################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AccIII AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BfrI BglII BlnI BseAI BsmI BspDI BspEI Bst1107I BstBI BstEII BstPI BstZ17I ClaI DraI DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI MfeI MluI MroI MseI MunI NdeI NheI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI SexAI SfiI SfuI SnaBI SpeI SspI SwaI Tru9I Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 3236 GGTACC G^GTACC Alw44I 1711 GTGCAC G^TGCAC AocI 1927 CCTGAGG CC^T[ACGT]AGG Aor51HI 4238 AGCGCT AGC^GCT ApaLI 1711 GTGCAC G^TGCAC Asp718I 3236 GGTACC G^GTACC BamHI 2304 GGATCC G^GATCC BciVI 3818 GTATCC GTATCC(6/5) BclI 1586 TGATCA T^GATCA BsaBI 2667 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 126 CGTACG C^GTACG Bsp1407I 4529 TGTACA T^GTACA BspHI 2108 TCATGA T^CATGA BsrDI 1125 CATTGC GCAATG(2/0) BsrGI 4529 TGTACA T^GTACA BssHII 3914 GCGCGC G^CGCGC Bsu36I 1927 CCTGAGG CC^T[ACGT]AGG Eco47III 4238 AGCGCT AGC^GCT Eco81I 1927 CCTGAGG CC^T[ACGT]AGG FbaI 1586 TGATCA T^GATCA KpnI 3236 GGTACC GGTAC^C KspI 86 CCGCGG CCGC^GG MamI 2667 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 142 GCCGGC GCC^GGC NgoMIV 142 GCCGGC G^CCGGC NotI 193 GCGGCCGC GC^GGCCGC PaeR7I 1978 CTCGAG C^TCGAG RcaI 2108 TCATGA T^CATGA SacII 86 CCGCGG CCGC^GG SalI 1267 GTCGAC G^TCGAC SapI 432 GCTCTTC GCTCTTC(1/4) SbfI 1533 CCTGCAGG CCTGCA^GG ScaI 4682 AGTACT AGT^ACT SgrAI 141 CGCCGGCG C[GA]^CCGG[CT]G SmlI 1978 CTCGAG C^T[CT][GA]AG SphI 4354 GCATGC GCATG^C Sse8387I 1533 CCTGCAGG CCTGCA^GG SspBI 4529 TGTACA T^GTACA Tsp509I 4306 AATT ^AATT XhoI 1978 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AatII 1735 GACGTC GACGT^C 2077 GACGTC ApaI 4162 GGGCCC GGGCC^C 4258 GGGCCC BbeI 2987 GGCGCC GGCGC^C 4608 GGCGCC BbrPI 2131 CACGTG CAC^GTG 4294 CACGTG BbvCI 2030 CCTCAGC CCTCAGC(-5/-2) 3084 CCTCAGC BfaI 1147 CTAG C^TAG 2621 CTAG BsaAI 2131 CACGTG [CT]AC^GT[GA] 4294 CACGTG BsaWI 4022 TCCGGT [AT]^CCGG[AT] 4421 ACCGGA BsmBI 514 GAGACG CGTCTC(1/5) 1559 GAGACG BspLU11I 491 ACATGT A^CATGT 4541 ACATGT BsrFI 142 GCCGGC [GA]^CCGG[CT] 3325 ACCGGC BstAPI 3741 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4349 GCATCGCATGC Cfr10I 142 GCCGGC [GA]^CCGG[CT] 3325 ACCGGC CpoI 18 CGGACCG CG^G[AT]CCG 1310 CGGACCG EagI 194 CGGCCG C^GGCCG 2625 CGGCCG EclXI 194 CGGCCG C^GGCCG 2625 CGGCCG Eco52I 194 CGGCCG C^GGCCG 2625 CGGCCG KasI 2987 GGCGCC G^GCGCC 4608 GGCGCC MaeI 1147 CTAG C^TAG 2621 CTAG NarI 2987 GGCGCC GG^CGCC 4608 GGCGCC NcoI 842 CCATGG C^CATGG 3170 CCATGG PmaCI 2131 CACGTG CAC^GTG 4294 CACGTG PmlI 2131 CACGTG CAC^GTG 4294 CACGTG PshAI 1224 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2073 GACAGACGTC RsrII 18 CGGACCG CG^G[AT]CCG 1310 CGGACCG SfoI 2987 GGCGCC GGC^GCC 4608 GGCGCC SmaI 3823 CCCGGG CCC^GGG 4317 CCCGGG StuI 1931 AGGCCT AGG^CCT 2027 AGGCCT TfiI 2222 GAATC G^A[AT]TC 2589 GAATC XmaCI 3823 CCCGGG C^CCGGG 4317 CCCGGG XmaI 3823 CCCGGG C^CCGGG 4317 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BcnI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XhoII