(Circular) (Six-base) MAPSORT of: pFN21AE6357.seq Check: 3146 from: 1 to: 5680 pFN21AE6357 5680 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2535 2608 2806 2535 Size: 73 198 5409 Fragments arranged by size: 5409 198 73 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2961 3023 1937 Size: 1024 62 4594 Fragments arranged by size: 4594 1024 62 Acc65I G'GTAC_C Cuts at: 3001 3001 Size: 5680 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2804 3743 5127 5395 1632 Size: 1172 939 1384 268 1917 Fragments arranged by size: 1917 1384 1172 939 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5483 AflIII A'CryG_T Cuts at: 1203 4579 5556 1203 Size: 3376 977 1327 Fragments arranged by size: 3376 1327 977 AgeI A'CCGG_T Cuts at: 4511 4511 Size: 5680 AhdI GACnn_n'nnGTC Cuts at: 4409 4409 Size: 5680 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5680 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2567 1184 Size: 1383 4297 AlwNI CAG_nnn'CTG Cuts at: 3423 4995 3423 Size: 1572 4108 ApaLI G'TGCA_C Cuts at: 3737 4893 3737 Size: 1156 4524 ApoI r'AATT_y Cuts at: 1493 2989 3215 3367 5476 1493 Size: 1496 226 152 2109 1697 Fragments arranged by size: 2109 1697 1496 226 152 AseI AT'TA_AT Cuts at: 160 1032 4234 160 Size: 872 3202 1606 Fragments arranged by size: 3202 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 3005 1870 Size: 73 1062 4545 Fragments arranged by size: 4545 1062 73 BamHI G'GATC_C Cuts at: 3010 3377 3010 Size: 367 5313 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2302 2806 3001 4456 618 Size: 325 217 627 515 504 195 1455 1842 Fragments arranged by size: 1842 1455 627 515 504 325 217 195 BanII G_rGCy'C Cuts at: 1391 2089 2888 2926 2999 1391 Size: 698 799 38 73 4072 Fragments arranged by size: 4072 799 698 73 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2357 928 Size: 971 458 4251 Fragments arranged by size: 4251 971 458 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3871 3905 1903 Size: 34 1934 34 3678 Fragments arranged by size: 3678 1934 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3572 4782 3572 Size: 1210 4470 BclI T'GATC_A Cuts at: 1548 2751 1548 Size: 1203 4477 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4291 136 Size: 107 122 71 631 605 2619 1525 Fragments arranged by size: 2619 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5675 5675 Size: 5680 BlpI GC'TnA_GC Cuts at: 3097 3097 Size: 5680 Bme1580I G_kGCm'C Cuts at: 1790 2140 2307 2556 2809 2869 3741 4897 1790 Size: 350 167 249 253 60 872 1156 2573 Fragments arranged by size: 2573 1156 872 350 253 249 167 60 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5680 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3411 4369 442 Size: 416 53 83 399 24 1994 958 1753 Fragments arranged by size: 1994 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2502 2786 4340 1239 Size: 1263 284 1554 2579 Fragments arranged by size: 2579 1554 1263 284 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3805 4670 4968 3805 Size: 865 298 4517 Fragments arranged by size: 4517 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4343 882 Size: 617 2844 2219 Fragments arranged by size: 2844 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3471 493 Size: 711 2267 2702 Fragments arranged by size: 2702 2267 711 BsaBI GATnn'nnATC Cuts at: 5674 5674 Size: 5680 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3487 3869 4504 275 Size: 53 83 186 936 1954 382 635 1451 Fragments arranged by size: 1954 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2254 4113 4511 4785 4932 5343 2254 Size: 1859 398 274 147 411 2591 Fragments arranged by size: 2591 1859 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5455 Fragments arranged by size: 5455 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5680 BseYI C'CCAG_C Cuts at: 2058 2452 2800 2945 4883 5379 2058 Size: 394 348 145 1938 496 2359 Fragments arranged by size: 2359 1938 496 394 348 145 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 1163 Size: 573 450 4657 Fragments arranged by size: 4657 573 450 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3130 3891 4919 1540 Size: 275 186 1129 761 1028 2301 Fragments arranged by size: 2301 1129 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2888 2926 3741 3826 4897 2888 Size: 38 815 85 1071 3671 Fragments arranged by size: 3671 1071 815 85 38 BsmI GAATG_Cn' Cuts at: 3191 3284 3191 Size: 93 5587 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2089 2140 2307 2556 2809 2869 2888 Size: 399 299 51 167 249 253 60 19 Cuts at: 2888 2926 2999 3741 3826 4897 1391 Size: 38 73 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 299 253 249 167 85 73 60 51 38 19 BspEI T'CCGG_A Cuts at: 2254 5343 2254 Size: 3089 2591 BspHI T'CATG_A Cuts at: 3569 5299 3569 Size: 1730 3950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2535 2608 2636 2806 3035 844 Size: 1691 73 28 170 229 3489 Fragments arranged by size: 3489 1691 229 170 73 28 BsrBI CCG'CTC Cuts at: 1394 1823 3567 1394 Size: 429 1744 3507 Fragments arranged by size: 3507 1744 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4175 4349 65 Size: 1183 2927 174 1396 Fragments arranged by size: 2927 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2180 4324 4511 1700 Size: 110 370 2144 187 2869 Fragments arranged by size: 2869 2144 370 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5680 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5680 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5680 BstBI TT'CG_AA Cuts at: 4484 4484 Size: 5680 BstEII G'GTnAC_C Cuts at: 1184 2188 1184 Size: 1004 4676 BstXI CCAn_nnnn'nTGG Cuts at: 2500 2500 Size: 5680 BstYI r'GATC_y Cuts at: 1972 2928 3010 3377 3762 3779 5231 5317 5329 Size: 956 82 367 385 17 1452 86 12 Cuts at: 5329 5675 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 956 385 367 346 86 82 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2361 2493 2733 513 Size: 552 939 357 132 240 3460 Fragments arranged by size: 3460 939 552 357 240 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4941 Fragments arranged by size: 4941 683 56 BtsI GCAGTG_nn' Cuts at: 2381 2428 2571 3196 3990 4010 2381 Size: 47 143 625 794 20 4051 Fragments arranged by size: 4051 794 625 143 47 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2404 2439 529 Size: 35 1840 35 3770 Fragments arranged by size: 3770 1840 35 35 DraI TTT'AAA Cuts at: 2984 2984 Size: 5680 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4687 809 Size: 507 3371 1802 Fragments arranged by size: 3371 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2284 2490 2736 Size: 54 1425 50 275 18 454 206 246 Cuts at: 2736 3127 4016 5645 8 Size: 391 889 1629 43 Fragments arranged by size: 1629 1425 889 454 391 275 246 206 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3127 1537 Size: 275 1315 4090 Fragments arranged by size: 4090 1315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2876 3610 1353 Size: 1523 734 3423 Fragments arranged by size: 3423 1523 734 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2233 4267 4641 4787 387 Size: 1064 138 644 2034 374 146 1280 Fragments arranged by size: 2034 1280 1064 644 374 146 138 EcoICRI GAG'CTC Cuts at: 2886 2924 2886 Size: 38 5642 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2502 2786 2804 3743 4340 5127 5395 Size: 393 870 284 18 939 597 787 268 Cuts at: 5395 1239 Size: 1524 Fragments arranged by size: 1524 939 870 787 597 393 284 268 18 EcoRI G'AATT_C Cuts at: 2989 3367 2989 Size: 378 5302 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2794 2794 Size: 5680 FspI TGC'GCA Cuts at: 4186 4186 Size: 5680 HaeII r_GCGC'y Cuts at: 1678 2791 4827 1678 Size: 1113 2036 2531 Fragments arranged by size: 2531 2036 1113 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2913 2945 4321 4353 4395 Size: 32 135 32 1341 32 1376 32 42 Cuts at: 4395 4427 1373 Size: 32 2626 Fragments arranged by size: 2626 1376 1341 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2407 3024 3270 669 Size: 988 281 469 617 246 3079 Fragments arranged by size: 3079 988 617 469 281 246 HindIII A'AGCT_T Cuts at: 748 2148 748 Size: 1400 4280 HpaI GTT'AAC Cuts at: 2407 3270 2407 Size: 863 4817 KpnI G_GTAC'C Cuts at: 3005 3005 Size: 5680 MfeI C'AATT_G Cuts at: 3279 3279 Size: 5680 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2439 4794 4978 1305 Size: 1134 2355 184 2007 Fragments arranged by size: 2355 2007 1134 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2492 2738 10 Size: 54 1425 343 660 246 2952 Fragments arranged by size: 2952 1425 660 343 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 2312 2441 2630 3638 3997 4156 518 Size: 648 1146 129 189 1008 359 159 2042 Fragments arranged by size: 2042 1146 1008 648 359 189 159 129 MspA1I CmG'CkG Cuts at: 1606 2143 2215 2452 2573 3096 3773 4921 5166 Size: 537 72 237 121 523 677 1148 245 Cuts at: 5166 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 537 523 245 237 121 72 NaeI GCC'GGC Cuts at: 2182 2182 Size: 5680 NcoI C'CATG_G Cuts at: 513 1065 2004 2733 513 Size: 552 939 729 3460 Fragments arranged by size: 3460 939 729 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NgoMIV G'CCGG_C Cuts at: 2180 2180 Size: 5680 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5680 NotI GC'GGCC_GC Cuts at: 3127 3127 Size: 5680 NspI r_CATG'y Cuts at: 1132 1229 2293 2335 3038 4583 1132 Size: 97 1064 42 703 1545 2229 Fragments arranged by size: 2229 1545 1064 703 97 42 PasI CC'CwG_GG Cuts at: 1348 2195 2500 1348 Size: 847 305 4528 Fragments arranged by size: 4528 847 305 PciI A'CATG_T Cuts at: 4579 4579 Size: 5680 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5680 PfoI T'CCnGG_A Cuts at: 2046 2046 Size: 5680 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3781 5262 5294 3749 Size: 32 1481 32 4135 Fragments arranged by size: 4135 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5680 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PstI C_TGCA'G Cuts at: 830 2619 2677 2817 3032 830 Size: 1789 58 140 215 3478 Fragments arranged by size: 3478 1789 215 140 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5680 PvuII CAG'CTG Cuts at: 2143 2215 2452 2573 2143 Size: 72 237 121 5250 Fragments arranged by size: 5250 237 121 72 SacI G_AGCT'C Cuts at: 2888 2926 2888 Size: 38 5642 SalI G'TCGA_C Cuts at: 1936 3022 1936 Size: 1086 4594 SapI GCTCTTCn'nnn_ Cuts at: 2876 2876 Size: 5680 SbfI CC_TGCA'GG Cuts at: 2817 3032 2817 Size: 215 5465 ScaI AGT'ACT Cuts at: 2071 3928 5640 2071 Size: 1857 1712 2111 Fragments arranged by size: 2111 1857 1712 SfcI C'TryA_G Cuts at: 826 1045 2173 2344 2615 2673 2813 3028 4163 Size: 219 1128 171 271 58 140 215 1135 Cuts at: 4163 4844 5035 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1128 681 271 219 215 191 171 140 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5680 SmaI CCC'GGG Cuts at: 1872 3007 1872 Size: 1135 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3784 4685 4947 820 Size: 197 926 1841 901 262 1553 Fragments arranged by size: 1841 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 2335 3038 1132 Size: 1203 703 3774 Fragments arranged by size: 3774 1203 703 SspI AAT'ATT Cuts at: 5 52 3604 5 Size: 47 3552 2081 Fragments arranged by size: 3552 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5680 StyI C'CwwG_G Cuts at: 513 1065 2004 2535 2733 3119 513 Size: 552 939 531 198 386 3074 Fragments arranged by size: 3074 939 552 531 386 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2009 2368 3707 3724 3877 4062 4507 Size: 1255 429 359 1339 17 153 185 445 Cuts at: 4507 325 Size: 1498 Fragments arranged by size: 1498 1339 1255 445 429 359 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2069 3926 5638 Size: 274 80 33 51 1443 92 1857 1712 Cuts at: 5638 96 Size: 138 Fragments arranged by size: 1857 1712 1443 274 138 92 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2201 2233 5640 5672 2201 Size: 32 3407 32 2209 Fragments arranged by size: 3407 2209 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5680 XbaI T'CTAG_A Cuts at: 3016 3016 Size: 5680 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2485 2953 2485 Size: 468 5212 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5680 XmaI C'CCGG_G Cuts at: 1870 3005 1870 Size: 1135 4545 XmnI GAAnn'nnTTC Cuts at: 2777 3809 2777 Size: 1032 4648 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI