(Circular) (Six-base) MAPSORT of: pFN21AE9406.seq Check: 7129 from: 1 to: 6115 pFN21AE9406 6115 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2678 3262 2678 Size: 584 5531 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2805 3925 278 Size: 53 83 186 936 1269 1120 2468 Fragments arranged by size: 2468 1269 1120 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2805 2838 3458 1937 Size: 868 33 620 4594 Fragments arranged by size: 4594 868 620 33 Acc65I G'GTAC_C Cuts at: 2418 3436 2418 Size: 1018 5097 AclI AA'CG_TT Cuts at: 1563 4242 4615 1563 Size: 2679 373 3063 Fragments arranged by size: 3063 2679 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2473 3378 4178 5562 5830 1632 Size: 841 905 800 1384 268 1917 Fragments arranged by size: 1917 1384 905 841 800 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5918 AflIII A'CryG_T Cuts at: 1203 2195 5014 5991 1203 Size: 992 2819 977 1327 Fragments arranged by size: 2819 1327 992 977 AgeI A'CCGG_T Cuts at: 4946 4946 Size: 6115 AhdI GACnn_n'nnGTC Cuts at: 4844 4844 Size: 6115 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2971 1184 Size: 1787 4328 AlwNI CAG_nnn'CTG Cuts at: 3209 3279 3858 5430 3209 Size: 70 579 1572 3894 Fragments arranged by size: 3894 1572 579 70 ApaI G_GGCC'C Cuts at: 3328 3328 Size: 6115 ApaLI G'TGCA_C Cuts at: 4172 5328 4172 Size: 1156 4959 ApoI r'AATT_y Cuts at: 1493 3424 3650 3802 5911 1493 Size: 1931 226 152 2109 1697 Fragments arranged by size: 2109 1931 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4669 160 Size: 872 3637 1606 Fragments arranged by size: 3637 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 AvaI C'yCGr_G Cuts at: 1870 1943 2513 3397 3440 1870 Size: 73 570 884 43 4545 Fragments arranged by size: 4545 884 570 73 43 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2402 2435 2402 Size: 33 6082 BamHI G'GATC_C Cuts at: 3061 3109 3157 3445 3812 3061 Size: 48 48 288 367 5364 Fragments arranged by size: 5364 367 288 48 48 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2418 2427 3436 4891 Size: 325 217 627 246 385 9 1009 1455 Cuts at: 4891 618 Size: 1842 Fragments arranged by size: 1842 1455 1009 627 385 325 246 217 9 BanII G_rGCy'C Cuts at: 1391 3328 3434 1391 Size: 1937 106 4072 Fragments arranged by size: 4072 1937 106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2098 2500 928 Size: 971 199 402 4543 Fragments arranged by size: 4543 971 402 199 BbvCI CC'TCA_GC Cuts at: 2378 2582 2873 2378 Size: 204 291 5620 Fragments arranged by size: 5620 291 204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4306 4340 1903 Size: 34 2369 34 3678 Fragments arranged by size: 3678 2369 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4007 5217 4007 Size: 1210 4905 BclI T'GATC_A Cuts at: 1548 1548 Size: 6115 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2717 4726 136 Size: 107 122 71 631 605 1045 2009 1525 Fragments arranged by size: 2009 1525 1045 631 605 122 107 71 BglII A'GATC_T Cuts at: 6110 6110 Size: 6115 BlpI GC'TnA_GC Cuts at: 2216 3120 3532 2216 Size: 904 412 4799 Fragments arranged by size: 4799 904 412 Bme1580I G_kGCm'C Cuts at: 1790 2411 2430 2499 3273 3328 4176 5332 1790 Size: 621 19 69 774 55 848 1156 2573 Fragments arranged by size: 2573 1156 848 774 621 69 55 19 BmgBI CAC'GTC Cuts at: 1318 2198 1318 Size: 880 5235 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2429 3043 3267 Size: 416 53 83 399 24 1012 614 224 Cuts at: 3267 3846 4804 442 Size: 579 958 1753 Fragments arranged by size: 1753 1012 958 614 579 416 399 224 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6115 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2612 3080 4775 1239 Size: 1373 468 1695 2579 Fragments arranged by size: 2579 1695 1373 468 Bpu10I CC'TnA_GC Cuts at: 2378 2582 2873 2378 Size: 204 291 5620 Fragments arranged by size: 5620 291 204 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3350 4240 5105 5403 3350 Size: 890 865 298 4062 Fragments arranged by size: 4062 890 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4778 882 Size: 617 3279 2219 Fragments arranged by size: 3279 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2641 3906 493 Size: 711 1437 1265 2702 Fragments arranged by size: 2702 1437 1265 711 BsaBI GATnn'nnATC Cuts at: 6109 6109 Size: 6115 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2802 3922 4304 4939 Size: 53 83 186 936 1269 1120 382 635 Cuts at: 4939 275 Size: 1451 Fragments arranged by size: 1451 1269 1120 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2910 4548 4946 5220 5367 5778 2910 Size: 1638 398 274 147 411 3247 Fragments arranged by size: 3247 1638 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5890 Fragments arranged by size: 5890 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2341 2585 2975 1186 Size: 1155 244 390 4326 Fragments arranged by size: 4326 1155 390 244 BseYI C'CCAG_C Cuts at: 2094 2299 2772 3000 3072 3144 3168 3192 3216 Size: 205 473 228 72 72 24 24 24 Cuts at: 3216 3337 3371 5318 5814 2094 Size: 121 34 1947 496 2395 Fragments arranged by size: 2395 1947 496 473 228 205 121 72 72 34 24 24 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3234 1163 Size: 573 1498 4044 Fragments arranged by size: 4044 1498 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2389 2969 3107 3565 4326 5354 Size: 275 186 388 580 138 458 761 1028 Cuts at: 5354 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 580 458 388 275 186 138 BsiHKAI G_wGCw'C Cuts at: 2646 2934 4176 4261 5332 2646 Size: 288 1242 85 1071 3429 Fragments arranged by size: 3429 1242 1071 288 85 BsmI GAATG_Cn' Cuts at: 3626 3719 3626 Size: 93 6022 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2411 2430 2499 2646 2934 3273 3328 Size: 399 621 19 69 147 288 339 55 Cuts at: 3328 3434 4176 4261 5332 1391 Size: 106 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 621 399 339 288 147 106 85 69 55 19 BspEI T'CCGG_A Cuts at: 2910 5778 2910 Size: 2868 3247 BspHI T'CATG_A Cuts at: 4004 5734 4004 Size: 1730 4385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2678 2826 3262 3470 844 Size: 1834 148 436 208 3489 Fragments arranged by size: 3489 1834 436 208 148 BsrBI CCG'CTC Cuts at: 1394 1823 2965 4002 1394 Size: 429 1142 1037 3507 Fragments arranged by size: 3507 1142 1037 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4610 4784 65 Size: 1183 3362 174 1396 Fragments arranged by size: 3362 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2165 2462 4759 4946 1700 Size: 110 355 297 2297 187 2869 Fragments arranged by size: 2869 2297 355 297 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6115 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6115 BssSI C'ACGA_G Cuts at: 4175 5187 4175 Size: 1012 5103 BstAPI GCAn_nnn'nTGC Cuts at: 794 3257 794 Size: 2463 3652 BstBI TT'CG_AA Cuts at: 4919 4919 Size: 6115 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6115 BstYI r'GATC_y Cuts at: 1972 2265 2274 3061 3109 3157 3445 3812 4197 Size: 293 9 787 48 48 288 367 385 Cuts at: 4197 4214 5666 5752 5764 6110 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 787 385 367 346 293 288 86 48 48 17 12 9 Bsu36I CC'TnA_GG Cuts at: 2750 3078 2750 Size: 328 5787 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 3411 513 Size: 552 939 12 1395 3217 Fragments arranged by size: 3217 1395 939 552 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5376 Fragments arranged by size: 5376 683 56 BtsI GCAGTG_nn' Cuts at: 3631 4425 4445 3631 Size: 794 20 5301 Fragments arranged by size: 5301 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6115 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6080 DraI TTT'AAA Cuts at: 3419 3419 Size: 6115 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2731 3236 5122 809 Size: 507 1415 505 1886 1802 Fragments arranged by size: 1886 1802 1415 507 505 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2041 2163 2294 Size: 54 1425 50 275 18 211 122 131 Cuts at: 2294 2370 2478 2718 2895 2966 3562 4451 6080 Size: 76 108 240 177 71 596 889 1629 Cuts at: 6080 8 Size: 43 Fragments arranged by size: 1629 1425 889 596 275 240 211 177 131 122 108 76 71 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2966 3562 1537 Size: 275 1154 596 4090 Fragments arranged by size: 4090 1154 596 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2366 2492 4045 1353 Size: 1013 126 1553 3423 Fragments arranged by size: 3423 1553 1013 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2429 4702 5076 5222 387 Size: 1064 138 840 2273 374 146 1280 Fragments arranged by size: 2273 1280 1064 840 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2473 2612 3080 3378 4178 4775 5562 Size: 393 841 139 468 298 800 597 787 Cuts at: 5562 5830 1239 Size: 268 1524 Fragments arranged by size: 1524 841 800 787 597 468 393 298 268 139 EcoNI CCTnn'n_nnAGG Cuts at: 3318 3318 Size: 6115 EcoO109I rG'GnC_Cy Cuts at: 2049 2596 2830 3324 3325 3334 3407 2049 Size: 547 234 494 1 9 73 4757 Fragments arranged by size: 4757 547 494 234 73 9 1 EcoRI G'AATT_C Cuts at: 3424 3802 3424 Size: 378 5737 FspI TGC'GCA Cuts at: 4621 4621 Size: 6115 HaeII r_GCGC'y Cuts at: 1678 2207 2405 5262 1678 Size: 529 198 2857 2531 Fragments arranged by size: 2857 2531 529 198 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2561 2593 2792 Size: 32 135 32 488 32 469 32 199 Cuts at: 2792 2824 3035 3067 4756 4788 4830 4862 1373 Size: 32 211 32 1689 32 42 32 2626 Fragments arranged by size: 2626 1689 488 469 211 199 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3459 3705 669 Size: 988 281 1521 246 3079 Fragments arranged by size: 3079 1521 988 281 246 HindIII A'AGCT_T Cuts at: 748 3288 748 Size: 2540 3575 HpaI GTT'AAC Cuts at: 3705 3705 Size: 6115 KpnI G_GTAC'C Cuts at: 2422 3440 2422 Size: 1018 5097 MfeI C'AATT_G Cuts at: 3714 3714 Size: 6115 MluI A'CGCG_T Cuts at: 5991 5991 Size: 6115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2868 3044 3135 5229 5413 1305 Size: 1563 176 91 2094 184 2007 Fragments arranged by size: 2094 2007 1563 184 176 91 MscI TGG'CCA Cuts at: 10 64 1489 1832 2043 2480 2720 2897 10 Size: 54 1425 343 211 437 240 177 3228 Fragments arranged by size: 3228 1425 437 343 240 211 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2015 4073 4432 4591 518 Size: 648 849 2058 359 159 2042 Fragments arranged by size: 2058 2042 849 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2255 2299 2309 2391 2767 2776 2868 3130 Size: 649 44 10 82 376 9 92 262 Cuts at: 3130 3178 3531 4208 5356 5601 1606 Size: 48 353 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 649 376 353 262 245 92 82 48 44 10 9 NaeI GCC'GGC Cuts at: 2167 2167 Size: 6115 NcoI C'CATG_G Cuts at: 513 1065 2004 2016 513 Size: 552 939 12 4612 Fragments arranged by size: 4612 939 552 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 6115 NgoMIV G'CCGG_C Cuts at: 2165 2165 Size: 6115 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6115 NotI GC'GGCC_GC Cuts at: 3562 3562 Size: 6115 NspI r_CATG'y Cuts at: 1132 1229 3473 5018 1132 Size: 97 2244 1545 2229 Fragments arranged by size: 2244 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2423 3320 1348 Size: 1075 897 4143 Fragments arranged by size: 4143 1075 897 PciI A'CATG_T Cuts at: 5014 5014 Size: 6115 PflMI CCAn_nnn'nTGG Cuts at: 1108 2144 1108 Size: 1036 5079 PmeI GTTT'AAAC Cuts at: 3419 3419 Size: 6115 PmlI CAC'GTG Cuts at: 2641 2641 Size: 6115 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4184 4216 5697 5729 4184 Size: 32 1481 32 4570 Fragments arranged by size: 4570 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2596 3334 2596 Size: 738 5377 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6115 PsiI TTA'TAA Cuts at: 3685 3685 Size: 6115 PspOMI G'GGCC_C Cuts at: 3324 3324 Size: 6115 PstI C_TGCA'G Cuts at: 830 3467 830 Size: 2637 3478 PvuI CG_AT'CG Cuts at: 2001 3107 2001 Size: 1106 5009 PvuII CAG'CTG Cuts at: 2309 2767 2776 2868 3130 3178 2309 Size: 458 9 92 262 48 5246 Fragments arranged by size: 5246 458 262 92 48 9 SalI G'TCGA_C Cuts at: 1936 3457 1936 Size: 1521 4594 SbfI CC_TGCA'GG Cuts at: 3467 3467 Size: 6115 ScaI AGT'ACT Cuts at: 4363 6075 4363 Size: 1712 4403 SexAI A'CCwGG_T Cuts at: 2538 2538 Size: 6115 SfcI C'TryA_G Cuts at: 826 1045 3463 4598 5279 5470 826 Size: 219 2418 1135 681 191 1471 Fragments arranged by size: 2418 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 SgrAI Cr'CCGG_yG Cuts at: 1700 2462 1700 Size: 762 5353 SmaI CCC'GGG Cuts at: 1872 3399 3442 1872 Size: 1527 43 4545 Fragments arranged by size: 4545 1527 43 SmlI C'TyrA_G Cuts at: 820 1017 1943 3329 4219 5120 5382 820 Size: 197 926 1386 890 901 262 1553 Fragments arranged by size: 1553 1386 926 901 890 262 197 SnaBI TAC'GTA Cuts at: 493 3906 493 Size: 3413 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6115 SphI G_CATG'C Cuts at: 1132 3473 1132 Size: 2341 3774 SspI AAT'ATT Cuts at: 5 52 4039 5 Size: 47 3987 2081 Fragments arranged by size: 3987 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6115 StyI C'CwwG_G Cuts at: 513 1065 2004 2016 2086 2128 3554 513 Size: 552 939 12 70 42 1426 3074 Fragments arranged by size: 3074 1426 939 552 70 42 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2021 2461 2663 4142 4159 4312 4497 Size: 1255 441 440 202 1479 17 153 185 Cuts at: 4497 4942 325 Size: 445 1498 Fragments arranged by size: 1498 1479 1255 445 441 440 202 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4361 6073 96 Size: 274 80 33 51 1443 2384 1712 138 Fragments arranged by size: 2384 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6075 6107 6075 Size: 32 6083 Tth111I GACn'n_nGTC Cuts at: 1316 2102 2540 1316 Size: 786 438 4891 Fragments arranged by size: 4891 786 438 XbaI T'CTAG_A Cuts at: 3451 3451 Size: 6115 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2149 2180 2149 Size: 31 6084 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6115 XmaI C'CCGG_G Cuts at: 1870 3397 3440 1870 Size: 1527 43 4545 Fragments arranged by size: 4545 1527 43 XmnI GAAnn'nnTTC Cuts at: 4244 4244 Size: 6115 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2803 3923 276 Size: 53 83 186 936 1269 1120 2468 Fragments arranged by size: 2468 1269 1120 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI