(Circular) (Six-base) MAPSORT of: pFN21AE3532.seq Check: 8068 from: 1 to: 5956 pFN21AE3532 5956 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2071 2071 Size: 5956 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3299 1937 Size: 1362 4594 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4019 5403 5671 1632 Size: 2387 1384 268 1917 Fragments arranged by size: 2387 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 3084 1166 Size: 1918 4038 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2964 1184 Size: 1780 4176 AlwNI CAG_nnn'CTG Cuts at: 2914 3699 5271 2914 Size: 785 1572 3599 Fragments arranged by size: 3599 1572 785 ApaI G_GGCC'C Cuts at: 2280 2515 2280 Size: 235 5721 ApaLI G'TGCA_C Cuts at: 2894 4013 5169 2894 Size: 1119 1156 3681 Fragments arranged by size: 3681 1156 1119 ApoI r'AATT_y Cuts at: 1493 3265 3491 3643 5752 1493 Size: 1772 226 152 2109 1697 Fragments arranged by size: 2109 1772 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 3281 1870 Size: 73 1338 4545 Fragments arranged by size: 4545 1338 73 AvrII C'CTAG_G Cuts at: 3137 3137 Size: 5956 BamHI G'GATC_C Cuts at: 3178 3286 3653 3178 Size: 108 367 5481 Fragments arranged by size: 5481 367 108 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2047 2493 2605 2672 Size: 325 217 627 242 18 446 112 67 Cuts at: 2672 3036 3277 4732 618 Size: 364 241 1455 1842 Fragments arranged by size: 1842 1455 627 446 364 325 242 241 217 112 67 18 BanII G_rGCy'C Cuts at: 1391 2280 2515 3275 1391 Size: 889 235 760 4072 Fragments arranged by size: 4072 889 760 235 BbeI G_GCGC'C Cuts at: 2497 2609 2676 2497 Size: 112 67 5777 Fragments arranged by size: 5777 112 67 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4985 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4147 4181 1903 Size: 34 2210 34 3678 Fragments arranged by size: 3678 2210 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2290 2603 2699 Size: 107 122 71 631 605 618 313 96 Cuts at: 2699 4567 136 Size: 1868 1525 Fragments arranged by size: 1868 1525 631 618 605 313 122 107 96 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 2163 2280 2503 2515 2898 2985 4017 5173 Size: 373 117 223 12 383 87 1032 1156 Cuts at: 5173 1790 Size: 2573 Fragments arranged by size: 2573 1156 1032 383 373 223 117 87 12 BmgBI CAC'GTC Cuts at: 1318 3086 1318 Size: 1768 4188 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3117 3687 4645 Size: 416 53 83 399 24 1700 570 958 Cuts at: 4645 442 Size: 1753 Fragments arranged by size: 1753 1700 958 570 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2429 3019 2429 Size: 590 5366 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2648 4616 1239 Size: 1409 1968 2579 Fragments arranged by size: 2579 1968 1409 Bpu10I CC'TnA_GC Cuts at: 2915 2915 Size: 5956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 4946 5244 4081 Size: 865 298 4793 Fragments arranged by size: 4793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4619 882 Size: 617 3120 2219 Fragments arranged by size: 3120 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2494 2606 2673 3763 Size: 53 83 186 936 961 112 67 1090 Cuts at: 3763 4145 4780 275 Size: 382 635 1451 Fragments arranged by size: 1451 1090 961 936 635 382 186 112 83 67 53 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5731 Fragments arranged by size: 5731 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3194 1186 Size: 2008 3948 BseYI C'CCAG_C Cuts at: 2133 2978 5159 5655 2133 Size: 845 2181 496 2434 Fragments arranged by size: 2434 2181 845 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5383 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2393 2661 3406 4167 5195 1540 Size: 275 186 392 268 745 761 1028 2301 Fragments arranged by size: 2301 1028 761 745 392 275 268 186 BsiHKAI G_wGCw'C Cuts at: 2484 2898 2921 3015 3032 4017 4102 5173 2484 Size: 414 23 94 17 985 85 1071 3267 Fragments arranged by size: 3267 1071 985 414 94 85 23 17 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 BsmBI CGTCTCn'nnnn_ Cuts at: 2174 2174 Size: 5956 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2163 2280 2484 2503 2515 2898 2921 Size: 399 373 117 204 19 12 383 23 Cuts at: 2921 2985 3015 3032 3275 4017 4102 5173 1391 Size: 64 30 17 243 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 383 373 243 204 117 85 64 30 23 19 17 12 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2071 2932 3311 844 Size: 1227 861 379 3489 Fragments arranged by size: 3489 1227 861 379 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4451 4625 65 Size: 1183 3203 174 1396 Fragments arranged by size: 3203 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2742 2898 3211 4600 4787 1700 Size: 110 932 156 313 1389 187 2869 Fragments arranged by size: 2869 1389 932 313 187 156 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 2562 1925 Size: 637 5319 BssSI C'ACGA_G Cuts at: 4016 5028 4016 Size: 1012 4944 BstAPI GCAn_nnn'nTGC Cuts at: 794 3036 794 Size: 2242 3714 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstXI CCAn_nnnn'nTGG Cuts at: 2272 2875 3182 2272 Size: 603 307 5046 Fragments arranged by size: 5046 603 307 BstYI r'GATC_y Cuts at: 1972 3178 3286 3653 4038 4055 5507 5593 5605 Size: 1206 108 367 385 17 1452 86 12 Cuts at: 5605 5951 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1206 385 367 346 108 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2676 513 Size: 552 939 672 3793 Fragments arranged by size: 3793 939 672 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2678 487 Size: 683 56 1452 3765 Fragments arranged by size: 3765 1452 683 56 BtsI GCAGTG_nn' Cuts at: 3472 4266 4286 3472 Size: 794 20 5142 Fragments arranged by size: 5142 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DraIII CAC_nnn'GTG Cuts at: 3036 3036 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2390 2658 3051 Size: 54 1425 50 275 18 560 268 393 Cuts at: 3051 3209 3403 4292 5921 8 Size: 158 194 889 1629 43 Fragments arranged by size: 1629 1425 889 560 393 275 268 194 158 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2390 2658 3403 1537 Size: 275 578 268 745 4090 Fragments arranged by size: 4090 745 578 275 268 EarI CTCTTCn'nnn_ Cuts at: 1353 2120 2237 2849 3886 1353 Size: 767 117 612 1037 3423 Fragments arranged by size: 3423 1037 767 612 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2804 4543 4917 5063 387 Size: 1064 138 1215 1739 374 146 1280 Fragments arranged by size: 1739 1280 1215 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2648 4019 4616 5403 5671 1239 Size: 393 1016 1371 597 787 268 1524 Fragments arranged by size: 1524 1371 1016 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2276 2277 2427 2439 2512 2911 3119 3141 2276 Size: 1 150 12 73 399 208 22 5091 Fragments arranged by size: 5091 399 208 150 73 22 12 1 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 2497 2609 2676 5103 1678 Size: 819 112 67 2427 2531 Fragments arranged by size: 2531 2427 819 112 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4597 4629 4671 4703 1373 Size: 32 135 32 3025 32 42 32 2626 Fragments arranged by size: 3025 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KasI G'GCGC_C Cuts at: 2493 2605 2672 2493 Size: 112 67 5777 Fragments arranged by size: 5777 112 67 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5254 1305 Size: 3765 184 2007 Fragments arranged by size: 3765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4134 Fragments arranged by size: 4134 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3084 3914 4273 4432 518 Size: 648 1918 830 359 159 2042 Fragments arranged by size: 2042 1918 830 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2087 3372 4049 5197 5442 1606 Size: 481 1285 677 1148 245 2120 Fragments arranged by size: 2120 1285 1148 677 481 245 NaeI GCC'GGC Cuts at: 2744 3213 2744 Size: 469 5487 NarI GG'CG_CC Cuts at: 2494 2606 2673 2494 Size: 112 67 5777 Fragments arranged by size: 5777 112 67 NcoI C'CATG_G Cuts at: 513 1065 2004 2676 513 Size: 552 939 672 3793 Fragments arranged by size: 3793 939 672 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NgoMIV G'CCGG_C Cuts at: 2742 3211 2742 Size: 469 5487 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 2658 3403 2658 Size: 745 5211 NspI r_CATG'y Cuts at: 1132 1229 3314 4859 1132 Size: 97 2085 1545 2229 Fragments arranged by size: 2229 2085 1545 97 PasI CC'CwG_GG Cuts at: 1348 2074 2508 1348 Size: 726 434 4796 Fragments arranged by size: 4796 726 434 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5956 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2911 2911 Size: 5956 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PspOMI G'GGCC_C Cuts at: 2276 2511 2276 Size: 235 5721 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2804 2836 2804 Size: 32 5924 PstI C_TGCA'G Cuts at: 830 2322 3308 830 Size: 1492 986 3478 Fragments arranged by size: 3478 1492 986 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 PvuII CAG'CTG Cuts at: 2087 2087 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SapI GCTCTTCn'nnn_ Cuts at: 2120 2120 Size: 5956 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SexAI A'CCwGG_T Cuts at: 2793 2823 3032 2793 Size: 30 209 5717 Fragments arranged by size: 5717 209 30 SfcI C'TryA_G Cuts at: 826 1045 2318 3304 4439 5120 5311 826 Size: 219 1273 986 1135 681 191 1471 Fragments arranged by size: 1471 1273 1135 986 681 219 191 SfoI GGC'GCC Cuts at: 2495 2607 2674 2495 Size: 112 67 5777 Fragments arranged by size: 5777 112 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4060 4961 5223 820 Size: 197 926 2117 901 262 1553 Fragments arranged by size: 2117 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5956 StyI C'CwwG_G Cuts at: 513 1065 2004 2376 2634 2676 2727 3137 3395 Size: 552 939 372 258 42 51 410 258 Cuts at: 3395 513 Size: 3074 Fragments arranged by size: 3074 939 552 410 372 258 258 51 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2657 2946 3983 4000 4153 4338 4783 Size: 1255 1077 289 1037 17 153 185 445 Cuts at: 4783 325 Size: 1498 Fragments arranged by size: 1498 1255 1077 1037 445 289 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4202 5914 96 Size: 274 80 33 51 1443 2225 1712 138 Fragments arranged by size: 2225 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5916 5948 5916 Size: 32 5924 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2872 2875 2933 2872 Size: 3 58 5895 Fragments arranged by size: 5895 58 3 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SacII SanDI SfiI SrfI SwaI