################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AvrII BalI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI Hin1I HincII HindII HindIII HpaI HphI KasI KspI MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TaqI Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 386 GGTACC G^GTACC Aor51HI 356 AGCGCT AGC^GCT Asp718I 386 GGTACC G^GTACC AspHI 142 GTGCTC G[AT]GC[AT]^C AviII 167 TGCGCA TGC^GCA BanI 386 GGTACC G^G[CT][GA]CC BbsI 670 GTCTTC GAAGAC(2/6) BciVI 461 GTATCC GTATCC(6/5) BcnI 617 CCCGG CC^[GC]GG BfaI 763 CTAG C^TAG BlpI 336 GCTGAGC GC^T[ACGT]AGC Bpu1102I 336 GCTGAGC GC^T[ACGT]AGC BpuAI 670 GTCTTC GAAGAC(2/6) BsaAI 643 TACGTG [CT]AC^GT[GA] BsiEI 209 CGGCCG CG[GA][CT]^CG BsiHKAI 142 GTGCTC G[AT]GC[AT]^C BsmFI 447 GTCCC GGGAC(10/14) BsmI 698 GCATTC GAATGC(1/-1) BsrBI 782 CCGCTC CCGCTC(-3/-3) BsrI 16 CCAGT ACTGG(1/-1) CelII 336 GCTGAGC GC^T[ACGT]AGC EagI 209 CGGCCG C^GGCCG EarI 220 CTCTTC CTCTTC(1/4) EclXI 209 CGGCCG C^GGCCG Eco47III 356 AGCGCT AGC^GCT Eco52I 209 CGGCCG C^GGCCG FspI 167 TGCGCA TGC^GCA HapII 618 CCGG C^CGG HgaI 840 GCGTC GACGC(5/10) HpaII 618 CCGG C^CGG KpnI 386 GGTACC GGTAC^C Ksp632I 220 CTCTTC CTCTTC(1/4) MaeI 763 CTAG C^TAG MseI 788 TTAA T^TAA MspA1I 628 CCGCTG C[AC]G^C[GT]G MspI 618 CCGG C^CGG NciI 617 CCCGG CC^[GC]GG PflMI 368 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 60 GACTC GAGTC(4/5) PstI 883 CTGCAG CTGCA^G SexAI 200 ACCTGGT A^CC[AT]GGT SfcI 883 CTGCAG C^T[GA][CT]AG StuI 324 AGGCCT AGG^CCT Tru9I 788 TTAA T^TAA Van91I 368 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwNI 76 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 853 CAGAACCTG AocI 895 CCTGAGG CC^T[ACGT]AGG 925 CCTCAGG ApaI 440 GGGCCC GGGCC^C 707 GGGCCC AvaII 578 GGTCC G^G[AT]CC 588 GGTCC BamHI 613 GGATCC G^GATCC 722 GGATCC BsaJI 153 CCTGGG C^C[ACGT][ACGT]GG 935 CCCTGG BsmAI 58 GAGAC GTCTC(1/5) 181 GTCTC BstYI 613 GGATCC [GA]^GATC[CT] 722 GGATCC Bsu36I 895 CCTGAGG CC^T[ACGT]AGG 925 CCTCAGG DpnII 614 GATC ^GATC 723 GATC DraII 440 GGGCCCT [GA]G^G[ACGT]CC[CT] 707 GGGCCCT EaeI 209 CGGCCG [CT]^GGCC[GA] 231 CGGCCA Eco81I 895 CCTGAGG CC^T[ACGT]AGG 925 CCTCAGG EcoO109I 440 GGGCCCT [GA]G^G[ACGT]CC[CT] 707 GGGCCCT HaeII 340 AGCGCC [GA]GCGC^[CT] 356 AGCGCT MboI 614 GATC ^GATC 723 GATC MflI 613 GGATCC [GA]^GATC[CT] 722 GGATCC NdeII 614 GATC ^GATC 723 GATC NspI 164 GCATGC [GA]CATG^[CT] 332 GCATGC Sau3AI 614 GATC ^GATC 723 GATC SphI 164 GCATGC GCATG^C 332 GCATGC Tsp45I 482 GTGAC ^GT[GC]AC 646 GTGAC XcmI 11 CCATCCCAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 719 CCAGGATCCACCTGG XhoII 613 GGATCC [GA]^GATC[CT] 722 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BanII BbvI BmyI BpmI BseRI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MaeII MaeIII MboII MnlI MvaI MvnI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI TfiI TseI TspRI