuts at: 4028 4121 4028 Size: 93 6424 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3751 3836 4578 4663 5734 1391 Size: 399 1961 85 742 85 1071 2174 Fragments arranged by size: 2174 1961 1071 742 399 85 85 BspEI T'CCGG_A Cuts at: 3345 6180 3345 Size: 2835 3682 BspHI T'CATG_A Cuts at: 3129 4406 6136 3129 Size: 1277 1730 3510 Fragments arranged by size: 3510 1730 1277 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3872 844 Size: 3028 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4404 1394 Size: 429 2581 3507 Fragments arranged by size: 3507 2581 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2505 5012 5186 65 Size: 1183 1257 2507 174 1396 Fragments arranged by size: 2507 1396 1257 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5161 5348 1700 Size: 110 3351 187 2869 Fragments arranged by size: 3351 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6517 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6517 BssSI C'ACGA_G Cuts at: 2157 4577 5589 2157 Size: 2420 1012 3085 Fragments arranged by size: 3085 2420 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6517 BstBI TT'CG_AA Cuts at: 5321 5321 Size: 6517 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6517 BstYI r'GATC_y Cuts at: 1972 2566 3625 3847 4214 4599 4616 6068 6154 Size: 594 1059 222 367 385 17 1452 86 Cuts at: 6154 6166 6512 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1059 594 385 367 346 222 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2407 2927 3159 513 Size: 552 1342 520 232 3871 Fragments arranged by size: 3871 1342 552 520 232 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5778 Fragments arranged by size: 5778 683 56 BtsI GCAGTG_nn' Cuts at: 4033 4827 4847 4033 Size: 794 20 5703 Fragments arranged by size: 5703 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6517 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6482 DraI TTT'AAA Cuts at: 2782 3821 2782 Size: 1039 5478 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2554 3466 5524 809 Size: 507 1238 912 2058 1802 Fragments arranged by size: 2058 1802 1238 912 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2540 3964 4853 Size: 54 1425 50 275 18 710 1424 889 Cuts at: 4853 6482 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1424 889 710 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3964 1537 Size: 275 2152 4090 Fragments arranged by size: 4090 2152 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3285 4447 1353 Size: 1932 1162 3423 Fragments arranged by size: 3423 1932 1162 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5104 5478 5624 387 Size: 1064 138 3515 374 146 1280 Fragments arranged by size: 3515 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2334 2431 2622 2937 3123 3243 3576 Size: 393 702 97 191 315 186 120 333 Cuts at: 3576 4580 5177 5964 6232 1239 Size: 1004 597 787 268 1524 Fragments arranged by size: 1524 1004 787 702 597 393 333 315 268 191 186 120 97 EcoO109I rG'GnC_Cy Cuts at: 2597 2597 Size: 6517 EcoRI G'AATT_C Cuts at: 3826 4204 3826 Size: 378 6139 FspI TGC'GCA Cuts at: 5023 5023 Size: 6517 HaeII r_GCGC'y Cuts at: 1678 2055 5664 1678 Size: 377 3609 2531 Fragments arranged by size: 3609 2531 377 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3124 3156 5158 5190 5232 Size: 32 135 32 1552 32 2002 32 42 Cuts at: 5232 5264 1373 Size: 32 2626 Fragments arranged by size: 2626 2002 1552 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2311 3861 4107 669 Size: 988 281 373 1550 246 3079 Fragments arranged by size: 3079 1550 988 373 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6517 HpaI GTT'AAC Cuts at: 2311 4107 2311 Size: 1796 4721 KpnI G_GTAC'C Cuts at: 3842 3842 Size: 6517 MfeI C'AATT_G Cuts at: 2644 3644 4116 2644 Size: 1000 472 5045 Fragments arranged by size: 5045 1000 472 MluI A'CGCG_T Cuts at: 6393 6393 Size: 6517 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5631 5815 1305 Size: 4326 184 2007 Fragments arranged by size: 4326 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2542 10 Size: 54 1425 343 710 3985 Fragments arranged by size: 3985 1425 710 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2180 4475 4834 4993 518 Size: 648 1014 2295 359 159 2042 Fragments arranged by size: 2295 2042 1014 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3037 3933 4610 5758 6003 1606 Size: 1431 896 677 1148 245 2120 Fragments arranged by size: 2120 1431 1148 896 677 245 NcoI C'CATG_G Cuts at: 513 1065 2927 513 Size: 552 1862 4103 Fragments arranged by size: 4103 1862 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6517 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6517 NotI GC'GGCC_GC Cuts at: 3964 3964 Size: 6517 NspI r_CATG'y Cuts at: 1132 1229 2761 2771 2942 3675 3875 5420 1132 Size: 97 1532 10 171 733 200 1545 2229 Fragments arranged by size: 2229 1545 1532 733 200 171 97 10 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6517 PciI A'CATG_T Cuts at: 3671 5416 3671 Size: 1745 4772 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6517 PfoI T'CCnGG_A Cuts at: 3396 3396 Size: 6517 PmeI GTTT'AAAC Cuts at: 3821 3821 Size: 6517 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4586 4618 6099 6131 4586 Size: 32 1481 32 4972 Fragments arranged by size: 4972 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2597 2597 Size: 6517 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6517 PsiI TTA'TAA Cuts at: 4087 4087 Size: 6517 PstI C_TGCA'G Cuts at: 830 2285 3869 830 Size: 1455 1584 3478 Fragments arranged by size: 3478 1584 1455 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6517 PvuII CAG'CTG Cuts at: 3037 3037 Size: 6517 SalI G'TCGA_C Cuts at: 1936 3859 1936 Size: 1923 4594 SbfI CC_TGCA'GG Cuts at: 3869 3869 Size: 6517 ScaI AGT'ACT Cuts at: 2966 4765 6477 2966 Size: 1799 1712 3006 Fragments arranged by size: 3006 1799 1712 SfcI C'TryA_G Cuts at: 826 1045 2281 3865 5000 5681 5872 826 Size: 219 1236 1584 1135 681 191 1471 Fragments arranged by size: 1584 1471 1236 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6517 SmaI CCC'GGG Cuts at: 1872 3844 1872 Size: 1972 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4621 5522 5784 820 Size: 197 926 2678 901 262 1553 Fragments arranged by size: 2678 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2109 4308 493 Size: 1616 2199 2702 Fragments arranged by size: 2702 2199 1616 SpeI A'CTAG_T Cuts at: 152 3238 152 Size: 3086 3431 SphI G_CATG'C Cuts at: 1132 2761 3875 1132 Size: 1629 1114 3774 Fragments arranged by size: 3774 1629 1114 SspI AAT'ATT Cuts at: 5 52 3517 4441 5 Size: 47 3465 924 2081 Fragments arranged by size: 3465 2081 924 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6517 StyI C'CwwG_G Cuts at: 513 1065 2927 2954 3956 513 Size: 552 1862 27 1002 3074 Fragments arranged by size: 3074 1862 1002 552 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4544 4561 4714 4899 5344 325 Size: 1255 2964 17 153 185 445 1498 Fragments arranged by size: 2964 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2964 3782 4763 Size: 274 80 33 51 1443 987 818 981 Cuts at: 4763 6475 96 Size: 1712 138 Fragments arranged by size: 1712 1443 987 981 818 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2595 2627 6477 6509 2595 Size: 32 3850 32 2603 Fragments arranged by size: 3850 2603 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2192 1316 Size: 876 5641 XbaI T'CTAG_A Cuts at: 3853 3853 Size: 6517 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3098 3098 Size: 6517 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6517 XmaI C'CCGG_G Cuts at: 1870 3842 1870 Size: 1972 4545 XmnI GAAnn'nnTTC Cuts at: 4646 4646 Size: 6517 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3468 4325 276 Size: 53 83 186 936 1934 857 2468 Fragments arranged by size: 2468 1934 936 857 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI