##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BmyI        BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HapII       HhaI        Hin1I       HinP1I      
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AhdI          142  GACCTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         142  GACCTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         516  GGACC                G^G[AT]CC
BanI          304  GGCACC               G^G[CT][GA]CC
BlpI           40  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       40  GCTCAGC              GC^T[ACGT]AGC
BsmBI         166  GAGACG               CGTCTC(1/5)
BsrDI         335  GCAATG               GCAATG(2/0)
BsrI          616  ACTGG                ACTGG(1/-1)
BssKI         576  CCAGG                ^CC[ACGT]GG
BstAPI        699  GCATTTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         576  CCAGG                CC^[AT]GG
CelII          40  GCTCAGC              GC^T[ACGT]AGC
Cfr13I        516  GGACC                G^G[ACGT]CC
Eam1105I      142  GACCTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII        576  CCAGG                ^CC[AT]GG
HaeIII        574  GGCC                 GG^CC
HgaI          168  GACGC                GACGC(5/10)
HincII        150  GTCAAC               GT[CT]^[GA]AC
HindII        150  GTCAAC               GT[CT]^[GA]AC
HindIII       467  AAGCTT               A^AGCTT
HinfI         348  GACTC                G^A[ACGT]TC
MvaI          576  CCAGG                CC^[AT]GG
MwoI          699  GCATTTGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI          348  GACTC                GAGTC(4/5)
PvuII          22  CAGCTG               CAG^CTG
Sau96I        516  GGACC                G^G[ACGT]CC
ScrFI         576  CCAGG                CC^[ACGT]GG
SfaNI         705  GATGC                GCATC(5/9)
SfcI          371  CTGTAG               C^T[GA][CT]AG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AciI          308  CCGC                 CCGC(-3/-1)
              274  GCGG                
BpmI           46  CTCCAG               CTGGAG(16/14)
              246  CTGGAG              
BseRI         283  GAGGAG               GAGGAG(10/8)
              585  GAGGAG              
BsmAI         166  GAGAC                GTCTC(1/5)
              227  GAGAC               
HphI          317  TCACC                GGTGA(8/7)
              601  TCACC               
MaeIII        346  GTGAC                ^GT[ACGT]AC
              613  GTGAC               
MspA1I         22  CAGCTG               C[AC]G^C[GT]G
              308  CCGCTG              
NlaIII        215  CATG                 CATG^
              417  CATG                
NlaIV         304  GGCACC               GG[ACGT]^[ACGT]CC
              634  GGAACC              
SmlI          364  CTCAAG               C^T[CT][GA]AG
              499  CTTGAG              
Tsp45I        346  GTGAC                ^GT[GC]AC
              613  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AluI        BbvI        Cac8I       DdeI        Fnu4HI      
ItaI        MboII       MnlI        TseI        Tsp509I     
TspRI