################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraIII DrdI Eam1105I EarI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HinfI HpaI KpnI Ksp632I MaeI MaeIII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NdeI NdeII NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI Sau3AI SbfI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AfaI 789 GTAC GT^AC AlwNI 120 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 437 AGCGCT AGC^GCT AvaII 350 GGTCC G^G[AT]CC BbrPI 741 CACGTG CAC^GTG BlpI 450 GCTGAGC GC^T[ACGT]AGC Bpu1102I 450 GCTGAGC GC^T[ACGT]AGC BsgI 583 CTGCAC GTGCAG(16/14) BspMI 633 GCAGGT ACCTGC(4/8) BsrFI 291 GCCGGC [GA]^CCGG[CT] BssSI 64 CTCGTG CACGAG(-5/-1) BstXI 161 CCAAAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 450 GCTGAGC GC^T[ACGT]AGC Cfr10I 291 GCCGGC [GA]^CCGG[CT] DdeI 451 CTGAG C^T[ACGT]AG Eco47III 437 AGCGCT AGC^GCT EcoT14I 56 CCATGG C^C[AT][AT]GG HgaI 537 GACGC GACGC(5/10) HphI 158 TCACC GGTGA(8/7) NaeI 291 GCCGGC GCC^GGC NcoI 56 CCATGG C^CATGG NgoMIV 291 GCCGGC G^CCGGC NlaIII 57 CATG CATG^ PmaCI 741 CACGTG CAC^GTG PmlI 741 CACGTG CAC^GTG PpuMI 349 GGGTCCC [GA]G^G[AT]CC[CT] PstI 631 CTGCAG CTGCA^G RsaI 789 GTAC GT^AC ScaI 788 AGTACT AGT^ACT SfcI 631 CTGCAG C^T[GA][CT]AG SmlI 694 CTCAAG C^T[CT][GA]AG StyI 56 CCATGG C^C[AT][AT]GG Tsp509I 383 AATT ^AATT ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AflIII 740 ACACGT A^C[GA][CT]GT 742 ACGTGT BanI 18 GGCGCC G^G[CT][GA]CC 757 GGCGCC BbeI 18 GGCGCC GGCGC^C 757 GGCGCC BglI 592 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 854 GCCCGCCTGGC BpmI 394 CTGGAG CTGGAG(16/14) 430 CTGGAG BsaAI 82 TACGTG [CT]AC^GT[GA] 741 CACGTG KasI 18 GGCGCC G^GCGCC 757 GGCGCC KspI 36 CCGCGG CCGC^GG 198 CCGCGG MaeII 83 ACGT A^CGT 742 ACGT NarI 18 GGCGCC GG^CGCC 757 GGCGCC NotI 38 GCGGCCGC GC^GGCCGC 604 GCGGCCGC SacII 36 CCGCGG CCGC^GG 198 CCGCGG SfoI 18 GGCGCC GGC^GCC 757 GGCGCC SmaI 93 CCCGGG CCC^GGG 353 CCCGGG XmaCI 93 CCCGGG C^CCGGG 353 CCCGGG XmaI 93 CCCGGG C^CCGGG 353 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AvaI BanII BbvI BcnI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI