(Linear) (Six-base) MAPSORT of: KIEE4056.seq Check: 2351 from: 1 to: 930 KIEE4056.orf With 182 enzymes: SgfI * March 22, 2012 17:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 282 930 Size: 282 648 AatII G_ACGT'C Cuts at: 0 711 930 Size: 711 219 AccI GT'mk_AC Cuts at: 0 509 930 Size: 509 421 AflIII A'CryG_T Cuts at: 0 446 930 Size: 446 484 ApaI G_GGCC'C Cuts at: 0 384 925 930 Size: 384 541 5 Fragments arranged by size: 541 384 5 AvaI C'yCGr_G Cuts at: 0 383 433 577 930 Size: 383 50 144 353 Fragments arranged by size: 383 353 144 50 AvrII C'CTAG_G Cuts at: 0 631 855 930 Size: 631 224 75 Fragments arranged by size: 631 224 75 BanI G'GyrC_C Cuts at: 0 270 930 Size: 270 660 BanII G_rGCy'C Cuts at: 0 384 427 434 440 617 925 930 Size: 384 43 7 6 177 308 5 Fragments arranged by size: 384 308 177 43 7 6 5 Bme1580I G_kGCm'C Cuts at: 0 19 292 384 925 930 Size: 19 273 92 541 5 Fragments arranged by size: 541 273 92 19 5 BmgBI CAC'GTC Cuts at: 0 449 930 Size: 449 481 BmrI ACTGGGnnnn_n' Cuts at: 0 234 387 403 930 Size: 234 153 16 527 Fragments arranged by size: 527 234 153 16 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 769 930 Size: 769 161 Bpu10I CC'TnA_GC Cuts at: 0 611 742 930 Size: 611 131 188 Fragments arranged by size: 611 188 131 BsaI GGTCTCn'nnnn_ Cuts at: 0 764 930 Size: 764 166 BsaHI Gr'CG_yC Cuts at: 0 708 930 Size: 708 222 BsaWI w'CCGG_w Cuts at: 0 662 930 Size: 662 268 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 383 413 659 689 930 Size: 383 30 246 30 241 Fragments arranged by size: 383 246 241 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 517 930 Size: 517 413 BseYI C'CCAG_C Cuts at: 0 251 292 528 558 754 819 930 Size: 251 41 236 30 196 65 111 Fragments arranged by size: 251 236 196 111 65 41 30 BsiEI CG_ry'CG Cuts at: 0 715 772 930 Size: 715 57 158 Fragments arranged by size: 715 158 57 BsiHKAI G_wGCw'C Cuts at: 0 77 930 Size: 77 853 BsmBI CGTCTCn'nnnn_ Cuts at: 0 184 930 Size: 184 746 Bsp1286I G_dGCh'C Cuts at: 0 19 77 292 384 427 434 440 617 Size: 19 58 215 92 43 7 6 177 Cuts at: 617 925 930 Size: 308 5 Fragments arranged by size: 308 215 177 92 58 43 19 7 6 5 BspHI T'CATG_A Cuts at: 0 899 930 Size: 899 31 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 128 282 930 Size: 128 154 648 Fragments arranged by size: 648 154 128 BsrDI GCAATG_nn' Cuts at: 0 130 306 930 Size: 130 176 624 Fragments arranged by size: 624 176 130 BsrFI r'CCGG_y Cuts at: 0 277 572 714 930 Size: 277 295 142 216 Fragments arranged by size: 295 277 216 142 BstBI TT'CG_AA Cuts at: 0 50 930 Size: 50 880 BstEII G'GTnAC_C Cuts at: 0 30 930 Size: 30 900 BtgI C'CryG_G Cuts at: 0 338 678 930 Size: 338 340 252 Fragments arranged by size: 340 338 252 BtsI GCAGTG_nn' Cuts at: 0 75 930 Size: 75 855 ClaI AT'CG_AT Cuts at: 0 410 930 Size: 410 520 DraIII CAC_nnn'GTG Cuts at: 0 591 930 Size: 591 339 EaeI y'GGCC_r Cuts at: 0 179 365 712 769 930 Size: 179 186 347 57 161 Fragments arranged by size: 347 186 179 161 57 EagI C'GGCC_G Cuts at: 0 712 769 930 Size: 712 57 161 Fragments arranged by size: 712 161 57 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 769 930 Size: 769 161 EcoO109I rG'GnC_Cy Cuts at: 0 546 922 930 Size: 546 376 8 Fragments arranged by size: 546 376 8 FseI GG_CCGG'CC Cuts at: 0 576 718 930 Size: 576 142 212 Fragments arranged by size: 576 212 142 HaeII r_GCGC'y Cuts at: 0 518 930 Size: 518 412 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 383 415 502 534 930 Size: 383 32 87 32 396 Fragments arranged by size: 396 383 87 32 32 HincII GTy'rAC Cuts at: 0 63 375 930 Size: 63 312 555 Fragments arranged by size: 555 312 63 MfeI C'AATT_G Cuts at: 0 153 930 Size: 153 777 MscI TGG'CCA Cuts at: 0 181 367 930 Size: 181 186 563 Fragments arranged by size: 563 186 181 MslI CAynn'nnrTG Cuts at: 0 916 930 Size: 916 14 MspA1I CmG'CkG Cuts at: 0 296 774 789 871 930 Size: 296 478 15 82 59 Fragments arranged by size: 478 296 82 59 15 NaeI GCC'GGC Cuts at: 0 279 574 716 930 Size: 279 295 142 214 Fragments arranged by size: 295 279 214 142 NcoI C'CATG_G Cuts at: 0 678 930 Size: 678 252 NgoMIV G'CCGG_C Cuts at: 0 277 572 714 930 Size: 277 295 142 216 Fragments arranged by size: 295 277 216 142 NotI GC'GGCC_GC Cuts at: 0 769 930 Size: 769 161 PspOMI G'GGCC_C Cuts at: 0 380 921 930 Size: 380 541 9 Fragments arranged by size: 541 380 9 PvuII CAG'CTG Cuts at: 0 296 871 930 Size: 296 575 59 Fragments arranged by size: 575 296 59 SfcI C'TryA_G Cuts at: 0 537 930 Size: 537 393 SmaI CCC'GGG Cuts at: 0 385 930 Size: 385 545 SrfI GCCC'GGGC Cuts at: 0 385 930 Size: 385 545 StuI AGG'CCT Cuts at: 0 674 930 Size: 674 256 StyI C'CwwG_G Cuts at: 0 631 678 719 855 930 Size: 631 47 41 136 75 Fragments arranged by size: 631 136 75 47 41 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 659 691 930 Size: 659 32 239 Fragments arranged by size: 659 239 32 XmaI C'CCGG_G Cuts at: 0 383 930 Size: 383 547 ZraI GAC'GTC Cuts at: 0 709 930 Size: 709 221 Enzymes that do cut: AarI AatII AccI AflIII ApaI AvaI AvrII BanI BanII Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstBI BstEII BtgI BtsI ClaI DraIII EaeI EagI Eco57MI EcoO109I FseI HaeII Hin4I HincII MfeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI PspOMI PvuII SfcI SmaI SrfI StuI StyI TstI XmaI ZraI Enzymes that do not cut: Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI BplI BpuEI BsaAI BsaBI BsgI BsiWI BsmI BspEI BsrBI BsrGI BssHII BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgZI CspCI DraI DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FspI FspAI HindIII HpaI KasI KpnI MluI MmeI NarI NdeI NheI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SspI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmnI