################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI AspEI AspI AvaI AvrII BalI BbeI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DsaI Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI Hin1I HincII HindII HpaI HphI KasI KspI MamI MfeI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### Acc65I 386 GGTACC G^GTACC AflIII 890 ACGCGT A^C[GA][CT]GT AlwNI 853 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 925 CCTGAGG CC^T[ACGT]AGG Aor51HI 356 AGCGCT AGC^GCT ApaI 440 GGGCCC GGGCC^C Asp700I 174 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 386 GGTACC G^GTACC AviII 167 TGCGCA TGC^GCA BanI 386 GGTACC G^G[CT][GA]CC BbrPI 727 CACGTG CAC^GTG BbsI 670 GTCTTC GAAGAC(2/6) BbvCI 225 CCTCAGC CCTCAGC(-5/-2) BciVI 266 GTATCC GTATCC(6/5) BclI 596 TGATCA T^GATCA BcnI 617 CCCGG CC^[GC]GG BfaI 763 CTAG C^TAG BpuAI 670 GTCTTC GAAGAC(2/6) BsiEI 231 CGGCCG CG[GA][CT]^CG BsmAI 58 GAGAC GTCTC(1/5) BsmFI 447 GTCCC GGGAC(10/14) BsmI 698 GCATTC GAATGC(1/-1) BsrBI 782 CCGCTC CCGCTC(-3/-3) BsrI 666 ACTGG ACTGG(1/-1) Bsu36I 925 CCTGAGG CC^T[ACGT]AGG DraII 440 GGGCCCT [GA]G^G[ACGT]CC[CT] DrdI 733 GACAGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 231 CGGCCG C^GGCCG EarI 220 CTCTTC CTCTTC(1/4) EclXI 231 CGGCCG C^GGCCG Eco47III 356 AGCGCT AGC^GCT Eco52I 231 CGGCCG C^GGCCG Eco81I 925 CCTGAGG CC^T[ACGT]AGG EcoO109I 440 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 20 CCTTGG C^C[AT][AT]GG FbaI 596 TGATCA T^GATCA FspI 167 TGCGCA TGC^GCA HaeII 356 AGCGCT [GA]GCGC^[CT] HapII 618 CCGG C^CGG HindIII 255 AAGCTT A^AGCTT HpaII 618 CCGG C^CGG KpnI 386 GGTACC GGTAC^C Ksp632I 220 CTCTTC CTCTTC(1/4) MaeI 763 CTAG C^TAG MaeIII 646 GTGAC ^GT[ACGT]AC MluI 890 ACGCGT A^CGCGT MspI 618 CCGG C^CGG NciI 617 CCCGG CC^[GC]GG NotI 230 GCGGCCGC GC^GGCCGC NspI 164 GCATGC [GA]CATG^[CT] PleI 60 GACTC GAGTC(4/5) PmaCI 727 CACGTG CAC^GTG PmlI 727 CACGTG CAC^GTG PstI 883 CTGCAG CTGCA^G ScaI 663 AGTACT AGT^ACT SfcI 883 CTGCAG C^T[GA][CT]AG SmlI 81 CTTGAG C^T[CT][GA]AG SphI 164 GCATGC GCATG^C StuI 324 AGGCCT AGG^CCT StyI 20 CCTTGG C^C[AT][AT]GG Tsp45I 646 GTGAC ^GT[GC]AC XcmI 11 CCATCTCAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 174 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AspHI 142 GTGCTC G[AT]GC[AT]^C 348 GAGCAC AvaII 578 GGTCC G^G[AT]CC 588 GGTCC BsaAI 643 TACGTG [CT]AC^GT[GA] 727 CACGTG BsaJI 20 CCTTGG C^C[ACGT][ACGT]GG 153 CCTGGG BseRI 676 CTCCTC GAGGAG(10/8) 55 GAGGAG BsiHKAI 142 GTGCTC G[AT]GC[AT]^C 348 GAGCAC CfoI 168 GCGC GCG^C 357 GCGC EaeI 203 TGGCCG [CT]^GGCC[GA] 231 CGGCCG HhaI 168 GCGC GCG^C 357 GCGC HinP1I 168 GCGC G^CGC 357 GCGC MseI 253 TTAA T^TAA 788 TTAA NlaIII 165 CATG CATG^ 754 CATG SfaNI 104 GCATC GCATC(5/9) 461 GCATC TfiI 505 GATTC G^A[AT]TC 603 GATTC Tru9I 253 TTAA T^TAA 788 TTAA TspRI 495 CAGTG CA[GC]TG[ACGT][ACGT]^ 795 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BamHI BanII BbvI BmyI BpmI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HgaI HinfI ItaI MaeII MboI MboII MflI MnlI MspA1I MvaI MvnI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI TseI XhoII