(Circular) (Six-base) MAPSORT of: pFN21AB8478.seq Check: 4833 from: 1 to: 7246 pFN21AB8478 7246 bp With 182 enzymes: SgfI * March 28, 2012 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3050 3342 3382 4462 3050 Size: 292 40 1080 5834 Fragments arranged by size: 5834 1080 292 40 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2919 3658 5056 278 Size: 53 83 186 936 1383 739 1398 2468 Fragments arranged by size: 2468 1398 1383 936 739 186 83 53 AccI GT'mk_AC Cuts at: 1937 4589 1937 Size: 2652 4594 Acc65I G'GTAC_C Cuts at: 3774 4567 3774 Size: 793 6453 AclI AA'CG_TT Cuts at: 1563 5373 5746 1563 Size: 3810 373 3063 Fragments arranged by size: 3810 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2813 5309 6693 6961 1632 Size: 1181 2496 1384 268 1917 Fragments arranged by size: 2496 1917 1384 1181 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7049 AflIII A'CryG_T Cuts at: 1203 6145 7122 1203 Size: 4942 977 1327 Fragments arranged by size: 4942 1327 977 AgeI A'CCGG_T Cuts at: 6077 6077 Size: 7246 AhdI GACnn_n'nnGTC Cuts at: 5975 5975 Size: 7246 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7246 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3530 3971 1184 Size: 2346 441 4459 Fragments arranged by size: 4459 2346 441 AlwNI CAG_nnn'CTG Cuts at: 2353 4989 6561 2353 Size: 2636 1572 3038 Fragments arranged by size: 3038 2636 1572 ApaI G_GGCC'C Cuts at: 2604 4478 2604 Size: 1874 5372 ApaLI G'TGCA_C Cuts at: 5303 6459 5303 Size: 1156 6090 ApoI r'AATT_y Cuts at: 1493 4555 4781 4933 7042 1493 Size: 3062 226 152 2109 1697 Fragments arranged by size: 3062 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5800 160 Size: 872 4768 1606 Fragments arranged by size: 4768 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7246 AvaI C'yCGr_G Cuts at: 1870 1943 2220 2307 2817 3047 3863 4571 1870 Size: 73 277 87 510 230 816 708 4545 Fragments arranged by size: 4545 816 708 510 277 230 87 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4138 4171 4138 Size: 33 7213 BamHI G'GATC_C Cuts at: 4576 4943 4576 Size: 367 6879 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2707 2855 2962 3774 3947 Size: 325 217 627 920 148 107 812 173 Cuts at: 3947 4256 4265 4511 4528 4567 6022 618 Size: 309 9 246 17 39 1455 1842 Fragments arranged by size: 1842 1455 920 812 627 325 309 246 217 173 148 107 39 17 9 BanII G_rGCy'C Cuts at: 1391 2013 2039 2166 2565 2604 2617 3054 3864 Size: 622 26 127 399 39 13 437 810 Cuts at: 3864 3873 4187 4206 4478 4565 1391 Size: 9 314 19 272 87 4072 Fragments arranged by size: 4072 810 622 437 399 314 272 127 87 39 26 19 13 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3544 928 Size: 971 1645 4630 Fragments arranged by size: 4630 1645 971 BbvCI CC'TCA_GC Cuts at: 2606 2606 Size: 7246 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3472 3506 5437 5471 1903 Size: 34 1535 34 1931 34 3678 Fragments arranged by size: 3678 1931 1535 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4166 5138 6348 4166 Size: 972 1210 5064 Fragments arranged by size: 5064 1210 972 BclI T'GATC_A Cuts at: 1548 1548 Size: 7246 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2170 2280 2516 Size: 107 122 71 631 605 498 110 236 Cuts at: 2516 2543 2870 3877 5857 136 Size: 27 327 1007 1980 1525 Fragments arranged by size: 1980 1525 1007 631 605 498 327 236 122 110 107 71 27 BglII A'GATC_T Cuts at: 7241 7241 Size: 7246 BlpI GC'TnA_GC Cuts at: 4663 4663 Size: 7246 Bme1580I G_kGCm'C Cuts at: 1790 2604 2712 2858 4478 4495 4516 5307 6463 Size: 814 108 146 1620 17 21 791 1156 Cuts at: 6463 1790 Size: 2573 Fragments arranged by size: 2573 1620 1156 814 791 146 108 21 17 BmgBI CAC'GTC Cuts at: 1318 4193 1318 Size: 2875 4371 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2160 2665 3337 Size: 416 53 83 399 24 743 505 672 Cuts at: 3337 3702 4977 5935 442 Size: 365 1275 958 1753 Fragments arranged by size: 1753 1275 958 743 672 505 416 399 365 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7246 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 2576 2941 3161 3680 3878 4360 5906 Size: 1142 195 365 220 519 198 482 1546 Cuts at: 5906 1239 Size: 2579 Fragments arranged by size: 2579 1546 1142 519 482 365 220 198 195 Bpu10I CC'TnA_GC Cuts at: 2606 2606 Size: 7246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2015 2525 2714 3652 4088 5371 6236 6534 2015 Size: 510 189 938 436 1283 865 298 2727 Fragments arranged by size: 2727 1283 938 865 510 436 298 189 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2112 3224 5909 882 Size: 617 613 1112 2685 2219 Fragments arranged by size: 2685 2219 1112 617 613 BsaAI yAC'GTr Cuts at: 493 1204 3415 5037 493 Size: 711 2211 1622 2702 Fragments arranged by size: 2702 2211 1622 711 BsaBI GATnn'nnATC Cuts at: 7240 7240 Size: 7246 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2916 3655 3914 4109 Size: 53 83 186 936 1383 739 259 195 Cuts at: 4109 5053 5435 6070 275 Size: 944 382 635 1451 Fragments arranged by size: 1451 1383 944 936 739 635 382 259 195 186 83 53 BsaWI w'CCGG_w Cuts at: 3537 5679 6077 6351 6498 6909 3537 Size: 2142 398 274 147 411 3874 Fragments arranged by size: 3874 2142 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2201 2231 3098 3128 4203 Size: 30 165 30 798 30 867 30 1075 Cuts at: 4203 4233 1178 Size: 30 4191 Fragments arranged by size: 4191 1075 867 798 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2103 2378 2573 3281 3284 3556 3757 3881 Size: 917 275 195 708 3 272 201 124 Cuts at: 3881 4211 4214 1186 Size: 330 3 4218 Fragments arranged by size: 4218 917 708 330 275 272 201 195 124 3 3 BseYI C'CCAG_C Cuts at: 3078 3693 3823 3967 4355 6449 6945 3078 Size: 615 130 144 388 2094 496 3379 Fragments arranged by size: 3379 2094 615 496 388 144 130 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2675 2684 3536 4116 4377 1163 Size: 573 939 9 852 580 261 4032 Fragments arranged by size: 4032 939 852 580 573 261 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3410 4068 4696 5457 6485 1540 Size: 275 186 1409 658 628 761 1028 2301 Fragments arranged by size: 2301 1409 1028 761 658 628 275 186 BsiHKAI G_wGCw'C Cuts at: 2617 2784 3333 4408 5307 5392 6463 2617 Size: 167 549 1075 899 85 1071 3400 Fragments arranged by size: 3400 1075 1071 899 549 167 85 BsmI GAATG_Cn' Cuts at: 4757 4850 4757 Size: 93 7153 BsmBI CGTCTCn'nnnn_ Cuts at: 2235 2235 Size: 7246 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2039 2166 2565 2604 2617 2712 Size: 399 223 26 127 399 39 13 95 Cuts at: 2712 2784 2858 3054 3333 3864 3873 4187 4206 Size: 72 74 196 279 531 9 314 19 Cuts at: 4206 4408 4478 4495 4516 4565 5307 5392 6463 Size: 202 70 17 21 49 742 85 1071 Cuts at: 6463 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 531 399 399 314 279 223 202 196 127 95 85 74 72 70 49 39 26 21 19 17 13 9 BspEI T'CCGG_A Cuts at: 6909 6909 Size: 7246 BspHI T'CATG_A Cuts at: 5135 6865 5135 Size: 1730 5516 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2697 3050 3342 3382 3993 4462 4601 844 Size: 1853 353 292 40 611 469 139 3489 Fragments arranged by size: 3489 1853 611 469 353 292 139 40 BsrBI CCG'CTC Cuts at: 1394 1823 3284 3682 4087 5133 1394 Size: 429 1461 398 405 1046 3507 Fragments arranged by size: 3507 1461 1046 429 405 398 BsrDI GCAATG_nn' Cuts at: 65 1248 2532 3679 5741 5915 65 Size: 1183 1284 1147 2062 174 1396 Fragments arranged by size: 2062 1396 1284 1183 1147 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2094 2594 5890 6077 1700 Size: 110 284 500 3296 187 2869 Fragments arranged by size: 3296 2869 500 284 187 110 BsrGI T'GTAC_A Cuts at: 96 2397 96 Size: 2301 4945 BssHII G'CGCG_C Cuts at: 1925 3287 3458 1925 Size: 1362 171 5713 Fragments arranged by size: 5713 1362 171 BssSI C'ACGA_G Cuts at: 5306 6318 5306 Size: 1012 6234 BstAPI GCAn_nnn'nTGC Cuts at: 794 2689 2982 794 Size: 1895 293 5058 Fragments arranged by size: 5058 1895 293 BstBI TT'CG_AA Cuts at: 6050 6050 Size: 7246 BstEII G'GTnAC_C Cuts at: 1184 2198 3836 1184 Size: 1014 1638 4594 Fragments arranged by size: 4594 1638 1014 BstXI CCAn_nnnn'nTGG Cuts at: 2270 3607 4507 2270 Size: 1337 900 5009 Fragments arranged by size: 5009 1337 900 BstYI r'GATC_y Cuts at: 1972 3210 3806 4576 4943 5328 5345 6797 6883 Size: 1238 596 770 367 385 17 1452 86 Cuts at: 6883 6895 7241 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1238 770 596 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2100 2189 2877 3802 2100 Size: 89 688 925 5544 Fragments arranged by size: 5544 925 688 89 BtgI C'CryG_G Cuts at: 513 1065 2004 2674 2850 2971 3167 3427 3845 Size: 552 939 670 176 121 196 260 418 Cuts at: 3845 4207 4361 4523 513 Size: 362 154 162 3236 Fragments arranged by size: 3236 939 670 552 418 362 260 196 176 162 154 121 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6507 Fragments arranged by size: 6507 683 56 BtsI GCAGTG_nn' Cuts at: 2065 2692 2917 3023 4762 5556 5576 2065 Size: 627 225 106 1739 794 20 3735 Fragments arranged by size: 3735 1739 794 627 225 106 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7246 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7211 DraI TTT'AAA Cuts at: 4550 4550 Size: 7246 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6253 809 Size: 507 4937 1802 Fragments arranged by size: 4937 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2272 2337 2619 Size: 54 1425 50 275 18 442 65 282 Cuts at: 2619 3069 3976 4693 5582 7211 8 Size: 450 907 717 889 1629 43 Fragments arranged by size: 1629 1425 907 889 717 450 442 282 275 65 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4693 1537 Size: 275 2881 4090 Fragments arranged by size: 4090 2881 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2012 2030 2510 3227 5176 1353 Size: 659 18 480 717 1949 3423 Fragments arranged by size: 3423 1949 717 659 480 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3323 5833 6207 6353 387 Size: 1064 138 1734 2510 374 146 1280 Fragments arranged by size: 2510 1734 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2615 2615 Size: 7246 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 2576 2813 2941 3161 3680 3878 Size: 393 749 195 237 128 220 519 198 Cuts at: 3878 4360 5309 5906 6693 6961 1239 Size: 482 949 597 787 268 1524 Fragments arranged by size: 1524 949 787 749 597 519 482 393 268 237 220 198 195 128 EcoNI CCTnn'n_nnAGG Cuts at: 2192 2192 Size: 7246 EcoO109I rG'GnC_Cy Cuts at: 2449 2601 2701 3088 3151 3704 3750 4474 4475 Size: 152 100 387 63 553 46 724 1 Cuts at: 4475 4519 4541 2449 Size: 44 22 5154 Fragments arranged by size: 5154 724 553 387 152 100 63 46 44 22 1 EcoRI G'AATT_C Cuts at: 4555 4933 4555 Size: 378 6868 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2041 2041 Size: 7246 FseI GG_CCGG'CC Cuts at: 2098 2098 Size: 7246 FspI TGC'GCA Cuts at: 5752 5752 Size: 7246 HaeII r_GCGC'y Cuts at: 1678 3140 3938 6393 1678 Size: 1462 798 2455 2531 Fragments arranged by size: 2531 2455 1462 798 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2201 2233 2717 2749 3098 Size: 32 135 32 629 32 484 32 349 Cuts at: 3098 3130 3202 3234 3284 3316 3779 3811 4203 Size: 32 72 32 50 32 463 32 392 Cuts at: 4203 4235 5887 5919 5961 5993 1373 Size: 32 1652 32 42 32 2626 Fragments arranged by size: 2626 1652 629 484 463 392 349 135 72 50 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2653 3013 4590 4836 669 Size: 988 281 715 360 1577 246 3079 Fragments arranged by size: 3079 1577 988 715 360 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7246 HpaI GTT'AAC Cuts at: 3013 4836 3013 Size: 1823 5423 KpnI G_GTAC'C Cuts at: 3778 4571 3778 Size: 793 6453 MfeI C'AATT_G Cuts at: 4845 4845 Size: 7246 MluI A'CGCG_T Cuts at: 7122 7122 Size: 7246 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2319 2760 3111 3588 6360 6544 1305 Size: 1014 441 351 477 2772 184 2007 Fragments arranged by size: 2772 2007 1014 477 441 351 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2274 2339 10 Size: 54 1425 343 442 65 4917 Fragments arranged by size: 4917 1425 442 343 65 54 MslI CAynn'nnrTG Cuts at: 518 1166 2468 2840 3512 5204 5563 5722 518 Size: 648 1302 372 672 1692 359 159 2042 Fragments arranged by size: 2042 1692 1302 672 648 372 359 159 MspA1I CmG'CkG Cuts at: 1606 2067 2120 2409 2852 3169 3298 3316 3429 Size: 461 53 289 443 317 129 18 113 Cuts at: 3429 3847 3931 4171 4209 4355 4662 5339 6487 Size: 418 84 240 38 146 307 677 1148 Cuts at: 6487 6732 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 461 443 418 317 307 289 245 240 146 129 113 84 53 38 18 NaeI GCC'GGC Cuts at: 2096 2096 Size: 7246 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5755 Fragments arranged by size: 5755 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7246 NgoMIV G'CCGG_C Cuts at: 2094 2094 Size: 7246 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7246 NotI GC'GGCC_GC Cuts at: 4693 4693 Size: 7246 NspI r_CATG'y Cuts at: 1132 1229 3487 3693 4604 6149 1132 Size: 97 2258 206 911 1545 2229 Fragments arranged by size: 2258 2229 1545 911 206 97 PasI CC'CwG_GG Cuts at: 1348 4285 1348 Size: 2937 4309 PciI A'CATG_T Cuts at: 6145 6145 Size: 7246 PflMI CCAn_nnn'nTGG Cuts at: 1108 2647 2972 1108 Size: 1539 325 5382 Fragments arranged by size: 5382 1539 325 PfoI T'CCnGG_A Cuts at: 2346 4422 2346 Size: 2076 5170 PmeI GTTT'AAAC Cuts at: 4550 4550 Size: 7246 PmlI CAC'GTG Cuts at: 3415 3415 Size: 7246 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5315 5347 6828 6860 5315 Size: 32 1481 32 5701 Fragments arranged by size: 5701 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2449 3088 3151 3704 3750 2449 Size: 639 63 553 46 5945 Fragments arranged by size: 5945 639 553 63 46 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7246 PsiI TTA'TAA Cuts at: 4816 4816 Size: 7246 PspOMI G'GGCC_C Cuts at: 2600 4474 2600 Size: 1874 5372 PstI C_TGCA'G Cuts at: 830 2757 3126 3390 3619 3990 4490 4598 830 Size: 1927 369 264 229 371 500 108 3478 Fragments arranged by size: 3478 1927 500 371 369 264 229 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7246 PvuII CAG'CTG Cuts at: 2409 3316 4355 2409 Size: 907 1039 5300 Fragments arranged by size: 5300 1039 907 SacI G_AGCT'C Cuts at: 2617 2617 Size: 7246 SacII CC_GC'GG Cuts at: 2853 3170 3430 3848 4210 2853 Size: 317 260 418 362 5889 Fragments arranged by size: 5889 418 362 317 260 SalI G'TCGA_C Cuts at: 1936 4588 1936 Size: 2652 4594 SanDI GG'GwC_CC Cuts at: 3704 3704 Size: 7246 SapI GCTCTTCn'nnn_ Cuts at: 2012 2510 2012 Size: 498 6748 SbfI CC_TGCA'GG Cuts at: 4598 4598 Size: 7246 ScaI AGT'ACT Cuts at: 5494 7206 5494 Size: 1712 5534 SfcI C'TryA_G Cuts at: 826 1045 2753 3122 3386 3615 3986 4486 4594 Size: 219 1708 369 264 229 371 500 108 Cuts at: 4594 5729 6410 6601 826 Size: 1135 681 191 1471 Fragments arranged by size: 1708 1471 1135 681 500 371 369 264 229 219 191 108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7246 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7246 SmaI CCC'GGG Cuts at: 1872 2222 4573 1872 Size: 350 2351 4545 Fragments arranged by size: 4545 2351 350 SmlI C'TyrA_G Cuts at: 820 1017 1943 2030 2540 2729 3667 4103 5350 Size: 197 926 87 510 189 938 436 1247 Cuts at: 5350 6251 6513 820 Size: 901 262 1553 Fragments arranged by size: 1553 1247 938 926 901 510 436 262 197 189 87 SnaBI TAC'GTA Cuts at: 493 5037 493 Size: 4544 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7246 SphI G_CATG'C Cuts at: 1132 3487 3693 4604 1132 Size: 2355 206 911 3774 Fragments arranged by size: 3774 2355 911 206 SspI AAT'ATT Cuts at: 5 52 5170 5 Size: 47 5118 2081 Fragments arranged by size: 5118 2081 47 StuI AGG'CCT Cuts at: 1517 2891 3521 3880 1517 Size: 1374 630 359 4883 Fragments arranged by size: 4883 1374 630 359 StyI C'CwwG_G Cuts at: 513 1065 2004 3240 3973 4685 513 Size: 552 939 1236 733 712 3074 Fragments arranged by size: 3074 1236 939 733 712 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2258 4082 4499 4516 5273 5290 5443 Size: 1255 678 1824 417 17 757 17 153 Cuts at: 5443 5628 6073 325 Size: 185 445 1498 Fragments arranged by size: 1824 1498 1255 757 678 445 417 185 153 17 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2397 5492 7204 Size: 274 80 33 51 1443 420 3095 1712 Cuts at: 7204 96 Size: 138 Fragments arranged by size: 3095 1712 1443 420 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2044 2076 3015 3047 7206 7238 2044 Size: 32 939 32 4159 32 2052 Fragments arranged by size: 4159 2052 939 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7246 XbaI T'CTAG_A Cuts at: 4582 4582 Size: 7246 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2572 3630 3775 2572 Size: 1058 145 6043 Fragments arranged by size: 6043 1058 145 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7246 XmaI C'CCGG_G Cuts at: 1870 2220 4571 1870 Size: 350 2351 4545 Fragments arranged by size: 4545 2351 350 XmnI GAAnn'nnTTC Cuts at: 5375 5375 Size: 7246 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2917 3656 5054 276 Size: 53 83 186 936 1383 739 1398 2468 Fragments arranged by size: 2468 1398 1383 936 739 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SexAI SfiI SfoI SrfI SwaI