(Circular) (Six-base) MAPSORT of: pFN21AE9563.seq Check: 8254 from: 1 to: 6142 pFN21AE9563 6142 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2493 3952 278 Size: 53 83 186 936 957 1459 2468 Fragments arranged by size: 2468 1459 957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3485 1937 Size: 1548 4594 Acc65I G'GTAC_C Cuts at: 2544 3463 2544 Size: 919 5223 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3281 4205 5589 5857 1632 Size: 1649 924 1384 268 1917 Fragments arranged by size: 1917 1649 1384 924 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 5041 6018 1203 Size: 3838 977 1327 Fragments arranged by size: 3838 1327 977 AgeI A'CCGG_T Cuts at: 3346 4973 3346 Size: 1627 4515 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6142 AlwNI CAG_nnn'CTG Cuts at: 3885 5457 3885 Size: 1572 4570 ApaLI G'TGCA_C Cuts at: 4199 5355 4199 Size: 1156 4986 ApoI r'AATT_y Cuts at: 1493 3451 3677 3829 5938 1493 Size: 1958 226 152 2109 1697 Fragments arranged by size: 2109 1958 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 2841 3467 1870 Size: 73 898 626 4545 Fragments arranged by size: 4545 898 626 73 BamHI G'GATC_C Cuts at: 3472 3839 3472 Size: 367 5775 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2046 2111 2137 2173 2544 Size: 325 217 627 259 65 26 36 371 Cuts at: 2544 3463 4918 618 Size: 919 1455 1842 Fragments arranged by size: 1842 1455 919 627 371 325 259 217 65 36 26 BanII G_rGCy'C Cuts at: 1391 2102 2132 2671 3461 1391 Size: 711 30 539 790 4072 Fragments arranged by size: 4072 790 711 539 30 BbeI G_GCGC'C Cuts at: 2115 2141 2177 2115 Size: 26 36 6080 Fragments arranged by size: 6080 36 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2467 928 Size: 971 568 4603 Fragments arranged by size: 4603 971 568 BbvCI CC'TCA_GC Cuts at: 3299 3299 Size: 6142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2901 2935 4333 4367 1903 Size: 34 964 34 1398 34 3678 Fragments arranged by size: 3678 1398 964 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 2529 2700 1548 Size: 981 171 4990 Fragments arranged by size: 4990 981 171 BfrBI ATG'CAT Cuts at: 2598 3250 2598 Size: 652 5490 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2171 4753 136 Size: 107 122 71 631 605 499 2582 1525 Fragments arranged by size: 2582 1525 631 605 499 122 107 71 BglII A'GATC_T Cuts at: 6137 6137 Size: 6142 BlpI GC'TnA_GC Cuts at: 3559 3559 Size: 6142 Bme1580I G_kGCm'C Cuts at: 1790 2049 4203 5359 1790 Size: 259 2154 1156 2573 Fragments arranged by size: 2573 2154 1156 259 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6142 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3873 4831 442 Size: 416 53 83 399 24 2456 958 1753 Fragments arranged by size: 2456 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2873 3185 3357 4802 1239 Size: 1634 312 172 1445 2579 Fragments arranged by size: 2579 1634 1445 312 172 Bpu10I CC'TnA_GC Cuts at: 3299 3299 Size: 6142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2116 2915 4267 5132 5430 2116 Size: 799 1352 865 298 2828 Fragments arranged by size: 2828 1352 865 799 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3277 3322 4805 882 Size: 617 1778 45 1483 2219 Fragments arranged by size: 2219 1778 1483 617 45 BsaAI yAC'GTr Cuts at: 493 1204 3933 493 Size: 711 2729 2702 Fragments arranged by size: 2729 2702 711 BsaBI GATnn'nnATC Cuts at: 6136 6136 Size: 6142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2112 2138 2174 2490 Size: 53 83 186 936 579 26 36 316 Cuts at: 2490 3949 4331 4966 275 Size: 1459 382 635 1451 Fragments arranged by size: 1459 1451 936 635 579 382 316 186 83 53 36 26 BsaWI w'CCGG_w Cuts at: 3213 3225 3346 4575 4973 5247 5394 5805 3213 Size: 12 121 1229 398 274 147 411 3550 Fragments arranged by size: 3550 1229 411 398 274 147 121 12 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2189 2219 2635 2665 1178 Size: 30 165 30 786 30 416 30 4655 Fragments arranged by size: 4655 786 416 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2918 3029 1186 Size: 1732 111 4299 Fragments arranged by size: 4299 1732 111 BseYI C'CCAG_C Cuts at: 5345 5841 5345 Size: 496 5646 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2485 3292 1163 Size: 573 749 807 4013 Fragments arranged by size: 4013 807 749 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2029 2124 2292 3592 4353 5381 Size: 275 186 28 95 168 1300 761 1028 Cuts at: 5381 1540 Size: 2301 Fragments arranged by size: 2301 1300 1028 761 275 186 168 95 28 BsiHKAI G_wGCw'C Cuts at: 4203 4288 5359 4203 Size: 85 1071 4986 Fragments arranged by size: 4986 1071 85 BsmI GAATG_Cn' Cuts at: 3653 3746 3653 Size: 93 6049 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2102 2132 2671 3461 4203 4288 Size: 399 259 53 30 539 790 742 85 Cuts at: 4288 5359 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 790 742 539 399 259 85 53 30 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 2514 3429 3436 4031 5761 2514 Size: 915 7 595 1730 2895 Fragments arranged by size: 2895 1730 915 595 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3497 844 Size: 2653 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2055 4029 1394 Size: 429 232 1974 3507 Fragments arranged by size: 3507 1974 429 232 BsrDI GCAATG_nn' Cuts at: 65 1248 4637 4811 65 Size: 1183 3389 174 1396 Fragments arranged by size: 3389 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3346 4786 4973 1700 Size: 110 1536 1440 187 2869 Fragments arranged by size: 2869 1536 1440 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 3075 794 Size: 2281 3861 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6142 BstXI CCAn_nnnn'nTGG Cuts at: 2714 2989 2714 Size: 275 5867 BstYI r'GATC_y Cuts at: 1972 2448 3472 3839 4224 4241 5693 5779 5791 Size: 476 1024 367 385 17 1452 86 12 Cuts at: 5791 6137 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1024 476 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3176 3176 Size: 6142 BtgI C'CryG_G Cuts at: 513 1065 2484 513 Size: 552 1419 4171 Fragments arranged by size: 4171 1419 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2464 2848 487 Size: 683 56 1238 384 3781 Fragments arranged by size: 3781 1238 683 384 56 BtsI GCAGTG_nn' Cuts at: 2745 3658 4452 4472 2745 Size: 913 794 20 4415 Fragments arranged by size: 4415 913 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5149 809 Size: 507 3833 1802 Fragments arranged by size: 3833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2026 2379 3589 Size: 54 1425 50 275 18 196 353 1210 Cuts at: 3589 4478 6107 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1210 889 353 275 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2026 3589 1537 Size: 275 214 1563 4090 Fragments arranged by size: 4090 1563 275 214 EarI CTCTTCn'nnn_ Cuts at: 1353 2801 2898 4072 1353 Size: 1448 97 1174 3423 Fragments arranged by size: 3423 1448 1174 97 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2330 2623 4729 5103 5249 387 Size: 1064 138 741 293 2106 374 146 1280 Fragments arranged by size: 2106 1280 1064 741 374 293 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2873 3185 3281 3357 4205 4802 5589 Size: 393 1241 312 96 76 848 597 787 Cuts at: 5589 5857 1239 Size: 268 1524 Fragments arranged by size: 1524 1241 848 787 597 393 312 268 96 76 EcoNI CCTnn'n_nnAGG Cuts at: 3310 3310 Size: 6142 EcoO109I rG'GnC_Cy Cuts at: 2562 3006 3172 3303 2562 Size: 444 166 131 5401 Fragments arranged by size: 5401 444 166 131 EcoRI G'AATT_C Cuts at: 3451 3829 3451 Size: 378 5764 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2467 2713 2467 Size: 246 5896 FspI TGC'GCA Cuts at: 4648 4648 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 2115 2141 2177 2378 5289 1678 Size: 437 26 36 201 2911 2531 Fragments arranged by size: 2911 2531 437 201 36 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2189 2221 3045 3077 3172 Size: 32 135 32 617 32 824 32 95 Cuts at: 3172 3204 4783 4815 4857 4889 1373 Size: 32 1579 32 42 32 2626 Fragments arranged by size: 2626 1579 824 617 135 95 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2017 3486 3732 669 Size: 988 281 79 1469 246 3079 Fragments arranged by size: 3079 1469 988 281 246 79 HindIII A'AGCT_T Cuts at: 748 3263 748 Size: 2515 3627 HpaI GTT'AAC Cuts at: 2017 3732 2017 Size: 1715 4427 KasI G'GCGC_C Cuts at: 2111 2137 2173 2111 Size: 26 36 6080 Fragments arranged by size: 6080 36 26 KpnI G_GTAC'C Cuts at: 2548 3467 2548 Size: 919 5223 MfeI C'AATT_G Cuts at: 3385 3741 3385 Size: 356 5786 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2376 5256 5440 1305 Size: 1071 2880 184 2007 Fragments arranged by size: 2880 2007 1071 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4320 Fragments arranged by size: 4320 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2483 3112 4100 4459 4618 518 Size: 648 1317 629 988 359 159 2042 Fragments arranged by size: 2042 1317 988 648 629 359 159 MspA1I CmG'CkG Cuts at: 1606 2025 3081 3239 3558 4235 5383 5628 1606 Size: 419 1056 158 319 677 1148 245 2120 Fragments arranged by size: 2120 1148 1056 677 419 319 245 158 NarI GG'CG_CC Cuts at: 2112 2138 2174 2112 Size: 26 36 6080 Fragments arranged by size: 6080 36 26 NcoI C'CATG_G Cuts at: 513 1065 2484 513 Size: 552 1419 4171 Fragments arranged by size: 4171 1419 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6142 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6142 NotI GC'GGCC_GC Cuts at: 2026 3589 2026 Size: 1563 4579 NsiI A_TGCA'T Cuts at: 2600 3252 2600 Size: 652 5490 NspI r_CATG'y Cuts at: 1132 1229 3250 3500 5045 1132 Size: 97 2021 250 1545 2229 Fragments arranged by size: 2229 2021 1545 250 97 PasI CC'CwG_GG Cuts at: 1348 2989 1348 Size: 1641 4501 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 2660 1108 Size: 1552 4590 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3172 3172 Size: 6142 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PspXI vC'TCGA_Gb Cuts at: 2841 2841 Size: 6142 PstI C_TGCA'G Cuts at: 830 3080 3244 3494 830 Size: 2250 164 250 3478 Fragments arranged by size: 3478 2250 250 164 PvuI CG_AT'CG Cuts at: 2001 2292 2001 Size: 291 5851 PvuII CAG'CTG Cuts at: 3081 3239 3081 Size: 158 5984 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 4390 6102 4390 Size: 1712 4430 SfcI C'TryA_G Cuts at: 826 1045 3076 3240 3414 3490 4625 5306 5497 Size: 219 2031 164 174 76 1135 681 191 Cuts at: 5497 826 Size: 1471 Fragments arranged by size: 2031 1471 1135 681 219 191 174 164 76 SfoI GGC'GCC Cuts at: 2113 2139 2175 2113 Size: 26 36 6080 Fragments arranged by size: 6080 36 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 3469 1872 Size: 1597 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2095 2841 2930 4246 5147 5409 Size: 197 926 152 746 89 1316 901 262 Cuts at: 5409 820 Size: 1553 Fragments arranged by size: 1553 1316 926 901 746 262 197 152 89 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 3250 3500 1132 Size: 2118 250 3774 Fragments arranged by size: 3774 2118 250 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6142 StyI C'CwwG_G Cuts at: 513 1065 2484 2548 3581 513 Size: 552 1419 64 1033 3074 Fragments arranged by size: 3074 1419 1033 552 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3085 3349 4169 4186 4339 4524 4969 Size: 1255 1505 264 820 17 153 185 445 Cuts at: 4969 325 Size: 1498 Fragments arranged by size: 1505 1498 1255 820 445 264 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2778 4388 6100 Size: 274 80 33 51 1443 801 1610 1712 Cuts at: 6100 96 Size: 138 Fragments arranged by size: 1712 1610 1443 801 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2480 2512 2635 2667 6102 6134 2480 Size: 32 123 32 3435 32 2488 Fragments arranged by size: 3435 2488 123 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2120 2858 1316 Size: 804 738 4600 Fragments arranged by size: 4600 804 738 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2661 2661 Size: 6142 XhoI C'TCGA_G Cuts at: 1943 2841 1943 Size: 898 5244 XmaI C'CCGG_G Cuts at: 1870 3467 1870 Size: 1597 4545 XmnI GAAnn'nnTTC Cuts at: 4271 4271 Size: 6142 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2491 3950 276 Size: 53 83 186 936 957 1459 2468 Fragments arranged by size: 2468 1459 957 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI