(Linear) (Six-base) MAPSORT of: KIEE6455.seq Check: 8204 from: 1 to: 1707 KIEE6455.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AatII G_ACGT'C Cuts at: 0 1685 1707 Size: 1685 22 AccI GT'mk_AC Cuts at: 0 671 1707 Size: 671 1036 AclI AA'CG_TT Cuts at: 0 1221 1707 Size: 1221 486 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 588 1490 1707 Size: 588 902 217 Fragments arranged by size: 902 588 217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 761 1707 Size: 761 946 ApaI G_GGCC'C Cuts at: 0 212 406 1707 Size: 212 194 1301 Fragments arranged by size: 1301 212 194 ApoI r'AATT_y Cuts at: 0 1611 1707 Size: 1611 96 AvaI C'yCGr_G Cuts at: 0 70 217 399 476 658 1707 Size: 70 147 182 77 182 1049 Fragments arranged by size: 1049 182 182 147 77 70 AvrII C'CTAG_G Cuts at: 0 762 1707 Size: 762 945 BamHI G'GATC_C Cuts at: 0 892 1707 Size: 892 815 BanI G'GyrC_C Cuts at: 0 244 441 701 758 1707 Size: 244 197 260 57 949 Fragments arranged by size: 949 260 244 197 57 BanII G_rGCy'C Cuts at: 0 212 406 420 1707 Size: 212 194 14 1287 Fragments arranged by size: 1287 212 194 14 BbeI G_GCGC'C Cuts at: 0 248 445 762 1707 Size: 248 197 317 945 Fragments arranged by size: 945 317 248 197 BbsI GAAGACnn'nnnn_ Cuts at: 0 1380 1707 Size: 1380 327 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 15 49 273 307 1707 Size: 15 34 224 34 1400 Fragments arranged by size: 1400 224 34 34 15 BfrBI ATG'CAT Cuts at: 0 1540 1573 1707 Size: 1540 33 134 Fragments arranged by size: 1540 134 33 BglI GCCn_nnn'nGGC Cuts at: 0 253 661 1707 Size: 253 408 1046 Fragments arranged by size: 1046 408 253 BlpI GC'TnA_GC Cuts at: 0 158 526 1707 Size: 158 368 1181 Fragments arranged by size: 1181 368 158 Bme1580I G_kGCm'C Cuts at: 0 212 406 1707 Size: 212 194 1301 Fragments arranged by size: 1301 212 194 BmrI ACTGGGnnnn_n' Cuts at: 0 206 1707 Size: 206 1501 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 408 1269 1707 Size: 127 281 861 438 Fragments arranged by size: 861 438 281 127 Bpu10I CC'TnA_GC Cuts at: 0 553 1707 Size: 553 1154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 817 1707 Size: 817 890 BsaI GGTCTCn'nnnn_ Cuts at: 0 226 1707 Size: 226 1481 BsaHI Gr'CG_yC Cuts at: 0 245 442 625 759 1682 1707 Size: 245 197 183 134 923 25 Fragments arranged by size: 923 245 197 183 134 25 BseYI C'CCAG_C Cuts at: 0 65 540 681 1707 Size: 65 475 141 1026 Fragments arranged by size: 1026 475 141 65 BsiEI CG_ry'CG Cuts at: 0 343 671 1707 Size: 343 328 1036 Fragments arranged by size: 1036 343 328 BsiHKAI G_wGCw'C Cuts at: 0 794 1707 Size: 794 913 Bsp1286I G_dGCh'C Cuts at: 0 212 406 420 794 1707 Size: 212 194 14 374 913 Fragments arranged by size: 913 374 212 194 14 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 260 1707 Size: 260 1447 BsrBI CCG'CTC Cuts at: 0 98 422 734 1707 Size: 98 324 312 973 Fragments arranged by size: 973 324 312 98 BsrDI GCAATG_nn' Cuts at: 0 137 1575 1707 Size: 137 1438 132 Fragments arranged by size: 1438 137 132 BsrFI r'CCGG_y Cuts at: 0 294 338 698 1707 Size: 294 44 360 1009 Fragments arranged by size: 1009 360 294 44 BstXI CCAn_nnnn'nTGG Cuts at: 0 853 1707 Size: 853 854 BstYI r'GATC_y Cuts at: 0 892 1707 Size: 892 815 BtgI C'CryG_G Cuts at: 0 436 569 665 715 1707 Size: 436 133 96 50 992 Fragments arranged by size: 992 436 133 96 50 BtsI GCAGTG_nn' Cuts at: 0 28 118 1707 Size: 28 90 1589 Fragments arranged by size: 1589 90 28 EaeI y'GGCC_r Cuts at: 0 254 296 317 329 340 572 650 839 Size: 254 42 21 12 11 232 78 189 Cuts at: 839 855 1707 Size: 16 852 Fragments arranged by size: 852 254 232 189 78 42 21 16 12 11 EagI C'GGCC_G Cuts at: 0 340 1707 Size: 340 1367 EarI CTCTTCn'nnn_ Cuts at: 0 85 1707 Size: 85 1622 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 372 509 1707 Size: 372 137 1198 Fragments arranged by size: 1198 372 137 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 408 588 1269 1490 1707 Size: 127 281 180 681 221 217 Fragments arranged by size: 681 281 221 217 180 127 EcoNI CCTnn'n_nnAGG Cuts at: 0 256 832 1707 Size: 256 576 875 Fragments arranged by size: 875 576 256 EcoO109I rG'GnC_Cy Cuts at: 0 208 354 549 645 1131 1275 1707 Size: 208 146 195 96 486 144 432 Fragments arranged by size: 486 432 208 195 146 144 96 EcoRI G'AATT_C Cuts at: 0 1611 1707 Size: 1611 96 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 702 1707 Size: 702 1005 FspI TGC'GCA Cuts at: 0 268 1707 Size: 268 1439 HaeII r_GCGC'y Cuts at: 0 248 432 445 598 762 1707 Size: 248 184 13 153 164 945 Fragments arranged by size: 945 248 184 164 153 13 HincII GTy'rAC Cuts at: 0 672 1428 1707 Size: 672 756 279 Fragments arranged by size: 756 672 279 KasI G'GCGC_C Cuts at: 0 244 441 758 1707 Size: 244 197 317 949 Fragments arranged by size: 949 317 244 197 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 49 920 1707 Size: 49 871 787 Fragments arranged by size: 871 787 49 MscI TGG'CCA Cuts at: 0 857 1707 Size: 857 850 MspA1I CmG'CkG Cuts at: 0 162 279 302 438 447 582 630 667 Size: 162 117 23 136 9 135 48 37 Cuts at: 667 717 1085 1707 Size: 50 368 622 Fragments arranged by size: 622 368 162 136 135 117 50 48 37 23 9 NaeI GCC'GGC Cuts at: 0 296 700 1707 Size: 296 404 1007 Fragments arranged by size: 1007 404 296 NarI GG'CG_CC Cuts at: 0 245 442 759 1707 Size: 245 197 317 948 Fragments arranged by size: 948 317 245 197 NgoMIV G'CCGG_C Cuts at: 0 294 698 1707 Size: 294 404 1009 Fragments arranged by size: 1009 404 294 NsiI A_TGCA'T Cuts at: 0 1542 1575 1707 Size: 1542 33 132 Fragments arranged by size: 1542 132 33 NspI r_CATG'y Cuts at: 0 366 1707 Size: 366 1341 PflMI CCAn_nnn'nTGG Cuts at: 0 864 1707 Size: 864 843 PfoI T'CCnGG_A Cuts at: 0 356 1707 Size: 356 1351 PpuMI rG'GwC_Cy Cuts at: 0 354 645 1131 1707 Size: 354 291 486 576 Fragments arranged by size: 576 486 354 291 PspOMI G'GGCC_C Cuts at: 0 208 402 1707 Size: 208 194 1305 Fragments arranged by size: 1305 208 194 PvuII CAG'CTG Cuts at: 0 1085 1707 Size: 1085 622 SacII CC_GC'GG Cuts at: 0 439 668 718 1707 Size: 439 229 50 989 Fragments arranged by size: 989 439 229 50 SalI G'TCGA_C Cuts at: 0 670 1707 Size: 670 1037 SanDI GG'GwC_CC Cuts at: 0 354 1131 1707 Size: 354 777 576 Fragments arranged by size: 777 576 354 ScaI AGT'ACT Cuts at: 0 1442 1707 Size: 1442 265 SfcI C'TryA_G Cuts at: 0 882 1707 Size: 882 825 SfoI GGC'GCC Cuts at: 0 246 443 760 1707 Size: 246 197 317 947 Fragments arranged by size: 947 317 246 197 SmaI CCC'GGG Cuts at: 0 401 478 660 1707 Size: 401 77 182 1047 Fragments arranged by size: 1047 401 182 77 SmlI C'TyrA_G Cuts at: 0 832 1707 Size: 832 875 StuI AGG'CCT Cuts at: 0 508 1707 Size: 508 1199 StyI C'CwwG_G Cuts at: 0 239 762 1707 Size: 239 523 945 Fragments arranged by size: 945 523 239 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 79 1385 1707 Size: 79 1306 322 Fragments arranged by size: 1306 322 79 TatI w'GTAC_w Cuts at: 0 1440 1707 Size: 1440 267 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 306 1147 1707 Size: 306 841 560 Fragments arranged by size: 841 560 306 XmaI C'CCGG_G Cuts at: 0 399 476 658 1707 Size: 399 77 182 1049 Fragments arranged by size: 1049 399 182 77 XmnI GAAnn'nnTTC Cuts at: 0 1328 1475 1707 Size: 1328 147 232 Fragments arranged by size: 1328 232 147 ZraI GAC'GTC Cuts at: 0 1683 1707 Size: 1683 24 Enzymes that do cut: AatII AccI AclI AcuI AlfI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BfrBI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstXI BstYI BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII HincII KasI MmeI MscI MspA1I NaeI NarI NgoMIV NsiI NspI PflMI PfoI PpuMI PspOMI PvuII SacII SalI SanDI ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaLI AscI AseI AsiSI BaeI BbvCI BciVI BclI BglII BmgBI BmtI BplI BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRV FseI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MslI NcoI NdeI NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI