(Circular) (Six-base) MAPSORT of: pFN21AE5474.seq Check: 2999 from: 1 to: 5686 pFN21AE5474 5686 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3496 278 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3029 1937 Size: 1092 4594 Acc65I G'GTAC_C Cuts at: 3007 3007 Size: 5686 AclI AA'CG_TT Cuts at: 1563 3813 4186 1563 Size: 2250 373 3063 Fragments arranged by size: 3063 2250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3749 5133 5401 1632 Size: 2117 1384 268 1917 Fragments arranged by size: 2117 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5489 AflIII A'CryG_T Cuts at: 1203 4585 5562 1203 Size: 3382 977 1327 Fragments arranged by size: 3382 1327 977 AgeI A'CCGG_T Cuts at: 4517 4517 Size: 5686 AhdI GACnn_n'nnGTC Cuts at: 4415 4415 Size: 5686 AleI CACnn'nnGTG Cuts at: 1166 2759 1166 Size: 1593 4093 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2469 1184 Size: 1285 4401 AlwNI CAG_nnn'CTG Cuts at: 3429 5001 3429 Size: 1572 4114 ApaLI G'TGCA_C Cuts at: 3743 4899 3743 Size: 1156 4530 ApoI r'AATT_y Cuts at: 1493 2995 3221 3373 5482 1493 Size: 1502 226 152 2109 1697 Fragments arranged by size: 2109 1697 1502 226 152 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 3011 1870 Size: 73 1068 4545 Fragments arranged by size: 4545 1068 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2072 2105 2072 Size: 33 5653 BamHI G'GATC_C Cuts at: 2267 2953 3016 3383 2267 Size: 686 63 367 4570 Fragments arranged by size: 4570 686 367 63 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3007 4462 618 Size: 325 217 627 1220 1455 1842 Fragments arranged by size: 1842 1455 1220 627 325 217 BanII G_rGCy'C Cuts at: 1391 2920 3005 1391 Size: 1529 85 4072 Fragments arranged by size: 4072 1529 85 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4715 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3877 3911 1903 Size: 34 1940 34 3678 Fragments arranged by size: 3678 1940 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 4788 3578 Size: 1210 4476 BclI T'GATC_A Cuts at: 1548 1548 Size: 5686 BfrBI ATG'CAT Cuts at: 2310 2310 Size: 5686 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4297 136 Size: 107 122 71 631 605 2625 1525 Fragments arranged by size: 2625 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2217 5681 2217 Size: 3464 2222 BlpI GC'TnA_GC Cuts at: 3103 3103 Size: 5686 Bme1580I G_kGCm'C Cuts at: 1790 3747 4903 1790 Size: 1957 1156 2573 Fragments arranged by size: 2573 1957 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5686 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2411 2670 3417 Size: 416 53 83 399 24 994 259 747 Cuts at: 3417 4375 442 Size: 958 1753 Fragments arranged by size: 1753 994 958 747 416 399 259 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2976 1056 Size: 1920 3766 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2490 4346 1239 Size: 995 256 1856 2579 Fragments arranged by size: 2579 1856 995 256 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3811 4676 4974 3811 Size: 865 298 4523 Fragments arranged by size: 4523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2845 4349 882 Size: 617 1346 1504 2219 Fragments arranged by size: 2219 1504 1346 617 BsaAI yAC'GTr Cuts at: 493 1204 3477 493 Size: 711 2273 2702 Fragments arranged by size: 2702 2273 711 BsaBI GATnn'nnATC Cuts at: 5680 5680 Size: 5686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3493 3875 4510 275 Size: 53 83 186 936 1960 382 635 1451 Fragments arranged by size: 1960 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4119 4517 4791 4938 5349 4119 Size: 398 274 147 411 4456 Fragments arranged by size: 4456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2020 2050 2207 2237 1178 Size: 30 165 30 617 30 157 30 4627 Fragments arranged by size: 4627 617 165 157 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5686 BseYI C'CCAG_C Cuts at: 2050 4889 5385 2050 Size: 2839 496 2351 Fragments arranged by size: 2839 2351 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2329 2465 2918 1163 Size: 573 593 136 453 3931 Fragments arranged by size: 3931 593 573 453 136 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3136 3897 4925 1540 Size: 275 186 1135 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3747 3832 4903 3747 Size: 85 1071 4530 Fragments arranged by size: 4530 1071 85 BsmI GAATG_Cn' Cuts at: 3197 3290 3197 Size: 93 5593 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2920 3005 3747 3832 4903 1391 Size: 399 1130 85 742 85 1071 2174 Fragments arranged by size: 2174 1130 1071 742 399 85 85 BspEI T'CCGG_A Cuts at: 5349 5349 Size: 5686 BspHI T'CATG_A Cuts at: 3575 5305 3575 Size: 1730 3956 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3041 844 Size: 2197 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3573 1394 Size: 429 1750 3507 Fragments arranged by size: 3507 1750 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2567 2867 4181 4355 65 Size: 1183 1319 300 1314 174 1396 Fragments arranged by size: 1396 1319 1314 1183 300 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2433 4330 4517 1700 Size: 110 623 1897 187 2869 Fragments arranged by size: 2869 1897 623 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5686 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5686 BssSI C'ACGA_G Cuts at: 3746 4758 3746 Size: 1012 4674 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5686 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5686 BstXI CCAn_nnnn'nTGG Cuts at: 2836 2836 Size: 5686 BstYI r'GATC_y Cuts at: 1972 2217 2267 2953 3016 3383 3768 3785 5237 Size: 245 50 686 63 367 385 17 1452 Cuts at: 5237 5323 5335 5681 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 686 385 367 346 245 86 63 50 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2487 513 Size: 552 939 483 3712 Fragments arranged by size: 3712 939 552 483 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4947 Fragments arranged by size: 4947 683 56 BtsI GCAGTG_nn' Cuts at: 3202 3996 4016 3202 Size: 794 20 4872 Fragments arranged by size: 4872 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5651 DraI TTT'AAA Cuts at: 2990 2990 Size: 5686 DraIII CAC_nnn'GTG Cuts at: 2352 2352 Size: 5686 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4693 809 Size: 507 3377 1802 Fragments arranged by size: 3377 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2391 2431 2625 Size: 54 1425 50 275 18 561 40 194 Cuts at: 2625 2676 2801 3133 4022 5651 8 Size: 51 125 332 889 1629 43 Fragments arranged by size: 1629 1425 889 561 332 275 194 125 54 51 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 3133 1537 Size: 275 1321 4090 Fragments arranged by size: 4090 1321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3616 1353 Size: 2263 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2029 4273 4647 4793 387 Size: 1064 138 440 2244 374 146 1280 Fragments arranged by size: 2244 1280 1064 440 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2490 3749 4346 5133 5401 1239 Size: 393 602 256 1259 597 787 268 1524 Fragments arranged by size: 1524 1259 787 602 597 393 268 256 EcoO109I rG'GnC_Cy Cuts at: 2664 2664 Size: 5686 EcoRI G'AATT_C Cuts at: 2995 3373 2995 Size: 378 5308 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2519 2519 Size: 5686 FspI TGC'GCA Cuts at: 2897 4192 2897 Size: 1295 4391 HaeII r_GCGC'y Cuts at: 1678 4833 1678 Size: 3155 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2277 2309 4327 4359 4401 Size: 32 135 32 705 32 2018 32 42 Cuts at: 4401 4433 1373 Size: 32 2626 Fragments arranged by size: 2626 2018 705 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3030 3276 669 Size: 988 281 1092 246 3079 Fragments arranged by size: 3079 1092 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5686 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KpnI G_GTAC'C Cuts at: 3011 3011 Size: 5686 MfeI C'AATT_G Cuts at: 3285 3285 Size: 5686 MluI A'CGCG_T Cuts at: 5562 5562 Size: 5686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2245 4800 4984 1305 Size: 940 2555 184 2007 Fragments arranged by size: 2555 2007 940 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2393 2627 2678 10 Size: 54 1425 343 561 234 51 3018 Fragments arranged by size: 3018 1425 561 343 234 54 51 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2486 2591 2759 3644 4003 4162 Size: 648 978 342 105 168 885 359 159 Cuts at: 4162 518 Size: 2042 Fragments arranged by size: 2042 978 885 648 359 342 168 159 105 MspA1I CmG'CkG Cuts at: 1606 3102 3779 4927 5172 1606 Size: 1496 677 1148 245 2120 Fragments arranged by size: 2120 1496 1148 677 245 NaeI GCC'GGC Cuts at: 2435 2435 Size: 5686 NcoI C'CATG_G Cuts at: 513 1065 2004 2487 513 Size: 552 939 483 3712 Fragments arranged by size: 3712 939 552 483 NdeI CA'TA_TG Cuts at: 387 387 Size: 5686 NgoMIV G'CCGG_C Cuts at: 2433 2433 Size: 5686 NheI G'CTAG_C Cuts at: 1052 2972 1052 Size: 1920 3766 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NsiI A_TGCA'T Cuts at: 2312 2312 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 3044 4589 1132 Size: 97 1815 1545 2229 Fragments arranged by size: 2229 1815 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5686 PciI A'CATG_T Cuts at: 4585 4585 Size: 5686 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4784 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 5268 5300 3755 Size: 32 1481 32 4141 Fragments arranged by size: 4141 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5686 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PstI C_TGCA'G Cuts at: 830 3038 830 Size: 2208 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SbfI CC_TGCA'GG Cuts at: 3038 3038 Size: 5686 ScaI AGT'ACT Cuts at: 3934 5646 3934 Size: 1712 3974 SfcI C'TryA_G Cuts at: 826 1045 2075 3034 4169 4850 5041 826 Size: 219 1030 959 1135 681 191 1471 Fragments arranged by size: 1471 1135 1030 959 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5686 SmaI CCC'GGG Cuts at: 1872 3013 1872 Size: 1141 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3790 4691 4953 820 Size: 197 926 1847 901 262 1553 Fragments arranged by size: 1847 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 3044 1132 Size: 1912 3774 SspI AAT'ATT Cuts at: 5 52 3610 5 Size: 47 3558 2081 Fragments arranged by size: 3558 2081 47 StuI AGG'CCT Cuts at: 1517 2729 1517 Size: 1212 4474 StyI C'CwwG_G Cuts at: 513 1065 2004 2332 2487 3125 513 Size: 552 939 328 155 638 3074 Fragments arranged by size: 3074 939 638 552 328 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3713 3730 3883 4068 4513 325 Size: 1255 2133 17 153 185 445 1498 Fragments arranged by size: 2133 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 2845 2927 Size: 274 80 33 51 1443 207 661 82 Cuts at: 2927 3932 5644 96 Size: 1005 1712 138 Fragments arranged by size: 1712 1443 1005 661 274 207 138 82 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2008 2020 2040 2052 5646 5678 2008 Size: 12 20 12 3594 32 2016 Fragments arranged by size: 3594 2016 32 20 12 12 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5686 XmaI C'CCGG_G Cuts at: 1870 3011 1870 Size: 1141 4545 XmnI GAAnn'nnTTC Cuts at: 3815 3815 Size: 5686 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3494 276 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI