################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AfaI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 678 GTCTAC GT^[AC][GT]AC AflIII 501 ACGCGT A^C[GA][CT]GT AvaI 690 CTCGGG C^[CT]CG[GA]G BamHI 632 GGATCC G^GATCC BciVI 41 GGATAC GTATCC(6/5) BcnI 50 CCGGG CC^[GC]GG BfaI 683 CTAG C^TAG BpmI 472 CTGGAG CTGGAG(16/14) BsaI 475 GAGACC GGTCTC(1/5) BsmFI 393 GTCCC GGGAC(10/14) BsoBI 690 CTCGGG C^[CT]CG[GA]G BspHI 453 TCATGA T^CATGA BstBI 704 TTCGAA TT^CGAA Cac8I 570 GCAGGC GC[ACGT]^[ACGT]GC CfoI 37 GCGC GCG^C Cfr13I 14 GGGCC G^G[ACGT]CC DraII 13 GGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 247 CGGCCA [CT]^GGCC[GA] EcoO109I 13 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 658 GAATTC G^AATTC HhaI 37 GCGC GCG^C HinP1I 37 GCGC G^CGC HincII 507 GTTGAC GT[CT]^[GA]AC HindII 507 GTTGAC GT[CT]^[GA]AC HindIII 319 AAGCTT A^AGCTT MaeI 683 CTAG C^TAG MboII 361 GAAGA GAAGA(8/7) MluI 501 ACGCGT A^CGCGT MseI 484 TTAA T^TAA MslI 810 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 110 CAGCTG C[AC]G^C[GT]G NciI 50 CCGGG CC^[GC]GG NcoI 722 CCATGG C^CATGG NspV 704 TTCGAA TT^CGAA PflMI 598 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 304 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 110 CAGCTG CAG^CTG RcaI 453 TCATGA T^CATGA Sau96I 14 GGGCC G^G[ACGT]CC SfcI 653 CTACAG C^T[GA][CT]AG SfuI 704 TTCGAA TT^CGAA SmlI 637 CTTGAG C^T[CT][GA]AG SspI 749 AATATT AAT^ATT TaqI 705 TCGA T^CGA Tru9I 484 TTAA T^TAA TthHB8I 705 TCGA T^CGA Van91I 598 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 38 CGCG CG^CG 502 CGCG AciI 411 CCGC CCGC(-3/-1) 39 GCGG AcsI 658 GAATTC [GA]^AATT[CT] 708 AAATTC ApoI 658 GAATTC [GA]^AATT[CT] 708 AAATTC BbvI 64 GCAGC GCAGC(8/12) 466 GCAGC BseRI 18 CTCCTC GAGGAG(10/8) 128 GAGGAG BsmAI 475 GAGAC GTCTC(1/5) 686 GAGAC BsmI 117 GAATGC GAATGC(1/-1) 792 GAATGC BstF5I 810 CATCC GGATG(2/0) 517 GGATG BstNI 598 CCAGG CC^[AT]GG 813 CCTGG BstUI 38 CGCG CG^CG 502 CGCG BstYI 224 AGATCC [GA]^GATC[CT] 632 GGATCC DdeI 23 CTGAG C^T[ACGT]AG 744 CTCAG DsaI 250 CCACGG C^C[GA][CT]GG 722 CCATGG EcoRII 598 CCAGG ^CC[AT]GG 813 CCTGG EcoT14I 488 CCTTGG C^C[AT][AT]GG 722 CCATGG FokI 810 CATCC GGATG(9/13) 517 GGATG HapII 50 CCGG C^CGG 59 CCGG HpaII 50 CCGG C^CGG 59 CCGG HphI 259 GGTGA GGTGA(8/7) 291 GGTGA MflI 224 AGATCC [GA]^GATC[CT] 632 GGATCC MspI 50 CCGG C^CGG 59 CCGG MvaI 598 CCAGG CC^[AT]GG 813 CCTGG MvnI 38 CGCG CG^CG 502 CGCG PleI 560 GACTC GAGTC(4/5) 688 GACTC StyI 488 CCTTGG C^C[AT][AT]GG 722 CCATGG TseI 64 GCAGC G^C[AT]GC 466 GCAGC XhoII 224 AGATCC [GA]^GATC[CT] 632 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AluI AlwI BsaJI BsiYI BslI BsrI BssKI DpnII Fnu4HI HaeIII HinfI ItaI MaeIII MboI MnlI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI TfiI Tsp45I Tsp509I TspRI