################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FseI FspI HgaI Hin1I KasI KpnI KspI MaeI MaeII MamI MfeI MluI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 955 GTCTAC GT^[AC][GT]AC AccIII 249 TCCGGA T^CCGGA AfaI 65 GTAC GT^AC Asp700I 768 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 345 GAAGAC GAAGAC(2/6) BclI 746 TGATCA T^GATCA BpuAI 345 GAAGAC GAAGAC(2/6) BsaWI 249 TCCGGA [AT]^CCGG[AT] BseAI 249 TCCGGA T^CCGGA BsgI 160 GTGCAG GTGCAG(16/14) BspEI 249 TCCGGA T^CCGGA BsrFI 175 GCCGGC [GA]^CCGG[CT] BstEII 183 GGTGACC G^GT[ACGT]ACC BstPI 183 GGTGACC G^GT[ACGT]ACC BstXI 488 CCACGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 923 AGATCC [GA]^GATC[CT] CfoI 783 GCGC GCG^C Cfr10I 175 GCCGGC [GA]^CCGG[CT] EarI 876 GAAGAG CTCTTC(1/4) EcoO65I 183 GGTGACC G^GT[ACGT]ACC FbaI 746 TGATCA T^GATCA HaeII 782 AGCGCC [GA]GCGC^[CT] HhaI 783 GCGC GCG^C HinP1I 783 GCGC G^CGC HincII 400 GTTAAC GT[CT]^[GA]AC HindII 400 GTTAAC GT[CT]^[GA]AC HindIII 143 AAGCTT A^AGCTT HpaI 400 GTTAAC GTT^AAC Ksp632I 876 GAAGAG CTCTTC(1/4) MflI 923 AGATCC [GA]^GATC[CT] MroI 249 TCCGGA T^CCGGA MseI 401 TTAA T^TAA NaeI 175 GCCGGC GCC^GGC NcoI 728 CCATGG C^CATGG NgoMIV 175 GCCGGC G^CCGGC RsaI 65 GTAC GT^AC SapI 876 GAAGAGC GCTCTTC(1/4) SbfI 807 CCTGCAGG CCTGCA^GG ScaI 64 AGTACT AGT^ACT SphI 326 GCATGC GCATG^C Sse8387I 807 CCTGCAGG CCTGCA^GG TaqI 111 TCGA T^CGA Tru9I 401 TTAA T^TAA Tsp509I 20 AATT ^AATT TthHB8I 111 TCGA T^CGA XhoII 923 AGATCC [GA]^GATC[CT] XmnI 768 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AspHI 879 GAGCTC G[AT]GC[AT]^C 917 GAGCTC BalI 485 TGGCCA TGG^CCA 731 TGGCCA BanI 297 GGTGCC G^G[CT][GA]CC 801 GGCACC BpmI 476 CTGGAG CTGGAG(16/14) 760 CTGGAG BsiHKAI 879 GAGCTC G[AT]GC[AT]^C 917 GAGCTC Cac8I 175 GCCGGC GC[ACGT]^[ACGT]GC 326 GCATGC DdeI 913 CTGAG C^T[ACGT]AG 920 CTCAG EcoT14I 530 CCTTGG C^C[AT][AT]GG 728 CCATGG MluNI 485 TGGCCA TGG^CCA 731 TGGCCA MscI 485 TGGCCA TGG^CCA 731 TGGCCA NspI 284 GCATGT [GA]CATG^[CT] 326 GCATGC PleI 235 GACTC GAGTC(4/5) 500 GACTC SacI 879 GAGCTC GAGCT^C 917 GAGCTC SfaNI 578 GCATC GCATC(5/9) 620 GCATC StyI 530 CCTTGG C^C[AT][AT]GG 728 CCATGG XcmI 473 CCACTGGAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 941 CCAGCGTTGAAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cfr13I DpnII DsaI EaeI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI PvuII Sau3AI Sau96I ScrFI SfcI TfiI TseI Tsp45I TspRI