(Circular) (Six-base) MAPSORT of: pFN21AE6035.seq Check: 7256 from: 1 to: 5656 pFN21AE6035 5656 bp With 182 enzymes: SgfI * December 8, 2014 13:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 2482 2582 3719 5103 5371 1632 Size: 512 338 100 1137 1384 268 1917 Fragments arranged by size: 1917 1384 1137 512 338 268 100 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 4555 5532 1203 Size: 3352 977 1327 Fragments arranged by size: 3352 1327 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 2846 1166 Size: 1680 3976 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2411 1184 Size: 1227 4429 AlwNI CAG_nnn'CTG Cuts at: 2946 3399 4971 2946 Size: 453 1572 3631 Fragments arranged by size: 3631 1572 453 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2036 2965 3191 3343 5452 1493 Size: 543 929 226 152 2109 1697 Fragments arranged by size: 2109 1697 929 543 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2981 1870 Size: 73 1038 4545 Fragments arranged by size: 4545 1038 73 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2516 2977 4432 618 Size: 325 217 627 729 461 1455 1842 Fragments arranged by size: 1842 1455 729 627 461 325 217 BanII G_rGCy'C Cuts at: 1391 2975 1391 Size: 1584 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4685 BbvCI CC'TCA_GC Cuts at: 2909 2909 Size: 5656 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2960 3548 4758 2960 Size: 588 1210 3858 Fragments arranged by size: 3858 1210 588 BclI T'GATC_A Cuts at: 1548 1548 Size: 5656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2438 4267 136 Size: 107 122 71 631 605 766 1829 1525 Fragments arranged by size: 1829 1525 766 631 605 122 107 71 BglII A'GATC_T Cuts at: 2595 5651 2595 Size: 3056 2600 BlpI GC'TnA_GC Cuts at: 3073 3073 Size: 5656 Bme1580I G_kGCm'C Cuts at: 1790 2244 3717 4873 1790 Size: 454 1473 1156 2573 Fragments arranged by size: 2573 1473 1156 454 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2399 2948 3387 Size: 416 53 83 399 24 982 549 439 Cuts at: 3387 4345 442 Size: 958 1753 Fragments arranged by size: 1753 982 958 549 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 4316 1239 Size: 995 2082 2579 Fragments arranged by size: 2579 2082 995 Bpu10I CC'TnA_GC Cuts at: 2909 2909 Size: 5656 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2669 2902 3781 4646 4944 2669 Size: 233 879 865 298 3381 Fragments arranged by size: 3381 879 865 298 233 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4319 882 Size: 617 2820 2219 Fragments arranged by size: 2820 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2727 3447 493 Size: 711 1523 720 2702 Fragments arranged by size: 2702 1523 720 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3463 3845 4480 275 Size: 53 83 186 936 1930 382 635 1451 Fragments arranged by size: 1930 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5431 Fragments arranged by size: 5431 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2722 1186 Size: 1536 4120 BseYI C'CCAG_C Cuts at: 2251 4859 5355 2251 Size: 2608 496 2552 Fragments arranged by size: 2608 2552 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2501 2595 1163 Size: 573 765 94 4224 Fragments arranged by size: 4224 765 573 94 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3106 3867 4895 1540 Size: 275 186 1105 761 1028 2301 Fragments arranged by size: 2301 1105 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2732 3717 3802 4873 2732 Size: 985 85 1071 3515 Fragments arranged by size: 3515 1071 985 85 BsmI GAATG_Cn' Cuts at: 2345 2908 3167 3260 2345 Size: 563 259 93 4741 Fragments arranged by size: 4741 563 259 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2244 2732 2975 3717 3802 4873 1391 Size: 399 454 488 243 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 488 454 399 243 85 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 2046 2412 2628 3545 5275 2046 Size: 366 216 917 1730 2427 Fragments arranged by size: 2427 1730 917 366 216 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3011 844 Size: 2167 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3543 1394 Size: 429 1720 3507 Fragments arranged by size: 3507 1720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2081 2667 4151 4325 65 Size: 1183 833 586 1484 174 1396 Fragments arranged by size: 1484 1396 1183 833 586 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4300 4487 1700 Size: 110 2490 187 2869 Fragments arranged by size: 2869 2490 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 3716 4728 3716 Size: 1012 4644 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5656 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 2149 1184 Size: 965 4691 BstXI CCAn_nnnn'nTGG Cuts at: 2251 2623 2251 Size: 372 5284 BstYI r'GATC_y Cuts at: 1972 2595 2688 2986 3353 3738 3755 5207 5293 Size: 623 93 298 367 385 17 1452 86 Cuts at: 5293 5305 5651 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 623 385 367 346 298 93 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2358 513 Size: 552 939 354 3811 Fragments arranged by size: 3811 939 552 354 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4917 Fragments arranged by size: 4917 683 56 BtsI GCAGTG_nn' Cuts at: 3172 3966 3986 3172 Size: 794 20 4842 Fragments arranged by size: 4842 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4663 809 Size: 507 3347 1802 Fragments arranged by size: 3347 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2379 3103 3992 Size: 54 1425 50 275 18 549 724 889 Cuts at: 3992 5621 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 724 549 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3103 1537 Size: 275 1291 4090 Fragments arranged by size: 4090 1291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2062 2093 2501 3586 1353 Size: 709 31 408 1085 3423 Fragments arranged by size: 3423 1085 709 408 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4243 4617 4763 387 Size: 1064 138 2654 374 146 1280 Fragments arranged by size: 2654 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2234 2482 2582 3719 4316 5103 Size: 393 512 90 248 100 1137 597 787 Cuts at: 5103 5371 1239 Size: 268 1524 Fragments arranged by size: 1524 1137 787 597 512 393 268 248 100 90 EcoO109I rG'GnC_Cy Cuts at: 2256 2916 2929 2256 Size: 660 13 4983 Fragments arranged by size: 4983 660 13 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 4162 4162 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 4803 1678 Size: 3125 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373 Size: 32 135 32 2725 32 42 32 2626 Fragments arranged by size: 2725 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2590 4770 4954 1305 Size: 1285 2180 184 2007 Fragments arranged by size: 2180 2007 1285 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2381 10 Size: 54 1425 343 549 3285 Fragments arranged by size: 3285 1425 549 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2306 2621 2846 3614 3973 4132 518 Size: 648 1140 315 225 768 359 159 2042 Fragments arranged by size: 2042 1140 768 648 359 315 225 159 MspA1I CmG'CkG Cuts at: 1606 3072 3749 4897 5142 1606 Size: 1466 677 1148 245 2120 Fragments arranged by size: 2120 1466 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2358 513 Size: 552 939 354 3811 Fragments arranged by size: 3811 939 552 354 NdeI CA'TA_TG Cuts at: 387 2365 387 Size: 1978 3678 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 3014 4559 1132 Size: 97 1785 1545 2229 Fragments arranged by size: 2229 1785 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5656 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 2513 1108 Size: 1405 4251 PfoI T'CCnGG_A Cuts at: 2211 2211 Size: 5656 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3725 3757 5238 5270 3725 Size: 32 1481 32 4111 Fragments arranged by size: 4111 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2256 2916 2929 2256 Size: 660 13 4983 Fragments arranged by size: 4983 660 13 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PstI C_TGCA'G Cuts at: 830 2390 3008 830 Size: 1560 618 3478 Fragments arranged by size: 3478 1560 618 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SanDI GG'GwC_CC Cuts at: 2256 2256 Size: 5656 SapI GCTCTTCn'nnn_ Cuts at: 2093 2501 2093 Size: 408 5248 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 2010 2386 2469 2875 3004 4139 4820 Size: 219 965 376 83 406 129 1135 681 Cuts at: 4820 5011 826 Size: 191 1471 Fragments arranged by size: 1471 1135 965 681 406 376 219 191 129 83 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2684 2881 3760 4661 4923 820 Size: 197 926 741 197 879 901 262 1553 Fragments arranged by size: 1553 926 901 879 741 262 197 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5656 StyI C'CwwG_G Cuts at: 513 1065 2004 2290 2358 3095 513 Size: 552 939 286 68 737 3074 Fragments arranged by size: 3074 939 737 552 286 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3683 3700 3853 4038 4483 325 Size: 1255 2103 17 153 185 445 1498 Fragments arranged by size: 2103 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 3902 5614 Size: 274 80 33 51 1443 207 1718 1712 Cuts at: 5614 96 Size: 138 Fragments arranged by size: 1718 1712 1443 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5616 5648 5616 Size: 32 5624 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 2534 3785 2534 Size: 1251 4405 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI