(Circular) (Six-base) MAPSORT of: pFN21AE9445.seq Check: 8340 from: 1 to: 5734 pFN21AE9445 5734 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2537 2537 Size: 5734 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2874 3077 1937 Size: 937 203 4594 Fragments arranged by size: 4594 937 203 Acc65I G'GTAC_C Cuts at: 3055 3055 Size: 5734 AclI AA'CG_TT Cuts at: 1563 3861 4234 1563 Size: 2298 373 3063 Fragments arranged by size: 3063 2298 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2269 3797 5181 5449 1632 Size: 637 1528 1384 268 1917 Fragments arranged by size: 1917 1528 1384 637 268 AfeI AGC'GCT Cuts at: 2426 2702 2426 Size: 276 5458 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 4633 5610 1203 Size: 3430 977 1327 Fragments arranged by size: 3430 1327 977 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 4463 4463 Size: 5734 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5734 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2222 2254 2222 Size: 32 5702 AlwNI CAG_nnn'CTG Cuts at: 2908 3477 5049 2908 Size: 569 1572 3593 Fragments arranged by size: 3593 1572 569 ApaI G_GGCC'C Cuts at: 2572 2689 2944 2572 Size: 117 255 5362 Fragments arranged by size: 5362 255 117 ApaLI G'TGCA_C Cuts at: 3791 4947 3791 Size: 1156 4578 ApoI r'AATT_y Cuts at: 1493 3043 3269 3421 5530 1493 Size: 1550 226 152 2109 1697 Fragments arranged by size: 2109 1697 1550 226 152 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 2819 3019 3059 1870 Size: 73 876 200 40 4545 Fragments arranged by size: 4545 876 200 73 40 BamHI G'GATC_C Cuts at: 3064 3431 3064 Size: 367 5367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2460 2694 3055 4510 618 Size: 325 217 627 673 234 361 1455 1842 Fragments arranged by size: 1842 1455 673 627 361 325 234 217 BanII G_rGCy'C Cuts at: 1391 2376 2572 2689 2944 3053 1391 Size: 985 196 117 255 109 4072 Fragments arranged by size: 4072 985 255 196 117 109 BbeI G_GCGC'C Cuts at: 2464 2698 2464 Size: 234 5500 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2737 2788 2923 928 Size: 971 838 51 135 3739 Fragments arranged by size: 3739 971 838 135 51 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3925 3959 1903 Size: 34 1988 34 3678 Fragments arranged by size: 3678 1988 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3626 4836 3626 Size: 1210 4524 BclI T'GATC_A Cuts at: 1548 1548 Size: 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2175 2184 4345 Size: 107 122 71 631 605 503 9 2161 Cuts at: 4345 136 Size: 1525 Fragments arranged by size: 2161 1525 631 605 503 122 107 71 9 BglII A'GATC_T Cuts at: 5729 5729 Size: 5734 BlpI GC'TnA_GC Cuts at: 2958 3151 2958 Size: 193 5541 Bme1580I G_kGCm'C Cuts at: 1790 2060 2333 2572 2689 2944 3795 4951 1790 Size: 270 273 239 117 255 851 1156 2573 Fragments arranged by size: 2573 1156 851 273 270 255 239 117 BmgBI CAC'GTC Cuts at: 1318 2722 1318 Size: 1404 4330 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3465 4423 442 Size: 416 53 83 399 24 2048 958 1753 Fragments arranged by size: 2048 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4394 1239 Size: 3155 2579 Bpu10I CC'TnA_GC Cuts at: 2066 2921 2066 Size: 855 4879 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3859 4724 5022 3859 Size: 865 298 4571 Fragments arranged by size: 4571 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2743 4397 882 Size: 617 1244 1654 2219 Fragments arranged by size: 2219 1654 1244 617 BsaAI yAC'GTr Cuts at: 493 1204 2674 3525 493 Size: 711 1470 851 2702 Fragments arranged by size: 2702 1470 851 711 BsaBI GATnn'nnATC Cuts at: 5728 5728 Size: 5734 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2461 2695 3541 3923 Size: 53 83 186 936 928 234 846 382 Cuts at: 3923 4558 275 Size: 635 1451 Fragments arranged by size: 1451 936 928 846 635 382 234 186 83 53 BsaWI w'CCGG_w Cuts at: 4167 4565 4839 4986 5397 4167 Size: 398 274 147 411 4504 Fragments arranged by size: 4504 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2222 2252 2996 3026 1178 Size: 30 165 30 819 30 744 30 3886 Fragments arranged by size: 3886 819 744 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 1186 Size: 967 4767 BseYI C'CCAG_C Cuts at: 2962 4937 5433 2962 Size: 1975 496 3263 Fragments arranged by size: 3263 1975 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 2519 2654 1163 Size: 573 501 282 135 4243 Fragments arranged by size: 4243 573 501 282 135 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2139 2412 2487 3184 3945 4973 Size: 275 186 138 273 75 697 761 1028 Cuts at: 4973 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 697 275 273 186 138 75 BsiHKAI G_wGCw'C Cuts at: 3795 3880 4951 3795 Size: 85 1071 4578 Fragments arranged by size: 4578 1071 85 BsmI GAATG_Cn' Cuts at: 3245 3338 3245 Size: 93 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2333 2376 2572 2689 2944 3053 Size: 399 270 273 43 196 117 255 109 Cuts at: 3053 3795 3880 4951 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 273 270 255 196 117 109 85 43 BspEI T'CCGG_A Cuts at: 5397 5397 Size: 5734 BspHI T'CATG_A Cuts at: 2142 3623 5353 2142 Size: 1481 1730 2523 Fragments arranged by size: 2523 1730 1481 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2537 3089 844 Size: 1693 552 3489 Fragments arranged by size: 3489 1693 552 BsrBI CCG'CTC Cuts at: 1394 1823 2638 2920 3621 1394 Size: 429 815 282 701 3507 Fragments arranged by size: 3507 815 701 429 282 BsrDI GCAATG_nn' Cuts at: 65 1248 4229 4403 65 Size: 1183 2981 174 1396 Fragments arranged by size: 2981 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2390 2407 2463 4378 4565 1700 Size: 110 580 17 56 1915 187 2869 Fragments arranged by size: 2869 1915 580 187 110 56 17 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5734 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5734 BssSI C'ACGA_G Cuts at: 3794 4806 3794 Size: 1012 4722 BstAPI GCAn_nnn'nTGC Cuts at: 794 2358 794 Size: 1564 4170 BstBI TT'CG_AA Cuts at: 4538 4538 Size: 5734 BstEII G'GTnAC_C Cuts at: 1184 2524 1184 Size: 1340 4394 BstYI r'GATC_y Cuts at: 1972 2802 3064 3431 3816 3833 5285 5371 5383 Size: 830 262 367 385 17 1452 86 12 Cuts at: 5383 5729 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 830 385 367 346 262 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2487 2563 2733 3000 513 Size: 552 939 483 76 170 267 3247 Fragments arranged by size: 3247 939 552 483 267 170 76 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2135 487 Size: 683 56 909 4086 Fragments arranged by size: 4086 909 683 56 BtsI GCAGTG_nn' Cuts at: 3017 3250 4044 4064 3017 Size: 233 794 20 4687 Fragments arranged by size: 4687 794 233 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5699 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4741 809 Size: 507 3425 1802 Fragments arranged by size: 3425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2259 2409 Size: 54 1425 50 275 18 306 123 150 Cuts at: 2409 2484 2730 2883 3181 4070 5699 8 Size: 75 246 153 298 889 1629 43 Fragments arranged by size: 1629 1425 889 306 298 275 246 153 150 123 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2136 2409 2484 3181 1537 Size: 275 324 273 75 697 4090 Fragments arranged by size: 4090 697 324 275 273 75 EarI CTCTTCn'nnn_ Cuts at: 1353 2288 2792 3664 1353 Size: 935 504 872 3423 Fragments arranged by size: 3423 935 872 504 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4321 4695 4841 387 Size: 1064 138 2732 374 146 1280 Fragments arranged by size: 2732 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2269 3797 4394 5181 5449 1239 Size: 393 637 1528 597 787 268 1524 Fragments arranged by size: 1528 1524 787 637 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2069 2069 Size: 5734 EcoO109I rG'GnC_Cy Cuts at: 2074 2365 2509 2568 2569 2941 2074 Size: 291 144 59 1 372 4867 Fragments arranged by size: 4867 372 291 144 59 1 EcoRI G'AATT_C Cuts at: 3043 3421 3043 Size: 378 5356 FspI TGC'GCA Cuts at: 4240 4240 Size: 5734 HaeII r_GCGC'y Cuts at: 1678 2268 2421 2428 2464 2479 2698 2704 2862 Size: 590 153 7 36 15 219 6 158 Cuts at: 2862 2958 4881 1678 Size: 96 1923 2531 Fragments arranged by size: 2531 1923 590 219 158 153 96 36 15 7 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2998 3030 4375 4407 4449 Size: 32 135 32 1426 32 1345 32 42 Cuts at: 4449 4481 1373 Size: 32 2626 Fragments arranged by size: 2626 1426 1345 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3078 3324 669 Size: 988 281 1140 246 3079 Fragments arranged by size: 3079 1140 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5734 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KasI G'GCGC_C Cuts at: 2460 2694 2460 Size: 234 5500 KpnI G_GTAC'C Cuts at: 3059 3059 Size: 5734 MfeI C'AATT_G Cuts at: 3333 3333 Size: 5734 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4848 5032 1305 Size: 3543 184 2007 Fragments arranged by size: 3543 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 2732 10 Size: 54 1425 343 429 471 3012 Fragments arranged by size: 3012 1425 471 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3005 3692 4051 4210 518 Size: 648 1839 687 359 159 2042 Fragments arranged by size: 2042 1839 687 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2565 2905 2938 3150 3827 4975 5220 1606 Size: 959 340 33 212 677 1148 245 2120 Fragments arranged by size: 2120 1148 959 677 340 245 212 33 NaeI GCC'GGC Cuts at: 2409 2465 2409 Size: 56 5678 NarI GG'CG_CC Cuts at: 2461 2695 2461 Size: 234 5500 NcoI C'CATG_G Cuts at: 513 1065 2004 2733 3000 513 Size: 552 939 729 267 3247 Fragments arranged by size: 3247 939 729 552 267 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NgoMIV G'CCGG_C Cuts at: 2407 2463 2407 Size: 56 5678 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5734 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NruI TCG'CGA Cuts at: 2120 2120 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 3092 4637 1132 Size: 97 1863 1545 2229 Fragments arranged by size: 2229 1863 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5734 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5734 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PmlI CAC'GTG Cuts at: 2674 2674 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2222 2254 3803 3835 5316 5348 2222 Size: 32 1549 32 1481 32 2608 Fragments arranged by size: 2608 1549 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2365 2509 2365 Size: 144 5590 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5734 PsiI TTA'TAA Cuts at: 3304 3304 Size: 5734 PspOMI G'GGCC_C Cuts at: 2568 2685 2940 2568 Size: 117 255 5362 Fragments arranged by size: 5362 255 117 PstI C_TGCA'G Cuts at: 830 2588 3086 830 Size: 1758 498 3478 Fragments arranged by size: 3478 1758 498 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 SacII CC_GC'GG Cuts at: 2566 2566 Size: 5734 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SbfI CC_TGCA'GG Cuts at: 2588 3086 2588 Size: 498 5236 ScaI AGT'ACT Cuts at: 3982 5694 3982 Size: 1712 4022 SfcI C'TryA_G Cuts at: 826 1045 2086 2584 3082 4217 4898 5089 826 Size: 219 1041 498 498 1135 681 191 1471 Fragments arranged by size: 1471 1135 1041 681 498 498 219 191 SfoI GGC'GCC Cuts at: 2462 2696 2462 Size: 234 5500 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 2463 1700 Size: 763 4971 SmaI CCC'GGG Cuts at: 1872 3021 3061 1872 Size: 1149 40 4545 Fragments arranged by size: 4545 1149 40 SmlI C'TyrA_G Cuts at: 820 1017 1943 3838 4739 5001 820 Size: 197 926 1895 901 262 1553 Fragments arranged by size: 1895 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 3092 1132 Size: 1960 3774 SspI AAT'ATT Cuts at: 5 52 3658 5 Size: 47 3606 2081 Fragments arranged by size: 3606 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5734 StyI C'CwwG_G Cuts at: 513 1065 2004 2733 3000 3173 513 Size: 552 939 729 267 173 3074 Fragments arranged by size: 3074 939 729 552 267 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2425 3761 3778 3931 4116 4561 325 Size: 1255 845 1336 17 153 185 445 1498 Fragments arranged by size: 1498 1336 1255 845 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3980 5692 96 Size: 274 80 33 51 1443 2003 1712 138 Fragments arranged by size: 2003 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2996 3028 5694 5726 2996 Size: 32 2666 32 3004 Fragments arranged by size: 3004 2666 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5734 XbaI T'CTAG_A Cuts at: 3070 3070 Size: 5734 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2269 2269 Size: 5734 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5734 XmaI C'CCGG_G Cuts at: 1870 3019 3059 1870 Size: 1149 40 4545 Fragments arranged by size: 4545 1149 40 XmnI GAAnn'nnTTC Cuts at: 2234 3863 2234 Size: 1629 4105 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI