(Linear) (Six-base) MAPSORT of: KIEE9555.seq Check: 9940 from: 1 to: 3540 KIEE9555.orf With 182 enzymes: SgfI * December 27, 2011 16:42 .. AccI GT'mk_AC Cuts at: 0 2186 3540 Size: 2186 1354 AclI AA'CG_TT Cuts at: 0 1448 3540 Size: 1448 2092 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 341 563 802 1045 1921 3393 3540 Size: 341 222 239 243 876 1472 147 Fragments arranged by size: 1472 876 341 243 239 222 147 AfeI AGC'GCT Cuts at: 0 2814 3540 Size: 2814 726 AflII C'TTAA_G Cuts at: 0 2625 3540 Size: 2625 915 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2470 3540 Size: 2470 1070 AlwNI CAG_nnn'CTG Cuts at: 0 2688 2700 3255 3540 Size: 2688 12 555 285 Fragments arranged by size: 2688 555 285 12 ApaI G_GGCC'C Cuts at: 0 2658 2873 3540 Size: 2658 215 667 Fragments arranged by size: 2658 667 215 ApaLI G'TGCA_C Cuts at: 0 56 649 3540 Size: 56 593 2891 Fragments arranged by size: 2891 593 56 ApoI r'AATT_y Cuts at: 0 477 892 1069 1407 3540 Size: 477 415 177 338 2133 Fragments arranged by size: 2133 477 415 338 177 AvaI C'yCGr_G Cuts at: 0 812 2872 3323 3346 3426 3540 Size: 812 2060 451 23 80 114 Fragments arranged by size: 2060 812 451 114 80 23 AvrII C'CTAG_G Cuts at: 0 2211 2439 3540 Size: 2211 228 1101 Fragments arranged by size: 2211 1101 228 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 623 656 1808 1841 1875 1908 3540 Size: 623 33 1152 33 34 33 1632 Fragments arranged by size: 1632 1152 623 34 33 33 33 BamHI G'GATC_C Cuts at: 0 2012 3540 Size: 2012 1528 BanI G'GyrC_C Cuts at: 0 872 2854 2937 3172 3540 Size: 872 1982 83 235 368 Fragments arranged by size: 1982 872 368 235 83 BanII G_rGCy'C Cuts at: 0 117 1232 2611 2658 2873 2995 3070 3128 Size: 117 1115 1379 47 215 122 75 58 Cuts at: 3128 3334 3529 3540 Size: 206 195 11 Fragments arranged by size: 1379 1115 215 206 195 122 117 75 58 47 11 BbeI G_GCGC'C Cuts at: 0 876 2858 2941 3176 3540 Size: 876 1982 83 235 364 Fragments arranged by size: 1982 876 364 235 83 BbsI GAAGACnn'nnnn_ Cuts at: 0 1791 3540 Size: 1791 1749 BbvCI CC'TCA_GC Cuts at: 0 2921 3540 Size: 2921 619 BciVI GTATCCnnnnn_n' Cuts at: 0 2227 3484 3540 Size: 2227 1257 56 Fragments arranged by size: 2227 1257 56 BclI T'GATC_A Cuts at: 0 1199 3540 Size: 1199 2341 BfrBI ATG'CAT Cuts at: 0 2105 2160 2535 3540 Size: 2105 55 375 1005 Fragments arranged by size: 2105 1005 375 55 BglI GCCn_nnn'nGGC Cuts at: 0 2434 2946 3540 Size: 2434 512 594 Fragments arranged by size: 2434 594 512 BglII A'GATC_T Cuts at: 0 1522 2036 3540 Size: 1522 514 1504 Fragments arranged by size: 1522 1504 514 BlpI GC'TnA_GC Cuts at: 0 587 2977 3540 Size: 587 2390 563 Fragments arranged by size: 2390 587 563 Bme1580I G_kGCm'C Cuts at: 0 60 653 2076 2658 2873 3540 Size: 60 593 1423 582 215 667 Fragments arranged by size: 1423 667 593 582 215 60 BmrI ACTGGGnnnn_n' Cuts at: 0 280 1349 1669 1699 3540 Size: 280 1069 320 30 1841 Fragments arranged by size: 1841 1069 320 280 30 BmtI G_CTAG'C Cuts at: 0 2359 3540 Size: 2359 1181 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 364 3147 3540 Size: 364 2783 393 Fragments arranged by size: 2783 393 364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 435 469 1370 3394 3540 Size: 435 34 901 2024 146 Fragments arranged by size: 2024 901 435 146 34 Bpu10I CC'TnA_GC Cuts at: 0 2921 3540 Size: 2921 619 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1821 3540 Size: 1821 1719 BsaI GGTCTCn'nnnn_ Cuts at: 0 1960 3540 Size: 1960 1580 BsaAI yAC'GTr Cuts at: 0 2651 3540 Size: 2651 889 BsaBI GATnn'nnATC Cuts at: 0 721 947 1019 3540 Size: 721 226 72 2521 Fragments arranged by size: 2521 721 226 72 BsaHI Gr'CG_yC Cuts at: 0 873 2855 2885 2938 3167 3173 3540 Size: 873 1982 30 53 229 6 367 Fragments arranged by size: 1982 873 367 229 53 30 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1606 1636 3540 Size: 1606 30 1904 Fragments arranged by size: 1904 1606 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 87 387 390 2601 2938 3048 3391 3519 Size: 87 300 3 2211 337 110 343 128 Cuts at: 3519 3540 Size: 21 Fragments arranged by size: 2211 343 337 300 128 110 87 21 3 BseYI C'CCAG_C Cuts at: 0 917 1962 2075 2815 3157 3310 3540 Size: 917 1045 113 740 342 153 230 Fragments arranged by size: 1045 917 740 342 230 153 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1551 2244 3013 3540 Size: 1551 693 769 527 Fragments arranged by size: 1551 769 693 527 BsiEI CG_ry'CG Cuts at: 0 2744 2950 3341 3359 3540 Size: 2744 206 391 18 181 Fragments arranged by size: 2744 391 206 181 18 BsiHKAI G_wGCw'C Cuts at: 0 60 117 653 2770 2930 3128 3436 3529 Size: 60 57 536 2117 160 198 308 93 Cuts at: 3529 3540 Size: 11 Fragments arranged by size: 2117 536 308 198 160 93 60 57 11 BsmI GAATG_Cn' Cuts at: 0 194 300 2174 3540 Size: 194 106 1874 1366 Fragments arranged by size: 1874 1366 194 106 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3488 3540 Size: 3488 52 Bsp1286I G_dGCh'C Cuts at: 0 60 117 653 1232 2076 2611 2658 2770 Size: 60 57 536 579 844 535 47 112 Cuts at: 2770 2873 2930 2995 3070 3128 3334 3436 3529 Size: 103 57 65 75 58 206 102 93 Cuts at: 3529 3540 Size: 11 Fragments arranged by size: 844 579 536 535 206 112 103 102 93 75 65 60 58 57 57 47 11 BspHI T'CATG_A Cuts at: 0 156 563 2060 3540 Size: 156 407 1497 1480 Fragments arranged by size: 1497 1480 407 156 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2350 3540 Size: 2350 1190 BsrBI CCG'CTC Cuts at: 0 413 3180 3540 Size: 413 2767 360 Fragments arranged by size: 2767 413 360 BsrDI GCAATG_nn' Cuts at: 0 1739 1904 3540 Size: 1739 165 1636 Fragments arranged by size: 1739 1636 165 BsrFI r'CCGG_y Cuts at: 0 2857 2887 3258 3340 3540 Size: 2857 30 371 82 200 Fragments arranged by size: 2857 371 200 82 30 BsrGI T'GTAC_A Cuts at: 0 1600 2333 2465 3540 Size: 1600 733 132 1075 Fragments arranged by size: 1600 1075 733 132 BssHII G'CGCG_C Cuts at: 0 3046 3281 3540 Size: 3046 235 259 Fragments arranged by size: 3046 259 235 BstAPI GCAn_nnn'nTGC Cuts at: 0 1652 3540 Size: 1652 1888 BstEII G'GTnAC_C Cuts at: 0 1726 3096 3540 Size: 1726 1370 444 Fragments arranged by size: 1726 1370 444 BstYI r'GATC_y Cuts at: 0 531 1522 2012 2036 2572 3209 3540 Size: 531 991 490 24 536 637 331 Fragments arranged by size: 991 637 536 531 490 331 24 BtgI C'CryG_G Cuts at: 0 48 602 2522 2778 2831 3149 3290 3335 Size: 48 554 1920 256 53 318 141 45 Cuts at: 3335 3540 Size: 205 Fragments arranged by size: 1920 554 318 256 205 141 53 48 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 708 3540 Size: 708 2832 BtsI GCAGTG_nn' Cuts at: 0 915 1539 3096 3540 Size: 915 624 1557 444 Fragments arranged by size: 1557 915 624 444 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1767 1802 3540 Size: 1767 35 1738 Fragments arranged by size: 1767 1738 35 DraIII CAC_nnn'GTG Cuts at: 0 2405 3540 Size: 2405 1135 EaeI y'GGCC_r Cuts at: 0 863 1033 1775 2477 2741 2912 2947 2996 Size: 863 170 742 702 264 171 35 49 Cuts at: 2996 3110 3260 3338 3356 3540 Size: 114 150 78 18 184 Fragments arranged by size: 863 742 702 264 184 171 170 150 114 78 49 35 18 EagI C'GGCC_G Cuts at: 0 2741 2947 3338 3356 3540 Size: 2741 206 391 18 184 Fragments arranged by size: 2741 391 206 184 18 EarI CTCTTCn'nnn_ Cuts at: 0 356 408 613 2593 2662 3540 Size: 356 52 205 1980 69 878 Fragments arranged by size: 1980 878 356 205 69 52 EcoICRI GAG'CTC Cuts at: 0 115 3126 3527 3540 Size: 115 3011 401 13 Fragments arranged by size: 3011 401 115 13 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 341 435 469 563 802 1045 1370 1921 Size: 341 94 34 94 239 243 325 551 Cuts at: 1921 3393 3394 3540 Size: 1472 1 146 Fragments arranged by size: 1472 551 341 325 243 239 146 94 94 34 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 1839 3540 Size: 1839 1701 EcoRV GAT'ATC Cuts at: 0 949 3540 Size: 949 2591 FspI TGC'GCA Cuts at: 0 2500 2530 2850 3540 Size: 2500 30 320 690 Fragments arranged by size: 2500 690 320 30 FspAI rTGC'GCAy Cuts at: 0 2530 3540 Size: 2530 1010 HaeII r_GCGC'y Cuts at: 0 876 2507 2700 2792 2798 2816 2858 2902 Size: 876 1631 193 92 6 18 42 44 Cuts at: 2902 2941 3176 3540 Size: 39 235 364 Fragments arranged by size: 1631 876 364 235 193 92 44 42 39 18 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 382 414 1184 1216 1343 1375 2536 2568 Size: 382 32 770 32 127 32 1161 32 Cuts at: 2568 3007 3039 3540 Size: 439 32 501 Fragments arranged by size: 1161 770 501 439 382 127 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2589 3540 Size: 2589 951 KasI G'GCGC_C Cuts at: 0 872 2854 2937 3172 3540 Size: 872 1982 83 235 368 Fragments arranged by size: 1982 872 368 235 83 MfeI C'AATT_G Cuts at: 0 1292 3540 Size: 1292 2248 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1431 1573 3540 Size: 1431 142 1967 Fragments arranged by size: 1967 1431 142 MscI TGG'CCA Cuts at: 0 865 1777 2479 3540 Size: 865 912 702 1061 Fragments arranged by size: 1061 912 865 702 MslI CAynn'nnrTG Cuts at: 0 844 1243 2401 2452 3226 3540 Size: 844 399 1158 51 774 314 Fragments arranged by size: 1158 844 774 399 314 51 MspA1I CmG'CkG Cuts at: 0 1586 1747 3151 3292 3337 3540 Size: 1586 161 1404 141 45 203 Fragments arranged by size: 1586 1404 203 161 141 45 NaeI GCC'GGC Cuts at: 0 2859 2889 3260 3540 Size: 2859 30 371 280 Fragments arranged by size: 2859 371 280 30 NarI GG'CG_CC Cuts at: 0 873 2855 2938 3173 3540 Size: 873 1982 83 235 367 Fragments arranged by size: 1982 873 367 235 83 NcoI C'CATG_G Cuts at: 0 602 3540 Size: 602 2938 NdeI CA'TA_TG Cuts at: 0 2101 3540 Size: 2101 1439 NgoMIV G'CCGG_C Cuts at: 0 2857 2887 3258 3540 Size: 2857 30 371 282 Fragments arranged by size: 2857 371 282 30 NheI G'CTAG_C Cuts at: 0 2355 3540 Size: 2355 1185 NotI GC'GGCC_GC Cuts at: 0 2947 3356 3540 Size: 2947 409 184 Fragments arranged by size: 2947 409 184 NsiI A_TGCA'T Cuts at: 0 2107 2162 2537 3540 Size: 2107 55 375 1003 Fragments arranged by size: 2107 1003 375 55 NspI r_CATG'y Cuts at: 0 93 1242 1646 1655 2451 2535 2539 3540 Size: 93 1149 404 9 796 84 4 1001 Fragments arranged by size: 1149 1001 796 404 93 84 9 4 PflMI CCAn_nnn'nTGG Cuts at: 0 1297 3540 Size: 1297 2243 PmlI CAC'GTG Cuts at: 0 2651 3540 Size: 2651 889 PsiI TTA'TAA Cuts at: 0 2137 3540 Size: 2137 1403 PspOMI G'GGCC_C Cuts at: 0 2654 2869 3540 Size: 2654 215 671 Fragments arranged by size: 2654 671 215 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1111 1143 3540 Size: 1111 32 2397 Fragments arranged by size: 2397 1111 32 PvuII CAG'CTG Cuts at: 0 1586 1747 3540 Size: 1586 161 1793 Fragments arranged by size: 1793 1586 161 RsrII CG'GwC_CG Cuts at: 0 177 3540 Size: 177 3363 SacI G_AGCT'C Cuts at: 0 117 3128 3529 3540 Size: 117 3011 401 11 Fragments arranged by size: 3011 401 117 11 SacII CC_GC'GG Cuts at: 0 3152 3293 3338 3540 Size: 3152 141 45 202 Fragments arranged by size: 3152 202 141 45 SapI GCTCTTCn'nnn_ Cuts at: 0 2662 3540 Size: 2662 878 SfcI C'TryA_G Cuts at: 0 458 744 1793 3540 Size: 458 286 1049 1747 Fragments arranged by size: 1747 1049 458 286 SfoI GGC'GCC Cuts at: 0 874 2856 2939 3174 3540 Size: 874 1982 83 235 366 Fragments arranged by size: 1982 874 366 235 83 SmaI CCC'GGG Cuts at: 0 814 3325 3540 Size: 814 2511 215 Fragments arranged by size: 2511 814 215 SmlI C'TyrA_G Cuts at: 0 1836 2625 3540 Size: 1836 789 915 Fragments arranged by size: 1836 915 789 SphI G_CATG'C Cuts at: 0 1655 2535 3540 Size: 1655 880 1005 Fragments arranged by size: 1655 1005 880 SspI AAT'ATT Cuts at: 0 559 3540 Size: 559 2981 StyI C'CwwG_G Cuts at: 0 602 806 1057 1764 2211 2439 2894 3540 Size: 602 204 251 707 447 228 455 646 Fragments arranged by size: 707 646 602 455 447 251 228 204 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2403 3540 Size: 2403 1137 TatI w'GTAC_w Cuts at: 0 1600 1637 1707 2333 2465 3540 Size: 1600 37 70 626 132 1075 Fragments arranged by size: 1600 1075 626 132 70 37 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 267 813 873 1215 1746 2520 3369 3540 Size: 267 546 60 342 531 774 849 171 Fragments arranged by size: 849 774 546 531 342 267 171 60 XmaI C'CCGG_G Cuts at: 0 812 3323 3540 Size: 812 2511 217 Fragments arranged by size: 2511 812 217 XmnI GAAnn'nnTTC Cuts at: 0 1106 3540 Size: 1106 2434 Enzymes that do cut: AccI AclI AcuI AfeI AflII AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoRV FspI FspAI HaeII Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PflMI PmlI PsiI PspOMI PsrI PvuII RsrII SacI SacII SapI SfcI SfoI SmaI SmlI SphI SspI StyI TaqII TatI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AflIII AgeI AhdI AleI AloI AscI AseI AsiSI BcgI BmgBI BsaWI BsiWI BspEI BssSI BstBI BstXI BstZ17I Bsu36I ClaI DraI DrdI EciI EcoO109I EcoRI FalI FseI HindIII HpaI KpnI MluI NruI PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI PspXI PstI PvuI SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI TstI Tth111I XbaI XhoI ZraI