Restriction map for
pFN21AE1245
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI ApoI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbrPI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BseRI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI Eam1105I Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MflI
MluI MroI MseI MunI NcoI
NdeI NheI NruI NsiI NspI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I Van91I
XbaI XhoII
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# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Alw44I 537 GTGCAC G^TGCAC
ApaLI 537 GTGCAC G^TGCAC
AscI 615 GGCGCGCC GG^CGCGCC
Asp700I 1038 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 1165 CGACTGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 1333 CTGGAG CTGGAG(16/14)
BsaAI 700 TACGTG [CT]AC^GT[GA]
BsrBI 61 CCGCTC CCGCTC(-3/-3)
BstEII 40 GGTAACC G^GT[ACGT]ACC
BstF5I 579 CATCC GGATG(2/0)
BstPI 40 GGTAACC G^GT[ACGT]ACC
BstXI 347 CCACCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 527 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 540 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 527 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 40 GGTAACC G^GT[ACGT]ACC
EcoT14I 1424 CCAAGG C^C[AT][AT]GG
FokI 579 CATCC GGATG(9/13)
NotI 810 GCGGCCGC GC^GGCCGC
PaeR7I 1380 CTCGAG C^TCGAG
PleI 1378 GACTC GAGTC(4/5)
PstI 265 CTGCAG CTGCA^G
PvuII 777 CAGCTG CAG^CTG
SexAI 311 ACCTGGT A^CC[AT]GGT
SfcI 265 CTGCAG C^T[GA][CT]AG
SfiI 549 GGCCATCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1195 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1270 CCCGGG CCC^GGG
StyI 1424 CCAAGG C^C[AT][AT]GG
TfiI 1420 GAATC G^A[AT]TC
XhoI 1380 CTCGAG C^TCGAG
XmaCI 1270 CCCGGG C^CCGGG
XmaI 1270 CCCGGG C^CCGGG
XmnI 1038 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 375 GTAC GT^AC
729 GTAC
AlwNI 499 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1162 CAGCGACTG
AspHI 13 GTGCTC G[AT]GC[AT]^C
537 GTGCAC
BanII 34 GAGCCC G[GA]GC[CT]^C
1336 GAGCCC
BbeI 25 GGCGCC GGCGC^C
1199 GGCGCC
BbsI 994 GTCTTC GAAGAC(2/6)
1078 GTCTTC
BpuAI 994 GTCTTC GAAGAC(2/6)
1078 GTCTTC
BsaI 439 GAGACC GGTCTC(1/5)
1217 GAGACC
BsiHKAI 13 GTGCTC G[AT]GC[AT]^C
537 GTGCAC
BsmFI 294 GTCCC GGGAC(10/14)
838 GTCCC
BspMI 731 ACCTGC ACCTGC(4/8)
759 GCAGGT
BsrDI 453 CATTGC GCAATG(2/0)
224 GCAATG
BsrI 590 ACTGG ACTGG(1/-1)
1088 ACTGG
BssSI 532 CTCGTG CACGAG(-5/-1)
940 CTCGTG
DdeI 161 CTCAG C^T[ACGT]AG
405 CTCAG
DpnII 237 GATC ^GATC
771 GATC
DrdI 1294 GACGACGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1297 GACGATGTCGTC
EarI 283 CTCTTC CTCTTC(1/4)
1072 CTCTTC
HinfI 1378 GACTC G^A[ACGT]TC
1420 GAATC
KasI 25 GGCGCC G^GCGCC
1199 GGCGCC
Ksp632I 283 CTCTTC CTCTTC(1/4)
1072 CTCTTC
KspI 83 CCGCGG CCGC^GG
259 CCGCGG
MboI 237 GATC ^GATC
771 GATC
NaeI 1196 GCCGGC GCC^GGC
1345 GCCGGC
NarI 25 GGCGCC GG^CGCC
1199 GGCGCC
NdeII 237 GATC ^GATC
771 GATC
NgoMIV 1196 GCCGGC G^CCGGC
1345 GCCGGC
RsaI 375 GTAC GT^AC
729 GTAC
SacII 83 CCGCGG CCGC^GG
259 CCGCGG
Sau3AI 237 GATC ^GATC
771 GATC
SfoI 25 GGCGCC GGC^GCC
1199 GGCGCC
SmlI 967 CTCAAG C^T[CT][GA]AG
1380 CTCGAG
StuI 1040 AGGCCT AGG^CCT
1154 AGGCCT
Tsp45I 424 GTGAC ^GT[GC]AC
655 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AluI AspI
AvaI AvaII BalI BanI BbvI
BcnI BglI BmyI BsaHI BsaJI
BsiEI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrFI BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DsaI EaeI EagI EclXI Eco52I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MaeIII
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PflFI Sau96I
ScrFI SfaNI TaqI TseI TspRI
Tth111I TthHB8I XcmI