################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI HaeII Hin1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MseI MspA1I MunI MvnI NarI NciI NdeI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AciI 9 GCGG CCGC(-3/-1) AviII 890 TGCGCA TGC^GCA BanII 911 GAGCCC G[GA]GC[CT]^C BglII 212 AGATCT A^GATCT BmyI 911 GAGCCC G[AGT]GC[ACT]^C BsaI 834 GGTCTC GGTCTC(1/5) Bsp1286I 911 GAGCCC G[AGT]GC[ACT]^C BsrFI 428 GCCGGC [GA]^CCGG[CT] BstXI 824 CCAACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 428 GCCGGC [GA]^CCGG[CT] DraII 658 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 342 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 482 CCATGG C^C[GA][CT]GG EcoO109I 658 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoT22I 303 ATGCAT ATGCA^T FspI 890 TGCGCA TGC^GCA HapII 429 CCGG C^CGG HgaI 713 GACGC GACGC(5/10) HpaII 429 CCGG C^CGG MspI 429 CCGG C^CGG NaeI 428 GCCGGC GCC^GGC NcoI 482 CCATGG C^CATGG NgoMIV 428 GCCGGC G^CCGGC NheI 967 GCTAGC G^CTAGC NsiI 303 ATGCAT ATGCA^T SfcI 70 CTACAG C^T[GA][CT]AG StuI 722 AGGCCT AGG^CCT Tsp509I 147 AATT ^AATT ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BamHI 262 GGATCC G^GATCC 948 GGATCC BpmI 208 CTGGAG CTGGAG(16/14) 464 CTGGAG BsaJI 327 CCTTGG C^C[ACGT][ACGT]GG 482 CCATGG BsiYI 327 CCTTGGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 939 CCAGCACCAGG BslI 327 CCTTGGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 939 CCAGCACCAGG BsmAI 556 GTCTC GTCTC(1/5) 835 GTCTC BsrDI 563 CATTGC GCAATG(2/0) 863 CATTGC BssKI 618 CCTGG ^CC[ACGT]GG 945 CCAGG BstF5I 615 CATCC GGATG(2/0) 301 GGATG BstNI 618 CCTGG CC^[AT]GG 945 CCAGG CfoI 64 GCGC GCG^C 891 GCGC EcoRII 618 CCTGG ^CC[AT]GG 945 CCAGG EcoT14I 327 CCTTGG C^C[AT][AT]GG 482 CCATGG FokI 615 CATCC GGATG(9/13) 301 GGATG HhaI 64 GCGC GCG^C 891 GCGC HinP1I 64 GCGC G^CGC 891 GCGC MvaI 618 CCTGG CC^[AT]GG 945 CCAGG ScrFI 618 CCTGG CC^[ACGT]GG 945 CCAGG StyI 327 CCTTGG C^C[AT][AT]GG 482 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AfaI AluI AlwI BalI BbvI BfaI BsgI BsrI BstYI Cac8I Cfr13I DdeI DpnII EaeI Fnu4HI HaeIII ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MslI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I SfaNI TseI Tsp45I TspRI XhoII