(Circular) (Six-base) MAPSORT of: pFN21AB7566.seq Check: 4046 from: 1 to: 5536 pFN21AB7566 5536 bp With 182 enzymes: SgfI * March 28, 2012 12:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2087 3346 278 Size: 53 83 186 936 551 1259 2468 Fragments arranged by size: 2468 1259 936 551 186 83 53 AccI GT'mk_AC Cuts at: 1937 2879 1937 Size: 942 4594 Acc65I G'GTAC_C Cuts at: 2857 2857 Size: 5536 AclI AA'CG_TT Cuts at: 1563 3663 4036 1563 Size: 2100 373 3063 Fragments arranged by size: 3063 2100 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2699 3599 4983 5251 1632 Size: 1067 900 1384 268 1917 Fragments arranged by size: 1917 1384 1067 900 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5339 AflIII A'CryG_T Cuts at: 1203 4435 5412 1203 Size: 3232 977 1327 Fragments arranged by size: 3232 1327 977 AgeI A'CCGG_T Cuts at: 4367 4367 Size: 5536 AhdI GACnn_n'nnGTC Cuts at: 4265 4265 Size: 5536 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5536 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5536 AlwNI CAG_nnn'CTG Cuts at: 3279 4851 3279 Size: 1572 3964 ApaI G_GGCC'C Cuts at: 2289 2531 2289 Size: 242 5294 ApaLI G'TGCA_C Cuts at: 3593 4749 3593 Size: 1156 4380 ApoI r'AATT_y Cuts at: 1493 2845 3071 3223 5332 1493 Size: 1352 226 152 2109 1697 Fragments arranged by size: 2109 1697 1352 226 152 AscI GG'CGCG_CC Cuts at: 2376 2376 Size: 5536 AseI AT'TA_AT Cuts at: 160 1032 4090 160 Size: 872 3058 1606 Fragments arranged by size: 3058 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 AvaI C'yCGr_G Cuts at: 1870 1943 2020 2165 2470 2861 1870 Size: 73 77 145 305 391 4545 Fragments arranged by size: 4545 391 305 145 77 73 BamHI G'GATC_C Cuts at: 2866 3233 2866 Size: 367 5169 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2128 2340 2857 4312 618 Size: 325 217 627 341 212 517 1455 1842 Fragments arranged by size: 1842 1455 627 517 341 325 217 212 BanII G_rGCy'C Cuts at: 1391 2259 2289 2531 2855 1391 Size: 868 30 242 324 4072 Fragments arranged by size: 4072 868 324 242 30 BbeI G_GCGC'C Cuts at: 2132 2344 2132 Size: 212 5324 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2087 928 Size: 971 188 4377 Fragments arranged by size: 4377 971 188 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3727 3761 1903 Size: 34 1790 34 3678 Fragments arranged by size: 3678 1790 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3428 4638 3428 Size: 1210 4326 BclI T'GATC_A Cuts at: 1548 1548 Size: 5536 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2051 2300 2811 Size: 107 122 71 631 605 379 249 511 Cuts at: 2811 4147 136 Size: 1336 1525 Fragments arranged by size: 1525 1336 631 605 511 379 249 122 107 71 BglII A'GATC_T Cuts at: 2481 5531 2481 Size: 3050 2486 BlpI GC'TnA_GC Cuts at: 2036 2953 2036 Size: 917 4619 Bme1580I G_kGCm'C Cuts at: 1790 2047 2289 2531 3597 4753 1790 Size: 257 242 242 1066 1156 2573 Fragments arranged by size: 2573 1156 1066 257 242 242 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5536 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3267 4225 442 Size: 416 53 83 399 24 1850 958 1753 Fragments arranged by size: 1850 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5536 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2404 2513 4196 1239 Size: 1165 109 1683 2579 Fragments arranged by size: 2579 1683 1165 109 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2753 3661 4526 4824 2753 Size: 908 865 298 3465 Fragments arranged by size: 3465 908 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4199 882 Size: 617 2700 2219 Fragments arranged by size: 2700 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3327 493 Size: 711 2123 2702 Fragments arranged by size: 2702 2123 711 BsaBI GATnn'nnATC Cuts at: 5530 5530 Size: 5536 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2084 2129 2341 2424 Size: 53 83 186 936 551 45 212 83 Cuts at: 2424 2639 2667 2829 3343 3725 4360 275 Size: 215 28 162 514 382 635 1451 Fragments arranged by size: 1451 936 635 551 514 382 215 212 186 162 83 83 53 45 28 BsaWI w'CCGG_w Cuts at: 3969 4367 4641 4788 5199 3969 Size: 398 274 147 411 4306 Fragments arranged by size: 4306 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2624 2654 1178 Size: 30 165 30 1221 30 4060 Fragments arranged by size: 4060 1221 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5536 BseYI C'CCAG_C Cuts at: 2137 2557 4739 5235 2137 Size: 420 2182 496 2438 Fragments arranged by size: 2438 2182 496 420 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2045 2390 1163 Size: 573 309 345 4309 Fragments arranged by size: 4309 573 345 309 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2118 2295 2418 2986 3747 4775 Size: 275 186 117 177 123 568 761 1028 Cuts at: 4775 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 568 275 186 177 123 117 BsiHKAI G_wGCw'C Cuts at: 3597 3682 4753 3597 Size: 85 1071 4380 Fragments arranged by size: 4380 1071 85 BsmI GAATG_Cn' Cuts at: 3047 3140 3047 Size: 93 5443 BsmBI CGTCTCn'nnnn_ Cuts at: 2549 2549 Size: 5536 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2047 2259 2289 2531 2855 3597 3682 Size: 399 257 212 30 242 324 742 85 Cuts at: 3682 4753 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 324 257 242 212 85 30 BspEI T'CCGG_A Cuts at: 5199 5199 Size: 5536 BspHI T'CATG_A Cuts at: 3425 5155 3425 Size: 1730 3806 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2891 844 Size: 2047 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2164 3423 1394 Size: 429 341 1259 3507 Fragments arranged by size: 3507 1259 429 341 BsrDI GCAATG_nn' Cuts at: 65 1248 4031 4205 65 Size: 1183 2783 174 1396 Fragments arranged by size: 2783 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2117 2199 2270 2417 2721 4180 4367 Size: 110 307 82 71 147 304 1459 187 Cuts at: 4367 1700 Size: 2869 Fragments arranged by size: 2869 1459 307 304 187 147 110 82 71 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5536 BssHII G'CGCG_C Cuts at: 1925 2151 2303 2376 2536 2538 1925 Size: 226 152 73 160 2 4923 Fragments arranged by size: 4923 226 160 152 73 2 BssSI C'ACGA_G Cuts at: 3596 4608 3596 Size: 1012 4524 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5536 BstBI TT'CG_AA Cuts at: 4340 4340 Size: 5536 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5536 BstXI CCAn_nnnn'nTGG Cuts at: 2323 2521 2323 Size: 198 5338 BstYI r'GATC_y Cuts at: 1972 2481 2866 3233 3618 3635 5087 5173 5185 Size: 509 385 367 385 17 1452 86 12 Cuts at: 5185 5531 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 509 385 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2205 2289 513 Size: 552 939 201 84 3760 Fragments arranged by size: 3760 939 552 201 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4797 Fragments arranged by size: 4797 683 56 BtsI GCAGTG_nn' Cuts at: 2047 3052 3846 3866 2047 Size: 1005 794 20 3717 Fragments arranged by size: 3717 1005 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5536 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5501 DraI TTT'AAA Cuts at: 2840 2840 Size: 5536 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4543 809 Size: 507 3227 1802 Fragments arranged by size: 3227 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2070 2115 Size: 54 1425 50 275 18 222 18 45 Cuts at: 2115 2292 2325 2415 2643 2983 3872 5501 8 Size: 177 33 90 228 340 889 1629 43 Fragments arranged by size: 1629 1425 889 340 275 228 222 177 90 54 50 45 43 33 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2115 2292 2415 2983 1537 Size: 275 303 177 123 568 4090 Fragments arranged by size: 4090 568 303 275 177 123 EarI CTCTTCn'nnn_ Cuts at: 1353 3466 1353 Size: 2113 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2257 2645 4123 4497 4643 387 Size: 1064 138 668 388 1478 374 146 1280 Fragments arranged by size: 1478 1280 1064 668 388 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2404 2513 2699 3599 4196 4983 5251 Size: 393 772 109 186 900 597 787 268 Cuts at: 5251 1239 Size: 1524 Fragments arranged by size: 1524 900 787 772 597 393 268 186 109 EcoNI CCTnn'n_nnAGG Cuts at: 2432 2432 Size: 5536 EcoO109I rG'GnC_Cy Cuts at: 2143 2169 2224 2389 2143 Size: 26 55 165 5290 Fragments arranged by size: 5290 165 55 26 EcoRI G'AATT_C Cuts at: 2845 3223 2845 Size: 378 5158 FseI GG_CCGG'CC Cuts at: 2203 2203 Size: 5536 FspI TGC'GCA Cuts at: 4042 4042 Size: 5536 HaeII r_GCGC'y Cuts at: 1678 2132 2344 2798 4683 1678 Size: 454 212 454 1885 2531 Fragments arranged by size: 2531 1885 454 454 212 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2624 2656 2723 2755 4177 Size: 32 135 32 1052 32 67 32 1422 Cuts at: 4177 4209 4251 4283 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1422 1052 135 67 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2880 3126 669 Size: 988 281 942 246 3079 Fragments arranged by size: 3079 988 942 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5536 HpaI GTT'AAC Cuts at: 3126 3126 Size: 5536 KasI G'GCGC_C Cuts at: 2128 2340 2128 Size: 212 5324 KpnI G_GTAC'C Cuts at: 2861 2861 Size: 5536 MfeI C'AATT_G Cuts at: 3135 3135 Size: 5536 MluI A'CGCG_T Cuts at: 5412 5412 Size: 5536 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4650 4834 1305 Size: 3345 184 2007 Fragments arranged by size: 3345 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3714 Fragments arranged by size: 3714 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3494 3853 4012 518 Size: 648 2328 359 159 2042 Fragments arranged by size: 2328 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2291 2297 2952 3629 4777 5022 1606 Size: 444 241 6 655 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 655 444 245 241 6 NaeI GCC'GGC Cuts at: 2119 2201 2272 2419 2119 Size: 82 71 147 5236 Fragments arranged by size: 5236 147 82 71 NarI GG'CG_CC Cuts at: 2129 2341 2129 Size: 212 5324 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4045 Fragments arranged by size: 4045 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5536 NgoMIV G'CCGG_C Cuts at: 2117 2199 2270 2417 2117 Size: 82 71 147 5236 Fragments arranged by size: 5236 147 82 71 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5536 NotI GC'GGCC_GC Cuts at: 2292 2983 2292 Size: 691 4845 NspI r_CATG'y Cuts at: 1132 1229 2894 4439 1132 Size: 97 1665 1545 2229 Fragments arranged by size: 2229 1665 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5536 PciI A'CATG_T Cuts at: 4435 4435 Size: 5536 PflMI CCAn_nnn'nTGG Cuts at: 1108 2355 1108 Size: 1247 4289 PfoI T'CCnGG_A Cuts at: 2427 2427 Size: 5536 PmeI GTTT'AAAC Cuts at: 2840 2840 Size: 5536 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3605 3637 5118 5150 3605 Size: 32 1481 32 3991 Fragments arranged by size: 3991 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2169 2389 2169 Size: 220 5316 PshAI GACnn'nnGTC Cuts at: 1613 2394 1613 Size: 781 4755 PsiI TTA'TAA Cuts at: 3106 3106 Size: 5536 PspOMI G'GGCC_C Cuts at: 2285 2527 2285 Size: 242 5294 PstI C_TGCA'G Cuts at: 830 2109 2888 830 Size: 1279 779 3478 Fragments arranged by size: 3478 1279 779 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5536 SacII CC_GC'GG Cuts at: 2292 2292 Size: 5536 SalI G'TCGA_C Cuts at: 1936 2878 1936 Size: 942 4594 SanDI GG'GwC_CC Cuts at: 2169 2389 2169 Size: 220 5316 SbfI CC_TGCA'GG Cuts at: 2888 2888 Size: 5536 ScaI AGT'ACT Cuts at: 3784 5496 3784 Size: 1712 3824 SfcI C'TryA_G Cuts at: 826 1045 2105 2884 4019 4700 4891 826 Size: 219 1060 779 1135 681 191 1471 Fragments arranged by size: 1471 1135 1060 779 681 219 191 SfoI GGC'GCC Cuts at: 2130 2342 2130 Size: 212 5324 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 SgrAI Cr'CCGG_yG Cuts at: 1700 2270 1700 Size: 570 4966 SmaI CCC'GGG Cuts at: 1872 2022 2863 1872 Size: 150 841 4545 Fragments arranged by size: 4545 841 150 SmlI C'TyrA_G Cuts at: 820 1017 1943 2768 3640 4541 4803 820 Size: 197 926 825 872 901 262 1553 Fragments arranged by size: 1553 926 901 872 825 262 197 SnaBI TAC'GTA Cuts at: 493 3327 493 Size: 2834 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5536 SphI G_CATG'C Cuts at: 1132 2894 1132 Size: 1762 3774 SrfI GCCC'GGGC Cuts at: 2022 2022 Size: 5536 SspI AAT'ATT Cuts at: 5 52 3460 5 Size: 47 3408 2081 Fragments arranged by size: 3408 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5536 StyI C'CwwG_G Cuts at: 513 1065 2004 2832 2975 513 Size: 552 939 828 143 3074 Fragments arranged by size: 3074 939 828 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2582 3563 3580 3733 3918 4363 325 Size: 1255 1002 981 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1002 981 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3782 5494 96 Size: 274 80 33 51 1443 1805 1712 138 Fragments arranged by size: 1805 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5496 5528 5496 Size: 32 5504 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5536 XbaI T'CTAG_A Cuts at: 2872 2872 Size: 5536 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5536 XmaI C'CCGG_G Cuts at: 1870 2020 2861 1870 Size: 150 841 4545 Fragments arranged by size: 4545 841 150 XmnI GAAnn'nnTTC Cuts at: 2501 3665 2501 Size: 1164 4372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2085 3344 276 Size: 53 83 186 936 551 1259 2468 Fragments arranged by size: 2468 1259 936 551 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SwaI XcmI