(Circular) (Six-base) MAPSORT of: pFN21AE0089.seq Check: 6370 from: 1 to: 6829 pFN21AE0089 6829 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4639 278 Size: 53 83 186 936 3103 2468 Fragments arranged by size: 3103 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2745 3295 4172 1937 Size: 808 550 877 4594 Fragments arranged by size: 4594 877 808 550 Acc65I G'GTAC_C Cuts at: 4150 4150 Size: 6829 AclI AA'CG_TT Cuts at: 1563 4956 5329 1563 Size: 3393 373 3063 Fragments arranged by size: 3393 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2589 2697 4892 6276 6544 1632 Size: 957 108 2195 1384 268 1917 Fragments arranged by size: 2195 1917 1384 957 268 108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6632 AflIII A'CryG_T Cuts at: 1203 2277 3281 5728 6705 1203 Size: 1074 1004 2447 977 1327 Fragments arranged by size: 2447 1327 1074 1004 977 AgeI A'CCGG_T Cuts at: 5660 5660 Size: 6829 AhdI GACnn_n'nnGTC Cuts at: 2346 5558 2346 Size: 3212 3617 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6829 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3346 1184 Size: 2162 4667 AlwNI CAG_nnn'CTG Cuts at: 2378 2567 4572 6144 2378 Size: 189 2005 1572 3063 Fragments arranged by size: 3063 2005 1572 189 ApaLI G'TGCA_C Cuts at: 3890 4886 6042 3890 Size: 996 1156 4677 Fragments arranged by size: 4677 1156 996 ApoI r'AATT_y Cuts at: 1493 4138 4364 4516 6625 1493 Size: 2645 226 152 2109 1697 Fragments arranged by size: 2645 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5383 160 Size: 872 4351 1606 Fragments arranged by size: 4351 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6829 AvaI C'yCGr_G Cuts at: 1870 1943 4154 1870 Size: 73 2211 4545 Fragments arranged by size: 4545 2211 73 BamHI G'GATC_C Cuts at: 2164 4159 4526 2164 Size: 1995 367 4467 Fragments arranged by size: 4467 1995 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4150 5605 618 Size: 325 217 627 2363 1455 1842 Fragments arranged by size: 2363 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2608 4148 1391 Size: 1217 1540 4072 Fragments arranged by size: 4072 1540 1217 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3525 928 Size: 971 1626 4232 Fragments arranged by size: 4232 1626 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5020 5054 1903 Size: 34 3083 34 3678 Fragments arranged by size: 3678 3083 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4721 5931 4721 Size: 1210 5619 BclI T'GATC_A Cuts at: 1548 2521 1548 Size: 973 5856 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5440 136 Size: 107 122 71 631 605 3768 1525 Fragments arranged by size: 3768 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6824 6824 Size: 6829 BlpI GC'TnA_GC Cuts at: 4246 4246 Size: 6829 Bme1580I G_kGCm'C Cuts at: 1790 2092 3894 4890 6046 1790 Size: 302 1802 996 1156 2573 Fragments arranged by size: 2573 1802 1156 996 302 BmgBI CAC'GTC Cuts at: 1318 2882 1318 Size: 1564 5265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 2500 3532 Size: 416 53 83 399 24 897 186 1032 Cuts at: 3532 4560 5518 442 Size: 1028 958 1753 Fragments arranged by size: 1753 1032 1028 958 897 416 399 186 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2990 1056 Size: 1934 4895 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2670 2670 Size: 6829 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3576 5489 1239 Size: 2337 1913 2579 Fragments arranged by size: 2579 2337 1913 Bpu10I CC'TnA_GC Cuts at: 3906 3944 3906 Size: 38 6791 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2368 3517 4954 5819 6117 2368 Size: 1149 1437 865 298 3080 Fragments arranged by size: 3080 1437 1149 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5492 882 Size: 617 3993 2219 Fragments arranged by size: 3993 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2046 4620 493 Size: 711 842 2574 2702 Fragments arranged by size: 2702 2574 842 711 BsaBI GATnn'nnATC Cuts at: 2163 6823 2163 Size: 4660 2169 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4636 5018 5653 275 Size: 53 83 186 936 3103 382 635 1451 Fragments arranged by size: 3103 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3069 3315 5262 5660 5934 6081 6492 3069 Size: 246 1947 398 274 147 411 3406 Fragments arranged by size: 3406 1947 411 398 274 246 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2344 2374 2605 2635 3972 Size: 30 165 30 941 30 231 30 1337 Cuts at: 3972 4002 1178 Size: 30 4005 Fragments arranged by size: 4005 1337 941 231 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2352 2883 1186 Size: 1166 531 5132 Fragments arranged by size: 5132 1166 531 BseYI C'CCAG_C Cuts at: 6032 6528 6032 Size: 496 6333 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6256 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4079 4279 5040 6068 1540 Size: 275 186 2078 200 761 1028 2301 Fragments arranged by size: 2301 2078 1028 761 275 200 186 BsiHKAI G_wGCw'C Cuts at: 2608 3894 4890 4975 6046 2608 Size: 1286 996 85 1071 3391 Fragments arranged by size: 3391 1286 1071 996 85 BsmI GAATG_Cn' Cuts at: 4340 4433 4340 Size: 93 6736 BsmBI CGTCTCn'nnnn_ Cuts at: 3213 3213 Size: 6829 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2092 2608 3894 4148 4890 4975 6046 Size: 399 302 516 1286 254 742 85 1071 Cuts at: 6046 1391 Size: 2174 Fragments arranged by size: 2174 1286 1071 742 516 399 302 254 85 BspEI T'CCGG_A Cuts at: 3069 6492 3069 Size: 3423 3406 BspHI T'CATG_A Cuts at: 4718 6448 4718 Size: 1730 5099 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4184 844 Size: 3340 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4716 1394 Size: 429 2893 3507 Fragments arranged by size: 3507 2893 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2874 3045 3149 5324 5498 65 Size: 1183 1626 171 104 2175 174 1396 Fragments arranged by size: 2175 1626 1396 1183 174 171 104 BsrFI r'CCGG_y Cuts at: 1700 1810 5473 5660 1700 Size: 110 3663 187 2869 Fragments arranged by size: 3663 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2282 2950 4082 96 Size: 2186 668 1132 2843 Fragments arranged by size: 2843 2186 1132 668 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6829 BssSI C'ACGA_G Cuts at: 4889 5901 4889 Size: 1012 5817 BstAPI GCAn_nnn'nTGC Cuts at: 794 2983 794 Size: 2189 4640 BstBI TT'CG_AA Cuts at: 5633 5633 Size: 6829 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6829 BstYI r'GATC_y Cuts at: 1972 2122 2164 2375 4071 4159 4526 4911 4928 Size: 150 42 211 1696 88 367 385 17 Cuts at: 4928 6380 6466 6478 6824 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1696 1452 385 367 346 211 150 88 86 42 17 12 Bsu36I CC'TnA_GG Cuts at: 2371 2371 Size: 6829 BtgI C'CryG_G Cuts at: 513 1065 3777 513 Size: 552 2712 3565 Fragments arranged by size: 3565 2712 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6090 Fragments arranged by size: 6090 683 56 BtsI GCAGTG_nn' Cuts at: 4345 5139 5159 4345 Size: 794 20 6015 Fragments arranged by size: 6015 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6829 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6794 DraI TTT'AAA Cuts at: 2131 2752 4133 2131 Size: 621 1381 4827 Fragments arranged by size: 4827 1381 621 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5836 809 Size: 507 4520 1802 Fragments arranged by size: 4520 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2057 2297 4076 Size: 54 1425 50 275 18 227 240 1779 Cuts at: 4076 4276 5165 6794 8 Size: 200 889 1629 43 Fragments arranged by size: 1779 1629 1425 889 275 240 227 200 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4076 4276 1537 Size: 275 2264 200 4090 Fragments arranged by size: 4090 2264 275 200 EarI CTCTTCn'nnn_ Cuts at: 1353 2565 2804 3096 3995 4759 1353 Size: 1212 239 292 899 764 3423 Fragments arranged by size: 3423 1212 899 764 292 239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5416 5790 5936 387 Size: 1064 138 3827 374 146 1280 Fragments arranged by size: 3827 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2606 2606 Size: 6829 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2589 2697 3576 4892 5489 6276 6544 Size: 393 957 108 879 1316 597 787 268 Cuts at: 6544 1239 Size: 1524 Fragments arranged by size: 1524 1316 957 879 787 597 393 268 108 EcoRI G'AATT_C Cuts at: 4138 4516 4138 Size: 378 6451 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2211 2211 Size: 6829 FspI TGC'GCA Cuts at: 5335 5335 Size: 6829 HaeII r_GCGC'y Cuts at: 1678 5976 1678 Size: 4298 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2332 2364 3365 3397 3443 Size: 32 135 32 760 32 1001 32 46 Cuts at: 3443 3475 5470 5502 5544 5576 1373 Size: 32 1995 32 42 32 2626 Fragments arranged by size: 2626 1995 1001 760 135 46 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3296 3865 4173 4419 669 Size: 988 281 1358 569 308 246 3079 Fragments arranged by size: 3079 1358 988 569 308 281 246 HindIII A'AGCT_T Cuts at: 748 3562 748 Size: 2814 4015 HpaI GTT'AAC Cuts at: 4419 4419 Size: 6829 KpnI G_GTAC'C Cuts at: 4154 4154 Size: 6829 MfeI C'AATT_G Cuts at: 4428 4428 Size: 6829 MluI A'CGCG_T Cuts at: 6705 6705 Size: 6829 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3701 5943 6127 1305 Size: 2396 2242 184 2007 Fragments arranged by size: 2396 2242 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2059 2299 10 Size: 54 1425 343 227 240 4540 Fragments arranged by size: 4540 1425 343 240 227 54 MslI CAynn'nnrTG Cuts at: 518 1166 4037 4787 5146 5305 518 Size: 648 2871 750 359 159 2042 Fragments arranged by size: 2871 2042 750 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4245 4922 6070 6315 1606 Size: 2639 677 1148 245 2120 Fragments arranged by size: 2639 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3777 513 Size: 552 2712 3565 Fragments arranged by size: 3565 2712 552 NdeI CA'TA_TG Cuts at: 387 2482 387 Size: 2095 4734 NheI G'CTAG_C Cuts at: 1052 2986 1052 Size: 1934 4895 NotI GC'GGCC_GC Cuts at: 4276 4276 Size: 6829 NspI r_CATG'y Cuts at: 1132 1229 2281 4187 5732 1132 Size: 97 1052 1906 1545 2229 Fragments arranged by size: 2229 1906 1545 1052 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6829 PciI A'CATG_T Cuts at: 2277 5728 2277 Size: 3451 3378 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6829 PmeI GTTT'AAAC Cuts at: 4133 4133 Size: 6829 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4898 4930 6411 6443 4898 Size: 32 1481 32 5284 Fragments arranged by size: 5284 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6829 PsiI TTA'TAA Cuts at: 4399 4399 Size: 6829 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2098 2130 2098 Size: 32 6797 PstI C_TGCA'G Cuts at: 830 3325 4181 830 Size: 2495 856 3478 Fragments arranged by size: 3478 2495 856 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6829 SacI G_AGCT'C Cuts at: 2608 2608 Size: 6829 SalI G'TCGA_C Cuts at: 1936 3294 4171 1936 Size: 1358 877 4594 Fragments arranged by size: 4594 1358 877 SapI GCTCTTCn'nnn_ Cuts at: 2804 2804 Size: 6829 SbfI CC_TGCA'GG Cuts at: 4181 4181 Size: 6829 ScaI AGT'ACT Cuts at: 5077 6789 5077 Size: 1712 5117 SfcI C'TryA_G Cuts at: 826 1045 2352 3321 4177 5312 5993 6184 826 Size: 219 1307 969 856 1135 681 191 1471 Fragments arranged by size: 1471 1307 1135 969 856 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6829 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6829 SmaI CCC'GGG Cuts at: 1872 4156 1872 Size: 2284 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2383 3496 4933 5834 6096 820 Size: 197 926 440 1113 1437 901 262 1553 Fragments arranged by size: 1553 1437 1113 926 901 440 262 197 SnaBI TAC'GTA Cuts at: 493 2046 4620 493 Size: 1553 2574 2702 Fragments arranged by size: 2702 2574 1553 SpeI A'CTAG_T Cuts at: 152 152 Size: 6829 SphI G_CATG'C Cuts at: 1132 4187 1132 Size: 3055 3774 SspI AAT'ATT Cuts at: 5 52 2039 2994 3635 4753 5 Size: 47 1987 955 641 1118 2081 Fragments arranged by size: 2081 1987 1118 955 641 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6829 StyI C'CwwG_G Cuts at: 513 1065 2225 2496 3267 3777 4268 513 Size: 552 1160 271 771 510 491 3074 Fragments arranged by size: 3074 1160 771 552 510 491 271 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4856 4873 5026 5211 5656 325 Size: 1255 3276 17 153 185 445 1498 Fragments arranged by size: 3276 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2075 2282 2950 Size: 274 80 33 51 1443 98 207 668 Cuts at: 2950 3586 4082 5075 6787 96 Size: 636 496 993 1712 138 Fragments arranged by size: 1712 1443 993 668 636 496 274 207 138 98 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6789 6821 6789 Size: 32 6797 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6829 XbaI T'CTAG_A Cuts at: 4165 4165 Size: 6829 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2099 3258 2099 Size: 1159 5670 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6829 XmaI C'CCGG_G Cuts at: 1870 4154 1870 Size: 2284 4545 XmnI GAAnn'nnTTC Cuts at: 4958 4958 Size: 6829 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4637 276 Size: 53 83 186 936 3103 2468 Fragments arranged by size: 3103 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI