(Circular) (Six-base) MAPSORT of: pFN21AE5659.seq Check: 2049 from: 1 to: 6184 pFN21AE5659 6184 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2068 2068 Size: 6184 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3994 278 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3527 1937 Size: 1590 4594 Acc65I G'GTAC_C Cuts at: 3505 3505 Size: 6184 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2745 4247 5631 5899 1632 Size: 1113 1502 1384 268 1917 Fragments arranged by size: 1917 1502 1384 1113 268 AfeI AGC'GCT Cuts at: 2192 2271 2849 2192 Size: 79 578 5527 Fragments arranged by size: 5527 578 79 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5987 AflIII A'CryG_T Cuts at: 1203 5083 6060 1203 Size: 3880 977 1327 Fragments arranged by size: 3880 1327 977 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 4913 4913 Size: 6184 AleI CACnn'nnGTG Cuts at: 1166 3092 1166 Size: 1926 4258 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6184 AlwNI CAG_nnn'CTG Cuts at: 2091 3927 5499 2091 Size: 1836 1572 2776 Fragments arranged by size: 2776 1836 1572 ApaLI G'TGCA_C Cuts at: 2081 4241 5397 2081 Size: 2160 1156 2868 Fragments arranged by size: 2868 2160 1156 ApoI r'AATT_y Cuts at: 1493 3209 3493 3719 3871 5980 1493 Size: 1716 284 226 152 2109 1697 Fragments arranged by size: 2109 1716 1697 284 226 152 AseI AT'TA_AT Cuts at: 160 1032 4738 160 Size: 872 3706 1606 Fragments arranged by size: 3706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 2296 2790 3123 3509 1870 Size: 73 353 494 333 386 4545 Fragments arranged by size: 4545 494 386 353 333 73 BamHI G'GATC_C Cuts at: 2442 3514 3881 2442 Size: 1072 367 4745 Fragments arranged by size: 4745 1072 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3505 4960 618 Size: 325 217 627 1718 1455 1842 Fragments arranged by size: 1842 1718 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2225 2940 3503 1391 Size: 834 715 563 4072 Fragments arranged by size: 4072 834 715 563 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2489 928 Size: 971 590 4623 Fragments arranged by size: 4623 971 590 BbvCI CC'TCA_GC Cuts at: 3416 3416 Size: 6184 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4375 4409 1903 Size: 34 2438 34 3678 Fragments arranged by size: 3678 2438 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3137 3455 4076 5286 3137 Size: 318 621 1210 4035 Fragments arranged by size: 4035 1210 621 318 BclI T'GATC_A Cuts at: 1548 1548 Size: 6184 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4795 136 Size: 107 122 71 631 605 3123 1525 Fragments arranged by size: 3123 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6179 6179 Size: 6184 BlpI GC'TnA_GC Cuts at: 3601 3601 Size: 6184 Bme1580I G_kGCm'C Cuts at: 1790 2085 3099 3164 4245 5401 1790 Size: 295 1014 65 1081 1156 2573 Fragments arranged by size: 2573 1156 1081 1014 295 65 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6184 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3915 4873 442 Size: 416 53 83 399 24 2498 958 1753 Fragments arranged by size: 2498 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6184 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2327 3164 2327 Size: 837 5347 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2633 3339 4844 1239 Size: 1394 706 1505 2579 Fragments arranged by size: 2579 1505 1394 706 Bpu10I CC'TnA_GC Cuts at: 3416 3416 Size: 6184 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4309 5174 5472 4309 Size: 865 298 5021 Fragments arranged by size: 5021 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2903 4847 882 Size: 617 1404 1944 2219 Fragments arranged by size: 2219 1944 1404 617 BsaAI yAC'GTr Cuts at: 493 1204 2218 3975 493 Size: 711 1014 1757 2702 Fragments arranged by size: 2702 1757 1014 711 BsaBI GATnn'nnATC Cuts at: 6178 6178 Size: 6184 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3991 4373 5008 275 Size: 53 83 186 936 2458 382 635 1451 Fragments arranged by size: 2458 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2403 4617 5015 5289 5436 5847 2403 Size: 2214 398 274 147 411 2740 Fragments arranged by size: 2740 2214 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2010 2040 1178 Size: 30 165 30 607 30 5322 Fragments arranged by size: 5322 607 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6184 BseYI C'CCAG_C Cuts at: 2187 2669 5387 5883 2187 Size: 482 2718 496 2488 Fragments arranged by size: 2718 2488 496 482 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2862 1163 Size: 573 1126 4485 Fragments arranged by size: 4485 1126 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3634 4395 5423 1540 Size: 275 186 1633 761 1028 2301 Fragments arranged by size: 2301 1633 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2085 2763 4245 4330 5401 2085 Size: 678 1482 85 1071 2868 Fragments arranged by size: 2868 1482 1071 678 85 BsmI GAATG_Cn' Cuts at: 3695 3788 3695 Size: 93 6091 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2225 2763 2940 3099 3164 3503 Size: 399 295 140 538 177 159 65 339 Cuts at: 3503 4245 4330 5401 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 538 399 339 295 177 159 140 85 65 BspEI T'CCGG_A Cuts at: 2403 5847 2403 Size: 3444 2740 BspHI T'CATG_A Cuts at: 2949 4073 5803 2949 Size: 1124 1730 3330 Fragments arranged by size: 3330 1730 1124 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2068 2371 3114 3539 844 Size: 1224 303 743 425 3489 Fragments arranged by size: 3489 1224 743 425 303 BsrBI CCG'CTC Cuts at: 1394 1823 3480 4071 1394 Size: 429 1657 591 3507 Fragments arranged by size: 3507 1657 591 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2437 4679 4853 65 Size: 1183 1189 2242 174 1396 Fragments arranged by size: 2242 1396 1189 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2200 4828 5015 1700 Size: 110 390 2628 187 2869 Fragments arranged by size: 2869 2628 390 187 110 BsrGI T'GTAC_A Cuts at: 96 2853 96 Size: 2757 3427 BssHII G'CGCG_C Cuts at: 1925 2526 1925 Size: 601 5583 BssSI C'ACGA_G Cuts at: 2813 3092 4244 5256 2813 Size: 279 1152 1012 3741 Fragments arranged by size: 3741 1152 1012 279 BstAPI GCAn_nnn'nTGC Cuts at: 794 3043 794 Size: 2249 3935 BstBI TT'CG_AA Cuts at: 4988 4988 Size: 6184 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6184 BstYI r'GATC_y Cuts at: 1972 2442 3514 3881 4266 4283 5735 5821 5833 Size: 470 1072 367 385 17 1452 86 12 Cuts at: 5833 6179 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1072 470 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2025 513 Size: 552 960 4672 Fragments arranged by size: 4672 960 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5445 Fragments arranged by size: 5445 683 56 BtsI GCAGTG_nn' Cuts at: 3700 4494 4514 3700 Size: 794 20 5370 Fragments arranged by size: 5370 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6149 DraI TTT'AAA Cuts at: 3488 3488 Size: 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5191 809 Size: 507 3875 1802 Fragments arranged by size: 3875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2883 3631 4520 Size: 54 1425 50 275 18 1053 748 889 Cuts at: 4520 6149 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1053 889 748 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3631 1537 Size: 275 1819 4090 Fragments arranged by size: 4090 1819 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2144 2561 2964 4114 1353 Size: 791 417 403 1150 3423 Fragments arranged by size: 3423 1150 791 417 403 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4771 5145 5291 387 Size: 1064 138 3182 374 146 1280 Fragments arranged by size: 3182 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2633 2745 3339 4247 4844 5631 5899 Size: 393 1001 112 594 908 597 787 268 Cuts at: 5899 1239 Size: 1524 Fragments arranged by size: 1524 1001 908 787 597 594 393 268 112 EcoO109I rG'GnC_Cy Cuts at: 2108 2794 2108 Size: 686 5498 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 EcoRV GAT'ATC Cuts at: 3409 3409 Size: 6184 FspI TGC'GCA Cuts at: 2328 4690 2328 Size: 2362 3822 FspAI rTGC'GCAy Cuts at: 2328 2328 Size: 6184 HaeII r_GCGC'y Cuts at: 1678 2194 2273 2851 5331 1678 Size: 516 79 578 2480 2531 Fragments arranged by size: 2531 2480 578 516 79 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2279 2311 2726 2758 2922 Size: 32 135 32 707 32 415 32 164 Cuts at: 2922 2954 3135 3167 4825 4857 4899 4931 1373 Size: 32 181 32 1658 32 42 32 2626 Fragments arranged by size: 2626 1658 707 415 181 164 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3397 3528 3774 669 Size: 988 281 1459 131 246 3079 Fragments arranged by size: 3079 1459 988 281 246 131 HindIII A'AGCT_T Cuts at: 748 748 Size: 6184 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KpnI G_GTAC'C Cuts at: 3509 3509 Size: 6184 MfeI C'AATT_G Cuts at: 3783 3783 Size: 6184 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2127 3126 5298 5482 1305 Size: 822 999 2172 184 2007 Fragments arranged by size: 2172 2007 999 822 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4362 Fragments arranged by size: 4362 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 3092 4142 4501 4660 518 Size: 648 1071 855 1050 359 159 2042 Fragments arranged by size: 2042 1071 1050 855 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2091 3079 3600 4277 5425 5670 1606 Size: 485 988 521 677 1148 245 2120 Fragments arranged by size: 2120 1148 988 677 521 485 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5632 NdeI CA'TA_TG Cuts at: 387 387 Size: 6184 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6184 NotI GC'GGCC_GC Cuts at: 3631 3631 Size: 6184 NspI r_CATG'y Cuts at: 1132 1229 3542 5087 1132 Size: 97 2313 1545 2229 Fragments arranged by size: 2313 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6184 PciI A'CATG_T Cuts at: 5083 5083 Size: 6184 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6184 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4253 4285 5766 5798 4253 Size: 32 1481 32 4639 Fragments arranged by size: 4639 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2794 2794 Size: 6184 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6184 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PstI C_TGCA'G Cuts at: 830 3536 830 Size: 2706 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 PvuII CAG'CTG Cuts at: 2091 2091 Size: 6184 SalI G'TCGA_C Cuts at: 1936 3526 1936 Size: 1590 4594 SbfI CC_TGCA'GG Cuts at: 3536 3536 Size: 6184 ScaI AGT'ACT Cuts at: 4432 6144 4432 Size: 1712 4472 SfcI C'TryA_G Cuts at: 826 1045 3532 4667 5348 5539 826 Size: 219 2487 1135 681 191 1471 Fragments arranged by size: 2487 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6184 SmaI CCC'GGG Cuts at: 1872 2792 3511 1872 Size: 920 719 4545 Fragments arranged by size: 4545 920 719 SmlI C'TyrA_G Cuts at: 820 1017 1943 4288 5189 5451 820 Size: 197 926 2345 901 262 1553 Fragments arranged by size: 2345 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3975 493 Size: 3482 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 3542 1132 Size: 2410 3774 SspI AAT'ATT Cuts at: 5 52 2161 4108 5 Size: 47 2109 1947 2081 Fragments arranged by size: 2109 2081 1947 47 StuI AGG'CCT Cuts at: 1517 3327 1517 Size: 1810 4374 StyI C'CwwG_G Cuts at: 513 1065 2488 3156 3623 513 Size: 552 1423 668 467 3074 Fragments arranged by size: 3074 1423 668 552 467 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4211 4228 4381 4566 5011 325 Size: 1255 2631 17 153 185 445 1498 Fragments arranged by size: 2631 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2853 3270 4430 Size: 274 80 33 51 1443 876 417 1160 Cuts at: 4430 6142 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1160 876 417 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2010 2042 3294 3326 6144 6176 2010 Size: 32 1252 32 2818 32 2018 Fragments arranged by size: 2818 2018 1252 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6184 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6184 XmaI C'CCGG_G Cuts at: 1870 2790 3509 1870 Size: 920 719 4545 Fragments arranged by size: 4545 920 719 XmnI GAAnn'nnTTC Cuts at: 2138 4313 2138 Size: 2175 4009 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3992 276 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI