################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AccII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AspEI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI HinP1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI TaqI Tth111I TthHB8I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 1054 GACGTC GACGT^C Acc65I 730 GGTACC G^GTACC AccI 1048 GTCTAC GT^[AC][GT]AC AccIII 1107 TCCGGA T^CCGGA AcyI 1054 GACGTC G[GA]^CG[CT]C AseI 1174 ATTAAT AT^TAAT AsnI 1174 ATTAAT AT^TAAT Asp700I 694 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 730 GGTACC G^GTACC AspHI 763 GAGCTC G[AT]GC[AT]^C BamHI 87 GGATCC G^GATCC BanII 763 GAGCTC G[GA]GC[CT]^C BbsI 244 GTCTTC GAAGAC(2/6) BbvI 305 GCAGC GCAGC(8/12) BcgI 1032 CGACTGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 763 GAGCTC G[AGT]GC[ACT]^C BpuAI 244 GTCTTC GAAGAC(2/6) BsaHI 1054 GACGTC G[GA]^CG[CT]C BsaI 374 GGTCTC GGTCTC(1/5) BsaWI 1107 TCCGGA [AT]^CCGG[AT] BseAI 1107 TCCGGA T^CCGGA BsiHKAI 763 GAGCTC G[AT]GC[AT]^C BsmFI 230 GTCCC GGGAC(10/14) Bsp1286I 763 GAGCTC G[AGT]GC[ACT]^C BspEI 1107 TCCGGA T^CCGGA BspLU11I 875 ACATGT A^CATGT BsrBI 1020 GAGCGG CCGCTC(-3/-3) DraI 813 TTTAAA TTT^AAA EcoT14I 1150 CCTTGG C^C[AT][AT]GG FauI 1022 GCGGG CCCGC(4/6) Fnu4HI 305 GCAGC GC^[ACGT]GC HapII 1108 CCGG C^CGG Hin1I 1054 GACGTC G[GA]^CG[CT]C HpaII 1108 CCGG C^CGG HphI 1079 TCACC GGTGA(8/7) ItaI 305 GCAGC GC^[ACGT]GC KpnI 730 GGTACC GGTAC^C MroI 1107 TCCGGA T^CCGGA MspI 1108 CCGG C^CGG PleI 1071 GACTC GAGTC(4/5) PshBI 1174 ATTAAT AT^TAAT PstI 1010 CTGCAG CTGCA^G SacI 763 GAGCTC GAGCT^C SexAI 370 ACCTGGT A^CC[AT]GGT SmlI 521 CTCAAG C^T[CT][GA]AG StyI 1150 CCTTGG C^C[AT][AT]GG SwaI 812 ATTTAAAT ATTT^AAAT TseI 305 GCAGC G^C[AT]GC XbaI 823 TCTAGA T^CTAGA XcmI 299 CCAACAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 694 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 9 GCGG CCGC(-3/-1) 1022 GCGG AfaI 626 GTAC GT^AC 731 GTAC AflIII 341 ACGTGT A^C[GA][CT]GT 875 ACATGT AvaII 229 GGTCC G^G[AT]CC 601 GGTCC BalI 489 TGGCCA TGG^CCA 638 TGGCCA BanI 526 GGCACC G^G[CT][GA]CC 730 GGTACC BsmI 51 GCATTC GAATGC(1/-1) 1103 GCATTC BstYI 87 GGATCC [GA]^GATC[CT] 557 AGATCC EaeI 489 TGGCCA [CT]^GGCC[GA] 638 TGGCCA EcoRI 194 GAATTC G^AATTC 412 GAATTC MflI 87 GGATCC [GA]^GATC[CT] 557 AGATCC MluNI 489 TGGCCA TGG^CCA 638 TGGCCA MscI 489 TGGCCA TGG^CCA 638 TGGCCA MslI 102 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 996 CATGCTTGTG MwoI 305 GCAGCCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 610 GCTTCCGTCGC NspI 875 ACATGT [GA]CATG^[CT] 995 ACATGC PpuMI 228 AGGTCCC [GA]G^G[AT]CC[CT] 600 GGGTCCT PshAI 223 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1008 GACTGCAGTC RsaI 626 GTAC GT^AC 731 GTAC SfcI 533 CTATAG C^T[GA][CT]AG 1010 CTGCAG TfiI 134 GATTC G^A[AT]TC 271 GATTC Tsp45I 286 GTCAC ^GT[GC]AC 1028 GTCAC TspRI 883 CACTG CA[GC]TG[ACGT][ACGT]^ 936 CAGTG XhoII 87 GGATCC [GA]^GATC[CT] 557 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AcsI AluI AlwI ApoI BfaI BpmI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FokI HaeIII HinfI MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI Tru9I Tsp509I