################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpmI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HinfI HpaI KpnI MaeI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SalI SapI ScaI SexAI SfiI SfuI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 868 GTCTAC GT^[AC][GT]AC AlwNI 898 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 225 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbrPI 667 CACGTG CAC^GTG BlpI 952 GCTGAGC GC^T[ACGT]AGC Bpu1102I 952 GCTGAGC GC^T[ACGT]AGC BsaAI 667 CACGTG [CT]AC^GT[GA] BsaI 732 GGTCTC GGTCTC(1/5) BseRI 157 CTCCTC GAGGAG(10/8) BsmAI 733 GTCTC GTCTC(1/5) BspHI 137 TCATGA T^CATGA BspMI 523 ACCTGC ACCTGC(4/8) BstAPI 347 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 519 GGTCACC G^GT[ACGT]ACC BstPI 519 GGTCACC G^GT[ACGT]ACC BstYI 797 AGATCC [GA]^GATC[CT] CelII 952 GCTGAGC GC^T[ACGT]AGC EcoNI 60 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 519 GGTCACC G^GT[ACGT]ACC KspI 558 CCGCGG CCGC^GG MflI 797 AGATCC [GA]^GATC[CT] MslI 996 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NruI 113 TCGCGA TCG^CGA PmaCI 667 CACGTG CAC^GTG PmlI 667 CACGTG CAC^GTG PstI 579 CTGCAG CTGCA^G RcaI 137 TCATGA T^CATGA SacII 558 CCGCGG CCGC^GG SbfI 578 CCTGCAGG CCTGCA^GG SgrAI 457 CGCCGGCG C[GA]^CCGG[CT]G SmaI 1014 CCCGGG CCC^GGG Sse8387I 578 CCTGCAGG CCTGCA^GG XcmI 257 CCAGCGCCGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 797 AGATCC [GA]^GATC[CT] XmaCI 1014 CCCGGG C^CCGGG XmaI 1014 CCCGGG C^CCGGG XmnI 225 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 455 GGCGCC G[GA]^CG[CT]C 689 GGCGCC AfaI 450 GTAC GT^AC 819 GTAC AluI 707 AGCT AG^CT 956 AGCT AlwI 798 GATCC GGATC(4/5) 767 GGATC Aor51HI 419 AGCGCT AGC^GCT 695 AGCGCT AvaI 814 CCCGAG C^[CT]CG[GA]G 1014 CCCGGG BalI 254 TGGCCA TGG^CCA 725 TGGCCA BanI 455 GGCGCC G^G[CT][GA]CC 689 GGCGCC BbeI 455 GGCGCC GGCGC^C 689 GGCGCC BglI 164 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 173 GCCTGGTGGGC BsaHI 455 GGCGCC G[GA]^CG[CT]C 689 GGCGCC BsoBI 814 CCCGAG C^[CT]CG[GA]G 1014 CCCGGG BsrBI 631 CCGCTC CCGCTC(-3/-3) 913 CCGCTC BsrI 452 ACTGG ACTGG(1/-1) 773 ACTGG EarI 277 CTCTTC CTCTTC(1/4) 781 CTCTTC Eco47III 419 AGCGCT AGC^GCT 695 AGCGCT EcoT14I 728 CCATGG C^C[AT][AT]GG 995 CCATGG Hin1I 455 GGCGCC G[GA]^CG[CT]C 689 GGCGCC HphI 521 TCACC GGTGA(8/7) 992 TCACC KasI 455 GGCGCC G^GCGCC 689 GGCGCC Ksp632I 277 CTCTTC CTCTTC(1/4) 781 CTCTTC MluNI 254 TGGCCA TGG^CCA 725 TGGCCA MscI 254 TGGCCA TGG^CCA 725 TGGCCA NaeI 402 GCCGGC GCC^GGC 458 GCCGGC NarI 455 GGCGCC GG^CGCC 689 GGCGCC NcoI 728 CCATGG C^CATGG 995 CCATGG NgoMIV 402 GCCGGC G^CCGGC 458 GCCGGC PpuMI 359 GGGTCCT [GA]G^G[AT]CC[CT] 503 GGGTCCT RsaI 450 GTAC GT^AC 819 GTAC SfcI 81 CTACAG C^T[GA][CT]AG 579 CTGCAG SfoI 455 GGCGCC GGC^GCC 689 GGCGCC StyI 728 CCATGG C^C[AT][AT]GG 995 CCATGG TaqI 104 TCGA T^CGA 770 TCGA TspRI 825 CACTG CA[GC]TG[ACGT][ACGT]^ 1006 CAGTG TthHB8I 104 TCGA T^CGA 770 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI ApaI AvaII BanII BbsI BbvI BcnI BmyI BpuAI BsaJI BsgI BsiEI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I