(Circular) (Six-base) MAPSORT of: pFN21AE1713.seq Check: 1593 from: 1 to: 5353 pFN21AE1713 5353 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3163 278 Size: 53 83 186 936 1627 2468 Fragments arranged by size: 2468 1627 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2696 1937 Size: 759 4594 Acc65I G'GTAC_C Cuts at: 2301 2674 2301 Size: 373 4980 AclI AA'CG_TT Cuts at: 1563 3480 3853 1563 Size: 1917 373 3063 Fragments arranged by size: 3063 1917 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 3416 4800 5068 1632 Size: 695 1089 1384 268 1917 Fragments arranged by size: 1917 1384 1089 695 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5156 AflIII A'CryG_T Cuts at: 1203 2562 4252 5229 1203 Size: 1359 1690 977 1327 Fragments arranged by size: 1690 1359 1327 977 AgeI A'CCGG_T Cuts at: 4184 4184 Size: 5353 AhdI GACnn_n'nnGTC Cuts at: 4082 4082 Size: 5353 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5353 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5353 AlwNI CAG_nnn'CTG Cuts at: 2338 3096 4668 2338 Size: 758 1572 3023 Fragments arranged by size: 3023 1572 758 ApaI G_GGCC'C Cuts at: 2100 2100 Size: 5353 ApaLI G'TGCA_C Cuts at: 2105 2288 3410 4566 2105 Size: 183 1122 1156 2892 Fragments arranged by size: 2892 1156 1122 183 ApoI r'AATT_y Cuts at: 1493 2662 2888 3040 5149 1493 Size: 1169 226 152 2109 1697 Fragments arranged by size: 2109 1697 1169 226 152 AseI AT'TA_AT Cuts at: 160 1032 3907 160 Size: 872 2875 1606 Fragments arranged by size: 2875 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5353 AvaI C'yCGr_G Cuts at: 1870 1943 2678 1870 Size: 73 735 4545 Fragments arranged by size: 4545 735 73 BamHI G'GATC_C Cuts at: 2037 2683 3050 2037 Size: 646 367 4340 Fragments arranged by size: 4340 646 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2070 2139 2301 2315 2674 Size: 325 217 627 283 69 162 14 359 Cuts at: 2674 4129 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 359 325 283 217 162 69 14 BanII G_rGCy'C Cuts at: 1391 2100 2169 2672 1391 Size: 709 69 503 4072 Fragments arranged by size: 4072 709 503 69 BbeI G_GCGC'C Cuts at: 2074 2319 2074 Size: 245 5108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2364 928 Size: 971 465 3917 Fragments arranged by size: 3917 971 465 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3544 3578 1903 Size: 34 50 34 1523 34 3678 Fragments arranged by size: 3678 1523 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3245 4455 3245 Size: 1210 4143 BclI T'GATC_A Cuts at: 1548 1548 Size: 5353 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2391 3964 136 Size: 107 122 71 631 605 719 1573 1525 Fragments arranged by size: 1573 1525 719 631 605 122 107 71 BglII A'GATC_T Cuts at: 5348 5348 Size: 5353 BlpI GC'TnA_GC Cuts at: 2770 2770 Size: 5353 Bme1580I G_kGCm'C Cuts at: 1790 2100 2109 2292 3414 4570 1790 Size: 310 9 183 1122 1156 2573 Fragments arranged by size: 2573 1156 1122 310 183 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5353 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3084 4042 442 Size: 416 53 83 399 24 1667 958 1753 Fragments arranged by size: 1753 1667 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5353 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2155 2264 2600 2642 4013 1239 Size: 916 109 336 42 1371 2579 Fragments arranged by size: 2579 1371 916 336 109 42 Bpu10I CC'TnA_GC Cuts at: 2438 2438 Size: 5353 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3478 4343 4641 3478 Size: 865 298 4190 Fragments arranged by size: 4190 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2437 2576 4016 882 Size: 617 938 139 1440 2219 Fragments arranged by size: 2219 1440 938 617 139 BsaAI yAC'GTr Cuts at: 493 1204 2032 2104 3144 493 Size: 711 828 72 1040 2702 Fragments arranged by size: 2702 1040 828 711 72 BsaBI GATnn'nnATC Cuts at: 5347 5347 Size: 5353 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2071 2202 2316 3160 Size: 53 83 186 936 538 131 114 844 Cuts at: 3160 3542 4177 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 844 635 538 382 186 131 114 83 53 BsaWI w'CCGG_w Cuts at: 2615 3786 4184 4458 4605 5016 2615 Size: 1171 398 274 147 411 2952 Fragments arranged by size: 2952 1171 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2002 2032 2153 2183 1178 Size: 30 165 30 599 30 121 30 4348 Fragments arranged by size: 4348 599 165 121 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2387 2390 2540 2647 1186 Size: 1201 3 150 107 3892 Fragments arranged by size: 3892 1201 150 107 3 BseYI C'CCAG_C Cuts at: 2115 2639 4556 5052 2115 Size: 524 1917 496 2416 Fragments arranged by size: 2416 1917 524 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4780 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2803 3564 4592 1540 Size: 275 186 802 761 1028 2301 Fragments arranged by size: 2301 1028 802 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2109 2292 3414 3499 4570 2109 Size: 183 1122 85 1071 2892 Fragments arranged by size: 2892 1122 1071 183 85 BsmI GAATG_Cn' Cuts at: 2864 2957 2864 Size: 93 5260 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2109 2169 2292 2672 3414 3499 Size: 399 310 9 60 123 380 742 85 Cuts at: 3499 4570 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 380 310 123 85 60 9 BspEI T'CCGG_A Cuts at: 5016 5016 Size: 5353 BspHI T'CATG_A Cuts at: 3242 4972 3242 Size: 1730 3623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2708 844 Size: 1864 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2013 3240 1394 Size: 429 190 1227 3507 Fragments arranged by size: 3507 1227 429 190 BsrDI GCAATG_nn' Cuts at: 65 1248 3848 4022 65 Size: 1183 2600 174 1396 Fragments arranged by size: 2600 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2045 3997 4184 1700 Size: 110 235 1952 187 2869 Fragments arranged by size: 2869 1952 235 187 110 BsrGI T'GTAC_A Cuts at: 96 2145 2463 96 Size: 2049 318 2986 Fragments arranged by size: 2986 2049 318 BssHII G'CGCG_C Cuts at: 1925 2214 2229 1925 Size: 289 15 5049 Fragments arranged by size: 5049 289 15 BssSI C'ACGA_G Cuts at: 3413 4425 3413 Size: 1012 4341 BstAPI GCAn_nnn'nTGC Cuts at: 794 2239 2296 2338 794 Size: 1445 57 42 3809 Fragments arranged by size: 3809 1445 57 42 BstBI TT'CG_AA Cuts at: 4157 4157 Size: 5353 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5353 BstYI r'GATC_y Cuts at: 1972 2037 2379 2683 3050 3435 3452 4904 4990 Size: 65 342 304 367 385 17 1452 86 Cuts at: 4990 5002 5348 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 342 304 86 65 17 12 Bsu36I CC'TnA_GG Cuts at: 2532 2540 2532 Size: 8 5345 BtgI C'CryG_G Cuts at: 513 1065 2061 2161 2271 2514 513 Size: 552 996 100 110 243 3352 Fragments arranged by size: 3352 996 552 243 110 100 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4614 Fragments arranged by size: 4614 683 56 BtsI GCAGTG_nn' Cuts at: 2449 2869 3663 3683 2449 Size: 420 794 20 4119 Fragments arranged by size: 4119 794 420 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5353 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5318 DraI TTT'AAA Cuts at: 2657 2657 Size: 5353 DraIII CAC_nnn'GTG Cuts at: 2296 2296 Size: 5353 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4360 809 Size: 507 3044 1802 Fragments arranged by size: 3044 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2058 2064 Size: 54 1425 50 275 18 213 15 6 Cuts at: 2064 2274 2800 3689 5318 8 Size: 210 526 889 1629 43 Fragments arranged by size: 1629 1425 889 526 275 213 210 54 50 43 18 15 6 EagI C'GGCC_G Cuts at: 1537 1812 2800 1537 Size: 275 988 4090 Fragments arranged by size: 4090 988 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2575 3283 1353 Size: 1222 708 3423 Fragments arranged by size: 3423 1222 708 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2120 2372 3940 4314 4460 387 Size: 1064 138 531 252 1568 374 146 1280 Fragments arranged by size: 1568 1280 1064 531 374 252 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2155 2264 2327 2600 2642 3416 4013 Size: 393 523 109 63 273 42 774 597 Cuts at: 4013 4800 5068 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 774 597 523 393 273 268 109 63 42 EcoO109I rG'GnC_Cy Cuts at: 2096 2097 2536 2096 Size: 1 439 4913 Fragments arranged by size: 4913 439 1 EcoRI G'AATT_C Cuts at: 2662 3040 2662 Size: 378 4975 FseI GG_CCGG'CC Cuts at: 2049 2049 Size: 5353 FspI TGC'GCA Cuts at: 2186 3859 2186 Size: 1673 3680 HaeII r_GCGC'y Cuts at: 1678 2074 2319 4500 1678 Size: 396 245 2181 2531 Fragments arranged by size: 2531 2181 396 245 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2584 3994 4026 4068 Size: 32 135 32 980 32 1410 32 42 Cuts at: 4068 4100 1373 Size: 32 2626 Fragments arranged by size: 2626 1410 980 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2697 2943 669 Size: 988 281 759 246 3079 Fragments arranged by size: 3079 988 759 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5353 HpaI GTT'AAC Cuts at: 2943 2943 Size: 5353 KasI G'GCGC_C Cuts at: 2070 2315 2070 Size: 245 5108 KpnI G_GTAC'C Cuts at: 2305 2678 2305 Size: 373 4980 MfeI C'AATT_G Cuts at: 2952 2952 Size: 5353 MluI A'CGCG_T Cuts at: 5229 5229 Size: 5353 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4467 4651 1305 Size: 3162 184 2007 Fragments arranged by size: 3162 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2276 10 Size: 54 1425 343 444 3087 Fragments arranged by size: 3087 1425 444 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3311 3670 3829 518 Size: 648 2145 359 159 2042 Fragments arranged by size: 2145 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2175 2455 2461 2769 3446 4594 4839 1606 Size: 569 280 6 308 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 569 308 280 245 6 NaeI GCC'GGC Cuts at: 2047 2047 Size: 5353 NarI GG'CG_CC Cuts at: 2071 2316 2071 Size: 245 5108 NcoI C'CATG_G Cuts at: 513 1065 2514 513 Size: 552 1449 3352 Fragments arranged by size: 3352 1449 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5353 NgoMIV G'CCGG_C Cuts at: 2045 2045 Size: 5353 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5353 NotI GC'GGCC_GC Cuts at: 2800 2800 Size: 5353 NspI r_CATG'y Cuts at: 1132 1229 2566 2711 4256 1132 Size: 97 1337 145 1545 2229 Fragments arranged by size: 2229 1545 1337 145 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5353 PciI A'CATG_T Cuts at: 2562 4252 2562 Size: 1690 3663 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5353 PmeI GTTT'AAAC Cuts at: 2657 2657 Size: 5353 PmlI CAC'GTG Cuts at: 2104 2104 Size: 5353 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3422 3454 4935 4967 3422 Size: 32 1481 32 3808 Fragments arranged by size: 3808 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2536 2536 Size: 5353 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5353 PsiI TTA'TAA Cuts at: 2923 2923 Size: 5353 PspOMI G'GGCC_C Cuts at: 2096 2096 Size: 5353 PstI C_TGCA'G Cuts at: 830 2705 830 Size: 1875 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5353 PvuII CAG'CTG Cuts at: 2175 2461 2175 Size: 286 5067 RsrII CG'GwC_CG Cuts at: 2268 2268 Size: 5353 SalI G'TCGA_C Cuts at: 1936 2695 1936 Size: 759 4594 SbfI CC_TGCA'GG Cuts at: 2705 2705 Size: 5353 ScaI AGT'ACT Cuts at: 2348 3601 5313 2348 Size: 1253 1712 2388 Fragments arranged by size: 2388 1712 1253 SfcI C'TryA_G Cuts at: 826 1045 2701 3836 4517 4708 826 Size: 219 1656 1135 681 191 1471 Fragments arranged by size: 1656 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2072 2317 2072 Size: 245 5108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5353 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5353 SmaI CCC'GGG Cuts at: 1872 2680 1872 Size: 808 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3457 4358 4620 820 Size: 197 926 1514 901 262 1553 Fragments arranged by size: 1553 1514 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3144 493 Size: 2651 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5353 SphI G_CATG'C Cuts at: 1132 2711 1132 Size: 1579 3774 SspI AAT'ATT Cuts at: 5 52 3277 5 Size: 47 3225 2081 Fragments arranged by size: 3225 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5353 StyI C'CwwG_G Cuts at: 513 1065 2514 2792 513 Size: 552 1449 278 3074 Fragments arranged by size: 3074 1449 552 278 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2600 3380 3397 3550 3735 4180 325 Size: 1255 1020 780 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1020 780 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 2346 2463 Size: 274 80 33 51 1443 168 201 117 Cuts at: 2463 3599 5311 96 Size: 1136 1712 138 Fragments arranged by size: 1712 1443 1136 274 201 168 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2002 2034 2153 2185 5313 5345 2002 Size: 32 119 32 3128 32 2010 Fragments arranged by size: 3128 2010 119 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2201 1316 Size: 885 4468 XbaI T'CTAG_A Cuts at: 2689 2689 Size: 5353 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2512 2512 Size: 5353 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5353 XmaI C'CCGG_G Cuts at: 1870 2678 1870 Size: 808 4545 XmnI GAAnn'nnTTC Cuts at: 3482 3482 Size: 5353 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3161 276 Size: 53 83 186 936 1627 2468 Fragments arranged by size: 2468 1627 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SrfI SwaI