(Circular) (Six-base) MAPSORT of: pFN21AE2293.seq Check: 9052 from: 1 to: 5323 pFN21AE2293 5323 bp With 182 enzymes: SgfI * March 28, 2012 12:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3133 278 Size: 53 83 186 936 1597 2468 Fragments arranged by size: 2468 1597 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2666 1937 Size: 729 4594 Acc65I G'GTAC_C Cuts at: 2644 2644 Size: 5323 AclI AA'CG_TT Cuts at: 1563 3450 3823 1563 Size: 1887 373 3063 Fragments arranged by size: 3063 1887 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3386 4770 5038 1632 Size: 1754 1384 268 1917 Fragments arranged by size: 1917 1754 1384 268 AfeI AGC'GCT Cuts at: 2034 2034 Size: 5323 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5126 AflIII A'CryG_T Cuts at: 1203 4222 5199 1203 Size: 3019 977 1327 Fragments arranged by size: 3019 1327 977 AgeI A'CCGG_T Cuts at: 4154 4154 Size: 5323 AhdI GACnn_n'nnGTC Cuts at: 4052 4052 Size: 5323 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5323 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5323 AlwNI CAG_nnn'CTG Cuts at: 3066 4638 3066 Size: 1572 3751 ApaI G_GGCC'C Cuts at: 2016 2016 Size: 5323 ApaLI G'TGCA_C Cuts at: 2467 3380 4536 2467 Size: 913 1156 3254 Fragments arranged by size: 3254 1156 913 ApoI r'AATT_y Cuts at: 1493 2632 2858 3010 5119 1493 Size: 1139 226 152 2109 1697 Fragments arranged by size: 2109 1697 1139 226 152 AscI GG'CGCG_CC Cuts at: 2171 2171 Size: 5323 AseI AT'TA_AT Cuts at: 160 1032 3877 160 Size: 872 2845 1606 Fragments arranged by size: 2845 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5323 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2648 1870 Size: 73 72 633 4545 Fragments arranged by size: 4545 633 73 72 BamHI G'GATC_C Cuts at: 2653 3020 2653 Size: 367 4956 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 2087 2219 2644 4099 Size: 325 217 627 278 22 132 425 1455 Cuts at: 4099 618 Size: 1842 Fragments arranged by size: 1842 1455 627 425 325 278 217 132 22 BanII G_rGCy'C Cuts at: 1391 2016 2358 2642 1391 Size: 625 342 284 4072 Fragments arranged by size: 4072 625 342 284 BbeI G_GCGC'C Cuts at: 2069 2091 2223 2069 Size: 22 132 5169 Fragments arranged by size: 5169 132 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2567 928 Size: 971 668 3684 Fragments arranged by size: 3684 971 668 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2521 2555 3514 3548 1903 Size: 34 584 34 959 34 3678 Fragments arranged by size: 3678 959 584 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3215 4425 3215 Size: 1210 4113 BclI T'GATC_A Cuts at: 1548 1548 Size: 5323 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2063 2085 2316 Size: 107 122 71 631 605 391 22 231 Cuts at: 2316 3934 136 Size: 1618 1525 Fragments arranged by size: 1618 1525 631 605 391 231 122 107 71 22 BglII A'GATC_T Cuts at: 5318 5318 Size: 5323 BlpI GC'TnA_GC Cuts at: 2740 2740 Size: 5323 Bme1580I G_kGCm'C Cuts at: 1790 2016 2471 3384 4540 1790 Size: 226 455 913 1156 2573 Fragments arranged by size: 2573 1156 913 455 226 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5323 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3054 4012 442 Size: 416 53 83 399 24 1637 958 1753 Fragments arranged by size: 1753 1637 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5323 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2153 2276 2372 3983 1239 Size: 902 12 123 96 1611 2579 Fragments arranged by size: 2579 1611 902 123 96 12 Bpu10I CC'TnA_GC Cuts at: 2546 2546 Size: 5323 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3448 4313 4611 3448 Size: 865 298 4160 Fragments arranged by size: 4160 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3986 882 Size: 617 2487 2219 Fragments arranged by size: 2487 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3114 493 Size: 711 1910 2702 Fragments arranged by size: 2702 1910 711 BsaBI GATnn'nnATC Cuts at: 5317 5317 Size: 5323 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 2088 2220 3130 Size: 53 83 186 936 533 22 132 910 Cuts at: 3130 3512 4147 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 910 635 533 382 186 132 83 53 22 BsaWI w'CCGG_w Cuts at: 3756 4154 4428 4575 4986 3756 Size: 398 274 147 411 4093 Fragments arranged by size: 4093 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5098 Fragments arranged by size: 5098 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5323 BseYI C'CCAG_C Cuts at: 2142 2236 4526 5022 2142 Size: 94 2290 496 2443 Fragments arranged by size: 2443 2290 496 94 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2088 1163 Size: 573 352 4398 Fragments arranged by size: 4398 573 352 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 2208 2311 2773 3534 4562 Size: 275 186 24 183 103 462 761 1028 Cuts at: 4562 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 462 275 186 183 103 24 BsiHKAI G_wGCw'C Cuts at: 2358 2471 3384 3469 4540 2358 Size: 113 913 85 1071 3141 Fragments arranged by size: 3141 1071 913 113 85 BsmI GAATG_Cn' Cuts at: 2834 2927 2834 Size: 93 5230 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2358 2471 2642 3384 3469 4540 Size: 399 226 342 113 171 742 85 1071 Cuts at: 4540 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 342 226 171 113 85 BspEI T'CCGG_A Cuts at: 4986 4986 Size: 5323 BspHI T'CATG_A Cuts at: 3212 4942 3212 Size: 1730 3593 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2241 2678 844 Size: 1397 437 3489 Fragments arranged by size: 3489 1397 437 BsrBI CCG'CTC Cuts at: 1394 1823 2217 2371 3210 1394 Size: 429 394 154 839 3507 Fragments arranged by size: 3507 839 429 394 154 BsrDI GCAATG_nn' Cuts at: 65 1248 3818 3992 65 Size: 1183 2570 174 1396 Fragments arranged by size: 2570 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2179 2222 2403 3967 4154 1700 Size: 110 369 43 181 1564 187 2869 Fragments arranged by size: 2869 1564 369 187 181 110 43 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5323 BssHII G'CGCG_C Cuts at: 1925 2171 2322 1925 Size: 246 151 4926 Fragments arranged by size: 4926 246 151 BssSI C'ACGA_G Cuts at: 3383 4395 3383 Size: 1012 4311 BstAPI GCAn_nnn'nTGC Cuts at: 794 2102 794 Size: 1308 4015 BstBI TT'CG_AA Cuts at: 4127 4127 Size: 5323 BstEII G'GTnAC_C Cuts at: 1184 2599 1184 Size: 1415 3908 BstYI r'GATC_y Cuts at: 1972 2653 3020 3405 3422 4874 4960 4972 5318 Size: 681 367 385 17 1452 86 12 346 Cuts at: 5318 1972 Size: 1977 Fragments arranged by size: 1977 1452 681 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2025 2364 513 Size: 552 960 339 3472 Fragments arranged by size: 3472 960 552 339 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2524 487 Size: 683 56 1298 3286 Fragments arranged by size: 3286 1298 683 56 BtsI GCAGTG_nn' Cuts at: 2100 2839 3633 3653 2100 Size: 739 794 20 3770 Fragments arranged by size: 3770 794 739 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5323 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5288 DraI TTT'AAA Cuts at: 2627 2627 Size: 5323 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4330 809 Size: 507 3014 1802 Fragments arranged by size: 3014 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2205 2308 Size: 54 1425 50 275 18 192 183 103 Cuts at: 2308 2397 2415 2770 3659 5288 8 Size: 89 18 355 889 1629 43 Fragments arranged by size: 1629 1425 889 355 275 192 183 103 89 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2022 2205 2308 2770 1537 Size: 275 210 183 103 462 4090 Fragments arranged by size: 4090 462 275 210 183 103 EarI CTCTTCn'nnn_ Cuts at: 1353 3253 1353 Size: 1900 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3910 4284 4430 387 Size: 1064 138 2321 374 146 1280 Fragments arranged by size: 2321 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2356 2356 Size: 5323 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2153 2276 2372 3386 3983 4770 Size: 393 509 12 123 96 1014 597 787 Cuts at: 4770 5038 1239 Size: 268 1524 Fragments arranged by size: 1524 1014 787 597 509 393 268 123 96 12 EcoO109I rG'GnC_Cy Cuts at: 2012 2012 Size: 5323 EcoRI G'AATT_C Cuts at: 2632 3010 2632 Size: 378 4945 FspI TGC'GCA Cuts at: 3829 3829 Size: 5323 HaeII r_GCGC'y Cuts at: 1678 2036 2069 2091 2140 2162 2223 2300 4470 Size: 358 33 22 49 22 61 77 2170 Cuts at: 4470 1678 Size: 2531 Fragments arranged by size: 2531 2170 358 77 61 49 33 22 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3964 3996 4038 4070 1373 Size: 32 135 32 2392 32 42 32 2626 Fragments arranged by size: 2626 2392 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2667 2913 669 Size: 988 281 729 246 3079 Fragments arranged by size: 3079 988 729 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5323 HpaI GTT'AAC Cuts at: 2913 2913 Size: 5323 KasI G'GCGC_C Cuts at: 2065 2087 2219 2065 Size: 22 132 5169 Fragments arranged by size: 5169 132 22 KpnI G_GTAC'C Cuts at: 2648 2648 Size: 5323 MfeI C'AATT_G Cuts at: 2922 2922 Size: 5323 MluI A'CGCG_T Cuts at: 5199 5199 Size: 5323 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4437 4621 1305 Size: 3132 184 2007 Fragments arranged by size: 3132 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2399 2417 10 Size: 54 1425 343 567 18 2916 Fragments arranged by size: 2916 1425 567 343 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 3281 3640 3799 518 Size: 648 2115 359 159 2042 Fragments arranged by size: 2115 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 2142 2739 3416 4564 4809 1606 Size: 421 115 597 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 597 421 245 115 NaeI GCC'GGC Cuts at: 2224 2224 Size: 5323 NarI GG'CG_CC Cuts at: 2066 2088 2220 2066 Size: 22 132 5169 Fragments arranged by size: 5169 132 22 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4771 NdeI CA'TA_TG Cuts at: 387 387 Size: 5323 NgoMIV G'CCGG_C Cuts at: 2222 2222 Size: 5323 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5323 NotI GC'GGCC_GC Cuts at: 2770 2770 Size: 5323 NspI r_CATG'y Cuts at: 1132 1229 2536 2681 4226 1132 Size: 97 1307 145 1545 2229 Fragments arranged by size: 2229 1545 1307 145 97 PasI CC'CwG_GG Cuts at: 1348 2070 1348 Size: 722 4601 PciI A'CATG_T Cuts at: 4222 4222 Size: 5323 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5323 PmeI GTTT'AAAC Cuts at: 2627 2627 Size: 5323 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2456 2488 3392 3424 4905 4937 2456 Size: 32 904 32 1481 32 2842 Fragments arranged by size: 2842 1481 904 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5323 PsiI TTA'TAA Cuts at: 2893 2893 Size: 5323 PspOMI G'GGCC_C Cuts at: 2012 2012 Size: 5323 PstI C_TGCA'G Cuts at: 830 2675 830 Size: 1845 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5323 SacI G_AGCT'C Cuts at: 2358 2358 Size: 5323 SacII CC_GC'GG Cuts at: 2028 2028 Size: 5323 SalI G'TCGA_C Cuts at: 1936 2665 1936 Size: 729 4594 SbfI CC_TGCA'GG Cuts at: 2675 2675 Size: 5323 ScaI AGT'ACT Cuts at: 3571 5283 3571 Size: 1712 3611 SfcI C'TryA_G Cuts at: 826 1045 2671 3806 4487 4678 826 Size: 219 1626 1135 681 191 1471 Fragments arranged by size: 1626 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2067 2089 2221 2067 Size: 22 132 5169 Fragments arranged by size: 5169 132 22 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5323 SgrAI Cr'CCGG_yG Cuts at: 1700 2222 1700 Size: 522 4801 SmaI CCC'GGG Cuts at: 1872 2017 2650 1872 Size: 145 633 4545 Fragments arranged by size: 4545 633 145 SmlI C'TyrA_G Cuts at: 820 1017 1943 3427 4328 4590 820 Size: 197 926 1484 901 262 1553 Fragments arranged by size: 1553 1484 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3114 493 Size: 2621 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5323 SphI G_CATG'C Cuts at: 1132 2681 1132 Size: 1549 3774 SspI AAT'ATT Cuts at: 5 52 3247 5 Size: 47 3195 2081 Fragments arranged by size: 3195 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5323 StyI C'CwwG_G Cuts at: 513 1065 2196 2762 513 Size: 552 1131 566 3074 Fragments arranged by size: 3074 1131 566 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2113 2122 2618 3350 3367 3520 3705 Size: 1255 533 9 496 732 17 153 185 Cuts at: 3705 4150 325 Size: 445 1498 Fragments arranged by size: 1498 1255 732 533 496 445 185 153 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3569 5281 96 Size: 274 80 33 51 1443 1592 1712 138 Fragments arranged by size: 1712 1592 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5283 5315 5283 Size: 32 5291 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5323 XbaI T'CTAG_A Cuts at: 2659 2659 Size: 5323 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2116 2116 Size: 5323 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5323 XmaI C'CCGG_G Cuts at: 1870 2015 2648 1870 Size: 145 633 4545 Fragments arranged by size: 4545 633 145 XmnI GAAnn'nnTTC Cuts at: 3452 3452 Size: 5323 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3131 276 Size: 53 83 186 936 1597 2468 Fragments arranged by size: 2468 1597 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI