Restriction map for
pFN21AE1729
(Circular) (Six-base) MAPSORT of: pFN21AE1729.seq Check: 35 from: 1 to: 5416
pFN21AE1729 5416 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3226 278
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2759 1937
Size: 822 4594
Acc65I G'GTAC_C
Cuts at: 2108 2737 2108
Size: 629 4787
AclI AA'CG_TT
Cuts at: 1563 3543 3916 1563
Size: 1980 373 3063
Fragments arranged by size:
3063 1980 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3479 4863 5131 1632
Size: 1847 1384 268 1917
Fragments arranged by size:
1917 1847 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5219
AflIII A'CryG_T
Cuts at: 1203 4315 5292 1203
Size: 3112 977 1327
Fragments arranged by size:
3112 1327 977
AgeI A'CCGG_T
Cuts at: 4247 4247
Size: 5416
AhdI GACnn_n'nnGTC
Cuts at: 4145 4145
Size: 5416
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5416
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2363 2395 2363
Size: 32 5384
AlwNI CAG_nnn'CTG
Cuts at: 2072 2420 2684 3159 4731 2072
Size: 348 264 475 1572 2757
Fragments arranged by size:
2757 1572 475 348 264
ApaLI G'TGCA_C
Cuts at: 3473 4629 3473
Size: 1156 4260
ApoI r'AATT_y
Cuts at: 1493 2258 2336 2725 2951 3103 5212 1493
Size: 765 78 389 226 152 2109 1697
Fragments arranged by size:
2109 1697 765 389 226 152 78
AseI AT'TA_AT
Cuts at: 160 1032 3970 160
Size: 872 2938 1606
Fragments arranged by size:
2938 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
AvaI C'yCGr_G
Cuts at: 1870 1943 2741 1870
Size: 73 798 4545
Fragments arranged by size:
4545 798 73
BamHI G'GATC_C
Cuts at: 2746 3113 2746
Size: 367 5049
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 2737 4192 618
Size: 325 217 627 321 629 1455 1842
Fragments arranged by size:
1842 1455 629 627 325 321 217
BanII G_rGCy'C
Cuts at: 1391 2052 2735 1391
Size: 661 683 4072
Fragments arranged by size:
4072 683 661
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2310 928
Size: 971 411 4034
Fragments arranged by size:
4034 971 411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3607 3641 1903
Size: 34 1670 34 3678
Fragments arranged by size:
3678 1670 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2659 3308 4518 2659
Size: 649 1210 3557
Fragments arranged by size:
3557 1210 649
BclI T'GATC_A
Cuts at: 1548 2710 1548
Size: 1162 4254
BfrBI ATG'CAT
Cuts at: 2198 2198
Size: 5416
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2035 4027 136
Size: 107 122 71 631 605 363 1992 1525
Fragments arranged by size:
1992 1525 631 605 363 122 107 71
BglII A'GATC_T
Cuts at: 5411 5411
Size: 5416
BlpI GC'TnA_GC
Cuts at: 2833 2833
Size: 5416
Bme1580I G_kGCm'C
Cuts at: 1790 3477 4633 1790
Size: 1687 1156 2573
Fragments arranged by size:
2573 1687 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5416
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3147 4105 442
Size: 416 53 83 399 24 1730 958 1753
Fragments arranged by size:
1753 1730 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2579 4076 1239
Size: 1340 1497 2579
Fragments arranged by size:
2579 1497 1340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3541 4406 4704 3541
Size: 865 298 4253
Fragments arranged by size:
4253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4079 882
Size: 617 2580 2219
Fragments arranged by size:
2580 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3207 493
Size: 711 2003 2702
Fragments arranged by size:
2702 2003 711
BsaBI GATnn'nnATC
Cuts at: 2709 5410 2709
Size: 2701 2715
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3223 3605 4240 275
Size: 53 83 186 936 1690 382 635 1451
Fragments arranged by size:
1690 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3849 4247 4521 4668 5079 3849
Size: 398 274 147 411 4186
Fragments arranged by size:
4186 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2363 2393 1178
Size: 30 165 30 960 30 4201
Fragments arranged by size:
4201 960 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5416
BseYI C'CCAG_C
Cuts at: 2612 4619 5115 2612
Size: 2007 496 2913
Fragments arranged by size:
2913 2007 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4843
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2866 3627 4655 1540
Size: 275 186 865 761 1028 2301
Fragments arranged by size:
2301 1028 865 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3477 3562 4633 3477
Size: 85 1071 4260
Fragments arranged by size:
4260 1071 85
BsmI GAATG_Cn'
Cuts at: 2257 2402 2580 2927 3020 2257
Size: 145 178 347 93 4653
Fragments arranged by size:
4653 347 178 145 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2735 3477 3562 4633 1391
Size: 399 262 683 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 683 399 262 85
BspEI T'CCGG_A
Cuts at: 5079 5079
Size: 5416
BspHI T'CATG_A
Cuts at: 3305 5035 3305
Size: 1730 3686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2771 844
Size: 1927 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3303 1394
Size: 429 1480 3507
Fragments arranged by size:
3507 1480 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2382 2394 3911 4085 65
Size: 1183 1134 12 1517 174 1396
Fragments arranged by size:
1517 1396 1183 1134 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 4060 4247 1700
Size: 110 2250 187 2869
Fragments arranged by size:
2869 2250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5416
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5416
BssSI C'ACGA_G
Cuts at: 3476 4488 3476
Size: 1012 4404
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5416
BstBI TT'CG_AA
Cuts at: 4220 4220
Size: 5416
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5416
BstYI r'GATC_y
Cuts at: 1972 2416 2746 3113 3498 3515 4967 5053 5065
Size: 444 330 367 385 17 1452 86 12
Cuts at: 5065 5411 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 444 385 367 346 330 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2421 2421
Size: 5416
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4864
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2104 487
Size: 683 56 878 3799
Fragments arranged by size:
3799 878 683 56
BtsI GCAGTG_nn'
Cuts at: 2382 2932 3726 3746 2382
Size: 550 794 20 4052
Fragments arranged by size:
4052 794 550 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5381
DraI TTT'AAA
Cuts at: 2720 2720
Size: 5416
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4423 809
Size: 507 3107 1802
Fragments arranged by size:
3107 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2863 3752 5381
Size: 54 1425 50 275 18 1033 889 1629
Cuts at: 5381 8
Size: 43
Fragments arranged by size:
1629 1425 1033 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2863 1537
Size: 275 1051 4090
Fragments arranged by size:
4090 1051 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2292 3346 1353
Size: 939 1054 3423
Fragments arranged by size:
3423 1054 939
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4003 4377 4523 387
Size: 1064 138 2414 374 146 1280
Fragments arranged by size:
2414 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2579 3479 4076 4863 5131 1239
Size: 393 947 900 597 787 268 1524
Fragments arranged by size:
1524 947 900 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2095 2095
Size: 5416
EcoRI G'AATT_C
Cuts at: 2725 3103 2725
Size: 378 5038
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2594 2594
Size: 5416
FspI TGC'GCA
Cuts at: 3922 3922
Size: 5416
HaeII r_GCGC'y
Cuts at: 1678 4563 1678
Size: 2885 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4057 4089 4131 4163 1373
Size: 32 135 32 2485 32 42 32 2626
Fragments arranged by size:
2626 2485 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2760 3006 669
Size: 988 281 822 246 3079
Fragments arranged by size:
3079 988 822 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5416
HpaI GTT'AAC
Cuts at: 3006 3006
Size: 5416
KpnI G_GTAC'C
Cuts at: 2112 2741 2112
Size: 629 4787
MfeI C'AATT_G
Cuts at: 3015 3015
Size: 5416
MluI A'CGCG_T
Cuts at: 5292 5292
Size: 5416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2349 4530 4714 1305
Size: 1044 2181 184 2007
Fragments arranged by size:
2181 2007 1044 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3594
Fragments arranged by size:
3594 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2238 2387 3374 3733 3892 518
Size: 648 1072 149 987 359 159 2042
Fragments arranged by size:
2042 1072 987 648 359 159 149
MspA1I CmG'CkG
Cuts at: 1606 2042 2832 3509 4657 4902 1606
Size: 436 790 677 1148 245 2120
Fragments arranged by size:
2120 1148 790 677 436 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4864
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5416
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5416
NotI GC'GGCC_GC
Cuts at: 2863 2863
Size: 5416
NsiI A_TGCA'T
Cuts at: 2200 2200
Size: 5416
NspI r_CATG'y
Cuts at: 1132 1229 2198 2774 4319 1132
Size: 97 969 576 1545 2229
Fragments arranged by size:
2229 1545 969 576 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5416
PciI A'CATG_T
Cuts at: 4315 4315
Size: 5416
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5416
PmeI GTTT'AAAC
Cuts at: 2720 2720
Size: 5416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2363 2395 3485 3517 4998 5030 2363
Size: 32 1090 32 1481 32 2749
Fragments arranged by size:
2749 1481 1090 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5416
PsiI TTA'TAA
Cuts at: 2986 2986
Size: 5416
PstI C_TGCA'G
Cuts at: 830 2768 830
Size: 1938 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5416
SalI G'TCGA_C
Cuts at: 1936 2758 1936
Size: 822 4594
SbfI CC_TGCA'GG
Cuts at: 2768 2768
Size: 5416
ScaI AGT'ACT
Cuts at: 3664 5376 3664
Size: 1712 3704
SfcI C'TryA_G
Cuts at: 826 1045 2764 3899 4580 4771 826
Size: 219 1719 1135 681 191 1471
Fragments arranged by size:
1719 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5416
SmaI CCC'GGG
Cuts at: 1872 2743 1872
Size: 871 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3520 4421 4683 820
Size: 197 926 1577 901 262 1553
Fragments arranged by size:
1577 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3207 493
Size: 2714 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5416
SphI G_CATG'C
Cuts at: 1132 2774 1132
Size: 1642 3774
SspI AAT'ATT
Cuts at: 5 52 3340 5
Size: 47 3288 2081
Fragments arranged by size:
3288 2081 47
StuI AGG'CCT
Cuts at: 1517 2405 1517
Size: 888 4528
StyI C'CwwG_G
Cuts at: 513 1065 2855 513
Size: 552 1790 3074
Fragments arranged by size:
3074 1790 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2226 3443 3460 3613 3798 4243 325
Size: 1255 646 1217 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1217 646 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3662 5374 96
Size: 274 80 33 51 1443 1685 1712 138
Fragments arranged by size:
1712 1685 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5376 5408 5376
Size: 32 5384
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5416
XbaI T'CTAG_A
Cuts at: 2752 2752
Size: 5416
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5416
XmaI C'CCGG_G
Cuts at: 1870 2741 1870
Size: 871 4545
XmnI GAAnn'nnTTC
Cuts at: 2578 3545 2578
Size: 967 4449
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3224 276
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI