################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MscI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 479 TCCGGA T^CCGGA AhdI 856 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 856 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1018 GAGCAC G[AT]GC[AT]^C BanII 892 GGGCTC G[GA]GC[CT]^C BbsI 1063 GTCTTC GAAGAC(2/6) BglII 314 AGATCT A^GATCT BpuAI 1063 GTCTTC GAAGAC(2/6) BsaI 1128 GGTCTC GGTCTC(1/5) BsaWI 479 TCCGGA [AT]^CCGG[AT] BseAI 479 TCCGGA T^CCGGA BsiHKAI 1018 GAGCAC G[AT]GC[AT]^C BsmBI 342 GAGACG CGTCTC(1/5) BspEI 479 TCCGGA T^CCGGA BspHI 773 TCATGA T^CATGA BsrFI 129 GCCGGT [GA]^CCGG[CT] BstYI 314 AGATCT [GA]^GATC[CT] Cfr10I 129 GCCGGT [GA]^CCGG[CT] DpnII 315 GATC ^GATC Eam1105I 856 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 450 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 681 CCCGC CCCGC(4/6) HinfI 111 GAGTC G^A[ACGT]TC MboI 315 GATC ^GATC MflI 314 AGATCT [GA]^GATC[CT] MroI 479 TCCGGA T^CCGGA NdeII 315 GATC ^GATC PleI 111 GAGTC GAGTC(4/5) PpuMI 848 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 525 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 773 TCATGA T^CATGA SapI 1038 GAAGAGC GCTCTTC(1/4) Sau3AI 315 GATC ^GATC SbfI 1086 CCTGCAGG CCTGCA^GG SmlI 184 CTTGAG C^T[CT][GA]AG SphI 417 GCATGC GCATG^C Sse8387I 1086 CCTGCAGG CCTGCA^GG StuI 1010 AGGCCT AGG^CCT XcmI 549 CCAGCATTTCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 314 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 604 CGCG CG^CG 918 CGCG BlpI 516 GCTGAGC GC^T[ACGT]AGC 591 GCTAAGC BmyI 892 GGGCTC G[AGT]GC[ACT]^C 1018 GAGCAC BpmI 103 CTGGAG CTGGAG(16/14) 1099 CTGGAG Bpu1102I 516 GCTGAGC GC^T[ACGT]AGC 591 GCTAAGC BseRI 19 CTCCTC GAGGAG(10/8) 511 GAGGAG BsgI 430 CTGCAC GTGCAG(16/14) 646 CTGCAC BsmAI 342 GAGAC GTCTC(1/5) 1129 GTCTC Bsp1286I 892 GGGCTC G[AGT]GC[ACT]^C 1018 GAGCAC BspMI 1172 ACCTGC ACCTGC(4/8) 1089 GCAGGT BsrI 243 ACTGG ACTGG(1/-1) 461 ACTGG BstAPI 194 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 432 GCACATAGTGC BstUI 604 CGCG CG^CG 918 CGCG CelII 516 GCTGAGC GC^T[ACGT]AGC 591 GCTAAGC CfoI 603 GCGC GCG^C 796 GCGC DraIII 379 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG 433 CACATAGTG EaeI 348 TGGCCG [CT]^GGCC[GA] 635 TGGCCG HgaI 96 GACGC GACGC(5/10) 842 GCGTC HhaI 603 GCGC GCG^C 796 GCGC HinP1I 603 GCGC G^CGC 796 GCGC MslI 553 CATTTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 615 CATCCACATG MvnI 604 CGCG CG^CG 918 CGCG NcoI 259 CCATGG C^CATGG 270 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaI AvaII BbvI BcnI BsaJI BsiYI BslI BsmFI BsoBI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII HphI ItaI Ksp632I MaeII MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV NspI PflMI PstI PvuII RsaI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XmaCI XmaI