################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BbeI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsaI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraIII Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HpaI KasI KspI MamI MfeI MluI MseI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PstI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 796 GACGTC GACGT^C Acc65I 413 GGTACC G^GTACC AccII 939 CGCG CG^CG AccIII 905 TCCGGA T^CCGGA AcyI 796 GACGTC G[GA]^CG[CT]C AflIII 190 ACACGT A^C[GA][CT]GT ApaI 1319 GGGCCC GGGCC^C Asp718I 413 GGTACC G^GTACC BanII 1319 GGGCCC G[GA]GC[CT]^C BbrPI 634 CACGTG CAC^GTG BglI 706 GCCCGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 634 CACGTG [CT]AC^GT[GA] BsaHI 796 GACGTC G[GA]^CG[CT]C BsaWI 905 TCCGGA [AT]^CCGG[AT] BseAI 905 TCCGGA T^CCGGA BsgI 1244 CTGCAC GTGCAG(16/14) BspEI 905 TCCGGA T^CCGGA BsrBI 958 GAGCGG CCGCTC(-3/-3) BstAPI 1246 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 939 CGCG CG^CG DsaI 11 CCATGG C^C[GA][CT]GG EagI 961 CGGCCG C^GGCCG EclXI 961 CGGCCG C^GGCCG Eco52I 961 CGGCCG C^GGCCG EcoNI 1309 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Hin1I 796 GACGTC G[GA]^CG[CT]C HindIII 1283 AAGCTT A^AGCTT KpnI 413 GGTACC GGTAC^C MaeIII 1047 GTCAC ^GT[ACGT]AC MroI 905 TCCGGA T^CCGGA MslI 6 CACTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 939 CGCG CG^CG NaeI 160 GCCGGC GCC^GGC NcoI 11 CCATGG C^CATGG NgoMIV 160 GCCGGC G^CCGGC PflMI 133 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 70 GACTC GAGTC(4/5) PmaCI 634 CACGTG CAC^GTG PmlI 634 CACGTG CAC^GTG PvuI 1099 CGATCG CGAT^CG SexAI 533 ACCTGGT A^CC[AT]GGT SgrAI 456 CGCCGGTG C[GA]^CCGG[CT]G SmaI 1392 CCCGGG CCC^GGG SmlI 1324 CTTGAG C^T[CT][GA]AG TaqI 380 TCGA T^CGA Tsp45I 1047 GTCAC ^GT[GC]AC TthHB8I 380 TCGA T^CGA Van91I 133 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1392 CCCGGG C^CCGGG XmaI 1392 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 799 GTCTAC GT^[AC][GT]AC 832 GTCTAC AfaI 414 GTAC GT^AC 528 GTAC AlwNI 1199 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG 1269 CAGTCCCTG AocI 744 CCTGAGG CC^T[ACGT]AGG 1072 CCTCAGG AspHI 637 GTGCTC G[AT]GC[AT]^C 925 GTGCTC AspI 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 532 GACCTGGTC AvaI 508 CCCGAG C^[CT]CG[GA]G 1392 CCCGGG BbsI 100 GTCTTC GAAGAC(2/6) 502 GTCTTC BfaI 265 CTAG C^TAG 1000 CTAG BlpI 210 GCTCAGC GC^T[ACGT]AGC 1114 GCTCAGC BpmI 1090 CTCCAG CTGGAG(16/14) 586 CTGGAG Bpu1102I 210 GCTCAGC GC^T[ACGT]AGC 1114 GCTCAGC BpuAI 100 GTCTTC GAAGAC(2/6) 502 GTCTTC BsiHKAI 637 GTGCTC G[AT]GC[AT]^C 925 GTGCTC BsoBI 508 CCCGAG C^[CT]CG[GA]G 1392 CCCGGG BsrFI 160 GCCGGC [GA]^CCGG[CT] 457 GCCGGT Bsu36I 744 CCTGAGG CC^T[ACGT]AGG 1072 CCTCAGG CelII 210 GCTCAGC GC^T[ACGT]AGC 1114 GCTCAGC Cfr10I 160 GCCGGC [GA]^CCGG[CT] 457 GCCGGT DrdI 720 GACCATTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1225 GACACTAACGTC EarI 355 CTCTTC CTCTTC(1/4) 481 CTCTTC Eco81I 744 CCTGAGG CC^T[ACGT]AGG 1072 CCTCAGG HaeII 198 GGCGCT [GA]GCGC^[CT] 396 GGCGCT HphI 1077 GGTGA GGTGA(8/7) 641 TCACC Ksp632I 355 CTCTTC CTCTTC(1/4) 481 CTCTTC MaeI 265 CTAG C^TAG 1000 CTAG PflFI 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 532 GACCTGGTC PpuMI 590 AGGTCCT [GA]G^G[AT]CC[CT] 1328 AGGACCC RsaI 414 GTAC GT^AC 528 GTAC SfaNI 582 GATGC GCATC(5/9) 1305 GCATC TfiI 1003 GATTC G^A[AT]TC 1201 GATTC Tth111I 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 532 GACCTGGTC XcmI 137 CCAATGGGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 168 CCAGGCCCGGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AluI AlwI AvaII BalI BamHI BanI BbvCI BbvI BcnI BmyI BsaJI BseRI BsiEI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI StyI TseI TspRI XhoII