(Circular) (Six-base) MAPSORT of: pFN21AE0290.seq Check: 5309 from: 1 to: 5581 pFN21AE0290 5581 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2629 2629 Size: 5581 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3391 278 Size: 53 83 186 936 1855 2468 Fragments arranged by size: 2468 1855 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2924 1937 Size: 987 4594 Acc65I G'GTAC_C Cuts at: 2902 2902 Size: 5581 AclI AA'CG_TT Cuts at: 1563 3708 4081 1563 Size: 2145 373 3063 Fragments arranged by size: 3063 2145 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2107 2645 3644 5028 5296 1632 Size: 475 538 999 1384 268 1917 Fragments arranged by size: 1917 1384 999 538 475 268 AfeI AGC'GCT Cuts at: 2444 2444 Size: 5581 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5384 AflIII A'CryG_T Cuts at: 1203 2745 2747 4480 5457 1203 Size: 1542 2 1733 977 1327 Fragments arranged by size: 1733 1542 1327 977 2 AgeI A'CCGG_T Cuts at: 4412 4412 Size: 5581 AhdI GACnn_n'nnGTC Cuts at: 4310 4310 Size: 5581 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5581 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2891 1184 Size: 1707 3874 AlwNI CAG_nnn'CTG Cuts at: 2130 3324 4896 2130 Size: 1194 1572 2815 Fragments arranged by size: 2815 1572 1194 ApaI G_GGCC'C Cuts at: 2105 2417 2563 2708 2105 Size: 312 146 145 4978 Fragments arranged by size: 4978 312 146 145 ApaLI G'TGCA_C Cuts at: 3638 4794 3638 Size: 1156 4425 ApoI r'AATT_y Cuts at: 1493 2890 3116 3268 5377 1493 Size: 1397 226 152 2109 1697 Fragments arranged by size: 2109 1697 1397 226 152 AseI AT'TA_AT Cuts at: 160 1032 4135 160 Size: 872 3103 1606 Fragments arranged by size: 3103 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5581 AvaI C'yCGr_G Cuts at: 1870 1943 2098 2358 2630 2906 1870 Size: 73 155 260 272 276 4545 Fragments arranged by size: 4545 276 272 260 155 73 BamHI G'GATC_C Cuts at: 2911 3278 2911 Size: 367 5214 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2023 2762 2902 4357 618 Size: 325 217 627 236 739 140 1455 1842 Fragments arranged by size: 1842 1455 739 627 325 236 217 140 BanII G_rGCy'C Cuts at: 1391 2105 2151 2417 2462 2563 2583 2708 2900 Size: 714 46 266 45 101 20 125 192 Cuts at: 2900 1391 Size: 4072 Fragments arranged by size: 4072 714 266 192 125 101 46 45 20 BbeI G_GCGC'C Cuts at: 2027 2766 2027 Size: 739 4842 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4610 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3772 3806 1903 Size: 34 1835 34 3678 Fragments arranged by size: 3678 1835 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3473 4683 3473 Size: 1210 4371 BclI T'GATC_A Cuts at: 1548 1548 Size: 5581 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2603 2865 Size: 107 122 71 631 605 337 594 262 Cuts at: 2865 4192 136 Size: 1327 1525 Fragments arranged by size: 1525 1327 631 605 594 337 262 122 107 71 BglII A'GATC_T Cuts at: 5576 5576 Size: 5581 BlpI GC'TnA_GC Cuts at: 2456 2998 2456 Size: 542 5039 Bme1580I G_kGCm'C Cuts at: 1790 2076 2105 2417 2563 2708 3642 4798 1790 Size: 286 29 312 146 145 934 1156 2573 Fragments arranged by size: 2573 1156 934 312 286 146 145 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5581 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3312 4270 442 Size: 416 53 83 399 24 1895 958 1753 Fragments arranged by size: 1895 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5581 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2420 2456 4241 1239 Size: 1181 36 1785 2579 Fragments arranged by size: 2579 1785 1181 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2684 3706 4571 4869 2684 Size: 1022 865 298 3396 Fragments arranged by size: 3396 1022 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4244 882 Size: 617 2745 2219 Fragments arranged by size: 2745 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2089 2748 3372 493 Size: 711 885 659 624 2702 Fragments arranged by size: 2702 885 711 659 624 BsaBI GATnn'nnATC Cuts at: 5575 5575 Size: 5581 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2024 2543 2763 3388 Size: 53 83 186 936 491 519 220 625 Cuts at: 3388 3770 4405 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 625 519 491 382 220 186 83 53 BsaWI w'CCGG_w Cuts at: 4014 4412 4686 4833 5244 4014 Size: 398 274 147 411 4351 Fragments arranged by size: 4351 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2582 2612 1178 Size: 30 165 30 1179 30 4147 Fragments arranged by size: 4147 1179 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2227 2230 2233 2453 2567 1186 Size: 1041 3 3 220 114 4200 Fragments arranged by size: 4200 1041 220 114 3 3 BseYI C'CCAG_C Cuts at: 2075 2290 2302 2491 4784 5280 2075 Size: 215 12 189 2293 496 2376 Fragments arranged by size: 2376 2293 496 215 189 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2573 1163 Size: 573 837 4171 Fragments arranged by size: 4171 837 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2047 2577 2613 3031 3792 4820 Size: 275 186 46 530 36 418 761 1028 Cuts at: 4820 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 530 418 275 186 46 36 BsiHKAI G_wGCw'C Cuts at: 3642 3727 4798 3642 Size: 85 1071 4425 Fragments arranged by size: 4425 1071 85 BsmI GAATG_Cn' Cuts at: 3092 3185 3092 Size: 93 5488 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2105 2151 2417 2462 2563 2583 Size: 399 286 29 46 266 45 101 20 Cuts at: 2583 2708 2900 3642 3727 4798 1391 Size: 125 192 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 286 266 192 125 101 85 46 45 29 20 BspEI T'CCGG_A Cuts at: 5244 5244 Size: 5581 BspHI T'CATG_A Cuts at: 3470 5200 3470 Size: 1730 3851 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2629 2936 844 Size: 1785 307 3489 Fragments arranged by size: 3489 1785 307 BsrBI CCG'CTC Cuts at: 1394 1823 2031 2557 2803 3468 1394 Size: 429 208 526 246 665 3507 Fragments arranged by size: 3507 665 526 429 246 208 BsrDI GCAATG_nn' Cuts at: 65 1248 4076 4250 65 Size: 1183 2828 174 1396 Fragments arranged by size: 2828 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2296 4225 4412 1700 Size: 110 486 1929 187 2869 Fragments arranged by size: 2869 1929 486 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5581 BssHII G'CGCG_C Cuts at: 1925 2379 2392 2394 2499 1925 Size: 454 13 2 105 5007 Fragments arranged by size: 5007 454 105 13 2 BssSI C'ACGA_G Cuts at: 2069 3641 4653 2069 Size: 1572 1012 2997 Fragments arranged by size: 2997 1572 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5581 BstBI TT'CG_AA Cuts at: 4385 4385 Size: 5581 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5581 BstXI CCAn_nnnn'nTGG Cuts at: 2173 2173 Size: 5581 BstYI r'GATC_y Cuts at: 1972 2911 3278 3663 3680 5132 5218 5230 5576 Size: 939 367 385 17 1452 86 12 346 Cuts at: 5576 1972 Size: 1977 Fragments arranged by size: 1977 1452 939 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2041 2061 2203 513 Size: 552 939 37 20 142 3891 Fragments arranged by size: 3891 939 552 142 37 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2687 487 Size: 683 56 1461 3381 Fragments arranged by size: 3381 1461 683 56 BtsI GCAGTG_nn' Cuts at: 3097 3891 3911 3097 Size: 794 20 4767 Fragments arranged by size: 4767 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5581 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5546 DraI TTT'AAA Cuts at: 2885 2885 Size: 5581 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4588 809 Size: 507 3272 1802 Fragments arranged by size: 3272 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2044 2574 2610 Size: 54 1425 50 275 18 214 530 36 Cuts at: 2610 3028 3917 5546 8 Size: 418 889 1629 43 Fragments arranged by size: 1629 1425 889 530 418 275 214 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2044 2574 2610 3028 1537 Size: 275 232 530 36 418 4090 Fragments arranged by size: 4090 530 418 275 232 36 EarI CTCTTCn'nnn_ Cuts at: 1353 3511 1353 Size: 2158 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4168 4542 4688 387 Size: 1064 138 2579 374 146 1280 Fragments arranged by size: 2579 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2107 2420 2456 2645 3644 4241 5028 Size: 393 475 313 36 189 999 597 787 Cuts at: 5028 5296 1239 Size: 268 1524 Fragments arranged by size: 1524 999 787 597 475 393 313 268 189 36 EcoO109I rG'GnC_Cy Cuts at: 2355 2414 2560 2704 2705 2355 Size: 59 146 144 1 5231 Fragments arranged by size: 5231 146 144 59 1 EcoRI G'AATT_C Cuts at: 2890 3268 2890 Size: 378 5203 FspI TGC'GCA Cuts at: 4087 4087 Size: 5581 HaeII r_GCGC'y Cuts at: 1678 2027 2069 2082 2097 2291 2303 2446 2681 Size: 349 42 13 15 194 12 143 235 Cuts at: 2681 2766 2850 4728 1678 Size: 85 84 1878 2531 Fragments arranged by size: 2531 1878 349 235 194 143 85 84 42 15 13 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2339 2371 4222 4254 4296 Size: 32 135 32 767 32 1851 32 42 Cuts at: 4296 4328 1373 Size: 32 2626 Fragments arranged by size: 2626 1851 767 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2925 3171 669 Size: 988 281 987 246 3079 Fragments arranged by size: 3079 988 987 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5581 HpaI GTT'AAC Cuts at: 3171 3171 Size: 5581 KasI G'GCGC_C Cuts at: 2023 2762 2023 Size: 739 4842 KpnI G_GTAC'C Cuts at: 2906 2906 Size: 5581 MfeI C'AATT_G Cuts at: 3180 3180 Size: 5581 MluI A'CGCG_T Cuts at: 5457 5457 Size: 5581 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4695 4879 1305 Size: 3390 184 2007 Fragments arranged by size: 3390 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3759 Fragments arranged by size: 3759 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3539 3898 4057 518 Size: 648 2373 359 159 2042 Fragments arranged by size: 2373 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2043 2205 2769 2997 3674 4822 5067 1606 Size: 437 162 564 228 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 564 437 245 228 162 NaeI GCC'GGC Cuts at: 2298 2298 Size: 5581 NarI GG'CG_CC Cuts at: 2024 2763 2024 Size: 739 4842 NcoI C'CATG_G Cuts at: 513 1065 2004 2061 513 Size: 552 939 57 4033 Fragments arranged by size: 4033 939 552 57 NdeI CA'TA_TG Cuts at: 387 387 Size: 5581 NgoMIV G'CCGG_C Cuts at: 2296 2296 Size: 5581 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5581 NotI GC'GGCC_GC Cuts at: 2044 2610 3028 2044 Size: 566 418 4597 Fragments arranged by size: 4597 566 418 NspI r_CATG'y Cuts at: 1132 1229 2939 4484 1132 Size: 97 1710 1545 2229 Fragments arranged by size: 2229 1710 1545 97 PasI CC'CwG_GG Cuts at: 1348 2019 1348 Size: 671 4910 PciI A'CATG_T Cuts at: 4480 4480 Size: 5581 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5581 PfoI T'CCnGG_A Cuts at: 2129 2129 Size: 5581 PmeI GTTT'AAAC Cuts at: 2885 2885 Size: 5581 PmlI CAC'GTG Cuts at: 2748 2748 Size: 5581 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3650 3682 5163 5195 3650 Size: 32 1481 32 4036 Fragments arranged by size: 4036 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2355 2355 Size: 5581 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5581 PsiI TTA'TAA Cuts at: 3151 3151 Size: 5581 PspOMI G'GGCC_C Cuts at: 2101 2413 2559 2704 2101 Size: 312 146 145 4978 Fragments arranged by size: 4978 312 146 145 PstI C_TGCA'G Cuts at: 830 2640 2933 830 Size: 1810 293 3478 Fragments arranged by size: 3478 1810 293 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5581 SacII CC_GC'GG Cuts at: 2044 2206 2044 Size: 162 5419 SalI G'TCGA_C Cuts at: 1936 2923 1936 Size: 987 4594 SanDI GG'GwC_CC Cuts at: 2355 2355 Size: 5581 SbfI CC_TGCA'GG Cuts at: 2933 2933 Size: 5581 ScaI AGT'ACT Cuts at: 2795 3829 5541 2795 Size: 1034 1712 2835 Fragments arranged by size: 2835 1712 1034 SfcI C'TryA_G Cuts at: 826 1045 2636 2929 4064 4745 4936 826 Size: 219 1591 293 1135 681 191 1471 Fragments arranged by size: 1591 1471 1135 681 293 219 191 SfoI GGC'GCC Cuts at: 2025 2764 2025 Size: 739 4842 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5581 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5581 SmaI CCC'GGG Cuts at: 1872 2100 2360 2908 1872 Size: 228 260 548 4545 Fragments arranged by size: 4545 548 260 228 SmlI C'TyrA_G Cuts at: 820 1017 1943 2699 3685 4586 4848 820 Size: 197 926 756 986 901 262 1553 Fragments arranged by size: 1553 986 926 901 756 262 197 SnaBI TAC'GTA Cuts at: 493 3372 493 Size: 2879 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5581 SphI G_CATG'C Cuts at: 1132 2939 1132 Size: 1807 3774 SspI AAT'ATT Cuts at: 5 52 3505 5 Size: 47 3453 2081 Fragments arranged by size: 3453 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5581 StyI C'CwwG_G Cuts at: 513 1065 2004 2061 3020 513 Size: 552 939 57 959 3074 Fragments arranged by size: 3074 959 939 552 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2705 3608 3625 3778 3963 4408 325 Size: 1255 1125 903 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1125 903 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2793 3827 5539 Size: 274 80 33 51 1443 816 1034 1712 Cuts at: 5539 96 Size: 138 Fragments arranged by size: 1712 1443 1034 816 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2582 2614 5541 5573 2582 Size: 32 2927 32 2590 Fragments arranged by size: 2927 2590 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5581 XbaI T'CTAG_A Cuts at: 2917 2917 Size: 5581 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5581 XmaI C'CCGG_G Cuts at: 1870 2098 2358 2906 1870 Size: 228 260 548 4545 Fragments arranged by size: 4545 548 260 228 XmnI GAAnn'nnTTC Cuts at: 3710 3710 Size: 5581 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3389 276 Size: 53 83 186 936 1855 2468 Fragments arranged by size: 2468 1855 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI XcmI