################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 78 GACGTC GACGT^C AfaI 513 GTAC GT^AC AscI 370 GGCGCGCC GG^CGCGCC Asp700I 492 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 75 GAAGAC GAAGAC(2/6) BglII 476 AGATCT A^GATCT BlpI 30 GCTGAGC GC^T[ACGT]AGC Bpu1102I 30 GCTGAGC GC^T[ACGT]AGC BpuAI 75 GAAGAC GAAGAC(2/6) BsmAI 539 GTCTC GTCTC(1/5) BsmBI 538 CGTCTC CGTCTC(1/5) BsrBI 157 CCGCTC CCGCTC(-3/-3) BstF5I 186 CATCC GGATG(2/0) BstYI 476 AGATCT [GA]^GATC[CT] CelII 30 GCTGAGC GC^T[ACGT]AGC DdeI 31 CTGAG C^T[ACGT]AG EcoNI 423 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 186 CATCC GGATG(9/13) FseI 193 GGCCGGCC GGCCGG^CC HinfI 655 GACTC G^A[ACGT]TC HphI 573 GGTGA GGTGA(8/7) KspI 284 CCGCGG CCGC^GG MaeII 79 ACGT A^CGT MflI 476 AGATCT [GA]^GATC[CT] NotI 286 GCGGCCGC GC^GGCCGC PflMI 344 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 655 GACTC GAGTC(4/5) PshAI 385 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 100 CTGCAG CTGCA^G RsaI 513 GTAC GT^AC SacII 284 CCGCGG CCGC^GG SfaNI 176 GCATC GCATC(5/9) SfcI 100 CTGCAG C^T[GA][CT]AG SgrAI 264 CGCCGGCG C[GA]^CCGG[CT]G SmaI 15 CCCGGG CCC^GGG SmlI 763 CTCAAG C^T[CT][GA]AG TspRI 36 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 344 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 476 AGATCT [GA]^GATC[CT] XmaCI 15 CCCGGG C^CCGGG XmaI 15 CCCGGG C^CCGGG XmnI 492 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### ApaI 280 GGGCCC GGGCC^C 522 GGGCCC BanI 123 GGCGCC G^G[CT][GA]CC 335 GGCGCC BbeI 123 GGCGCC GGCGC^C 335 GGCGCC BpmI 378 CTGGAG CTGGAG(16/14) 487 CTGGAG BsgI 55 CTGCAC GTGCAG(16/14) 364 GTGCAG BstXI 311 CCAAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 509 CCAAGTACCTGG DpnII 477 GATC ^GATC 750 GATC DsaI 200 CCGTGG C^C[GA][CT]GG 284 CCGCGG KasI 123 GGCGCC G^GCGCC 335 GGCGCC MboI 477 GATC ^GATC 750 GATC NarI 123 GGCGCC GG^CGCC 335 GGCGCC NdeII 477 GATC ^GATC 750 GATC NlaIII 63 CATG CATG^ 558 CATG PpuMI 163 GGGACCC [GA]G^G[AT]CC[CT] 383 GGGACCC Sau3AI 477 GATC ^GATC 750 GATC SfoI 123 GGCGCC GGC^GCC 335 GGCGCC TaqI 500 TCGA T^CGA 781 TCGA TthHB8I 500 TCGA T^CGA 781 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AcyI AluI AvaI AvaII BanII BbvI BcnI BglI BmyI BsaHI BsaJI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIV Sau96I ScrFI TseI