(Circular) (Six-base) MAPSORT of: pFN21AE2200.seq Check: 8657 from: 1 to: 6256 pFN21AE2200 6256 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3599 1937 Size: 1662 4594 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3073 3422 3433 4319 5703 5971 1632 Size: 1441 349 11 886 1384 268 1917 Fragments arranged by size: 1917 1441 1384 886 349 268 11 AfeI AGC'GCT Cuts at: 2152 2152 Size: 6256 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 2024 3112 5155 6132 1203 Size: 821 1088 2043 977 1327 Fragments arranged by size: 2043 1327 1088 977 821 AgeI A'CCGG_T Cuts at: 5087 5087 Size: 6256 AhdI GACnn_n'nnGTC Cuts at: 4985 4985 Size: 6256 AleI CACnn'nnGTG Cuts at: 1166 3252 1166 Size: 2086 4170 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6256 AlwNI CAG_nnn'CTG Cuts at: 2473 3999 5571 2473 Size: 1526 1572 3158 Fragments arranged by size: 3158 1572 1526 ApaI G_GGCC'C Cuts at: 2541 2541 Size: 6256 ApaLI G'TGCA_C Cuts at: 4313 5469 4313 Size: 1156 5100 ApoI r'AATT_y Cuts at: 1493 3565 3791 3943 6052 1493 Size: 2072 226 152 2109 1697 Fragments arranged by size: 2109 2072 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 2110 2335 2621 3581 1870 Size: 73 167 225 286 960 4545 Fragments arranged by size: 4545 960 286 225 167 73 BamHI G'GATC_C Cuts at: 2746 3586 3953 2746 Size: 840 367 5049 Fragments arranged by size: 5049 840 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2073 2135 2311 2791 3577 Size: 325 217 627 286 62 176 480 786 Cuts at: 3577 5032 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 786 627 480 325 286 217 176 62 BanII G_rGCy'C Cuts at: 1391 2216 2541 2768 2972 3061 3575 1391 Size: 825 325 227 204 89 514 4072 Fragments arranged by size: 4072 825 514 325 227 204 89 BbeI G_GCGC'C Cuts at: 2139 2315 2139 Size: 176 6080 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3079 3410 928 Size: 971 1180 331 3774 Fragments arranged by size: 3774 1180 971 331 BbvCI CC'TCA_GC Cuts at: 2858 2858 Size: 6256 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4447 4481 1903 Size: 34 50 34 2426 34 3678 Fragments arranged by size: 3678 2426 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4148 5358 4148 Size: 1210 5046 BclI T'GATC_A Cuts at: 1548 1548 Size: 6256 BfrBI ATG'CAT Cuts at: 2913 3520 2913 Size: 607 5649 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2144 2763 4867 Size: 107 122 71 631 605 472 619 2104 Cuts at: 4867 136 Size: 1525 Fragments arranged by size: 2104 1525 631 619 605 472 122 107 71 BglII A'GATC_T Cuts at: 6251 6251 Size: 6256 BlpI GC'TnA_GC Cuts at: 3673 3673 Size: 6256 Bme1580I G_kGCm'C Cuts at: 1790 2284 2541 4317 5473 1790 Size: 494 257 1776 1156 2573 Fragments arranged by size: 2573 1776 1156 494 257 BmgBI CAC'GTC Cuts at: 1318 2221 3113 1318 Size: 903 892 4461 Fragments arranged by size: 4461 903 892 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3987 4945 442 Size: 416 53 83 399 24 2570 958 1753 Fragments arranged by size: 2570 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2956 3141 3527 4916 1239 Size: 908 809 185 386 1389 2579 Fragments arranged by size: 2579 1389 908 809 386 185 Bpu10I CC'TnA_GC Cuts at: 2474 2858 2474 Size: 384 5872 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4381 5246 5544 4381 Size: 865 298 5093 Fragments arranged by size: 5093 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3149 4919 882 Size: 617 1650 1770 2219 Fragments arranged by size: 2219 1770 1650 617 BsaAI yAC'GTr Cuts at: 493 1204 2025 4047 493 Size: 711 821 2022 2702 Fragments arranged by size: 2702 2022 821 711 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 2136 2186 2312 Size: 53 83 186 936 509 94 50 126 Cuts at: 2312 2567 2678 2864 4063 4445 5080 275 Size: 255 111 186 1199 382 635 1451 Fragments arranged by size: 1451 1199 936 635 509 382 255 186 186 126 111 94 83 53 50 BsaWI w'CCGG_w Cuts at: 4689 5087 5361 5508 5919 4689 Size: 398 274 147 411 5026 Fragments arranged by size: 5026 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 1178 Size: 30 165 30 624 30 5377 Fragments arranged by size: 5377 624 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2188 2477 2541 1186 Size: 1002 289 64 4901 Fragments arranged by size: 4901 1002 289 64 BseYI C'CCAG_C Cuts at: 2439 2558 2612 2681 2770 2901 2963 5459 5955 Size: 119 54 69 89 131 62 2496 496 Cuts at: 5955 2439 Size: 2740 Fragments arranged by size: 2740 2496 496 131 119 89 69 62 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2818 1163 Size: 573 1082 4601 Fragments arranged by size: 4601 1082 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3146 3706 4467 5495 1540 Size: 275 186 1145 560 761 1028 2301 Fragments arranged by size: 2301 1145 1028 761 560 275 186 BsiHKAI G_wGCw'C Cuts at: 2972 4317 4402 5473 2972 Size: 1345 85 1071 3755 Fragments arranged by size: 3755 1345 1071 85 BsmI GAATG_Cn' Cuts at: 2915 3096 3767 3860 2915 Size: 181 671 93 5311 Fragments arranged by size: 5311 671 181 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2049 2165 2049 Size: 116 6140 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2216 2284 2541 2768 2972 3061 3575 Size: 399 426 68 257 227 204 89 514 Cuts at: 3575 4317 4402 5473 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 514 426 399 257 227 204 89 85 68 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2616 3611 844 Size: 1772 995 3489 Fragments arranged by size: 3489 1772 995 BsrBI CCG'CTC Cuts at: 1394 1823 2061 2295 4143 1394 Size: 429 238 234 1848 3507 Fragments arranged by size: 3507 1848 429 238 234 BsrDI GCAATG_nn' Cuts at: 65 1248 4751 4925 65 Size: 1183 3503 174 1396 Fragments arranged by size: 3503 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2038 2314 2380 2837 4900 5087 1700 Size: 110 228 276 66 457 2063 187 2869 Fragments arranged by size: 2869 2063 457 276 228 187 110 66 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6256 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6256 BssSI C'ACGA_G Cuts at: 2459 4316 5328 2459 Size: 1857 1012 3387 Fragments arranged by size: 3387 1857 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 2662 1184 Size: 1478 4778 BstXI CCAn_nnnn'nTGG Cuts at: 2510 2510 Size: 6256 BstYI r'GATC_y Cuts at: 1972 2746 3586 3953 4338 4355 5807 5893 5905 Size: 774 840 367 385 17 1452 86 12 Cuts at: 5905 6251 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 840 774 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2179 2751 513 Size: 552 1114 572 4018 Fragments arranged by size: 4018 1114 572 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5517 Fragments arranged by size: 5517 683 56 BtsI GCAGTG_nn' Cuts at: 2312 2474 2881 3772 4566 4586 2312 Size: 162 407 891 794 20 3982 Fragments arranged by size: 3982 891 794 407 162 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 3560 3560 Size: 6256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2500 2575 3461 Size: 54 1425 50 275 18 670 75 886 Cuts at: 3461 3703 4592 6221 8 Size: 242 889 1629 43 Fragments arranged by size: 1629 1425 889 886 670 275 242 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3703 1537 Size: 275 1891 4090 Fragments arranged by size: 4090 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4186 1353 Size: 2833 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2376 2544 3494 4843 5217 5363 Size: 1064 138 787 168 950 1349 374 146 Cuts at: 5363 387 Size: 1280 Fragments arranged by size: 1349 1280 1064 950 787 374 168 146 138 EcoICRI GAG'CTC Cuts at: 2970 2970 Size: 6256 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2956 3073 3141 3422 3433 3527 Size: 393 515 809 117 68 281 11 94 Cuts at: 3527 4319 4916 5703 5971 1239 Size: 792 597 787 268 1524 Fragments arranged by size: 1524 809 792 787 597 515 393 281 268 117 94 68 11 EcoO109I rG'GnC_Cy Cuts at: 2011 2107 2227 2538 2011 Size: 96 120 311 5729 Fragments arranged by size: 5729 311 120 96 EcoRI G'AATT_C Cuts at: 3565 3943 3565 Size: 378 5878 FspI TGC'GCA Cuts at: 4762 4762 Size: 6256 HaeII r_GCGC'y Cuts at: 1678 2139 2154 2315 2343 2481 3554 5403 1678 Size: 461 15 161 28 138 1073 1849 2531 Fragments arranged by size: 2531 1849 1073 461 161 138 28 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2908 2940 4897 Size: 32 135 32 455 32 849 32 1957 Cuts at: 4897 4929 4971 5003 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1957 849 455 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3600 3846 669 Size: 988 281 1662 246 3079 Fragments arranged by size: 3079 1662 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6256 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KasI G'GCGC_C Cuts at: 2135 2311 2135 Size: 176 6080 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2241 3417 5370 5554 1305 Size: 936 1176 1953 184 2007 Fragments arranged by size: 2007 1953 1176 936 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3463 10 Size: 54 1425 343 1631 2803 Fragments arranged by size: 2803 1631 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3252 3268 3342 3358 4214 4573 4732 Size: 648 2086 16 74 16 856 359 159 Cuts at: 4732 518 Size: 2042 Fragments arranged by size: 2086 2042 856 648 359 159 74 16 16 MspA1I CmG'CkG Cuts at: 1606 2181 2616 2832 3453 3547 3672 4349 5497 Size: 575 435 216 621 94 125 677 1148 Cuts at: 5497 5742 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 621 575 435 245 216 125 94 NaeI GCC'GGC Cuts at: 2040 2040 Size: 6256 NarI GG'CG_CC Cuts at: 2136 2312 2136 Size: 176 6080 NcoI C'CATG_G Cuts at: 513 1065 2751 513 Size: 552 1686 4018 Fragments arranged by size: 4018 1686 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NgoMIV G'CCGG_C Cuts at: 2038 2038 Size: 6256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NsiI A_TGCA'T Cuts at: 2915 3522 2915 Size: 607 5649 NspI r_CATG'y Cuts at: 1132 1229 3520 3614 5159 1132 Size: 97 2291 94 1545 2229 Fragments arranged by size: 2291 2229 1545 97 94 PasI CC'CwG_GG Cuts at: 1348 2260 1348 Size: 912 5344 PciI A'CATG_T Cuts at: 5155 5155 Size: 6256 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6256 PfoI T'CCnGG_A Cuts at: 2031 2103 2031 Size: 72 6184 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PmlI CAC'GTG Cuts at: 2025 2025 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2011 2107 2227 2011 Size: 96 120 6040 Fragments arranged by size: 6040 120 96 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PspOMI G'GGCC_C Cuts at: 2537 2537 Size: 6256 PstI C_TGCA'G Cuts at: 830 3608 830 Size: 2778 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 PvuII CAG'CTG Cuts at: 2616 2832 3453 3547 2616 Size: 216 621 94 5325 Fragments arranged by size: 5325 621 216 94 RsrII CG'GwC_CG Cuts at: 3390 3390 Size: 6256 SacI G_AGCT'C Cuts at: 2972 2972 Size: 6256 SacII CC_GC'GG Cuts at: 2182 2182 Size: 6256 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SanDI GG'GwC_CC Cuts at: 2107 2107 Size: 6256 SbfI CC_TGCA'GG Cuts at: 3608 3608 Size: 6256 ScaI AGT'ACT Cuts at: 2786 4504 6216 2786 Size: 1718 1712 2826 Fragments arranged by size: 2826 1718 1712 SfcI C'TryA_G Cuts at: 826 1045 2078 2869 3604 4739 5420 5611 826 Size: 219 1033 791 735 1135 681 191 1471 Fragments arranged by size: 1471 1135 1033 791 735 681 219 191 SfoI GGC'GCC Cuts at: 2137 2313 2137 Size: 176 6080 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 2314 1700 Size: 614 5642 SmaI CCC'GGG Cuts at: 1872 2112 2337 3583 1872 Size: 240 225 1246 4545 Fragments arranged by size: 4545 1246 240 225 SmlI C'TyrA_G Cuts at: 820 1017 1943 2621 4360 5261 5523 820 Size: 197 926 678 1739 901 262 1553 Fragments arranged by size: 1739 1553 926 901 678 262 197 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 3614 1132 Size: 2482 3774 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6256 StyI C'CwwG_G Cuts at: 513 1065 2751 3695 513 Size: 552 1686 944 3074 Fragments arranged by size: 3074 1686 944 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2061 2252 2534 2762 4283 4300 4453 Size: 1255 481 191 282 228 1521 17 153 Cuts at: 4453 4638 5083 325 Size: 185 445 1498 Fragments arranged by size: 1521 1498 1255 481 445 282 228 191 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2784 4502 6214 Size: 274 80 33 51 1443 807 1718 1712 Cuts at: 6214 96 Size: 138 Fragments arranged by size: 1718 1712 1443 807 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6216 6248 6216 Size: 32 6224 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6256 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3544 3544 Size: 6256 XhoI C'TCGA_G Cuts at: 1943 2621 1943 Size: 678 5578 XmaI C'CCGG_G Cuts at: 1870 2110 2335 3581 1870 Size: 240 225 1246 4545 Fragments arranged by size: 4545 1246 240 225 XmnI GAAnn'nnTTC Cuts at: 3298 3353 4385 3298 Size: 55 1032 5169 Fragments arranged by size: 5169 1032 55 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI PacI PspXI PsrI SapI SexAI SfiI SrfI SwaI