(Circular) (Six-base) MAPSORT of: pFN21AE2539.seq Check: 8951 from: 1 to: 6745 pFN21AE2539 6745 bp With 182 enzymes: SgfI * September 19, 2014 18:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4555 278 Size: 53 83 186 936 3019 2468 Fragments arranged by size: 3019 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3777 4088 1937 Size: 1840 311 4594 Fragments arranged by size: 4594 1840 311 Acc65I G'GTAC_C Cuts at: 4066 4066 Size: 6745 AclI AA'CG_TT Cuts at: 1563 3986 4872 5245 1563 Size: 2423 886 373 3063 Fragments arranged by size: 3063 2423 886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2062 2950 3908 4808 6192 6460 1632 Size: 430 888 958 900 1384 268 1917 Fragments arranged by size: 1917 1384 958 900 888 430 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6548 AflIII A'CryG_T Cuts at: 1203 2463 5644 6621 1203 Size: 1260 3181 977 1327 Fragments arranged by size: 3181 1327 1260 977 AgeI A'CCGG_T Cuts at: 5576 5576 Size: 6745 AhdI GACnn_n'nnGTC Cuts at: 3550 5474 3550 Size: 1924 4821 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6745 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6745 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2677 2709 2677 Size: 32 6713 AlwNI CAG_nnn'CTG Cuts at: 4488 6060 4488 Size: 1572 5173 ApaI G_GGCC'C Cuts at: 2646 2646 Size: 6745 ApaLI G'TGCA_C Cuts at: 2984 4802 5958 2984 Size: 1818 1156 3771 Fragments arranged by size: 3771 1818 1156 ApoI r'AATT_y Cuts at: 1493 4017 4054 4280 4432 6541 1493 Size: 2524 37 226 152 2109 1697 Fragments arranged by size: 2524 2109 1697 226 152 37 AseI AT'TA_AT Cuts at: 160 1032 5299 160 Size: 872 4267 1606 Fragments arranged by size: 4267 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6745 AvaI C'yCGr_G Cuts at: 1870 1943 2680 3485 4070 1870 Size: 73 737 805 585 4545 Fragments arranged by size: 4545 805 737 585 73 BamHI G'GATC_C Cuts at: 4075 4442 4075 Size: 367 6378 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4066 5521 618 Size: 325 217 627 2279 1455 1842 Fragments arranged by size: 2279 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2646 3475 3845 4064 1391 Size: 1255 829 370 219 4072 Fragments arranged by size: 4072 1255 829 370 219 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2887 4011 928 Size: 971 988 1124 3662 Fragments arranged by size: 3662 1124 988 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2324 2358 2720 2754 4936 4970 1903 Size: 34 387 34 362 34 2182 34 3678 Fragments arranged by size: 3678 2182 387 362 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2086 3014 4637 5847 2086 Size: 928 1623 1210 2984 Fragments arranged by size: 2984 1623 1210 928 BclI T'GATC_A Cuts at: 1548 1548 Size: 6745 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2650 5356 136 Size: 107 122 71 631 605 978 2706 1525 Fragments arranged by size: 2706 1525 978 631 605 122 107 71 BglII A'GATC_T Cuts at: 3825 6740 3825 Size: 2915 3830 BlpI GC'TnA_GC Cuts at: 4162 4162 Size: 6745 Bme1580I G_kGCm'C Cuts at: 1790 2646 2988 3972 4806 5962 1790 Size: 856 342 984 834 1156 2573 Fragments arranged by size: 2573 1156 984 856 834 342 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6745 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3197 4476 5434 Size: 416 53 83 399 24 1780 1279 958 Cuts at: 5434 442 Size: 1753 Fragments arranged by size: 1780 1753 1279 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6745 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3061 3457 3482 3061 Size: 396 25 6324 Fragments arranged by size: 6324 396 25 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2398 2888 3738 5405 1239 Size: 1159 490 850 1667 2579 Fragments arranged by size: 2579 1667 1159 850 490 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2648 2657 4870 5735 6033 2648 Size: 9 2213 865 298 3360 Fragments arranged by size: 3360 2213 865 298 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2767 3133 3973 5408 882 Size: 617 1268 366 840 1435 2219 Fragments arranged by size: 2219 1435 1268 840 617 366 BsaAI yAC'GTr Cuts at: 493 1204 2466 2617 4536 493 Size: 711 1262 151 1919 2702 Fragments arranged by size: 2702 1919 1262 711 151 BsaBI GATnn'nnATC Cuts at: 6739 6739 Size: 6745 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4552 4934 5569 275 Size: 53 83 186 936 3019 382 635 1451 Fragments arranged by size: 3019 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2126 5178 5576 5850 5997 6408 2126 Size: 3052 398 274 147 411 2463 Fragments arranged by size: 3052 2463 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2541 2571 1178 Size: 30 165 30 1138 30 5352 Fragments arranged by size: 5352 1138 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2565 3037 3539 1186 Size: 1379 472 502 4392 Fragments arranged by size: 4392 1379 502 472 BseYI C'CCAG_C Cuts at: 2013 2275 2589 2741 5948 6444 2013 Size: 262 314 152 3207 496 2314 Fragments arranged by size: 3207 2314 496 314 262 152 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3385 3907 1163 Size: 573 1649 522 4001 Fragments arranged by size: 4001 1649 573 522 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4195 4956 5984 1540 Size: 275 186 2194 761 1028 2301 Fragments arranged by size: 2301 2194 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2197 2988 4806 4891 5962 2197 Size: 791 1818 85 1071 2980 Fragments arranged by size: 2980 1818 1071 791 85 BsmI GAATG_Cn' Cuts at: 4256 4349 4256 Size: 93 6652 BsmBI CGTCTCn'nnnn_ Cuts at: 2547 2547 Size: 6745 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2197 2646 2988 3475 3845 3972 4064 Size: 399 407 449 342 487 370 127 92 Cuts at: 4064 4806 4891 5962 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 487 449 407 399 370 342 127 92 85 BspEI T'CCGG_A Cuts at: 2126 6408 2126 Size: 4282 2463 BspHI T'CATG_A Cuts at: 4634 6364 4634 Size: 1730 5015 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4100 844 Size: 3256 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4632 1394 Size: 429 2809 3507 Fragments arranged by size: 3507 2809 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5240 5414 65 Size: 1183 3992 174 1396 Fragments arranged by size: 3992 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2499 2524 2977 5389 5576 1700 Size: 110 689 25 453 2412 187 2869 Fragments arranged by size: 2869 2412 689 453 187 110 25 BsrGI T'GTAC_A Cuts at: 96 3936 96 Size: 3840 2905 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6745 BssSI C'ACGA_G Cuts at: 4805 5817 4805 Size: 1012 5733 BstAPI GCAn_nnn'nTGC Cuts at: 794 3967 794 Size: 3173 3572 BstBI TT'CG_AA Cuts at: 5549 5549 Size: 6745 BstEII G'GTnAC_C Cuts at: 1184 2041 3274 1184 Size: 857 1233 4655 Fragments arranged by size: 4655 1233 857 BstXI CCAn_nnnn'nTGG Cuts at: 3580 3744 3580 Size: 164 6581 BstYI r'GATC_y Cuts at: 1972 3825 4075 4442 4827 4844 6296 6382 6394 Size: 1853 250 367 385 17 1452 86 12 Cuts at: 6394 6740 1972 Size: 346 1977 Fragments arranged by size: 1977 1853 1452 385 367 346 250 86 17 12 BstZ17I GTA'TAC Cuts at: 3778 3778 Size: 6745 Bsu36I CC'TnA_GG Cuts at: 3522 3522 Size: 6745 BtgI C'CryG_G Cuts at: 513 1065 2004 2046 3279 3864 513 Size: 552 939 42 1233 585 3394 Fragments arranged by size: 3394 1233 939 585 552 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2327 487 Size: 683 56 1101 4905 Fragments arranged by size: 4905 1101 683 56 BtsI GCAGTG_nn' Cuts at: 3246 3972 4261 5055 5075 3246 Size: 726 289 794 20 4916 Fragments arranged by size: 4916 794 726 289 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6745 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6710 DraI TTT'AAA Cuts at: 4049 4049 Size: 6745 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5752 809 Size: 507 4436 1802 Fragments arranged by size: 4436 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 3282 3570 Size: 54 1425 50 275 18 420 1032 288 Cuts at: 3570 4192 5081 6710 8 Size: 622 889 1629 43 Fragments arranged by size: 1629 1425 1032 889 622 420 288 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4192 1537 Size: 275 2380 4090 Fragments arranged by size: 4090 2380 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2827 3100 4675 1353 Size: 1474 273 1575 3423 Fragments arranged by size: 3423 1575 1474 273 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2750 3248 5332 5706 5852 387 Size: 1064 138 1161 498 2084 374 146 1280 Fragments arranged by size: 2084 1280 1161 1064 498 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2062 2398 2888 2950 3738 3908 4808 Size: 393 430 336 490 62 788 170 900 Cuts at: 4808 5405 6192 6460 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 900 788 787 597 490 430 393 336 268 170 62 EcoO109I rG'GnC_Cy Cuts at: 3151 3151 Size: 6745 EcoRI G'AATT_C Cuts at: 4017 4054 4432 4017 Size: 37 378 6330 Fragments arranged by size: 6330 378 37 EcoRV GAT'ATC Cuts at: 3691 3691 Size: 6745 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2980 2980 Size: 6745 FspI TGC'GCA Cuts at: 5251 5251 Size: 6745 HaeII r_GCGC'y Cuts at: 1678 2828 5892 1678 Size: 1150 3064 2531 Fragments arranged by size: 3064 2531 1150 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2105 2137 3320 3352 3536 Size: 32 135 32 533 32 1183 32 184 Cuts at: 3536 3568 3785 3817 5386 5418 5460 5492 1373 Size: 32 217 32 1569 32 42 32 2626 Fragments arranged by size: 2626 1569 1183 533 217 184 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4089 4335 669 Size: 988 281 2151 246 3079 Fragments arranged by size: 3079 2151 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6745 HpaI GTT'AAC Cuts at: 4335 4335 Size: 6745 KpnI G_GTAC'C Cuts at: 4070 4070 Size: 6745 MfeI C'AATT_G Cuts at: 4344 4344 Size: 6745 MluI A'CGCG_T Cuts at: 6621 6621 Size: 6745 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2527 3084 5859 6043 1305 Size: 1222 557 2775 184 2007 Fragments arranged by size: 2775 2007 1222 557 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3572 10 Size: 54 1425 343 1740 3183 Fragments arranged by size: 3183 1740 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2475 3113 3789 3944 4703 5062 5221 Size: 648 1309 638 676 155 759 359 159 Cuts at: 5221 518 Size: 2042 Fragments arranged by size: 2042 1309 759 676 648 638 359 159 155 MspA1I CmG'CkG Cuts at: 1606 2013 3070 3568 4161 4838 5986 6231 1606 Size: 407 1057 498 593 677 1148 245 2120 Fragments arranged by size: 2120 1148 1057 677 593 498 407 245 NaeI GCC'GGC Cuts at: 2526 2979 2526 Size: 453 6292 NcoI C'CATG_G Cuts at: 513 1065 2004 2046 513 Size: 552 939 42 5212 Fragments arranged by size: 5212 939 552 42 NdeI CA'TA_TG Cuts at: 387 387 Size: 6745 NgoMIV G'CCGG_C Cuts at: 2524 2977 2524 Size: 453 6292 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6745 NotI GC'GGCC_GC Cuts at: 4192 4192 Size: 6745 NspI r_CATG'y Cuts at: 1132 1229 3965 4103 5648 1132 Size: 97 2736 138 1545 2229 Fragments arranged by size: 2736 2229 1545 138 97 PasI CC'CwG_GG Cuts at: 1348 3199 3854 1348 Size: 1851 655 4239 Fragments arranged by size: 4239 1851 655 PciI A'CATG_T Cuts at: 5644 5644 Size: 6745 PflMI CCAn_nnn'nTGG Cuts at: 1108 2307 1108 Size: 1199 5546 PmeI GTTT'AAAC Cuts at: 4049 4049 Size: 6745 PmlI CAC'GTG Cuts at: 2617 2617 Size: 6745 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4814 4846 6327 6359 4814 Size: 32 1481 32 5200 Fragments arranged by size: 5200 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3151 3151 Size: 6745 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6745 PsiI TTA'TAA Cuts at: 4315 4315 Size: 6745 PspOMI G'GGCC_C Cuts at: 2642 2642 Size: 6745 PstI C_TGCA'G Cuts at: 830 4097 830 Size: 3267 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6745 PvuII CAG'CTG Cuts at: 2013 3568 2013 Size: 1555 5190 SalI G'TCGA_C Cuts at: 1936 4087 1936 Size: 2151 4594 SbfI CC_TGCA'GG Cuts at: 4097 4097 Size: 6745 ScaI AGT'ACT Cuts at: 3497 3587 4993 6705 3497 Size: 90 1406 1712 3537 Fragments arranged by size: 3537 1712 1406 90 SfcI C'TryA_G Cuts at: 826 1045 4093 5228 5909 6100 826 Size: 219 3048 1135 681 191 1471 Fragments arranged by size: 3048 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6745 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6745 SmaI CCC'GGG Cuts at: 1872 3487 4072 1872 Size: 1615 585 4545 Fragments arranged by size: 4545 1615 585 SmlI C'TyrA_G Cuts at: 820 1017 1943 2663 2672 4849 5750 6012 820 Size: 197 926 720 9 2177 901 262 1553 Fragments arranged by size: 2177 1553 926 901 720 262 197 9 SnaBI TAC'GTA Cuts at: 493 4536 493 Size: 4043 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6745 SphI G_CATG'C Cuts at: 1132 3965 4103 1132 Size: 2833 138 3774 Fragments arranged by size: 3774 2833 138 SspI AAT'ATT Cuts at: 5 52 4669 5 Size: 47 4617 2081 Fragments arranged by size: 4617 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6745 StyI C'CwwG_G Cuts at: 513 1065 2004 2046 3264 4184 513 Size: 552 939 42 1218 920 3074 Fragments arranged by size: 3074 1218 939 920 552 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2744 4772 4789 4942 5127 5572 325 Size: 1255 1164 2028 17 153 185 445 1498 Fragments arranged by size: 2028 1498 1255 1164 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3495 3585 3936 Size: 274 80 33 51 1443 1518 90 351 Cuts at: 3936 4991 6703 96 Size: 1055 1712 138 Fragments arranged by size: 1712 1518 1443 1055 351 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6705 6737 6705 Size: 32 6713 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6745 XbaI T'CTAG_A Cuts at: 4081 4081 Size: 6745 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2304 3067 3634 2304 Size: 763 567 5415 Fragments arranged by size: 5415 763 567 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6745 XmaI C'CCGG_G Cuts at: 1870 3485 4070 1870 Size: 1615 585 4545 Fragments arranged by size: 4545 1615 585 XmnI GAAnn'nnTTC Cuts at: 4874 4874 Size: 6745 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4553 276 Size: 53 83 186 936 3019 2468 Fragments arranged by size: 3019 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI DraIII EcoICRI EcoNI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI