Restriction map for
pFN21AB6172
(Linear) (Six-base) MAPSORT of: KIBB6172.seq Check: 6323 from: 1 to: 1812
KIBB6172.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AatII G_ACGT'C
Cuts at: 0 1465 1812
Size: 1465 347
AccI GT'mk_AC
Cuts at: 0 100 1401 1812
Size: 100 1301 411
Fragments arranged by size:
1301 411 100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1571 1812
Size: 329 1242 241
Fragments arranged by size:
1242 329 241
AflIII A'CryG_T
Cuts at: 0 53 1666 1812
Size: 53 1613 146
Fragments arranged by size:
1613 146 53
AleI CACnn'nnGTG
Cuts at: 0 175 1812
Size: 175 1637
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 779 1812
Size: 779 1033
AlwNI CAG_nnn'CTG
Cuts at: 0 1522 1812
Size: 1522 290
ApoI r'AATT_y
Cuts at: 0 33 297 354 1812
Size: 33 264 57 1458
Fragments arranged by size:
1458 264 57 33
AseI AT'TA_AT
Cuts at: 0 1145 1336 1421 1812
Size: 1145 191 85 391
Fragments arranged by size:
1145 391 191 85
BbsI GAAGACnn'nnnn_
Cuts at: 0 1109 1459 1812
Size: 1109 350 353
Fragments arranged by size:
1109 353 350
BciVI GTATCCnnnnn_n'
Cuts at: 0 278 1812
Size: 278 1534
BglII A'GATC_T
Cuts at: 0 561 1620 1812
Size: 561 1059 192
Fragments arranged by size:
1059 561 192
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 167 1812
Size: 167 1645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 617 932 1118 1238 1812
Size: 426 191 315 186 120 574
Fragments arranged by size:
574 426 315 191 186 120
Bpu10I CC'TnA_GC
Cuts at: 0 818 1812
Size: 818 994
BsaAI yAC'GTr
Cuts at: 0 104 1812
Size: 104 1708
BsaHI Gr'CG_yC
Cuts at: 0 1462 1812
Size: 1462 350
BsaWI w'CCGG_w
Cuts at: 0 1340 1812
Size: 1340 472
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 590 620 1812
Size: 590 30 1192
Fragments arranged by size:
1192 590 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 248 1303 1812
Size: 248 1055 509
Fragments arranged by size:
1055 509 248
BseYI C'CCAG_C
Cuts at: 0 121 1812
Size: 121 1691
BsiHKAI G_wGCw'C
Cuts at: 0 1746 1812
Size: 1746 66
Bsp1286I G_dGCh'C
Cuts at: 0 1746 1812
Size: 1746 66
BspEI T'CCGG_A
Cuts at: 0 1340 1812
Size: 1340 472
BspHI T'CATG_A
Cuts at: 0 1124 1812
Size: 1124 688
BsrDI GCAATG_nn'
Cuts at: 0 500 1812
Size: 500 1312
BssSI C'ACGA_G
Cuts at: 0 152 1812
Size: 152 1660
BstYI r'GATC_y
Cuts at: 0 561 1620 1812
Size: 561 1059 192
Fragments arranged by size:
1059 561 192
BtgI C'CryG_G
Cuts at: 0 402 922 1154 1812
Size: 402 520 232 658
Fragments arranged by size:
658 520 402 232
DraI TTT'AAA
Cuts at: 0 777 1812
Size: 777 1035
DrdI GACnn_nn'nnGTC
Cuts at: 0 549 1461 1812
Size: 549 912 351
Fragments arranged by size:
912 549 351
EaeI y'GGCC_r
Cuts at: 0 535 1812
Size: 535 1277
EarI CTCTTCn'nnn_
Cuts at: 0 1280 1812
Size: 1280 532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 426 617 932 1118 1238 1571 1812
Size: 329 97 191 315 186 120 333 241
Fragments arranged by size:
333 329 315 241 191 186 120 97
EcoO109I rG'GnC_Cy
Cuts at: 0 592 1812
Size: 592 1220
HaeII r_GCGC'y
Cuts at: 0 50 1812
Size: 50 1762
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1119 1151 1812
Size: 1119 32 661
Fragments arranged by size:
1119 661 32
HincII GTy'rAC
Cuts at: 0 306 1812
Size: 306 1506
HpaI GTT'AAC
Cuts at: 0 306 1812
Size: 306 1506
MfeI C'AATT_G
Cuts at: 0 639 1639 1812
Size: 639 1000 173
Fragments arranged by size:
1000 639 173
MscI TGG'CCA
Cuts at: 0 537 1812
Size: 537 1275
MslI CAynn'nnrTG
Cuts at: 0 175 1812
Size: 175 1637
MspA1I CmG'CkG
Cuts at: 0 1032 1812
Size: 1032 780
NcoI C'CATG_G
Cuts at: 0 922 1812
Size: 922 890
NspI r_CATG'y
Cuts at: 0 756 766 937 1670 1812
Size: 756 10 171 733 142
Fragments arranged by size:
756 733 171 142 10
PciI A'CATG_T
Cuts at: 0 1666 1812
Size: 1666 146
PfoI T'CCnGG_A
Cuts at: 0 1391 1812
Size: 1391 421
PpuMI rG'GwC_Cy
Cuts at: 0 592 1812
Size: 592 1220
PstI C_TGCA'G
Cuts at: 0 280 1812
Size: 280 1532
PvuII CAG'CTG
Cuts at: 0 1032 1812
Size: 1032 780
ScaI AGT'ACT
Cuts at: 0 961 1812
Size: 961 851
SfcI C'TryA_G
Cuts at: 0 276 1812
Size: 276 1536
SnaBI TAC'GTA
Cuts at: 0 104 1812
Size: 104 1708
SpeI A'CTAG_T
Cuts at: 0 1233 1812
Size: 1233 579
SphI G_CATG'C
Cuts at: 0 756 1812
Size: 756 1056
SspI AAT'ATT
Cuts at: 0 1512 1812
Size: 1512 300
StyI C'CwwG_G
Cuts at: 0 922 949 1812
Size: 922 27 863
Fragments arranged by size:
922 863 27
TatI w'GTAC_w
Cuts at: 0 959 1777 1812
Size: 959 818 35
Fragments arranged by size:
959 818 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 590 622 1812
Size: 590 32 1190
Fragments arranged by size:
1190 590 32
Tth111I GACn'n_nGTC
Cuts at: 0 187 1812
Size: 187 1625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1093 1812
Size: 1093 719
ZraI GAC'GTC
Cuts at: 0 1463 1812
Size: 1463 349
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlfI AlwNI ApoI
AseI BbsI BciVI BglII BplI BpmI Bpu10I BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspEI
BspHI BsrDI BssSI BstYI BtgI DraI DrdI EaeI
EarI Eco57MI EcoO109I HaeII Hin4I HincII HpaI MfeI
MscI MslI MspA1I NcoI NspI PciI PfoI PpuMI
PstI PvuII ScaI SfcI SnaBI SpeI SphI SspI
StyI TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbvCI BcgI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BpuEI BsaI BsaBI
BsgI BsiEI BsiWI BsmI BsmBI BspMI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraIII EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
KasI KpnI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SrfI StuI
SwaI TaqII XbaI XhoI XmaI XmnI