################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AvaI AvrII BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspMI BsrDI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI HincII HindII HindIII HpaI KspI MaeI MamI MfeI MluI MroI MseI MslI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp509I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 296 GGTACC G^GTACC AflIII 557 ACATGT A^C[GA][CT]GT AlwNI 328 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 91 GGGCCC GGGCC^C Asp718I 296 GGTACC G^GTACC AspI 193 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 179 TGCGCA TGC^GCA BalI 269 TGGCCA TGG^CCA BamHI 32 GGATCC G^GATCC BbrPI 97 CACGTG CAC^GTG BbsI 352 GAAGAC GAAGAC(2/6) BglI 380 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 352 GAAGAC GAAGAC(2/6) BsaWI 610 ACCGGA [AT]^CCGG[AT] BspLU11I 557 ACATGT A^CATGT BsrBI 6 GAGCGG CCGCTC(-3/-3) BsrFI 40 GCCGGC [GA]^CCGG[CT] BsrI 615 ACTGG ACTGG(1/-1) Cfr10I 40 GCCGGC [GA]^CCGG[CT] CpoI 262 CGGACCG CG^G[AT]CCG DraIII 286 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 564 CTCTTC CTCTTC(1/4) EcoT14I 509 CCATGG C^C[AT][AT]GG FseI 39 GGCCGGCC GGCCGG^CC FspI 179 TGCGCA TGC^GCA HphI 608 TCACC GGTGA(8/7) KpnI 296 GGTACC GGTAC^C Ksp632I 564 CTCTTC CTCTTC(1/4) MaeIII 607 GTCAC ^GT[ACGT]AC MluNI 269 TGGCCA TGG^CCA MscI 269 TGGCCA TGG^CCA NaeI 40 GCCGGC GCC^GGC NcoI 509 CCATGG C^CATGG NgoMIV 40 GCCGGC G^CCGGC NspI 557 ACATGT [GA]CATG^[CT] PflFI 193 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 630 GAGTC GAGTC(4/5) PmaCI 97 CACGTG CAC^GTG PmlI 97 CACGTG CAC^GTG PpuMI 530 AGGACCT [GA]G^G[AT]CC[CT] RsrII 262 CGGACCG CG^G[AT]CCG ScaI 341 AGTACT AGT^ACT StyI 509 CCATGG C^C[AT][AT]GG TaqI 365 TCGA T^CGA TfiI 550 GAATC G^A[AT]TC Tsp45I 607 GTCAC ^GT[GC]AC TspRI 438 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 193 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 365 TCGA T^CGA XcmI 500 CCATGCTGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### Alw44I 100 GTGCAC G^TGCAC 283 GTGCAC AocI 526 CCTGAGG CC^T[ACGT]AGG 534 CCTCAGG ApaLI 100 GTGCAC G^TGCAC 283 GTGCAC AspHI 100 GTGCAC G[AT]GC[AT]^C 283 GTGCAC BanII 91 GGGCCC G[GA]GC[CT]^C 160 GGGCTC BbeI 65 GGCGCC GGCGC^C 310 GGCGCC BcnI 62 CCGGG CC^[GC]GG 423 CCCGG BsaAI 25 CACGTA [CT]AC^GT[GA] 97 CACGTG BsaI 577 GAGACC GGTCTC(1/5) 426 GGTCTC BsiHKAI 100 GTGCAC G[AT]GC[AT]^C 283 GTGCAC BsmAI 577 GAGAC GTCTC(1/5) 427 GTCTC BsmFI 514 GTCCC GGGAC(10/14) 632 GTCCC Bsp1407I 140 TGTACA T^GTACA 458 TGTACA BsrGI 140 TGTACA T^GTACA 458 TGTACA BssHII 209 GCGCGC G^CGCGC 224 GCGCGC BstF5I 123 CATCC GGATG(2/0) 183 CATCC BstNI 36 CCTGG CC^[AT]GG 573 CCTGG BstYI 32 GGATCC [GA]^GATC[CT] 374 AGATCC Bsu36I 526 CCTGAGG CC^T[ACGT]AGG 534 CCTCAGG Eco81I 526 CCTGAGG CC^T[ACGT]AGG 534 CCTCAGG EcoRII 36 CCTGG ^CC[AT]GG 573 CCTGG FokI 123 CATCC GGATG(9/13) 183 CATCC HaeII 65 GGCGCC [GA]GCGC^[CT] 310 GGCGCC HgaI 85 GACGC GACGC(5/10) 197 GCGTC HinfI 550 GAATC G^A[ACGT]TC 630 GAGTC KasI 65 GGCGCC G^GCGCC 310 GGCGCC MaeII 26 ACGT A^CGT 98 ACGT MflI 32 GGATCC [GA]^GATC[CT] 374 AGATCC MvaI 36 CCTGG CC^[AT]GG 573 CCTGG NarI 65 GGCGCC GG^CGCC 310 GGCGCC NciI 62 CCGGG CC^[GC]GG 423 CCCGG PvuII 168 CAGCTG CAG^CTG 454 CAGCTG SfaNI 324 GATGC GCATC(5/9) 182 GCATC SfoI 65 GGCGCC GGC^GCC 310 GGCGCC SspBI 140 TGTACA T^GTACA 458 TGTACA XhoII 32 GGATCC [GA]^GATC[CT] 374 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaII BanI BbvI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstAPI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I FauI Fnu4HI HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MboI MboII MnlI MspA1I MspI MvnI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI