(Linear) (Six-base) MAPSORT of: KIEE1713.seq Check: 645 from: 1 to: 648 KIEE1713.orf With 182 enzymes: SgfI * July 15, 2010 18:45 .. Acc65I G'GTAC_C Cuts at: 0 296 648 Size: 296 352 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 648 Size: 322 326 AflIII A'CryG_T Cuts at: 0 557 648 Size: 557 91 AlwNI CAG_nnn'CTG Cuts at: 0 333 648 Size: 333 315 ApaI G_GGCC'C Cuts at: 0 95 648 Size: 95 553 ApaLI G'TGCA_C Cuts at: 0 100 283 648 Size: 100 183 365 Fragments arranged by size: 365 183 100 BamHI G'GATC_C Cuts at: 0 32 648 Size: 32 616 BanI G'GyrC_C Cuts at: 0 65 134 296 310 648 Size: 65 69 162 14 338 Fragments arranged by size: 338 162 69 65 14 BanII G_rGCy'C Cuts at: 0 95 164 648 Size: 95 69 484 Fragments arranged by size: 484 95 69 BbeI G_GCGC'C Cuts at: 0 69 314 648 Size: 69 245 334 Fragments arranged by size: 334 245 69 BbsI GAAGACnn'nnnn_ Cuts at: 0 359 648 Size: 359 289 BglI GCCn_nnn'nGGC Cuts at: 0 386 648 Size: 386 262 Bme1580I G_kGCm'C Cuts at: 0 95 104 287 648 Size: 95 9 183 361 Fragments arranged by size: 361 183 95 9 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 150 259 595 637 648 Size: 150 109 336 42 11 Fragments arranged by size: 336 150 109 42 11 Bpu10I CC'TnA_GC Cuts at: 0 433 648 Size: 433 215 BsaI GGTCTCn'nnnn_ Cuts at: 0 432 571 648 Size: 432 139 77 Fragments arranged by size: 432 139 77 BsaAI yAC'GTr Cuts at: 0 27 99 648 Size: 27 72 549 Fragments arranged by size: 549 72 27 BsaHI Gr'CG_yC Cuts at: 0 66 197 311 648 Size: 66 131 114 337 Fragments arranged by size: 337 131 114 66 BsaWI w'CCGG_w Cuts at: 0 610 648 Size: 610 38 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 27 148 178 648 Size: 27 121 30 470 Fragments arranged by size: 470 121 30 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 382 385 535 642 648 Size: 382 3 150 107 6 Fragments arranged by size: 382 150 107 6 3 BseYI C'CCAG_C Cuts at: 0 110 634 648 Size: 110 524 14 Fragments arranged by size: 524 110 14 BsiHKAI G_wGCw'C Cuts at: 0 104 287 648 Size: 104 183 361 Fragments arranged by size: 361 183 104 Bsp1286I G_dGCh'C Cuts at: 0 95 104 164 287 648 Size: 95 9 60 123 361 Fragments arranged by size: 361 123 95 60 9 BsrBI CCG'CTC Cuts at: 0 8 648 Size: 8 640 BsrFI r'CCGG_y Cuts at: 0 40 648 Size: 40 608 BsrGI T'GTAC_A Cuts at: 0 140 458 648 Size: 140 318 190 Fragments arranged by size: 318 190 140 BssHII G'CGCG_C Cuts at: 0 209 224 648 Size: 209 15 424 Fragments arranged by size: 424 209 15 BstAPI GCAn_nnn'nTGC Cuts at: 0 234 291 333 648 Size: 234 57 42 315 Fragments arranged by size: 315 234 57 42 BstYI r'GATC_y Cuts at: 0 32 374 648 Size: 32 342 274 Fragments arranged by size: 342 274 32 Bsu36I CC'TnA_GG Cuts at: 0 527 535 648 Size: 527 8 113 Fragments arranged by size: 527 113 8 BtgI C'CryG_G Cuts at: 0 56 156 266 509 648 Size: 56 100 110 243 139 Fragments arranged by size: 243 139 110 100 56 BtsI GCAGTG_nn' Cuts at: 0 444 648 Size: 444 204 DraIII CAC_nnn'GTG Cuts at: 0 291 648 Size: 291 357 EaeI y'GGCC_r Cuts at: 0 38 53 59 269 648 Size: 38 15 6 210 379 Fragments arranged by size: 379 210 38 15 6 EarI CTCTTCn'nnn_ Cuts at: 0 570 648 Size: 570 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 115 367 648 Size: 115 252 281 Fragments arranged by size: 281 252 115 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 150 259 322 595 637 648 Size: 150 109 63 273 42 11 Fragments arranged by size: 273 150 109 63 42 11 EcoO109I rG'GnC_Cy Cuts at: 0 91 92 531 648 Size: 91 1 439 117 Fragments arranged by size: 439 117 91 1 FseI GG_CCGG'CC Cuts at: 0 44 648 Size: 44 604 FspI TGC'GCA Cuts at: 0 181 648 Size: 181 467 HaeII r_GCGC'y Cuts at: 0 69 314 648 Size: 69 245 334 Fragments arranged by size: 334 245 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 547 579 648 Size: 547 32 69 Fragments arranged by size: 547 69 32 KasI G'GCGC_C Cuts at: 0 65 310 648 Size: 65 245 338 Fragments arranged by size: 338 245 65 KpnI G_GTAC'C Cuts at: 0 300 648 Size: 300 348 MscI TGG'CCA Cuts at: 0 271 648 Size: 271 377 MspA1I CmG'CkG Cuts at: 0 170 450 456 648 Size: 170 280 6 192 Fragments arranged by size: 280 192 170 6 NaeI GCC'GGC Cuts at: 0 42 648 Size: 42 606 NarI GG'CG_CC Cuts at: 0 66 311 648 Size: 66 245 337 Fragments arranged by size: 337 245 66 NcoI C'CATG_G Cuts at: 0 509 648 Size: 509 139 NgoMIV G'CCGG_C Cuts at: 0 40 648 Size: 40 608 NspI r_CATG'y Cuts at: 0 561 648 Size: 561 87 PciI A'CATG_T Cuts at: 0 557 648 Size: 557 91 PmlI CAC'GTG Cuts at: 0 99 648 Size: 99 549 PpuMI rG'GwC_Cy Cuts at: 0 531 648 Size: 531 117 PspOMI G'GGCC_C Cuts at: 0 91 648 Size: 91 557 PvuII CAG'CTG Cuts at: 0 170 456 648 Size: 170 286 192 Fragments arranged by size: 286 192 170 RsrII CG'GwC_CG Cuts at: 0 263 648 Size: 263 385 ScaI AGT'ACT Cuts at: 0 343 648 Size: 343 305 SfoI GGC'GCC Cuts at: 0 67 312 648 Size: 67 245 336 Fragments arranged by size: 336 245 67 StyI C'CwwG_G Cuts at: 0 509 648 Size: 509 139 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 595 648 Size: 595 53 TatI w'GTAC_w Cuts at: 0 140 341 458 648 Size: 140 201 117 190 Fragments arranged by size: 201 190 140 117 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 29 148 180 648 Size: 29 119 32 468 Fragments arranged by size: 468 119 32 29 Tth111I GACn'n_nGTC Cuts at: 0 196 648 Size: 196 452 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 507 648 Size: 507 141 Enzymes that do cut: Acc65I AcuI AflIII AlwNI ApaI ApaLI BamHI BanI BanII BbeI BbsI BglI Bme1580I BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BsrBI BsrFI BsrGI BssHII BstAPI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I FseI FspI HaeII Hin4I KasI KpnI MscI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmlI PpuMI PspOMI PvuII RsrII ScaI SfoI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaBI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FspAI HincII HindIII HpaI MfeI MluI MmeI MslI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI SacI SacII SalI SanDI SapI SbfI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI XmaI XmnI ZraI