Restriction map for
pFN21AE9548
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrFI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MflI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspI StuI SwaI Tru9I
Tth111I XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 862 GTCTAC GT^[AC][GT]AC
AcyI 1121 GGCGTC G[GA]^CG[CT]C
Asp700I 327 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 891 GAGCTC G[AT]GC[AT]^C
AvaI 1006 CTCGAG C^[CT]CG[GA]G
AviII 191 TGCGCA TGC^GCA
BanII 891 GAGCTC G[GA]GC[CT]^C
BcnI 688 CCCGG CC^[GC]GG
BpmI 672 CTGGAG CTGGAG(16/14)
BsaHI 1121 GGCGTC G[GA]^CG[CT]C
BseRI 580 CTCCTC GAGGAG(10/8)
BsiHKAI 891 GAGCTC G[AT]GC[AT]^C
BsoBI 1006 CTCGAG C^[CT]CG[GA]G
Bsp1407I 902 TGTACA T^GTACA
BsrBI 625 CCGCTC CCGCTC(-3/-3)
BsrDI 378 CATTGC GCAATG(2/0)
BsrGI 902 TGTACA T^GTACA
BssHII 678 GCGCGC G^CGCGC
DraII 4 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 4 GGGACCT [GA]G^G[ACGT]CC[CT]
FspI 191 TGCGCA TGC^GCA
HaeII 225 GGCGCT [GA]GCGC^[CT]
Hin1I 1121 GGCGTC G[GA]^CG[CT]C
HincII 1042 GTCAAC GT[CT]^[GA]AC
HindII 1042 GTCAAC GT[CT]^[GA]AC
MaeII 772 ACGT A^CGT
MslI 342 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 688 CCCGG CC^[GC]GG
PaeR7I 1006 CTCGAG C^TCGAG
PpuMI 4 GGGACCT [GA]G^G[AT]CC[CT]
PstI 724 CTGCAG CTGCA^G
PvuII 87 CAGCTG CAG^CTG
SacI 891 GAGCTC GAGCT^C
SbfI 723 CCTGCAGG CCTGCA^GG
SmlI 1006 CTCGAG C^T[CT][GA]AG
Sse8387I 723 CCTGCAGG CCTGCA^GG
SspBI 902 TGTACA T^GTACA
TaqI 1007 TCGA T^CGA
TfiI 950 GATTC G^A[AT]TC
Tsp509I 1191 AATT ^AATT
TthHB8I 1007 TCGA T^CGA
XhoI 1006 CTCGAG C^TCGAG
XmnI 327 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 630 CGCG CG^CG
679 CGCG
AlwI 167 GGATC GGATC(4/5)
1135 GGATC
AvaII 5 GGACC G^G[AT]CC
522 GGACC
BalI 398 TGGCCA TGG^CCA
884 TGGCCA
BbsI 559 GAAGAC GAAGAC(2/6)
747 GAAGAC
BglI 84 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
115 GCCTACCTGGC
BpuAI 559 GAAGAC GAAGAC(2/6)
747 GAAGAC
BsmAI 1063 GAGAC GTCTC(1/5)
926 GTCTC
BspMI 713 ACCTGC ACCTGC(4/8)
722 ACCTGC
BsrI 96 ACTGG ACTGG(1/-1)
1106 CCAGT
BssSI 1035 CACGAG CACGAG(-5/-1)
595 CTCGTG
BstUI 630 CGCG CG^CG
679 CGCG
BstXI 536 CCAAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
752 CCATGGTGCTGG
DraIII 534 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
592 CACCTCGTG
EarI 331 CTCTTC CTCTTC(1/4)
874 CTCTTC
FauI 566 GCGGG CCCGC(4/6)
1117 GCGGG
HapII 689 CCGG C^CGG
954 CCGG
HpaII 689 CCGG C^CGG
954 CCGG
Ksp632I 331 CTCTTC CTCTTC(1/4)
874 CTCTTC
MluNI 398 TGGCCA TGG^CCA
884 TGGCCA
MscI 398 TGGCCA TGG^CCA
884 TGGCCA
MspI 689 CCGG C^CGG
954 CCGG
MvnI 630 CGCG CG^CG
679 CGCG
PflMI 591 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
779 CCATCTGCTGG
PleI 1014 GACTC GAGTC(4/5)
1040 GAGTC
SfcI 648 CTACAG C^T[GA][CT]AG
724 CTGCAG
Van91I 591 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
779 CCATCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI BanI BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI HhaI HinP1I HinfI
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp45I TspRI XcmI