(Linear) (Six-base) MAPSORT of: KIEE5474.seq Check: 7558 from: 1 to: 981 KIEE5474.orf With 182 enzymes: SgfI * January 29, 2014 12:02 .. AleI CACnn'nnGTG Cuts at: 0 754 981 Size: 754 227 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 464 981 Size: 464 517 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 67 100 981 Size: 67 33 881 Fragments arranged by size: 881 67 33 BamHI G'GATC_C Cuts at: 0 262 948 981 Size: 262 686 33 Fragments arranged by size: 686 262 33 BanII G_rGCy'C Cuts at: 0 915 981 Size: 915 66 BfrBI ATG'CAT Cuts at: 0 305 981 Size: 305 676 BglII A'GATC_T Cuts at: 0 212 981 Size: 212 769 BmrI ACTGGGnnnn_n' Cuts at: 0 406 665 981 Size: 406 259 316 Fragments arranged by size: 406 316 259 BmtI G_CTAG'C Cuts at: 0 971 981 Size: 971 10 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 485 981 Size: 229 256 496 Fragments arranged by size: 496 256 229 BsaI GGTCTCn'nnnn_ Cuts at: 0 840 981 Size: 840 141 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 15 45 202 232 981 Size: 15 30 157 30 749 Fragments arranged by size: 749 157 30 30 15 BseYI C'CCAG_C Cuts at: 0 45 981 Size: 45 936 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 324 460 913 981 Size: 324 136 453 68 Fragments arranged by size: 453 324 136 68 Bsp1286I G_dGCh'C Cuts at: 0 915 981 Size: 915 66 BsrDI GCAATG_nn' Cuts at: 0 562 862 981 Size: 562 300 119 Fragments arranged by size: 562 300 119 BsrFI r'CCGG_y Cuts at: 0 428 981 Size: 428 553 BstXI CCAn_nnnn'nTGG Cuts at: 0 831 981 Size: 831 150 BstYI r'GATC_y Cuts at: 0 212 262 948 981 Size: 212 50 686 33 Fragments arranged by size: 686 212 50 33 BtgI C'CryG_G Cuts at: 0 482 981 Size: 482 499 DraIII CAC_nnn'GTG Cuts at: 0 347 981 Size: 347 634 EaeI y'GGCC_r Cuts at: 0 386 426 620 671 796 981 Size: 386 40 194 51 125 185 Fragments arranged by size: 386 194 185 125 51 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 24 981 Size: 24 957 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 485 981 Size: 229 256 496 Fragments arranged by size: 496 256 229 EcoO109I rG'GnC_Cy Cuts at: 0 659 981 Size: 659 322 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 514 981 Size: 514 467 FspI TGC'GCA Cuts at: 0 892 981 Size: 892 89 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 272 304 981 Size: 272 32 677 Fragments arranged by size: 677 272 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 240 981 Size: 240 741 MscI TGG'CCA Cuts at: 0 388 622 673 981 Size: 388 234 51 308 Fragments arranged by size: 388 308 234 51 MslI CAynn'nnrTG Cuts at: 0 139 481 586 754 981 Size: 139 342 105 168 227 Fragments arranged by size: 342 227 168 139 105 NaeI GCC'GGC Cuts at: 0 430 981 Size: 430 551 NcoI C'CATG_G Cuts at: 0 482 981 Size: 482 499 NgoMIV G'CCGG_C Cuts at: 0 428 981 Size: 428 553 NheI G'CTAG_C Cuts at: 0 967 981 Size: 967 14 NsiI A_TGCA'T Cuts at: 0 307 981 Size: 307 674 SfcI C'TryA_G Cuts at: 0 70 981 Size: 70 911 StuI AGG'CCT Cuts at: 0 724 981 Size: 724 257 StyI C'CwwG_G Cuts at: 0 327 482 981 Size: 327 155 499 Fragments arranged by size: 499 327 155 TatI w'GTAC_w Cuts at: 0 179 840 922 981 Size: 179 661 82 59 Fragments arranged by size: 661 179 82 59 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3 15 35 47 981 Size: 3 12 20 12 934 Fragments arranged by size: 934 20 12 12 3 Enzymes that do cut: AleI AlfI BaeI BamHI BanII BfrBI BglII BmrI BmtI BpmI BsaI BsaXI BseYI BsgI Bsp1286I BsrDI BsrFI BstXI BstYI BtgI DraIII EaeI EciI Eco57MI EcoO109I FalI FspI Hin4I MmeI MscI MslI NaeI NcoI NgoMIV NheI NsiI SfcI StuI StyI TatI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BanI BbeI BbsI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MspA1I NarI NdeI NotI NruI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI