################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AccI AccIII AclI AcsI AflII AflIII AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AviII AvrII BalI BamHI BbrPI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV FseI FspI KspI MamI MluI MluNI MroI MscI NaeI NdeI NgoMIV NheI NruI NspV PacI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 484 GACGTC GACGT^C Acc65I 539 GGTACC G^GTACC AgeI 1341 ACCGGT A^CCGGT AocI 1170 CCTGAGG CC^T[ACGT]AGG Asp718I 539 GGTACC G^GTACC AvaI 836 CTCGAG C^[CT]CG[GA]G BbsI 469 GTCTTC GAAGAC(2/6) BbvCI 1293 GCTGAGG CCTCAGC(-5/-2) BcgI 907 GCAAACCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1114 CTAG C^TAG BglI 160 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 469 GTCTTC GAAGAC(2/6) BsoBI 836 CTCGAG C^[CT]CG[GA]G BsrBI 48 CCGCTC CCGCTC(-3/-3) BsrFI 1341 ACCGGT [GA]^CCGG[CT] BstAPI 1064 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 443 GGATCT [GA]^GATC[CT] Bsu36I 1170 CCTGAGG CC^T[ACGT]AGG Cfr10I 1341 ACCGGT [GA]^CCGG[CT] DsaI 479 CCATGG C^C[GA][CT]GG EagI 21 CGGCCG C^GGCCG EclXI 21 CGGCCG C^GGCCG Eco52I 21 CGGCCG C^GGCCG Eco81I 1170 CCTGAGG CC^T[ACGT]AGG EcoNI 1301 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 10 GTTAAC GT[CT]^[GA]AC HindII 10 GTTAAC GT[CT]^[GA]AC HindIII 1258 AAGCTT A^AGCTT HpaI 10 GTTAAC GTT^AAC KpnI 539 GGTACC GGTAC^C MaeI 1114 CTAG C^TAG MfeI 1380 CAATTG C^AATTG MflI 443 GGATCT [GA]^GATC[CT] MunI 1380 CAATTG C^AATTG NcoI 479 CCATGG C^CATGG NotI 20 GCGGCCGC GC^GGCCGC NspI 1241 GCATGC [GA]CATG^[CT] PaeR7I 836 CTCGAG C^TCGAG PflMI 649 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1341 ACCGGT A^CCGGT PpuMI 1166 GGGACCT [GA]G^G[AT]CC[CT] PvuI 284 CGATCG CGAT^CG SfaNI 1079 GCATC GCATC(5/9) SphI 1241 GCATGC GCATG^C Tsp509I 1381 AATT ^AATT Van91I 649 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 649 CCACTCTTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 836 CTCGAG C^TCGAG XhoII 443 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AspI 112 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 850 GACAGCGTC BclI 524 TGATCA T^GATCA 695 TGATCA BsaI 1278 GAGACC GGTCTC(1/5) 1323 GAGACC BseRI 922 CTCCTC GAGGAG(10/8) 1033 CTCCTC BsgI 495 CTGCAC GTGCAG(16/14) 1302 CTGCAC BsrI 1197 CCAGT ACTGG(1/-1) 1203 CCAGT BstXI 702 CCAGGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 977 CCACCACCCTGG EaeI 21 CGGCCG [CT]^GGCC[GA] 374 TGGCCG EarI 801 GAAGAG CTCTTC(1/4) 898 GAAGAG EcoT14I 479 CCATGG C^C[AT][AT]GG 543 CCTTGG EcoT22I 591 ATGCAT ATGCA^T 1243 ATGCAT FbaI 524 TGATCA T^GATCA 695 TGATCA Ksp632I 801 GAAGAG CTCTTC(1/4) 898 GAAGAG MaeII 8 ACGT A^CGT 485 ACGT MaeIII 154 GTGAC ^GT[ACGT]AC 418 GTCAC MslI 474 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1103 CATTTCTGTG NsiI 591 ATGCAT ATGCA^T 1243 ATGCAT PflFI 112 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 850 GACAGCGTC PleI 920 GACTC GAGTC(4/5) 233 GAGTC PstI 1071 CTGCAG CTGCA^G 1235 CTGCAG PvuII 1074 CAGCTG CAG^CTG 1232 CAGCTG StyI 479 CCATGG C^C[AT][AT]GG 543 CCTTGG TfiI 606 GATTC G^A[AT]TC 1428 GATTC Tsp45I 154 GTGAC ^GT[GC]AC 418 GTCAC Tth111I 112 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 850 GACAGCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaII BanI BanII BbeI BbvI BcnI BmyI BpmI BsaHI BsaJI BsaWI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV RcaI RsaI Sau3AI Sau96I ScrFI SfcI SfoI SmlI TaqI Tru9I TseI TspRI TthHB8I