(Linear) (Six-base) MAPSORT of: KIEE9445.seq Check: 3056 from: 1 to: 1029 KIEE9445.orf With 182 enzymes: SgfI * December 27, 2011 16:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 532 1029 Size: 532 497 AccI GT'mk_AC Cuts at: 0 869 1029 Size: 869 160 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 264 1029 Size: 264 765 AfeI AGC'GCT Cuts at: 0 421 697 1029 Size: 421 276 332 Fragments arranged by size: 421 332 276 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 217 249 1029 Size: 217 32 780 Fragments arranged by size: 780 217 32 AlwNI CAG_nnn'CTG Cuts at: 0 903 1029 Size: 903 126 ApaI G_GGCC'C Cuts at: 0 567 684 939 1029 Size: 567 117 255 90 Fragments arranged by size: 567 255 117 90 AvaI C'yCGr_G Cuts at: 0 814 1014 1029 Size: 814 200 15 Fragments arranged by size: 814 200 15 BanI G'GyrC_C Cuts at: 0 455 689 1029 Size: 455 234 340 Fragments arranged by size: 455 340 234 BanII G_rGCy'C Cuts at: 0 371 567 684 939 1029 Size: 371 196 117 255 90 Fragments arranged by size: 371 255 196 117 90 BbeI G_GCGC'C Cuts at: 0 459 693 1029 Size: 459 234 336 Fragments arranged by size: 459 336 234 BbsI GAAGACnn'nnnn_ Cuts at: 0 732 783 918 1029 Size: 732 51 135 111 Fragments arranged by size: 732 135 111 51 BglI GCCn_nnn'nGGC Cuts at: 0 170 179 1029 Size: 170 9 850 Fragments arranged by size: 850 170 9 BlpI GC'TnA_GC Cuts at: 0 953 1029 Size: 953 76 Bme1580I G_kGCm'C Cuts at: 0 55 328 567 684 939 1029 Size: 55 273 239 117 255 90 Fragments arranged by size: 273 255 239 117 90 55 BmgBI CAC'GTC Cuts at: 0 717 1029 Size: 717 312 Bpu10I CC'TnA_GC Cuts at: 0 61 916 1029 Size: 61 855 113 Fragments arranged by size: 855 113 61 BsaI GGTCTCn'nnnn_ Cuts at: 0 738 1029 Size: 738 291 BsaAI yAC'GTr Cuts at: 0 669 1029 Size: 669 360 BsaHI Gr'CG_yC Cuts at: 0 456 690 1029 Size: 456 234 339 Fragments arranged by size: 456 339 234 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 217 247 991 1021 1029 Size: 217 30 744 30 8 Fragments arranged by size: 744 217 30 30 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 148 1029 Size: 148 881 BseYI C'CCAG_C Cuts at: 0 957 1029 Size: 957 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 514 649 1029 Size: 232 282 135 380 Fragments arranged by size: 380 282 232 135 BsiEI CG_ry'CG Cuts at: 0 134 407 482 1029 Size: 134 273 75 547 Fragments arranged by size: 547 273 134 75 Bsp1286I G_dGCh'C Cuts at: 0 55 328 371 567 684 939 1029 Size: 55 273 43 196 117 255 90 Fragments arranged by size: 273 255 196 117 90 55 43 BspHI T'CATG_A Cuts at: 0 137 1029 Size: 137 892 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 532 1029 Size: 532 497 BsrBI CCG'CTC Cuts at: 0 633 915 1029 Size: 633 282 114 Fragments arranged by size: 633 282 114 BsrFI r'CCGG_y Cuts at: 0 385 402 458 1029 Size: 385 17 56 571 Fragments arranged by size: 571 385 56 17 BstAPI GCAn_nnn'nTGC Cuts at: 0 353 1029 Size: 353 676 BstEII G'GTnAC_C Cuts at: 0 519 1029 Size: 519 510 BstYI r'GATC_y Cuts at: 0 797 1029 Size: 797 232 BtgI C'CryG_G Cuts at: 0 482 558 728 995 1029 Size: 482 76 170 267 34 Fragments arranged by size: 482 267 170 76 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 130 1029 Size: 130 899 BtsI GCAGTG_nn' Cuts at: 0 1012 1029 Size: 1012 17 EaeI y'GGCC_r Cuts at: 0 131 254 404 479 725 878 1029 Size: 131 123 150 75 246 153 151 Fragments arranged by size: 246 153 151 150 131 123 75 EagI C'GGCC_G Cuts at: 0 131 404 479 1029 Size: 131 273 75 550 Fragments arranged by size: 550 273 131 75 EarI CTCTTCn'nnn_ Cuts at: 0 283 787 1029 Size: 283 504 242 Fragments arranged by size: 504 283 242 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 264 1029 Size: 264 765 EcoNI CCTnn'n_nnAGG Cuts at: 0 64 1029 Size: 64 965 EcoO109I rG'GnC_Cy Cuts at: 0 69 360 504 563 564 936 1029 Size: 69 291 144 59 1 372 93 Fragments arranged by size: 372 291 144 93 69 59 1 HaeII r_GCGC'y Cuts at: 0 263 416 423 459 474 693 699 857 Size: 263 153 7 36 15 219 6 158 Cuts at: 857 953 1029 Size: 96 76 Fragments arranged by size: 263 219 158 153 96 76 36 15 7 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 993 1025 1029 Size: 993 32 4 Fragments arranged by size: 993 32 4 KasI G'GCGC_C Cuts at: 0 455 689 1029 Size: 455 234 340 Fragments arranged by size: 455 340 234 MscI TGG'CCA Cuts at: 0 256 727 1029 Size: 256 471 302 Fragments arranged by size: 471 302 256 MslI CAynn'nnrTG Cuts at: 0 1000 1029 Size: 1000 29 MspA1I CmG'CkG Cuts at: 0 560 900 933 1029 Size: 560 340 33 96 Fragments arranged by size: 560 340 96 33 NaeI GCC'GGC Cuts at: 0 404 460 1029 Size: 404 56 569 Fragments arranged by size: 569 404 56 NarI GG'CG_CC Cuts at: 0 456 690 1029 Size: 456 234 339 Fragments arranged by size: 456 339 234 NcoI C'CATG_G Cuts at: 0 728 995 1029 Size: 728 267 34 Fragments arranged by size: 728 267 34 NgoMIV G'CCGG_C Cuts at: 0 402 458 1029 Size: 402 56 571 Fragments arranged by size: 571 402 56 NruI TCG'CGA Cuts at: 0 115 1029 Size: 115 914 PmlI CAC'GTG Cuts at: 0 669 1029 Size: 669 360 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 217 249 1029 Size: 217 32 780 Fragments arranged by size: 780 217 32 PpuMI rG'GwC_Cy Cuts at: 0 360 504 1029 Size: 360 144 525 Fragments arranged by size: 525 360 144 PspOMI G'GGCC_C Cuts at: 0 563 680 935 1029 Size: 563 117 255 94 Fragments arranged by size: 563 255 117 94 PstI C_TGCA'G Cuts at: 0 583 1029 Size: 583 446 SacII CC_GC'GG Cuts at: 0 561 1029 Size: 561 468 SbfI CC_TGCA'GG Cuts at: 0 583 1029 Size: 583 446 SfcI C'TryA_G Cuts at: 0 81 579 1029 Size: 81 498 450 Fragments arranged by size: 498 450 81 SfoI GGC'GCC Cuts at: 0 457 691 1029 Size: 457 234 338 Fragments arranged by size: 457 338 234 SgrAI Cr'CCGG_yG Cuts at: 0 458 1029 Size: 458 571 SmaI CCC'GGG Cuts at: 0 1016 1029 Size: 1016 13 StyI C'CwwG_G Cuts at: 0 728 995 1029 Size: 728 267 34 Fragments arranged by size: 728 267 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 420 1029 Size: 420 609 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 991 1023 1029 Size: 991 32 6 Fragments arranged by size: 991 32 6 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 264 1029 Size: 264 765 XmaI C'CCGG_G Cuts at: 0 1014 1029 Size: 1014 15 XmnI GAAnn'nnTTC Cuts at: 0 229 1029 Size: 229 800 Enzymes that do cut: AarI AccI AcuI AfeI AloI AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstEII BstYI BtgI BtgZI BtsI EaeI EagI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI PmlI PpiI PpuMI PspOMI PstI SacII SbfI SfcI SfoI SgrAI SmaI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI BpmI BpuEI BsaBI BsaWI BsiHKAI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI ZraI