################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HincII HindII HinfI HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboII MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### AcyI 12 GACGCC G[GA]^CG[CT]C AluI 101 AGCT AG^CT BamHI 170 GGATCC G^GATCC BcnI 47 CCCGG CC^[GC]GG BpmI 184 CTGGAG CTGGAG(16/14) BsaHI 12 GACGCC G[GA]^CG[CT]C BsaI 187 GAGACC GGTCTC(1/5) BsmAI 187 GAGAC GTCTC(1/5) BsrI 58 CCAGT ACTGG(1/-1) BssHII 66 GCGCGC G^CGCGC BstNI 183 CCTGG CC^[AT]GG BstXI 160 CCAGCGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 45 GGCCC G^G[ACGT]CC EaeI 119 TGGCCG [CT]^GGCC[GA] EcoNI 191 CCTACAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 183 CCTGG ^CC[AT]GG HgaI 12 GACGC GACGC(5/10) Hin1I 12 GACGCC G[GA]^CG[CT]C HindIII 100 AAGCTT A^AGCTT MvaI 183 CCTGG CC^[AT]GG NciI 47 CCCGG CC^[GC]GG NlaIII 111 CATG CATG^ Sau96I 45 GGCCC G^G[ACGT]CC SfcI 192 CTACAG C^T[GA][CT]AG Tsp509I 116 AATT ^AATT TspRI 59 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 65 CGCG CG^CG 67 CGCG BssKI 47 CCCGG ^CC[ACGT]GG 183 CCTGG BstUI 65 CGCG CG^CG 67 CGCG BstYI 74 AGATCC [GA]^GATC[CT] 170 GGATCC CfoI 66 GCGC GCG^C 68 GCGC FauI 27 GCGGG CCCGC(4/6) 163 GCGGG HapII 48 CCGG C^CGG 91 CCGG HhaI 66 GCGC GCG^C 68 GCGC HinP1I 66 GCGC G^CGC 68 GCGC HpaII 48 CCGG C^CGG 91 CCGG MaeIII 145 GTGAC ^GT[ACGT]AC 177 GTCAC MflI 74 AGATCC [GA]^GATC[CT] 170 GGATCC MspA1I 16 CCGCTG C[AC]G^C[GT]G 161 CAGCGG MspI 48 CCGG C^CGG 91 CCGG MvnI 65 CGCG CG^CG 67 CGCG NlaIV 53 GGCTCC GG[ACGT]^[ACGT]CC 170 GGATCC ScrFI 47 CCCGG CC^[ACGT]GG 183 CCTGG Tsp45I 145 GTGAC ^GT[GC]AC 177 GTCAC XhoII 74 AGATCC [GA]^GATC[CT] 170 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AciI AlwI BbvI BsiYI BslI Cac8I DpnII Fnu4HI HaeIII ItaI MboI MnlI MwoI NdeII Sau3AI TseI