(Circular) (Six-base) MAPSORT of: pFN21AE5532.seq Check: 4869 from: 1 to: 5884 pFN21AE5532 5884 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3085 3085 Size: 5884 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3694 278 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3227 1937 Size: 1290 4594 Acc65I G'GTAC_C Cuts at: 3205 3205 Size: 5884 AclI AA'CG_TT Cuts at: 1563 4011 4384 1563 Size: 2448 373 3063 Fragments arranged by size: 3063 2448 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2363 2999 3104 3947 5331 5599 1632 Size: 731 636 105 843 1384 268 1917 Fragments arranged by size: 1917 1384 843 731 636 268 105 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5687 AflIII A'CryG_T Cuts at: 1203 4783 5760 1203 Size: 3580 977 1327 Fragments arranged by size: 3580 1327 977 AgeI A'CCGG_T Cuts at: 4715 4715 Size: 5884 AhdI GACnn_n'nnGTC Cuts at: 2866 4613 2866 Size: 1747 4137 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5884 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5884 AlwNI CAG_nnn'CTG Cuts at: 2205 2857 2977 3160 3627 5199 2205 Size: 652 120 183 467 1572 2890 Fragments arranged by size: 2890 1572 652 467 183 120 ApaLI G'TGCA_C Cuts at: 3941 5097 3941 Size: 1156 4728 ApoI r'AATT_y Cuts at: 1493 3193 3419 3571 5680 1493 Size: 1700 226 152 2109 1697 Fragments arranged by size: 2109 1700 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4438 160 Size: 872 3406 1606 Fragments arranged by size: 3406 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 AvaI C'yCGr_G Cuts at: 1870 1943 2220 2702 3114 3209 1870 Size: 73 277 482 412 95 4545 Fragments arranged by size: 4545 482 412 277 95 73 BamHI G'GATC_C Cuts at: 3214 3581 3214 Size: 367 5517 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3205 4660 618 Size: 325 217 627 1418 1455 1842 Fragments arranged by size: 1842 1455 1418 627 325 217 BanII G_rGCy'C Cuts at: 1391 2901 3203 1391 Size: 1510 302 4072 Fragments arranged by size: 4072 1510 302 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3061 928 Size: 971 1162 3751 Fragments arranged by size: 3751 1162 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4075 4109 1903 Size: 34 50 34 2054 34 3678 Fragments arranged by size: 3678 2054 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3776 4986 3776 Size: 1210 4674 BclI T'GATC_A Cuts at: 1548 1548 Size: 5884 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4495 136 Size: 107 122 71 631 605 2823 1525 Fragments arranged by size: 2823 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2319 5879 2319 Size: 3560 2324 BlpI GC'TnA_GC Cuts at: 2522 2597 3301 2522 Size: 75 704 5105 Fragments arranged by size: 5105 704 75 Bme1580I G_kGCm'C Cuts at: 1790 3945 5101 1790 Size: 2155 1156 2573 Fragments arranged by size: 2573 2155 1156 BmgBI CAC'GTC Cuts at: 1318 2351 2389 3064 1318 Size: 1033 38 675 4138 Fragments arranged by size: 4138 1033 675 38 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2258 3615 4573 Size: 416 53 83 399 24 841 1357 958 Cuts at: 4573 442 Size: 1753 Fragments arranged by size: 1753 1357 958 841 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5884 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 3125 4544 1239 Size: 890 996 1419 2579 Fragments arranged by size: 2579 1419 996 890 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2210 4009 4874 5172 2210 Size: 1799 865 298 2922 Fragments arranged by size: 2922 1799 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3139 4547 882 Size: 617 1640 1408 2219 Fragments arranged by size: 2219 1640 1408 617 BsaAI yAC'GTr Cuts at: 493 1204 3675 493 Size: 711 2471 2702 Fragments arranged by size: 2702 2471 711 BsaBI GATnn'nnATC Cuts at: 5878 5878 Size: 5884 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3691 4073 4708 275 Size: 53 83 186 936 2158 382 635 1451 Fragments arranged by size: 2158 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2484 4317 4715 4989 5136 5547 2484 Size: 1833 398 274 147 411 2821 Fragments arranged by size: 2821 1833 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2912 2942 1178 Size: 30 165 30 1509 30 4120 Fragments arranged by size: 4120 1509 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 2531 1186 Size: 829 516 4539 Fragments arranged by size: 4539 829 516 BseYI C'CCAG_C Cuts at: 2237 2716 3121 3148 5087 5583 2237 Size: 479 405 27 1939 496 2538 Fragments arranged by size: 2538 1939 496 479 405 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2420 2636 1163 Size: 573 684 216 4411 Fragments arranged by size: 4411 684 573 216 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3334 4095 5123 1540 Size: 275 186 1333 761 1028 2301 Fragments arranged by size: 2301 1333 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3027 3945 4030 5101 3027 Size: 918 85 1071 3810 Fragments arranged by size: 3810 1071 918 85 BsmI GAATG_Cn' Cuts at: 3395 3488 3395 Size: 93 5791 BsmBI CGTCTCn'nnnn_ Cuts at: 2341 2341 Size: 5884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2901 3027 3203 3945 4030 5101 1391 Size: 399 1111 126 176 742 85 1071 2174 Fragments arranged by size: 2174 1111 1071 742 399 176 126 85 BspEI T'CCGG_A Cuts at: 2484 5547 2484 Size: 3063 2821 BspHI T'CATG_A Cuts at: 2778 3773 5503 2778 Size: 995 1730 3159 Fragments arranged by size: 3159 1730 995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3085 3186 3239 844 Size: 2241 101 53 3489 Fragments arranged by size: 3489 2241 101 53 BsrBI CCG'CTC Cuts at: 1394 1823 3771 1394 Size: 429 1948 3507 Fragments arranged by size: 3507 1948 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4379 4553 65 Size: 1183 3131 174 1396 Fragments arranged by size: 3131 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2134 4528 4715 1700 Size: 110 324 2394 187 2869 Fragments arranged by size: 2869 2394 324 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5884 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5884 BssSI C'ACGA_G Cuts at: 3944 4956 3944 Size: 1012 4872 BstAPI GCAn_nnn'nTGC Cuts at: 794 2205 2443 794 Size: 1411 238 4235 Fragments arranged by size: 4235 1411 238 BstBI TT'CG_AA Cuts at: 4688 4688 Size: 5884 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5884 BstYI r'GATC_y Cuts at: 1972 2319 3214 3581 3966 3983 5435 5521 5533 Size: 347 895 367 385 17 1452 86 12 Cuts at: 5533 5879 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 895 385 367 347 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2264 2275 2532 513 Size: 552 1199 11 257 3865 Fragments arranged by size: 3865 1199 552 257 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2180 487 Size: 683 56 954 4191 Fragments arranged by size: 4191 954 683 56 BtsI GCAGTG_nn' Cuts at: 3058 3400 4194 4214 3058 Size: 342 794 20 4728 Fragments arranged by size: 4728 794 342 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5884 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5849 DraI TTT'AAA Cuts at: 3188 3188 Size: 5884 DraIII CAC_nnn'GTG Cuts at: 2389 2443 2389 Size: 54 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4891 809 Size: 507 3575 1802 Fragments arranged by size: 3575 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2353 2640 3331 Size: 54 1425 50 275 18 523 287 691 Cuts at: 3331 4220 5849 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 691 523 287 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3331 1537 Size: 275 1519 4090 Fragments arranged by size: 4090 1519 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 2108 2339 3038 3814 1353 Size: 668 87 231 699 776 3423 Fragments arranged by size: 3423 776 699 668 231 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4471 4845 4991 387 Size: 1064 138 2882 374 146 1280 Fragments arranged by size: 2882 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2363 2999 3104 3125 3947 4544 Size: 393 497 234 636 105 21 822 597 Cuts at: 4544 5331 5599 1239 Size: 787 268 1524 Fragments arranged by size: 1524 822 787 636 597 497 393 268 234 105 21 EcoNI CCTnn'n_nnAGG Cuts at: 2459 2459 Size: 5884 EcoO109I rG'GnC_Cy Cuts at: 2431 2854 2991 3157 2431 Size: 423 137 166 5158 Fragments arranged by size: 5158 423 166 137 EcoRI G'AATT_C Cuts at: 3193 3571 3193 Size: 378 5506 FspI TGC'GCA Cuts at: 4390 4390 Size: 5884 HaeII r_GCGC'y Cuts at: 1678 5031 1678 Size: 3353 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2832 2852 2864 2884 4525 Size: 32 135 32 1260 20 12 20 1641 Cuts at: 4525 4557 4599 4631 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1641 1260 135 42 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 3228 3474 669 Size: 988 281 1290 246 3079 Fragments arranged by size: 3079 1290 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5884 HpaI GTT'AAC Cuts at: 3474 3474 Size: 5884 KpnI G_GTAC'C Cuts at: 3209 3209 Size: 5884 MfeI C'AATT_G Cuts at: 3483 3483 Size: 5884 MluI A'CGCG_T Cuts at: 5760 5760 Size: 5884 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4998 5182 1305 Size: 3693 184 2007 Fragments arranged by size: 3693 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4062 Fragments arranged by size: 4062 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2562 2624 3842 4201 4360 518 Size: 648 1396 62 1218 359 159 2042 Fragments arranged by size: 2042 1396 1218 648 359 159 62 MspA1I CmG'CkG Cuts at: 1606 2341 2593 2716 2749 3300 3977 5125 5370 Size: 735 252 123 33 551 677 1148 245 Cuts at: 5370 1606 Size: 2120 Fragments arranged by size: 2120 1148 735 677 551 252 245 123 33 NcoI C'CATG_G Cuts at: 513 1065 2264 2275 513 Size: 552 1199 11 4122 Fragments arranged by size: 4122 1199 552 11 NdeI CA'TA_TG Cuts at: 387 387 Size: 5884 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5884 NotI GC'GGCC_GC Cuts at: 3331 3331 Size: 5884 NspI r_CATG'y Cuts at: 1132 1229 2426 2629 2725 3242 4787 1132 Size: 97 1197 203 96 517 1545 2229 Fragments arranged by size: 2229 1545 1197 517 203 97 96 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5884 PciI A'CATG_T Cuts at: 4783 4783 Size: 5884 PflMI CCAn_nnn'nTGG Cuts at: 1108 2389 2857 3160 1108 Size: 1281 468 303 3832 Fragments arranged by size: 3832 1281 468 303 PfoI T'CCnGG_A Cuts at: 2856 2856 Size: 5884 PmeI GTTT'AAAC Cuts at: 3188 3188 Size: 5884 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3953 3985 5466 5498 3953 Size: 32 1481 32 4339 Fragments arranged by size: 4339 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2854 2854 Size: 5884 PshAI GACnn'nnGTC Cuts at: 1613 2534 1613 Size: 921 4963 PsiI TTA'TAA Cuts at: 3454 3454 Size: 5884 PstI C_TGCA'G Cuts at: 830 2048 2340 3096 3236 830 Size: 1218 292 756 140 3478 Fragments arranged by size: 3478 1218 756 292 140 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5884 PvuII CAG'CTG Cuts at: 2341 2593 2716 2749 2341 Size: 252 123 33 5476 Fragments arranged by size: 5476 252 123 33 SalI G'TCGA_C Cuts at: 1936 3226 1936 Size: 1290 4594 SapI GCTCTTCn'nnn_ Cuts at: 3038 3038 Size: 5884 SbfI CC_TGCA'GG Cuts at: 3096 3236 3096 Size: 140 5744 ScaI AGT'ACT Cuts at: 4132 5844 4132 Size: 1712 4172 SfcI C'TryA_G Cuts at: 826 1045 2044 2336 2796 3092 3232 4367 5048 Size: 219 999 292 460 296 140 1135 681 Cuts at: 5048 5239 826 Size: 191 1471 Fragments arranged by size: 1471 1135 999 681 460 296 292 219 191 140 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5884 SmaI CCC'GGG Cuts at: 1872 2222 2704 3116 3211 1872 Size: 350 482 412 95 4545 Fragments arranged by size: 4545 482 412 350 95 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 3988 4889 5151 820 Size: 197 926 246 1799 901 262 1553 Fragments arranged by size: 1799 1553 926 901 262 246 197 SnaBI TAC'GTA Cuts at: 493 3675 493 Size: 3182 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5884 SphI G_CATG'C Cuts at: 1132 2426 3242 1132 Size: 1294 816 3774 Fragments arranged by size: 3774 1294 816 SspI AAT'ATT Cuts at: 5 52 3808 5 Size: 47 3756 2081 Fragments arranged by size: 3756 2081 47 StuI AGG'CCT Cuts at: 1517 3017 1517 Size: 1500 4384 StyI C'CwwG_G Cuts at: 513 1065 2264 2275 2292 2395 2427 2931 3323 Size: 552 1199 11 17 103 32 504 392 Cuts at: 3323 513 Size: 3074 Fragments arranged by size: 3074 1199 552 504 392 103 32 17 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2648 2723 3911 3928 4081 4266 4711 Size: 1255 1068 75 1188 17 153 185 445 Cuts at: 4711 325 Size: 1498 Fragments arranged by size: 1498 1255 1188 1068 445 185 153 75 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2245 3036 4130 Size: 274 80 33 51 1443 268 791 1094 Cuts at: 4130 5842 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1094 791 274 268 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2384 2416 5844 5876 2384 Size: 32 3428 32 2392 Fragments arranged by size: 3428 2392 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5884 XbaI T'CTAG_A Cuts at: 3220 3220 Size: 5884 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2561 2561 Size: 5884 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5884 XmaI C'CCGG_G Cuts at: 1870 2220 2702 3114 3209 1870 Size: 350 482 412 95 4545 Fragments arranged by size: 4545 482 412 350 95 XmnI GAAnn'nnTTC Cuts at: 4013 4013 Size: 5884 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3692 276 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI