################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsgI BsiWI BsmI Bsp1407I BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstXI BstYI BstZ17I Bsu36I CelII CpoI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FspI HgaI HindIII HpaI KasI KpnI Ksp632I KspI MamI MflI MluI MroI NarI NdeI NheI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 707 GACGTC GACGT^C AccI 508 GTAGAC GT^[AC][GT]AC AcyI 707 GACGTC G[GA]^CG[CT]C AflIII 446 ACACGT A^C[GA][CT]GT AspHI 73 GAGCAC G[AT]GC[AT]^C BanI 270 GGCACC G^G[CT][GA]CC BpmI 784 CTCCAG CTGGAG(16/14) BsaHI 707 GACGTC G[GA]^CG[CT]C BsaI 758 GGTCTC GGTCTC(1/5) BsaWI 662 ACCGGA [AT]^CCGG[AT] BseRI 502 GAGGAG GAGGAG(10/8) BsiHKAI 73 GAGCAC G[AT]GC[AT]^C BsmBI 190 GAGACG CGTCTC(1/5) BsmFI 813 GGGAC GGGAC(10/14) BspDI 409 ATCGAT AT^CGAT BspHI 899 TCATGA T^CATGA BstBI 49 TTCGAA TT^CGAA BstEII 30 GGTGACC G^GT[ACGT]ACC BstPI 30 GGTGACC G^GT[ACGT]ACC CfoI 515 GCGC GCG^C ClaI 409 ATCGAT AT^CGAT DraIII 586 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 30 GGTGACC G^GT[ACGT]ACC HaeII 514 AGCGCC [GA]GCGC^[CT] HhaI 515 GCGC GCG^C Hin1I 707 GACGTC G[GA]^CG[CT]C HinP1I 515 GCGC G^CGC HinfI 621 GACTC G^A[ACGT]TC MboII 311 TCTTC GAAGA(8/7) MfeI 153 CAATTG C^AATTG MseI 476 TTAA T^TAA MslI 912 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 153 CAATTG C^AATTG NcoI 678 CCATGG C^CATGG NotI 768 GCGGCCGC GC^GGCCGC NspV 49 TTCGAA TT^CGAA PleI 621 GACTC GAGTC(4/5) RcaI 899 TCATGA T^CATGA SfaNI 104 GCATC GCATC(5/9) SfcI 537 CTACAG C^T[GA][CT]AG SfuI 49 TTCGAA TT^CGAA SmaI 383 CCCGGG CCC^GGG StuI 672 AGGCCT AGG^CCT Tru9I 476 TTAA T^TAA Tsp509I 154 AATT ^AATT XmaCI 383 CCCGGG C^CCGGG XmaI 383 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 650 CGCG CG^CG 916 CGCG AfaI 488 GTAC GT^AC 702 GTAC AlwI 594 GATCC GGATC(4/5) 880 GATCC ApaI 380 GGGCCC GGGCC^C 921 GGGCCC AvaII 496 GGACC G^G[AT]CC 891 GGACC AvrII 631 CCTAGG C^CTAGG 855 CCTAGG BalI 179 TGGCCA TGG^CCA 365 TGGCCA BlnI 631 CCTAGG C^CTAGG 855 CCTAGG BsiEI 712 CGGCCG CG[GA][CT]^CG 769 CGGCCG BspMI 119 ACCTGC ACCTGC(4/8) 273 ACCTGC BsrDI 123 GCAATG GCAATG(2/0) 299 GCAATG BstF5I 912 CATCC GGATG(2/0) 654 GGATG BstUI 650 CGCG CG^CG 916 CGCG DraII 545 AGGCCCC [GA]G^G[ACGT]CC[CT] 921 GGGCCCC DsaI 338 CCGTGG C^C[GA][CT]GG 678 CCATGG EagI 712 CGGCCG C^GGCCG 769 CGGCCG EclXI 712 CGGCCG C^GGCCG 769 CGGCCG Eco52I 712 CGGCCG C^GGCCG 769 CGGCCG EcoO109I 545 AGGCCCC [GA]G^G[ACGT]CC[CT] 921 GGGCCCC FokI 912 CATCC GGATG(9/13) 654 GGATG FseI 571 GGCCGGCC GGCCGG^CC 713 GGCCGGCC HincII 61 GTCAAC GT[CT]^[GA]AC 373 GTCAAC HindII 61 GTCAAC GT[CT]^[GA]AC 373 GTCAAC HphI 30 GGTGA GGTGA(8/7) 420 GGTGA MaeIII 31 GTGAC ^GT[ACGT]AC 689 GTGAC MluNI 179 TGGCCA TGG^CCA 365 TGGCCA MscI 179 TGGCCA TGG^CCA 365 TGGCCA MvnI 650 CGCG CG^CG 916 CGCG PvuII 294 CAGCTG CAG^CTG 869 CAGCTG RsaI 488 GTAC GT^AC 702 GTAC TaqI 50 TCGA T^CGA 410 TCGA Tsp45I 31 GTGAC ^GT[GC]AC 689 GTGAC TthHB8I 50 TCGA T^CGA 410 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AvaI BanII BbvI BcnI BfaI BmyI BsaJI BsiYI BslI BsmAI BsoBI Bsp1286I BsrFI BsrI BssKI BstNI Cac8I Cfr10I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HpaII ItaI MaeI MaeII MboI MnlI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV Sau3AI Sau96I ScrFI StyI TseI TspRI